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Citations for Millipore Sigma :
1001 - 1050 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... For live confocal imaging of dat:eGFP and dat:mito-RFP dopaminergic neurons larvae were mounted in 1.2% low-melting point agarose in E3 embryo media containing 160ug/ml Ethyl 3-aminobenzoate methanesulfonate (Tricaine MS-222; Sigma-Aldrich 886-86-2) Tricaine anesthetic ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS + 0.5% BSA + 0.1% sodium azide + 2% Normal Mouse Serum (NMS) and 2% FBS (Sigma, St. Louis, MO). Rat anti-mouse purified CXCR5 (2G8 ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... 70µg total protein per sample was reduced with tris-2-carboxyethyl phosphine (TCEP, Sigma) for 1 hour at 60°C at a final concentration of 5mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was diluted in Laemmli sample buffer with 5% 2-Mercaptoethanol (Sigma-Aldrich M6250) and denatured for 5 mins at 95°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 μg of purified protein was mixed with 2 mM of acetyl phosphate (Sigma) in 100 μl of phosphorylation buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein pellets were solubilised in 30 μl of 2 M Tris pH 8.0 (Sigma) / 70μl 3X SDS-PAGE loading buffer (60 mM Tris pH 6.8 (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were reduced using 5 mM tris (2-carboxyethyl) phophine hydrochloride (TCEP) (Sigma; 75259) for 10 min at 95° C and alkylated using 10 mM 2-iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were eluted twice with 2 μg/μl V5 peptide in water (Sigma-Aldrich) or 1 μg/μl 3x FLAG peptide (ApexBio ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... followed by TBST-Blotto containing two primary antibodies: monoclonal mouse anti-FLAG M2 (1:2,000 dilution, Sigma) and monoclonal rabbit anti-RNA polymerase beta (1:2,000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse tissues were pulverized using pestle and mortar and homogenized in RIPA buffer containing protease inhibitors (Sigma), and 2% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2021Quote: Mouse eye cups were fixed for 1 h in PBS containing 4% (w/v) paraformaldehyde (Sigma-Aldrich) at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse lung tissue was homogenized in RIPA lysis buffer containing commercially available protease and phosphatase inhibitors (Sigma) after RA and HOX treatment ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma), and other primary antibodies were used at a 1:1000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... A lentivirus plasmid vector containing an shRNA insert not targeting human and mouse genes (SHC002, Sigma-Aldrich) was used as a non-target control (shCTL).
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bioRxiv - Physiology 2023Quote: ... followed by overnight incubation at 4°C in blocking buffer containing mouse anti-vimentin antibody (Sigma, MAB3400) diluted 1:2000 ...
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bioRxiv - Microbiology 2023Quote: The Mouse Monoclonal Antibody Isotyping Reagents Kit (Sigma-Aldrich®) was used for the evaluation of IgG1 and IgM isotypes ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... or a monoclonal mouse anti-tubulin antibody (Sigma-Aldrich, Clone B-5-1-2). Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary antibodies mouse anti-double phosphorylated ERK1/2 (Sigma-Aldrich, #M8159, 1:200) and mouse anti-acetylated α-tubulin (clone 6-11B-1 ...
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Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... mouse ascites MAb B-5-1-2 α-tubulin 1:200 (Sigma-Aldrich #T5168); rat TgEB1 1:100 (Chen and Gubbels ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used: mouse anti-NMNAT2 (WH0023057M1 Sigma, 2 µg/ml), mouse anti-NAMPT (clone OMNI 379 ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Clone B-5-1-2) (1:1000, Sigma, Cat# T5168), and mouse anti-PyCSP (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... and mouse anti-α-tubulin 1:10,000 (monoclonal B-5-1-2, Sigma-Aldrich). To image the blot the secondary antibodies goat anti-rabbit IgG IRDye 800CW (LI-COR ...
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bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
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bioRxiv - Neuroscience 2023Quote: ... each mouse received an intraperitoneal injection of 2% Evan’s blue dye (Sigma E2129-10G) dissolved in 0.9% saline solution ...
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bioRxiv - Developmental Biology 2023Quote: ... MPM-2: Mouse antibody (isotype - IgG1) from Sigma-Aldrich (05-368, St. Louis, Missouri) was used at a dilution of 1:500 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was collected and centrifuged at 900g for 5mins and GLP-1 secretion over 1h treatment with GYP was measured using a total GLP-1 ELISA kit (Millipore, UK) according to manufacturer’s specifications.
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bioRxiv - Pathology 2024Quote: The content of human IgG was quantitated both in the sera and aortic (tissue) homogenates from rats treated with chP3R99 or hR3 mAbs by a commercial ELISA kit (Sigma-Aldrich). Similarly ...
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bioRxiv - Immunology 2020Quote: ... The polyreactivity of hybridoma-obtained IgA was tested by adding them to ELISA plates that were coated with common antigens dissolved in PBS before coating ELISA plates: Lipopolysaccharide (Sigma-Aldrich; L4391), double stranded DNA (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: ... The protein amount of the resuspended membrane fractions was quantified by Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) and equilibrated between different samples ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich, St. Louis, Missouri United States) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... solium larval extracts were assessed for protein concentration using a BCA or Bradford protein assay kit (Sigma-Aldrich), respectively.
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bioRxiv - Cancer Biology 2023Quote: ... the protein concentrations were measured using a standard BCA assay with Pierce™ BCA Protein Assay Kit (Sigma). 30 μg of proteins was loaded into a pre-cast gel (Novex™ 16% Tricine Gels ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Immunoblotting was performed with anti-Protein A mouse monoclonal primary antibody (SPA-27, Sigma Aldrich) or anti-LukS-PV mouse monoclonal primary antibody (IBT) ...
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bioRxiv - Neuroscience 2021Quote: ... Puromycin-labeled proteins were identified using the mouse monoclonal antibody (1:10,000, Millipore, catalog MABE343). Protein synthesis levels were determined by analyzing total lane density from 10 kDa to 250 kDa ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibody against HCoV-OC43 nucleocapsid (NP) protein was purchased from Millipore (Cat.No. MAB9012). Rabbit polyclonal antibody against human LY6E was obtained from proteintech (Cat.No ...
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bioRxiv - Microbiology 2019Quote: ... PVDF membrane-bound proteins were first probed with mouse anti-HA (1:3000; Sigma, H3663) and rabbit anti-GAPDH (1:5000 ...