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Citations for Millipore Sigma :
9851 - 9900 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (clone PAb416, Sigma Aldrich - Saint-Quentin-Fallavier France) diluted 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa 568-conjugated goat anti-rabbit IgG (1:500 dilution, A11011, Thermo-Fisher) or Alexa 568-conjugated rabbit anti-mouse IgG (1:500 dilution, A9044, Sigma-Aldrich) for 2 h at room temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... slices were incubated for 24-48h at 4°C with mouse anti-Neurophysin I (1:2000, Merck Millipore, marker for Oxytocin), rabbit anti-vasopressin (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were incubated with a mouse monoclonal antibody recognizing the C-terminal end of connexin 43 (Cx43) (Sigma Aldrich, Cat# MABT901) at a 1:100 dilution in 5% BSA-PBS-T overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Total RNA extraction (from BM-MSCs, mouse osteoblasts, RAW 264.7 cells, and differentiated osteoclasts) was obtained using TRI-Reagent (Sigma-Aldrich #T9424), following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrows were harvested by flushing mouse femurs and tibiae with ice-cold Sort Buffer (5mM EDTA, 25mM HEPES, 2% fetal bovine serum (FBS, SIGMA, #F2442) in 1× PBS (GIBCO ...
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bioRxiv - Cell Biology 2024Quote: Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA Probe anti-Mouse PLUS and anti-Rabbit Minus (Sigma-Aldrich). The incubation with all antibodies was carried out in a humidified chamber for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... (Supplementary Table 2) were obtained from the Merck CRISPR glycerol stock arrayed mouse sgRNA library available at WEHI ( Sigma Aldrich #MSANGERG).
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bioRxiv - Biochemistry 2024Quote: ... After incubation with horse-radish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit-HRP and goat anti-mouse-HRP, Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... then were incubated with the secondary antibody (Alexa-Fluor®-647-conjugated goat anti-mouse IgG H+L,1:2000 dilution) for 60 min at room temperature and counterstained with DAPI (Sigma-Aldrich) for another 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... lateral and medial) were each injected with 10 μl of either PBS or recombinant mouse GDNF (10 μg/ml, Sigma-Aldrich) using 31-gauge insulin syringes without damaging any innervating nerves ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 μg of lysates were incubated with 1 μg of mouse α-Flag M2 antibody (Sigma-Aldrich #1804; St. Louis, MO) in a final volume of 1 mL of immunoprecipitation buffer overnight at 4°C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: In vivo expression of OSKM in all reprogrammable mouse strains was induced by continuous or 3-4 days administration of doxycycline (Sigma, D9891) in drinking water (1 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... For staining the following primary antibodies were used: For labeling of nerve fibers mouse anti-beta-Tubulin III (1:200, Sigma, T8578); for pre-synaptic terminal staining rabbit anti-Synapsin ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse monoclonal anti-Flag (M2), mouse monoclonal anti-Actin (MAB1501, clone C4) and rabbit polyclonal anti-GST (G7781) were from Sigma-Aldrich. Rabbit polyclonal anti-FOXK1 (MNF ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were incubated in mouse anti-FasII (1:200, Developmental Studies Hybridoma Bank) and rabbit anti-myc (1:1000, Sigma-Aldrich) diluted in blocking buffer for 16 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.25% Triton X-100 for 30 min at RT and subsequently incubated in 0.1% Triton X-100 and 1% NS in PB with the following primary antibodies: mouse monoclonal anti-NeuN (1:1000; Millipore, #MAB377), anti-SMI32 (specific for non-phosphorylated neurofilament H ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 and 10% foetal calf serum in PBS] containing primary antibodies including: mouse anti-NeuN (1:200; Millipore, #MAB337); mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Genetics 2024Quote: The primary antibodies used in this paper were mouse anti-tubulin monoclonal antibody DM1A at 1:50 conjugated to FITC (Sigma-Aldrich), mouse anti-tubulin monoclonal antibody E7 at 1:200 (Developmental Studies Hybridoma Bank) ...
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bioRxiv - Developmental Biology 2024Quote: ... They were blocked in 1% BSA in PBT for 1hr at RT and incubated overnight at 4°C with the monoclonal mouse anti-FLAG M2 antibody (Sigma, F1804) diluted 1:500 in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2024Quote: ... at −20°C prior to quantification of glucagon secretion in both human and mouse islets using radioimmunoassay (Millipore-Sigma, GL-32K).
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bioRxiv - Cell Biology 2024Quote: ... at −20°C prior to quantification of glucagon secretion in both human and mouse islets using radioimmunoassay (Millipore-Sigma, GL-32K).
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bioRxiv - Immunology 2024Quote: ... and cytokine profiling post-challenge was performed on homogenate using a Milliplex MAP Magnetic Mouse Cytokine/Chemokine multiplex immunoassay (EMD Millipore).45
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bioRxiv - Microbiology 2024Quote: ... HRP-conjugated anti-mouse secondary antibody (57404) was purchased from SRL and anti-rabbit secondary antibodies were purchased from Sigma (A8275). Alexa Fluor 488 goat anti-mouse (A11001) ...
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bioRxiv - Microbiology 2024Quote: ... with the same combination of primary and secondary antibodies: mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and rabbit anti-HA tag antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... The following mixture of primary antibodies was used: a mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and a rabbit anti-HA tag antibody at a 1:50 dilution (Cell Signalling Technology) ...
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... and of PTMs (tyrosinated tubulin: YL ½ antibody, ab6160; rat monoclonal, Abcam; acetylated tubulin: T6793 antibody, clone 6-11B-1, mouse, Sigma-Aldrich; polyglutamylated tubulin ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was blocked in 5% milk with TBS-0.1%Tween for 2 hours at room temperature on a rocker and then incubated with indicated primary antibodies overnight at 4°C (1:1000 mouse anti-degron (Sigma-Aldrich) or 1:5000 mouse anti-tubulin (ThermoFisher)) ...
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bioRxiv - Cell Biology 2023Quote: ... Tubulin labeling was carried out with a mouse monoclonal anti-acetylated tubulin antibody (T6793-6-11B-1, Sigma-Aldrich, 1:250) and followed by either a goat anti-mouse IgG Cy3 (115-165-166 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were incubated with primary antibody solution 1:8000 dilution of acetylated α tubulin mouse monoclonal antibody (Sigma Aldrich T7451) and a 1:125 dilution of ift88 rabbit monoclonal antibody (Abcam ab184566 ...
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bioRxiv - Genetics 2023Quote: ... Primary antibody was anti-acetylated α-tubulin mouse monoclonal clone 6-11B-1 (1:500 dilution, Sigma, T7451-200, lot #077M4751V). Secondary antibody was Alexa Fluor 488 donkey anti-mouse IgG (1:1,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... 2 % SDS and 100 mM β- mercaptoethanol] at 60 °C for 45 min and reprobed with mouse monoclonal anti-β-actin antibody (0.4 μg/ml; Sigma Aldrich) overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL ...
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bioRxiv - Genomics 2023Quote: ... Cells were grown on gelatin-coated plates under standard cell culture conditions (37°C, 5% CO2, humid) on a ‘feeder’ layer of SNLP mouse fibroblasts mitotically inactivated by Mitomycin-C (Sigma Aldrich). mESCs were routinely passaged at ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher) ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-phosphoserine or anti-threonine antibody followed by 1:20000 anti-mouse antibody and detected using Luminata Forte Western reagent (Millipore, USA). Same protocol was followed for in vitro kinase assay using the mutated osbzip1.1 protein ...
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bioRxiv - Systems Biology 2023Quote: ... Thereafter the blocking buffer was discarded and the membranes were incubated with 1:1000 dilutions of mouse anti-FLAG M2 antibody (Sigma-Aldrich) in 1X TBST (overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded on 6-well cell culture plate wells at a density of ∼2 x 105 cells/well with 2 mL of the culture medium containing 103 units/mL ESGRO Recombinant Mouse Leukemia inhibitory factor (LIF) (Millipore #ESG1107) and 2i (1.0 µM PD0325901 Toris #4423 and 3.0 µM CHIR99021 Wako #038-23101) ...
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bioRxiv - Neuroscience 2022Quote: ... the following primary antibodies were incubated overnight at 4 °C in a humidified chamber: mouse anti-glial fibrillary acidic protein (GFAP) (1:500; Sigma-Aldrich), rabbit anti- laminin 1α (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (Cat#MAB-318, Millipore Sigma) at 1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2, 1:100, Millipore; mouse anti-GRIN2BB ...
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bioRxiv - Immunology 2022Quote: ... and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831; EMD Millipore) as described (83) ...
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bioRxiv - Biochemistry 2022Quote: ... The produced PSGL-1/mIgG2b were purified form the cell culture supernatants using goat anti-mouse IgG agarose beads (Sigma–Aldrich). Each enzyme (2 µM ...
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bioRxiv - Biochemistry 2022Quote: ... under agitation at room temperature for 20 min and re-probed with HRP-conjugated poly-clonal goat anti-mouse IgG Fc (1:5000 dilution, Sigma–Aldrich) for checking the integrity of the mouse IgG2b Fc domain of the fusion protein ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA (rabbit monoclonal from Cell Signaling Technology Inc., cat. no. 3724S; and mouse monoclonal from Sigma-Aldrich, cat. no. H3663), anti-myc (mouse monoclonal clone 9E 10 from the University of Iowa Hybridoma Bank) ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed by incubating 1 mg of the whole cell lysates (input) with M2 mouse anti-FLAG (F3165, Sigma Aldrich) and 50 µL of microMACS Protein G magnetic beads (Miltenyi ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for immunofluorescence (IF): mouse anti-KHC (clone H2; MAB1614; recognizes primarily KIF5C34) (1:100, EMD Millipore); goat anti-KLC1 (V-17 ...
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bioRxiv - Immunology 2023Quote: ... Staining was conducted in PBS / 1 % BSA (CHO cells and huPBMCs) or PBS / 1 % BSA / 1 % normal mouse serum (bovine cells) (Sigma-Aldrich) for 40 min at 4°C ...