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Citations for Millipore Sigma :
9701 - 9750 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Every 9th free-floating section was selected and stained with mouse anti-NeuN antibody (EMD Millipore, Billerica, MA, USA; 1:1000), which is a neuronal marker ...
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bioRxiv - Biophysics 2020Quote: ... A second incubation of 1 hour at room temperature was then performed with goat anti mouse Alexa Fluor 555 and anti-rabbit FITC conjugated (1:400, F7512, Sigma Aldrich). For all the labelling ...
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bioRxiv - Biophysics 2020Quote: ... permeabilized cells were blocked with 3 % BSA in PBS for 30 min at room temperature and then incubated with mouse monoclonal anti-alpha-tubulin antibody (T6199, clone DM1A, Sigma-Aldrich) diluted at 1:200 in 3 % (w/v ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibody staining was carried out by placing coverslips upside down onto a drop of primary antibody staining mix (mouse-anti-beta-tubulin, catalog no. T5293, Sigma-Aldrich, diluted 1:300 in PBS containing 2% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were used at the following dilutions: horseradish peroxidase coupled secondary antibodies (α-mouse and α-rabbit, Sigma, 1:20,000), anti-mouse Oregon Green (Molecular Probes ...
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bioRxiv - Cell Biology 2021Quote: Bone marrow extracted from the hind legs of one C57/BL6 mouse was resuspended in 10 ml complete RPMI 1640 media (Sigma, R8578) (10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... 2013), rabbit anti-Mad2 (Heinrich et al., 2013), rabbit anti-Mad3 (Heinrich et al., 2013) or mouse anti-tubulin (Sigma, T5168). Secondary antibodies were either anti-mouse or anti-rabbit conjugated to HRP (Dianova ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with horseradish peroxidase (HRP)-conjugated goat polyclonal anti-mouse IgG (1:5,000 dilution, EMD Millipore Etobicoke, Ontario, Canada). Supersignal West Femto enhanced chemiluminescence substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... of SPTAN1-kRas and SPTAN1-Raf1 were identified using the Duolink® proximity ligation assay (PLA) in situ red for mouse/rabbit antibodies (DUO92101, Sigma). THP-1 cells were fixed onto microscope slides and permeabilized as outlined in the ICC/IF section ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with primary antibodies diluted with TBS + 2% BSA + 0.1% Triton X-100 at 1:1000 (human anti-ACA, Geisel School of Medicine at Dartmouth) and 1:4000 (mouse anti-α-tubulin, Sigma) following the standard immunostaining protocol as described above ...
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bioRxiv - Biochemistry 2022Quote: ... and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804; rabbit anti-NF-145 – Millipore AB1987), followed by washing in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Then cells were stained with anti-mouse IgG Taxes red conjugated antibody and anti-human IgG FITC-conjugated antibody (Sigma, USA) for another 30 min then analyzed by FACS Aria II (BD ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were Immunoblotted with incubation of anti-Ahnak (rabbit polyclonal, RU2024, 1:5,000) or Anti-Gapdh (mouse monoclonal, MAB374, Millipore, 1:5,000) primary antibody followed by incubation of horseradish peroxidase-linked goat anti-rabbit or anti-mouse secondary antibody (1 ...
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bioRxiv - Neuroscience 2022Quote: ... a standard immunohistochemical protocol was used to enhance weak native fluorescence [mouse α-Cre recombinase primary antibody (1:1500, MAB3120, Millipore); biotinylated anti-mouse secondary antibody (1:250 Jackson lab) ...
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bioRxiv - Biochemistry 2022Quote: ... HRP-conjugated secondary antibody (HRP-link anti-mouse IgG, Cell Signaling, 1:10 000 dilution for C-terminal Parkin Ab and HRP-link anti-sheep IgG, Sigma, 1:10 000 dilution for N-terminal Parkin Ab ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins transferred to nitrocellulose membranes were probed with either affinity-purified polyclonal rabbit anti-Gli326 or goat polyclonal anti-Gli3 (R&D, AF3690) and mouse anti-vinculin (Sigma, V9264) and visualized with fluorescent secondary antibodies (LI-COR IRDye 800CW ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal antibody against Tamr1 (mAb 1D11), anti-Ta-p104 (Clone IC12, polyclonal mouse antiserum) and rabbit monoclonal anti-acetyl H4 (Sigma-Aldrich). Antibodies were diluted in PBS-1% BSA-0.3% Triton X100 (PBST) ...
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bioRxiv - Neuroscience 2021Quote: ... Neural dendrites were detected with the neurofilament marker anti-SMI 311 mouse monoclonal antibody (1:600, Millipore Bioscience Research Reagents, USA). The day after ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:200 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... the bound NoV capsid proteins were detected with mouse anti norovirus GII (1:1000; NativeAntigen) and by adding horseradish peroxidase conjugated with goat anti-mouse IgG (1:1500; Sigma Aldrich). Horseradish peroxidase activity was detected with a TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Cancer Biology 2020Quote: The following antibodies and dilutions for Western blot (WB) and immuno-fluorescence (IF) were used in this study: mouse monoclonal anti α-tubulin (clone DM1A, Sigma, T9026 ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-rabbit IgG, 1:10,000, Jackson ImmunoResearch, cat# 111-035-144; 80-25 kDa: goat anti-mouse IgG, 1:4,000, Millipore, 12-349) diluted in TBST for 2 h at 25 °C ...
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bioRxiv - Microbiology 2020Quote: Infected cells grown on coverslips were fixed with 4% paraformaldehyde and permeabilized/blocked with a Triton-X100 (0.2%)/BSA (3%) solution and then incubated with mouse anti-Flavivirus Group Antigen 4G2 (Millipore, Burlington, MA), mouse anti-alphavirus capsid (kindly provided by Dr ...
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bioRxiv - Neuroscience 2020Quote: ... in TBS with 0.4 % Triton X-100 and incubated with a mouse anti-GFAP antibody (1:500, clone G-A-G, Sigma: G 3893) in blocking solution overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), guinea pig anti-NeuN (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with TBS-T (3 times for 10 min) and incubated with horseradish peroxidase-conjugated secondary antibodies anti-mouse (Millipore, #AP308P), or anti-rabbit (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... in TBST for 1 h at room temperature (RT) and incubated with primary antibodies against β-actin (mouse, 1:50,000, Sigma, A5316) and Myosin Light Chain 2 (rabbit ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated overnight at 4°C with an anti-GFP (ROCHE Anti-GFP, from mouse IgG1K, Millipore Sigma) (1:1000) ...
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bioRxiv - Neuroscience 2021Quote: ... the Cux2-creERT2;Ai163 mouse was fully anesthetized in 5% isoflurane and transcardially perfused with phosphate-buffered saline (P5493, Sigma Aldrich) followed by 4% paraformaldehyde (P6148 ...
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bioRxiv - Microbiology 2020Quote: The mouse Rab GTPases shRNA library (317 shRNAs) was extracted from The Mission mouse shRNA library generated by The RNAi consortium (Sigma Aldrich) and prepared as described before (Shi et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight at RT with a combination of two primary antibodies: a mouse monoclonal anti-PCNA (1:500; Sigma-Aldrich; catalogue number P8825 ...
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bioRxiv - Microbiology 2021Quote: ... Membrane was then washed 3x for 10 min with TBST and subsequently incubated with the alginate antibody (Sigma, monoclonal, anti-mouse) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then added to the Protein G sepharose beads coupled to either HA 12CA5 mouse monoclonal IgG (Sigma Aldrich) or anti-Ty1 (BB2 ...
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bioRxiv - Neuroscience 2020Quote: ... and manual application of 100μl of directly biotinylated mouse monoclonal IgG1 antibodies against Aβ (82E1, IBL, 0.2 μg/ml or 4G8, Millipore, 2 μg/ml) for 8 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Molecular Biology 2022Quote: Blots were washed three times for 15 minutes with PBS-T and probed 45 minutes with secondary antibodies (rabbit anti-mouse (Sigma A4312), 1:10000 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin in tris-buffered saline/0.1% [vol/vol] tween-20 (TBS-T) before probing overnight at 4°C with antibodies raised to the following targets: mouse anti-puromycin (Millipore-Sigma, MABE343), mouse anti-OC43 N (CoV antibody ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
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bioRxiv - Immunology 2022Quote: ... FLAG-DNAM-1-expressing human Treg cells were stimulated in complete RPMI-1640 culture medium at 37 °C for 5 days with plate-bound 0.2 or 1 μg/mL anti-human CD3 mAb (clone HIT3α) and either plate-bound 30 μg/mL mouse IgG1 isotype control or anti-FLAG mAb (clone M2, Sigma-Aldrich). The culture supernatants were harvested ...
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bioRxiv - Neuroscience 2019Quote: ... tissue was incubated in secondary antibody (Biotinylated horse anti-mouse IgG 1:500, BA-2001, Vector Laboratories, Burlingame, CA; biotinylated donkey anti-goat IgG 1:500, AP180B, Millipore-Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... the membranes were washed with TBST buffer for three times and subsequently incubated in 1:10,000 dilution of secondary antibody (anti-mouse IgG whole molecule conjugated with alkaline phosphatase) (A3562, Sigma-Aldrich, USA). The membranes were then washed with TBST buffer for three times and detected using AP detection kit (Bio-Rad ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were maintained on a layer of 40k per cm2 of mouse embryonic fibroblasts mitotically inactivated by Mitomycin C (Sigma M4287). Cultures were kept in incubators with 5% CO2 at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... Tween 20 with 3 % (w/v) skim milk powder and successively incubated with monoclonal mouse anti-T7 RNA polymerase antibodies (Novagen, Merck) and POD labelled goat anti-mouse antibodies (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were immunoblotted with HRP-conjugated Flag antibodies (Monoclonal ANTI-FLAG® M2-Peroxidase HRP antibody produced in mouse, A5892; Merck-Millipore), HRP-conjugated HA antibodies (12013819001 ...