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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... the supernatant was discarded and 0.5 % Trypsin (Thermo-Fisher) containing 2 mg/ml DNase I (Sigma Aldrich) was added to the pellet ...
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bioRxiv - Biophysics 2022Quote: ... the growth medium was exchanged to differentiation medium containing DMEM supplemented with 2 % horse serum (HS, Sigma), 1 % penicillin-streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were glucose-starved for 60 minutes in seahorse assay base media containing 2 mM glutamine (Sigma). Glycolysis was measured upon glucose injection (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (resistance 2-3 MΩ) were filled with an intracellular solution containing (mM): 110 CsMeSO3 (Sigma), 4.6 MgCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were incubated in blocking solution containing 2 mg/ml bovine serum albumin (A7030, Sigma-Aldrich), 1% gelatin from cold water fish skin (G7765 ...
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bioRxiv - Cell Biology 2023Quote: Yeast cells were grown in synthetic defined complete (SDC) containing 2% (w/v) α-D-glucose (Sigma), 0.7% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... in PBS containing 2% FBS and 1x protease inhibitor cocktail (PIC Set III, Millipore Sigma, Cat. 539134). For each sample ...
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bioRxiv - Cell Biology 2024Quote: Cells were plated 2 days before imaging in medium containing 1 µM Cdk4/6i (PD-0332991, Sigma). Twenty-four hours later ...
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bioRxiv - Molecular Biology 2020Quote: ... sections of mouse heart were perfused for 1-2 min with 2ml of 2% TTC (Sigma-Aldrich Co., St. Louis, MO, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were eluted by adding 125 ng/ml FLAG peptide to the OG-containing buffer (Sigma-Aldrich) and incubating the beads for 30 min per round of elution ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the protein were pooled and concentrated using an Amicon with a 3 KDa cut-off (Millipore). The protein was conserved in 10 mM Tris-HCl pH7.63 and 33% glycerol ...
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bioRxiv - Neuroscience 2020Quote: ... Half fresh medium containing Amyloid β Protein Fragment 1-42 (50 nM; Sigma-Aldrich, St. Louis, MO, USA) or DMSO (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing pure protein were pooled and concentrated in a 3,500 MWCO Amicon-Ultra centrifugal filter (Millipore Sigma) at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fractions containing purified protein samples were concentrated using a 10 kDa Amicon centrifugal filter (Merck Millipore) and the elution buffer suspending the purified protein was replaced with EDTA (10 mM)-PBS (1x ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was extracted from 5⨯106 cells with Pierce RIPA buffer (Thermo, 89901) containing proteasome inhibitor (Sigma, P8340) and quantified using the Pierce BCA Protein Assay Kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... For protein detection: cell lysates were prepared in RIPA lysis buffer containing protease and phosphates inhibitors (Sigma-Aldrich) and Western blotting was performed as described (69) ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing the proteins were concentrated with Amicon® Ultra-15 Centrifugal Filters Ultracel®-100K (Merck-Millipore, Burlington ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing protein were pooled and concentrated in a 10K Amicon Ultra-4 spin concentrator (Millipore 234753) down to 500 µL ...
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bioRxiv - Biophysics 2023Quote: ... Lysate containing FLAG-tagged target protein was purified using ANTI-FLAG M2 Affinity Gel resin (Sigma-Aldrich, USA) and eluted with 3x FLAG peptide (APExBIO ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions containing desired protein were pooled and concentrated to 500 μL by ultrafiltration (Amicon Ultra-15, EMD Millipore), then passed over a size exclusion column (HiLoad Superdex 200 PG ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing purified protein were united and concentrated on Amicon-Ultra-15 10 K filtration units (Merck-Millipore, Darmstadt ...
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bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing the protein were pooled and concentrated in centrifugal concentrators (CLVG01230, Merck Millipore, Cork, Ireland) to a final concentration of 30 mg/ml for crystallization ...
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bioRxiv - Cell Biology 2022Quote: ... N-glycosylated proteins were enriched by incubating cell lysates containing protease inhibitor and 1,10-phenanthroline (Sigma-Aldrich, #131377) with 20µl concanavalin A Sepharose beads (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fractions containing the protein were pooled and concentrated with a 30 kDa cutoff Amicon centrifugal filter (Sigma UFC9030) and loaded onto a Superdex 200 size exclusion column equilibrated with the storage buffer (25mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Bound domain C was then detected using an HRP-conjugated anti-FLAG antibody (Sigma-Aldrich, St. Louis, MO) and Ultra-TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HA9 (inhibitor of the substrate binding domain of Hsp70) and brefeldin A (disrupts Golgi apparatus, Sigma-Aldrich, Merck) were added for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... the domain deletion constructs (APLF, APLFΔAD, APLFΔFHA) transfected ES E14 cells were treated with 10μM etoposide (Sigma #E1383) for 4hours ...
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bioRxiv - Bioengineering 2023Quote: ... cells were incubated with the primary antibody (anti-Htt raised against the Proline-rich domain (PRD) (MAB5492, Millipore) at a dilution of 1/500 in PBST for 2 h at RT ...
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bioRxiv - Cancer Biology 2020Quote: Reagents and kits included: Orlistat (Sigma-Aldrich, 96829-58-2), Myriocin ...
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bioRxiv - Cancer Biology 2022Quote: ... protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore) and and the Syngene Pxi4 imaging system (Ozyme) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins expression was detected using an enhanced chemiluminescence kit (EMD Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... Protein synthesis was measured using a DHFR assay kit (Sigma-Aldrich), which measures the oxidation of NADPH (60 mM ...
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bioRxiv - Physiology 2020Quote: ... Protein concentration was measured using a BCA assay kit (Sigma Aldrich) and spectrometry at absorbance 562nm using a Synergy H4 Hybrid Reader (Biotek) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined by Bradford assay kit (Sigma-Aldrich, B6916). Lysates were mixed with 5 × sample buffer (250 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... as measured by a BCA protein assay kit (Sigma-Aldrich, USA).
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bioRxiv - Physiology 2022Quote: ... Proteins were visualized using a chemiluminescence (ECL) kit (Millipore, MA, USA). Target protein expression was normalized to β-actin (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins in gel was visualized with sliver staining kit from Sigma. The gels over 100 KDa (SATB2 molecular weight ...
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bioRxiv - Plant Biology 2024Quote: ... Rubisco was labelled using an Atto594 protein labelling kit (Sigma-Aldrich), according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... For labeling an Atto 488 protein labeling kit (Sigma-Aldrich, 38371) and an Atto 647N protein labeling kit (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial pellet was lysed in 2 mL 1× BugBuster™ protein extraction reagent (Novagen), incubated by slow shaking for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins denatured using SDS/PAGE loading buffer (bio rad) and 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by gel electrophoresis (NW04120BOX ...
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bioRxiv - Biochemistry 2022Quote: WT and mutant Streptococcus pyogenes Cas9 proteins were expressed in E.coli Rosetta 2 (DE3) (Novagen) for 16 hours at 18 °C as fusion proteins with an N-terminal His6-MBP-TEV tag ...
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bioRxiv - Pathology 2022Quote: ... 15-30 μg protein per sample were denatured with 5 % vol/vol 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher) ...
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bioRxiv - Biophysics 2022Quote: ... The purified protein was then reduced with tris(2-carboxyethyl)phosphine (TCEP, obtained from Sigma), a strong ...
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bioRxiv - Immunology 2019Quote: ... Protein aliquots were reduced with 5 mM tris-2-carboxymethyl phosphine (TCEP) buffer (Sigma Aldrich) and incubated for 1 hour at 60°C to reduce disulfide bonds ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mgs of total protein were incubated with 20 μL of V5 agarose (Sigma-Aldrich) rotating at 4 °C for 2 hr ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue lysates were precleared for 2 h with Protein A + G magnetic beads (EMD Millipore). Precleared lysates were then incubated with 5 μg of anti-Sox9 antibody (Abcam ...
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bioRxiv - Biophysics 2019Quote: ... GFP-PRC1 isoform 2 proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full length PRC1 was expressed for 4 hours at 18 °C after induction with 0.5mM IPTG ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was degraded by adding 2 µL 18.2 mg/mL Proteinase K (Millipore Sigma 3115887001) and incubating at 55°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... and proteins denatured using SDS/PAGE loading buffer (bio rad) and 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by gel electrophoresis (NW04120BOX ...