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Citations for Millipore Sigma :
9751 - 9800 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... PVDF membranes were incubated with peroxidase-conjugated goat anti-rabbit IgG or goat anti-mouse IgG (1:5000, ZSGB-BIO, China) for 1 hours at room temperature and were visualized by chemiluminescence reagent (Millipore, USA). GAPDH served as the reference gene ...
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bioRxiv - Cell Biology 2019Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were incubated at room temperature for 1 h with Cy2/Cy3-conjugated anti-mouse or anti-rabbit secondary antibody (1:50; Millipore AP182C). After three washes in PBS ...
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bioRxiv - Microbiology 2019Quote: ... The TopA protein was subsequently detected using primary rabbit polyclonal anti-TopA antibodies and mouse secondary anti-rabbit IgG antibodies conjugated with alkaline phosphatase (Sigma Aldrich), according to the procedure described previously (Szafran et al. ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at 4 °C with mouse anti-SMI31/32 (to label neurofilament proteins; Millipore NE1022/NE1023; 1:1000) and chicken anti-myelin basic protein (to label myelin ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... and mouse monoclonal anti-DNA/Histona H1 antibody (1:100; catalog number 05-457/clone AE-4; Merck Millipore, MA, EUA). Alternatively ...
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bioRxiv - Neuroscience 2019Quote: ... cells were blocked-permeabilized in 5% gelatin from cold water fish skin and 0.1% triton X-100 for 1 h followed by incubation with primary antibodies (mouse anti-acetylated tubulin, and rabbit anti-Neurofilament heavy chain, Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... β-actin was also detected as a loading control using a 1:10,000 dilution of mouse anti-β-actin (Sigma, A5441). A 1:3,000 dilution of goat anti-mouse (Dako ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated with primary antibodies overnight at 4°C (rabbit anti-ORF2 mAbs at 1:1000; mouse anti-Flag M2 (Sigma F1804) at 1:2000) ...
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bioRxiv - Microbiology 2020Quote: ... or anti-gL (rabbit polyclonal) antibodies and detected using anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase (Sigma-Aldrich) and Pierce ECL-Western blotting substrate (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Neuroscience 2019Quote: ... at room temperature and incubated with anti-neurofilament (Chicken, 1:1000, Developmental Studies Hybridoma Bank, 3A10) and anti-NeuN (Mouse, Millipore, MAB377) primary antibodies in blocking buffer for 48 hrs at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... terminus or c.terminus anti-SPAG17 antibodies (previously described by Zhang and colleague, Zhang, Jones et al. 2005) and 1:1000 Mouse anti-Tubulin (Sigma #T6199) antibodies) ...
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bioRxiv - Genetics 2019Quote: ... Membranes were incubated overnight with agitation at 4 °C with 1:2000 monoclonal mouse anti-alpha-tubulin (Sigma-Aldrich; clone DM1A) and 1:1000 monoclonal mouse anti-Cas9 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse antibodies used in the study were raised against β-actin (Sigma-Aldrich, Clone AC-15, # A1978, 1:1000 for WB), GFP (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with the secondary antibody (anti-Mouse-HRP IgG, 1:10,000, Sigma-Aldrich, Saint Louis, MO, USA) diluted in TBS-T for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ChIP experiments were performed in AtT-20 cells as previously described (29) using 5 μg of antibody (anti-FLAG-M2, mouse monoclonal, Sigma F3165). ATACseq was performed as previously described (4) ...
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bioRxiv - Neuroscience 2020Quote: ... The glutamate-based intracellular solution for rupture-patch/Ca2+-imaging in mouse IHCs contained (in mM): 111 Cs-glutamate (C1251-500G, #SLBM4966V, Sigma, Germany), 1 MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was removed entirely and cells were suspended in 100 μl of the same TBS solution containing mouse monoclonal ANTI-FLAG M1 antibodies (Sigma, F3040) at a 1:500 dilution ...
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bioRxiv - Pathology 2020Quote: ... Membranes were then incubated with the corresponding secondary antibodies (rabbit anti-mouse IgG/HRP, Dako, #P0260 and goat anti-rat IgG/HRP, Sigma, #A9037) at a 1:5000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663 ...
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bioRxiv - Molecular Biology 2020Quote: Antibodies and dilutions for IF and Duolink were the following: mouse monoclonal anti-RPAP3 at 1:250 dilution (Sigma-Aldrich, SAB407956); polyclonal rabbit anti-TRBP at 1:100 dilution (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... The secondary solution was prepared with 1% non-fat milk in PBS-T using anti-mouse IgG (whole molecule)–alkaline phosphatase (AP) antibody produced in goat (Sigma-Aldrich) at a dilution of 1:3,000 ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight at 4 °C with primary anti-myosin heavy chains antibody (Monoclonal Anti-Myosin skeletal, Fast, Clone My-32, Mouse Ascities Fluid, M4276, Sigma-Aldrich) diluted (1:400 ...
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bioRxiv - Neuroscience 2019Quote: ... Robert Froemke and Moses Chao at NYU (1:200) and mouse anti-TH primary antiserum (MAB5986, Millipore, Burlington, MA; 1:2000) for 48 hrs at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Immunology 2019Quote: ... Each plate contained a standard curve with 2-fold dilutions of purified mouse IgG or IgA (Sigma Aldrich, St. Louis, MO) starting at 2,000 ng/mL ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary antibodies (rabbit anti-phospho-ERK, Cell Signaling Technology #9101, 1:1000; mouse anti-α-tubulin, Sigma-Aldrich #T9026, 1:5000) were diluted in Odyssey Blocking Buffer containing 0.2% Tween 20 ...
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bioRxiv - Cell Biology 2019Quote: Six-milliliter lysate of BL21 Star (DE3) expressing pET28a-FLAG-dDYNLL1 was incubated with 200µl mouse anti-FLAG magnetic beads (clone M2, Sigma-Aldrich, #M8823) at 4°C for 3 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Base media for the culture of mEpiLCs and mEpiSCs were supplemented with 20ng/mL Activin A from mouse (Sigma-Aldrich SRP6057) and 12ng/mL Fgf-2 from mouse (Sigma-Aldrich SRP3038) ...
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bioRxiv - Genomics 2019Quote: ... Cells were incubated with primary antibody diluted in the blocking buffer for 2 hours [alpha-tubulin mouse mAb (1:5000, Sigma T6199), Rad21 rabbit pAb (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... alkaline phosphatase-conjugated (1:10,000) or goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated (1:10,000) secondary antibody (Sigma, St. Louis, MO). Immunoreactive bands were detected by the addition of NBT/BCIP (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were then incubated overnight at 4°C in PBST-NGS containing mouse monoclonal anti-α-Tubulin-FITC antibody (Sigma-Aldrich) at a dilution of 1:200 ...
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bioRxiv - Genetics 2021Quote: ... was used for staining DNA and mouse anti-a tubulin monoclonal antibody DM1A (1:50) conjugated with FITC (Sigma, St. Louis) was used for staining microtubules ...
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bioRxiv - Genetics 2020Quote: ... and MILLIPLEX MAP Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 1-Cardiovascular Disease Multiplex Assay (#MCVD1MAG-77K-02, all from Millipore Sigma) were used to analyze cytokines and chemokines from mouse plasma ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1h and probed with the following primary antibodies: guinea-pig anti-Oddskipped (1/100, in house) and mouse anti-actin-HRP (1/20 000, Sigma A3854). After incubation with HRP-conjugated anti-guinea-pig secondary antibody (1/10 000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Reactions were initiated by the addition of a suspension of mouse liver microsomes (MLMs) (male CD-1 mice pooled, Sigma-Aldrich) or human liver microsomes (HLMs ...
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bioRxiv - Physiology 2020Quote: ... we incubated them with anti-rabbit PLUS and anti-mouse MINUS PLA probes (1 hour at 37°C) diluted in Duolink® incubation buffer (1:200; Sigma), followed by three washes with PBS ...
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bioRxiv - Pathology 2021Quote: ... cryosections were transferred to TBS and incubated for 1 h at 37 °C with anti-mouse PLUS and anti-Rabbit MINUS PLA probes (Sigma-Aldrich). Then ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µm sagittal sections were then cut on a cryotome, incubated first overnight with mouse anti-ankyrin-G (AnkG) (Rasband et al., 1999) (Sigma, #S8809) and rabbit anti-KCNQ2 (Cooper et al. ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ubiquitin proteins antibody (clone FK2; Enzo, New York, USA, Cat#BML-PW8810 and Sigma, Gothenburg, Sweden, Cat#04-263), guinea pig polyclonal anti-VGLUT1 (1:5000 ...
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bioRxiv - Physiology 2021Quote: ... and slides were also counter-stained with Cy3-conjugated anti-mouse alpha smooth muscle Actin SMA-α antibody (A2547 Sigma-Aldrich) at 1/500 ...
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-Syt (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... with IgG pull-down as controls (for ab194471, the control was a normal mouse IgG polyclonal antibody, 12-371 from Sigma-Aldrich; for ab31218 and PA5-27431 ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1hr and probed overnight with primary antibodies (Rabbit anti-SDMA, CST13222; mouse anti-vinculin, Sigma, RABBIT anti-PRMT5 (Abcam Ab31751). Blots were washed 3X with 1% TBST buffer and probed with secondary antibodies 680RD goat anti-Mouse and 800CW goat anti-Rabbit (LI-COR ...
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bioRxiv - Cell Biology 2020Quote: ... Intraperitoneal glucose tolerance test was performed by fasting overnight and injecting 2 g/kg D-glucose solution at 2 weeks after mouse beta cells were ablated with one high dose (150 mg/kg) of streptozotocin (Cat. No. S0130-1G, Sigma-Aldrich). Blood was collected in heparin-coated tubes at fed state ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4° C with the following primary antibodies in 1X PBS containing 2% Triton X-100 and 2% horse serum: mouse anti-NeuN (EMD Millipore), rat anti-Ki-67 (Invitrogen) ...