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Citations for Millipore Sigma :
9951 - 10000 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was submerged in secondary antibody solution (TBS-T + 5% BSA + 1:3000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody (Sigma-Aldrich) and incubated for two hours at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: All transgenic mouse lines used in this project are listed in (Extended Data Table 1) together with their respective Tamoxifen (TAM) (Sigma, T5648) dosage ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse and rat kidneys were fixed by perfusion with 50-60 mL of 4% paraformaldehyde in PBS (158127, Sigma-Aldrich), dehydrated and embedded in paraffin at 58C ...
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bioRxiv - Cell Biology 2023Quote: ... Receptor-mediated endocytosis was monitored in the proximal tubule of mouse kidneys PTs by measuring the uptake of β-lactoglobulin (L3908, Sigma). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... The sections were stained with primary antibodies diluted in 5% v/v horse serum over night at 4°C: mouse anti- glucagon antibody (1:1000, Sigma-Aldrich), guinea pig anti-insulin (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... This was followed by a 1 h incubation at room temperature with anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP; Sigma, A8924). Blots were developed using a Konica SRX101A X-Ray Film Processor.
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bioRxiv - Systems Biology 2023Quote: ... well-validated custom and commercial primary antibodies against proteins of interest were carefully selected and experimental parameters rigorously optimized to ensure high fidelity labeling: Connexin 43 (Cx43; mouse monoclonal antibody, Cat. # MAB3067, EMD Millipore Corp. ...
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bioRxiv - Immunology 2023Quote: Dendritic cells were collected from mouse spleens (Miltenyi 130-092-465) and co-cultured 5x105/mL with 50 ug OVA grade III (Sigma A5378) for 4h at 37C in 96-well U-bottom plates to load with antigen ...
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bioRxiv - Immunology 2023Quote: ... purified human neutrophil or mouse BM-derived neutrophils were stimulated with 10-7M N-formyl-Met-Leu-Phe (fMLF; Sigma-Aldrich) or 1 μM WKYMV (Phoenix Pharmaceuticals ...
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bioRxiv - Cell Biology 2023Quote: ... were dissected and immunostained as described previously.77 Dissected and fixed tissue was incubated overnight at 4°C with anti-Flag (mouse, 1:200; Sigma F1804) or anti-HA (mouse ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS + 0.5% BSA + 0.1% sodium azide + 2% Normal Mouse Serum (NMS) and 2% FBS (Sigma, St. Louis, MO). Rat anti-mouse purified CXCR5 (2G8 ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... we blocked membranes with 5% milk in PBS for 1 hour at room temperature and probed overnight with rocking at 4°C with the following primary antibodies: mouse anti-FLAG clone M2 (1/1000 dilution, Sigma # F1804), mouse anti-HA clone 16B12 (1/3000 dilution for HEK-293 cell lysates ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed with TTBS and incubated for 1 h at room temperature with isotype specific secondary antibodies anti-mouse IgG (1:10,000; Sigma-Aldrich, A4416) and anti-rabbit IgG (1:80,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was subsequently washed with MQ water followed by incubation for 20 min with 10 μl of anti-mouse 10 nm gold-labelled secondary antibody (1:10) (Sigma-Aldrich) and 10 μl of anti-rabbit 5 nm gold-labelled secondary antibody (1:10 ...
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bioRxiv - Physiology 2023Quote: ... TSH levels were determined using Luminex multiplex technologies with one-plex beads (Milliplex Mouse Pituitary Magnetic Bead Panel, Millipore, Burlington, MA). All hormone determinations were performed according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated for 48 hr at 4°C with mouse anti-TH antibody (1:2,000; Millipore, Burlington, MA) in 0.1 M PBS containing 2% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... MH27 mouse monoclonal antibody (from supernatent of cells provided by R. Waterston) was used with rhodamine-conjugated goat anti-mouse secondary antibody (Sigma-Aldrich) to stain fixed animals of different stages.
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bioRxiv - Genetics 2023Quote: ... 6H4 and 4H8 (1:100 for each) [71] and mouse anti-α tubulin DM1A conjugated directly to FITC (Sigma, 1:50). The secondary antibodies that were used were Cy3 ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with anti-calbindin-D28k mouse monoclonal antibody (1:2000 dilution by the blocking solution, C9848, Millipore Sigma) at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... a shRNA sequence based on 5’-GCCTAAATGGTCAAGGAGATA-3’ as the sense nucleotide corresponding to the 3’UTR of mouse mDia1 (NM_007858.4; Sigma-Aldrich, Cat# TRCN0000108685) was designed as an oligonucleotide with overhangs (s ...
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bioRxiv - Neuroscience 2023Quote: ... a sense sequence based on 5’-GCCCTAATCCAGAATCTTGTA-3’ corresponding to nucleotides 2473-2493 of mouse mDia3 (DIAPH2; NM_172493.2; Sigma-Aldrich, Cat# TRCN0000108782) was used to construct f(U6 ...
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bioRxiv - Neuroscience 2023Quote: ... Pelleted nuclei were resuspended in DPBS and incubated for 45 minutes with the mouse monoclonal antibody against neuronal nuclear marker NeuN conjugated to AlexaFluor 488 (1:1000, MAB377X; Millipore, MA). Before sorting ...
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bioRxiv - Biochemistry 2023Quote: ... After washing the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, A2179) in PBST ...
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bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2% Triton X-100 diluted in PBS for 1 h before incubation with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-LCN-2 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-monoclonal PSD95 (1:3000, MAB1598) and synaptophysin (1:10000, MAB329-C) (Burlington, MA), mouse monoclonal anti-actin (1:10000, A5441) from Millipore Sigma. Primary antibody incubations were conducted overnight at 4 °C followed by incubation with the appropriate HRP conjugated secondary antibody for 1 h at room temperature ...
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bioRxiv - Genetics 2023Quote: Sperm analysis – Epididymal sperm were obtained by making small incisions in the mouse caudae epididymides placed in 1 mL of warm M2 medium (Sigma Aldrich), and the sperm were allowed to swim up for 10 min at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... Primary antibody was anti-acetylated α-tubulin mouse monoclonal clone 6-11B-1 (1:500 dilution, Sigma, T7451-200, lot #077M4751V). Secondary antibody was Alexa Fluor 488 donkey anti-mouse IgG (1:1,000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were incubated with primary antibody solution 1:8000 dilution of acetylated α tubulin mouse monoclonal antibody (Sigma Aldrich T7451) and a 1:125 dilution of ift88 rabbit monoclonal antibody (Abcam ab184566 ...
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bioRxiv - Genomics 2023Quote: ... Cells were grown on gelatin-coated plates under standard cell culture conditions (37°C, 5% CO2, humid) on a ‘feeder’ layer of SNLP mouse fibroblasts mitotically inactivated by Mitomycin-C (Sigma Aldrich). mESCs were routinely passaged at ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: The following antibodies were used for immunoblotting: The HA epitope was detected with a primary mouse monoclonal anti-HA (1:1,000; Sigma-Aldrich, H9658); the Myc epitope was detected with a primary mouse monoclonal anti-Myc (1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were electrophoresed on polyacrylamide gels transferred onto PVDF membranes and probed using the following antibodies: mouse anti-beta-Actin (Sigma #A1978), mouse anti-Vinculin (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and of PTMs (tyrosinated tubulin: YL ½ antibody, ab6160; rat monoclonal, Abcam; acetylated tubulin: T6793 antibody, clone 6-11B-1, mouse, Sigma-Aldrich; polyglutamylated tubulin ...
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bioRxiv - Cell Biology 2023Quote: ... Tubulin labeling was carried out with a mouse monoclonal anti-acetylated tubulin antibody (T6793-6-11B-1, Sigma-Aldrich, 1:250) and followed by either a goat anti-mouse IgG Cy3 (115-165-166 ...
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bioRxiv - Developmental Biology 2023Quote: ... pregnant female mouse with E14.5 embryos was intraperitoneally injected with 5-bromo-deoxyuridine (BrdU, Sigma #B5002; 50 µg/g body weight) 1 hour before collecting the embryos ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was blocked in 5% milk with TBS-0.1%Tween for 2 hours at room temperature on a rocker and then incubated with indicated primary antibodies overnight at 4°C (1:1000 mouse anti-degron (Sigma-Aldrich) or 1:5000 mouse anti-tubulin (ThermoFisher)) ...
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bioRxiv - Cancer Biology 2023Quote: ... HT-29 (ATCC, HTB-38), MDA-MB-231 (ATCC, HTB-26) and mouse ovarian surface epithelial cell line ID8 (Sigma, SCC145) were cultured as adherent monolayers in DMEM medium supplemented with 10% FBS (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 2 % SDS and 100 mM β- mercaptoethanol] at 60 °C for 45 min and reprobed with mouse monoclonal anti-β-actin antibody (0.4 μg/ml; Sigma Aldrich) overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then incubated overnight with primary antibodies against Yes-associated protein (YAP) (mouse monoclonal anti-YAP, 1:400, Sigma-Aldrich) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were washed three times with PBST for 5 minutes each before incubating with anti-mouse secondary antibodies (Sigma-Aldrich, F1804) for at least 60 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... CreERT2 activity was induced as previously described whereby P3 mouse pups were injected intraperitoneally with 100 μl of tamoxifen (Sigma, T5648) solution (3.5 mg/ml ...
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bioRxiv - Physiology 2024Quote: ... and proteins were detected by Western blot using monoclonal anti-Flag M2 antibody produced in mouse (1:5000 dilution; Sigma-Aldrich), rabbit anti-FKBP12 antibody (1:1000 dilution ...
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bioRxiv - Physiology 2024Quote: ... was incubated for 3 h at 4 °C with 1 μl of mouse anti-Flag M2 antibody or 3X Flag peptide (200 mg/ml; Sigma-Aldrich) on a rocking platform ...
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bioRxiv - Biophysics 2024Quote: ... isolated myofibrils were incubated for 1.5 hrs at 4°C on a rotator with 11 nM anti-α-actinin mouse antibody (A7811; Sigma-Aldrich), 5.5 nM Alexa-488 goat anti-mouse IgG (A11001 ...
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bioRxiv - Genomics 2024Quote: ... The following primary antibodies are used for the first target protein at the dilutions indicated: NeuN (1:500, A60, Mouse, Millipore MAB5374), pTDP43 (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were incubated for 2 hours at room temperature with monoclonal anti-β-actin HRP-conjugated mouse antibody (A3854, Sigma-Aldrich). Membranes were developed with Immobilon® Forte Western HRP Substrate (Cat ...