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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The purified protein was then reduced with tris(2-carboxyethyl)phosphine (TCEP, obtained from Sigma), a strong ...
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bioRxiv - Immunology 2019Quote: ... Protein aliquots were reduced with 5 mM tris-2-carboxymethyl phosphine (TCEP) buffer (Sigma Aldrich) and incubated for 1 hour at 60°C to reduce disulfide bonds ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich) rotating at 4 °C for 2 hr ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue lysates were precleared for 2 h with Protein A + G magnetic beads (EMD Millipore). Precleared lysates were then incubated with 5 μg of anti-Sox9 antibody (Abcam ...
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bioRxiv - Biophysics 2019Quote: ... GFP-PRC1 isoform 2 proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full length PRC1 was expressed for 4 hours at 18 °C after induction with 0.5mM IPTG ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was degraded by adding 2 µL 18.2 mg/mL Proteinase K (Millipore Sigma 3115887001) and incubating at 55°C for 1 hour ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 BA.1 nucleocapsid protein was subcloned into the pET46 vector (Novagen) with an upstream 6xHis tag and expressed in Rosetta2 pLysS E ...
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bioRxiv - Systems Biology 2022Quote: Single-cell protein lysates were digested with 2 ng of Trypsin (Sigma cat. nr. T6567) supplied in 1 μL of digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... and proteins denatured using SDS/PAGE loading buffer (bio rad) and 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by gel electrophoresis (NW04120BOX ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were expressed in Escherichia coli strain BL21(DE3) or Rosetta 2 (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Physiology 2023Quote: ... 15-35 µg protein per sample were denatured with 5 % v/v 2-mercaptoethanol (Sigma) in LDS sample buffer (Thermo ...
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bioRxiv - Biochemistry 2023Quote: ... TEV digested protein was concentrated to < 2 mL using an Amicon Ultra-15 concentrator (Millipore) and then purified by size exclusion chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... beads were incubated directly with 4X protein loading buffer (Li-Cor) with 2-Mercaptoethanol (Sigma). For digital PCR ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were stained for microtubule-associated protein 2 (Anti-MAP2, Rabbit; Millipore-Sigma; RRID: AB_5622). Following primary antibody incubation ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were stained for microtubule-associated protein 2 (Anti-MAP2, Rabbit; Millipore-Sigma; RRID: AB_5622). Following primary antibody incubation ...
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bioRxiv - Microbiology 2024Quote: ... Eluted protein was concentrated to 2 mL by centrifugation using Amicon Ultra centrifugal filters (Millipore) with a 50-kDa molecular weight cut-off (MWCO ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich 68957) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich 22790) ...
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bioRxiv - Cell Biology 2019Quote: ... 1:5000), mouse α-tubulin (B-5-1-2, 1:10000) and mouse α-Vinculin (hVIN-1, 1:5000) were obtained from Sigma-Aldrich. Rabbit α-Cyclin B1 has been described before [46] ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein staining solution (REVERT Total Protein Stain kit, LI-COR Inc., # 926-11010; Pyronin Y (Sigma-Aldrich # P9172)
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bioRxiv - Cell Biology 2021Quote: ... The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Neuroscience 2024Quote: ... Plasma concentrations of acylated ghrelin and leptin were determined by commercial ELISA kits according to the manufacturers’ instructions (Spi Bio #A05117.96 and Millipore #EZML-82K, respectively).
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bioRxiv - Microbiology 2021Quote: ... and Mouse IgE Single Plex Magnetic Bead Milliplex MAP kit (Millipore). Samples were diluted 1:12500 (IgA ...
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bioRxiv - Molecular Biology 2021Quote: ... and TNF-α using Milliplex Mouse Th17 Luminex kit (EMD Millipore) on a MagPix Luminex instrument ...
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bioRxiv - Cell Biology 2022Quote: ... the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Sigma) was used according to manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2023Quote: Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich, USA) was used and performed following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Utilizing the Duolink in situ orange PLA mouse/rabbit kit (Sigma), these two antibodies were spatially coincided with high precision (< 40 nm ...
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bioRxiv - Immunology 2019Quote: ... the strips were further incubated for 2 h with goat anti-mouse IgG (Sigma-Aldrich) diluted 1:500 or goat anti-feline IgG (Southern Biotech ...
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bioRxiv - Physiology 2020Quote: ... and either IgG (2 μl/sample; Anti-mouse IgG monoclonal Ab; Sigma-Aldrich; Cat#M9144) or PPARG antibody (10 μl/sample ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-α-tubulin (1:2,000; clone B-5-1-2, Sigma-Aldrich, cat. # T5168) and mouse anti-MIC2 (1:2500 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the membrane was stripped and re-probed with mouse tubulin-alpha ab-2 (Sigma-Aldrich), 1:5000 dilution.
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bioRxiv - Molecular Biology 2022Quote: ... 2 × 107 yeasts were opsonised in 1 mL RPMI 10% (v/v) mouse serum (Sigma) at 37 °C for 1 h ...
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bioRxiv - Genetics 2023Quote: ... 1 µg ml–1 natural mouse laminin and 2 µM arabinosylcytosine (Ara-C, Sigma-Aldrich). After 48 h ...
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bioRxiv - Microbiology 2024Quote: ... Relevant slides were incubated for 2 hours with mouse α-FLAG-M2 antibody (Sigma, F1804) in 3% BSA/PBS in a humid chamber at 37°C followed by 3X washes ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for protein detection: anti-Flag M2 mouse 1/1,000 (F3165, Sigma), anti-myc 9E10 mouse 1/1,000 (sc-40 ...
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bioRxiv - Neuroscience 2021Quote: ... and mouse monoclonal anti-glial fibrillary acidic protein antibody (G3893, Sigma-Aldrich, St Louis, MO, USA). Immunoreactivity to the antigen was visualized using Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Molecular Probes ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used were: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (1:400; Sigma) and goat polyclonal anti-ChaT (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to a PVDF membrane and incubated with mouse α- FLAG M2 monoclonal (Sigma) primary antibody ...
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bioRxiv - Immunology 2022Quote: ... and then intracellularly stained with Peridinin chlorophyll protein-Cy5.5-conjugated anti-mouse TNF-α (Millipore, Billerica) and phycoerythrin-conjugated anti-mouse IL-4 (eBioscience ...
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bioRxiv - Microbiology 2023Quote: ... incubated with mouse anti-HA mAb clone 12CA5 conjugated with protein G agarose beads (Sigma-Aldrich) for 16 h at 4°C ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Neuroscience 2024Quote: ... Staining was performed against glial fibrillary acidic protein (mouse anti GFAP−Cy3™, Sigma-Aldrich, C9205), Secondary antibody was anti-mouse Alexa Fluor 488 (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were transferred to nitrocellulose membranes (Amersham) upon which M2 Anti-FLAG mouse antibodies (Sigma) were used to visualize 3X-FLAG fusion peptides.
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bioRxiv - Microbiology 2020Quote: ... and 1 mM tris(2-carboxyethyl)phosphine (TCEP)] containing 1 SIGMAFAST protease inhibitor EDTA-free cocktail tablet (Sigma). Due to the presence of two remaining cysteines in PelXC232S ...