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Citations for Millipore Sigma :
9801 - 9850 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: Islets were transfected with either 50 μMol of a pool of PTGS2 small interfering (si)RNAs ON-Target plus Mouse Ptgs2 (19225) siRNA-SMARTpool (Dharmacon/Horizon Discovery, Cultek, Madrid, ES) or a scramble siRNA (Sigma-Aldrich) as previously described 25 ...
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bioRxiv - Biophysics 2020Quote: ... The cells were then washed with 1× PBS 3 times and immunofluorescence staining was performed using mouse anti-alpha tubulin primary antibody (T5168, Sigma Aldrich) at 1:1000 dilution ...
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bioRxiv - Evolutionary Biology 2020Quote: ... seminase(1:1000) [10,20,40] and mouse antibody against actin (as a loading control; Millipore Corp., cat no. #MAB1501MI at 1:3000) were used as primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit/mouse HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for 45 minutes at RT ...
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bioRxiv - Genetics 2021Quote: ... Some samples were labeled only with the mouse monoclonal DM 1A anti-a-tubulin (#T9026) from Sigma-Aldrich (St-Louis, MO), diluted 1:500 ...
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bioRxiv - Genomics 2021Quote: ... and CTCF-AID-eGFP19 mouse embryonic stem (mES) cells were cultured on plates pre-coated with 0.1% gelatin (Sigma-Aldrich, G1393) in Glasgow Modified Essential Medium (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... The pLenti pLKO-non-target shRNA control vector (SHC002) and two different pLenti-mouse shRNA vectors for FYN were selected from Sigma Aldrich MISSION® shRNA Vectors ...
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bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
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bioRxiv - Molecular Biology 2021Quote: ... Aly (Zhou et al. 2000) and TRAPα (Görlich et al. 1990) or mouse monoclonals against U1-70K (Sigma-Aldrich, clone 9C4.1), mAb414 (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... Then cells were stained with anti-mouse IgG Taxes red-conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACSAria II (BD ...
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bioRxiv - Immunology 2021Quote: Lentiviral particles encoding guide RNA and Cas9 were produced in HEK 293T cells using mouse Irf7 CRISPR lentiviral transfer construct targeting exon 7 (target ID #MM0000202587) of Irf7 (Sigma Aldrich). Lenti CRISPR universal non-target plasmid was used as a control (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Spore suspensions were then incubated for 1□h with 1:20 donkey anti-mouse IgG antibody coupled to 12-nm gold particles (Sigma G7777) in PBS–1% BSA for 1□hr at RT ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed 5 times with PBS/0.5% Tween-20 and 50µl of HRP-anti-mouse-IgA (Sigma-Aldrich, 1:1000) was added to each well ...
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bioRxiv - Immunology 2020Quote: ... The membrane was initially blocked with 5% skin milk with PBS buffer for 2 hours and after few PBS rinse the membrane was incubated with monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2020Quote: ... ES-E14Tg2A (E14) mouse embryonic stem (ES) cells (obtained from ATCC, Manassas, VA) were maintained in RESGRO (SCM001) culture media (EMD Millipore) on feeder-free ...
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bioRxiv - Immunology 2021Quote: ... and 2 ml Flt-3L-containing supernatant [produced from SP2/0 transfected cell line secreting mouse recombinant Flt-3L42 for 7 days at 37°C until activation with 100 ng/mL LPS (Sigma-Aldrich) 18 hours prior to the assays.
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bioRxiv - Immunology 2021Quote: ... Wells were incubated with serial dilutions of the serum samples and the bound total IgG specific antibodies were detected by Peroxidase-conjugated Goat Anti-Mouse IgG (Sigma-Aldrich). Finally ...
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bioRxiv - Cell Biology 2021Quote: H&E and Herovici staining of 8 μm thick paraffin mouse skin sections were processed as previously described (59) and sections were mounted in DPX mounting medium (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... The primary antibodies against β-Actin and P53 were directly conjugated to HRP and were used as follows: mouse monoclonal anti-β-Actin-HRP at a 1:50,000 dilution (A3854 from Millipore Sigma); and goat polyclonal anti-p53-HRP at a 1:3,000 dilution (HAF1355 from R&D systems) ...
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bioRxiv - Neuroscience 2020Quote: ... the nitrocellulose membranes underwent a 1h blocking step in Rockland buffer before 6h incubation with primary antibodies (for all antibody RRIDS, see Key Resources Table): mouse α-KCC2 (1:1000, Millipore), rabbit α-phospho-KCC2-S940 (1:1000 Aviva Systems Biology) ...
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bioRxiv - Immunology 2022Quote: ... fibroblasts and MCF 10A cells were incubated with the FITC bound anti-mouse IgG secondary immunoglobulin (Fc specific) (Cat # F4143, Sigma-Aldrich) secondary antibody for 2 h at room temperature away from light ...
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bioRxiv - Microbiology 2022Quote: ... and incubated with goat anti-mouse antibody conjugated to alkaline phosphatase (Sigma-Aldrich; A3688-1 MI; 1:10,000 1% BSA/PBS) for 2 hr at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were incubated at room temperature for 1 hour with horseradish peroxidase (HRP) conjugated goat anti-mouse (1:10:000 Sigma 4416) or donkey anti-rabbit (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.3% Triton for one hour at room temperature prior to incubation with the primary antibody mouse anti-GAD65 (EMD Millipore, MAB3551) at a concentration of 1:100 overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... for 1 h at RT and directly labelled overnight at 4°C with primary antibodies (1:400 mouse mAB for sarcomeric alpha-actinin [CloneEA-53, Sigma-Aldrich, A7811] and 1:200 rabbit pAB for cardiac Troponin T [Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... FLAG-tagged constructs were then immunoprecipitated with Dynabeads Protein G beads coupled to mouse anti-FLAG M2 mAb (Sigma #F3165, RRID:AB_259529) for 3 hours at 4°C with rotation ...
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bioRxiv - Molecular Biology 2022Quote: The following antibodies were used for ChIP against the indicated epitopes: anti-Rpb1 clone 8wG16 (mouse, monoclonal, Millipore # 05-952-I), anti-Rpb1-S2-P clone 3E10 (rat ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C in anti-NeuN primary antibody (1:5000; monoclonal-Mouse Cat# MAB377; Millipore, California USA) in PBSTx with 1% normal goat serum (NGS ...
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bioRxiv - Biophysics 2022Quote: ... FLAG- TOP3A-RMI1/2 or FLAG-DNA2 were labeled with QDs pre-incubated with a mouse anti-FLAG antibody (Sigma-Aldrich) on ice for 10 minutes prior to injection ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Biophysics 2022Quote: ... cells were permeabilized with 0.3% Triton X-100 in PBS and then incubated with anti-α-actinin mouse monoclonal antibody (1:100, A7732, Sigma-Aldrich) in CAS-Block reagent (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Anti alpha tubulin-FITC mouse mAb (#F2168) and 4’,6-diamidine-20-phenylindole dihydrochloride (DAPI, #10236276001) were purchased from Sigma Aldrich, Germany ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates collected were run in SDS-PAGE (30 μl loaded) and blotted with the following primary antibodies: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... and then immunoblotted with the following primary antibodies in TBST with 5% milk at 4°C overnight: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Genomics 2022Quote: ... ChIPs were performed at 4 °C overnight with 10 μg of antibody in a final volume of 250-μl block solution (0.5% BSA (w/v) in PBS): mouse anti-H3K27ac monoclonal (Millipore # 05-1334), rabbit anti-H3K4me2 polyclonal (Millipore # 07-030) ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then washed with PBS-T and incubated with anti-rabbit or anti-mouse peroxidase-labeled antibodies (1:10000, Sigma-Aldrich). After extensive washing with PBS-T ...
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bioRxiv - Immunology 2022Quote: ... Immunized mice were administered Pertussis toxin intravenously (iv) through the tail vein (250 ng/mouse: Sigma Aldrich, St. Louis, MO, USA) in a final volume of 100 µL on the day of immunization and 48 hours later ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed and probed with either goat anti-mouse horseradish peroxidase or goat anti-rabbit horseradish peroxidase (Sigma-Aldrich) at a dilution of 1:10,000 for 1 h (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2022Quote: ... or a concentration of 50 nM for IMAC-purified DARPins) binding to CagI was performed using a mouse-anti-FLAG M2 monoclonal antibody (dilution 1:5000; Sigma, F1804) as primary and a goat-anti-mouse antibody conjugated to an alkaline phosphatase (dilution 1:10,000 ...
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bioRxiv - Plant Biology 2022Quote: ... Total NUC/GEN polypeptide abundance was detected by probing duplicate blots with a mouse anti-GFP antibody (#11814460001; Sigma Aldrich (Roche), Darmstadt ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hour at room temperature and subsequently incubated in the blocking buffer containing rabbit anti- SRGAP2 Nter to label SRGAP2B/C and SRGAP2A (1:1000, described in (12) and mouse anti- GAPDH (1:5000, Sigma #G8795). Antibodies were incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The following commercial primary antibodies were used: rabbit anti-Olig2 antibody and mouse anti-panNav mAb (clone K58/35) purchased from Sigma-Aldrich Chimie ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at room temperature with a combination of 2 different primary antibodies: a mouse monoclonal anti-PCNA antibody (1:500; Sigma-Aldrich; catalogue number P8825 ...
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bioRxiv - Neuroscience 2022Quote: ... Concentrations of RMAs in the collected blood were determined using the standard curves generated by the luciferase assays conducted on the normal mouse serum (Sigma-Aldrich) added with RMAs at known concentrations ...
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bioRxiv - Neuroscience 2022Quote: ... commissural neurons were isolated from E12 mouse dorsal spinal cords and plated on acid-washed coverslips coated with poly-D-lysine (Sigma, #P6407) and 2 μg/ml N-Cadherin (R&D ...
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bioRxiv - Neuroscience 2022Quote: ... We purchased a MISSION shRNA vector library encoding the microRNA-adapted shRNA targeting mouse Inka1 (Sigma-Aldrich, St. Louis, MO, USA). Among five shRNA clones (TRCN0000269004 ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were subjected to the following primary antibodies overnight at 4°C according to the experiment: mouse monoclonal anti-β-tubulin III (1:5000; Sigma) or mouse monoclonal anti-actin (1:4000 ...