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Citations for Millipore Sigma :
951 - 1000 of 8173 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... protein G beads (Millipore) were used (2 hours ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells or MSC cells were transduced by overnight exposure to virus stocks and passaged at least five times under puromycin selection (Sigma; 6 µg/ml)
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 or PC-3 cells were transduced with virus-containing culture media supplemented with 10 µg/ml Polybrene (Millipore, Cat #TR-1003-G) for 24 hours and replaced with fresh growth media ...
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bioRxiv - Microbiology 2022Quote: ... variant was obtained from the RIVM (Netherlands) through European Virus Archive global and expanded on VeroE6 cells grown in DMEM (Sigma Aldrich, Cat #D6429) supplemented with 4% of FCS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... ½-log serially diluted influenza viruses in virus growth medium plus trypsin (DMEM, 0.1% BSA, 1 µg/mL TPCK-treated trypsin (Sigma, St. Louis, MO, USA)) were added to confluent monolayers of MDCK-SIAT1 cells (37 ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Microbiology 2023Quote: ... and 100 μL per well virus solution with or without 1μg/mL TPCK-treated trypsin (Sigma-Aldrich, St. Louis, MO, USA, Cat# 4352157) was added per well ...
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bioRxiv - Cancer Biology 2024Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...
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bioRxiv - Molecular Biology 2020Quote: ... double mutant KlGal80-K5A/R6Ap (NHKlGal80-K5A/R6Ap or IHGal80-K5A/R6Ap) and KlGal1p (NHGal1p) were expressed in Escherichia coli strain Rosetta (DE3)-pLys from pET-derived (Novagen) expression vectors (pETNHG80 ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysate (1 mg) from various yeast strains was used in immunoprecipitation with anti-Flag M2 magnetic beads (20 μl, Sigma) in a total volume of 1.5 ml of IP-Lysis buffer and incubated with end-over-end rotation for 4 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Each of the five strains were added to a separate well containing 90 μL of 1/10th ISP2 with 1.25% purified agar (Sigma-Aldrich). The surface of each well was imaged for 48 hours using a Nikon Eclipse Ti microscope with a 20x phase contrast lens ...
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bioRxiv - Plant Biology 2020Quote: ... 5mm mycelial blocks of different strains were placed on CM agar with 0.1 mg/mL CFW (Sigma-Aldrich, Shanghai, China), 0.2 mg/mL CR (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: 1dpi and 4dpi transgenic zebrafish larvae infected with fluorescent Mm strains were mounted in 0.8-1% low melting point agarose (Sigma-Aldrich) and imaged on a Leica TCS-SPE confocal on an inverted Leica DMi8 base and imaged using 20x or 40x objective lenses.
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bioRxiv - Immunology 2019Quote: ... Gram-negative strains were lysed with 10% SDS and Proteinase K (20 mg/mL) while Gram-positive strains were lysed with 15 mg/mL lysozyme (Sigma) and 5 mg/mL achromopeptidase (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... Pseudomonas syringae strains were grown at 28°C (with shaking at 200 rpm) in King’s B liquid medium (Sigma-Aldrich) containing appropriate antibiotics (King et al. ...
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bioRxiv - Immunology 2021Quote: ... or B6.SJL-PtprcaPepcb/BoyJ mice (B6 CD45.1, JAX strain 2014) by intraarticular injection of 400ug/10uL meBSA (Sigma-Aldrich) antigen into wrist ...
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bioRxiv - Systems Biology 2021Quote: ... coli strain and cultured the cells in M9 minimal medium (Difco) supplemented with 1/2 MEM amino acids solution (SIGMA) and 0.2% (w/v ...
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bioRxiv - Microbiology 2020Quote: All laboratory-adapted strains were maintained in human foreskin fibroblasts (HFF) cells in high glucose DMEM with sodium bicarbonate (Sigma) supplemented with 5% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 2 ml of each recipient and donor strains were combined (three mating pairs in total) and filtered through sterile 0.22 µm membranes (Merck Millipore, GSWP02500), which were incubated over TSA plates for 8 days at 22°C ...
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bioRxiv - Genomics 2021Quote: The high-quality genomic DNA was isolated from all three strains using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... strains were diluted to 0.005 and tested in biological triplicate in serial dilutions of tetracycline (Sigma Aldrich, St. Louis, MO), clarithromycin (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: 10 AB strain wild type embryos per condition per experiment were fixed in either 4% paraformaldehyde (PFA, Sigma Aldrich F8775) in 1X phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain MG1655-seeded NGM plates in the presence or absence of 1 mM auxin (IAA, indole-3-acetic acid, Sigma).
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bioRxiv - Microbiology 2022Quote: ... The strains were maintained on blood agar No2 (Oxoid, Hampshire, UK) supplemented with hemin (5 mg/mL) (Sigma Aldrich, Belgium), menadione (1 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... PCF trypanosomes (Lister strain 427) were cultured in modified SDM-79 medium with 10% heat-inactivated fetal calf serum (Sigma) at 27°C and 5% CO2 (60) ...
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was isolated from fresh bacterial cultures of the strains of interest using the GenElute Bacterial Genomic DNA Kit (SIGMA) according to manufacturers instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples from 24 mice of both strains (n = 3 per group) were separated by SDS-PAGE (8-20%) and transferred onto PVDF membranes (Millipore). Afterward ...
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bioRxiv - Microbiology 2020Quote: ... difficile strains were first grown on reduced Brain Heart Infusion (BHI) agar plates supplemented with 0,5% yeast extract (Sigma-Aldrich) and Clostridium Difficile Selective Supplements (CDSS ...
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bioRxiv - Systems Biology 2019Quote: ... Technical triplicate spectral data was searched against a customized reference proteome comprised by the reference proteome of the respective strain (see above) appended to the UPS2 fasta sequences (Sigma) using Proteome Discoverer 2.1 (Thermo) ...
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bioRxiv - Synthetic Biology 2021Quote: Chromatin fractionation of WT and SpH2A.WAt strains was performed as previously described [82] and fractions were analyzed by western blotting using anti-αtubulin (T6199, Sigma), anti-H3 (ab1791 ...
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bioRxiv - Molecular Biology 2021Quote: ... Obtained complemented strains were grown at 37°C for 16 h in BHI supplemented or not with 1 mM of IPTG (Sigma) until stationary phase was reached ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µL of stationary phase cultures of the complemented strains were spotted in BHI agar plates supplemented with Congo Red (Sigma) 25 µg.mL-1 and IPTG 1 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was first isolated from 40 dpf wild-type AB strain ovaries using TRI reagent (Cat. No. T9424; Sigma-Aldrich) and then cDNAs were synthesized with RETROScript Reverse Transcription Kit (Cat ...
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bioRxiv - Immunology 2020Quote: ... theta strain was suspended in Milli-Q water and mixed with an equal volume of 90% phenol solution (Sigma, 328111). The suspension was stirred continuously and maintained at 68 °C ± 2 °C for 30 min ...
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bioRxiv - Genetics 2020Quote: Paromomycin sensitivity was evaluated at 27°C by following over nine days the growth of four subcultures of wild-type and mutant mat+ strains on M2 medium supplemented (or not) with 500 μg ml-1 of paromomycin (Sigma). For each strain ...
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bioRxiv - Synthetic Biology 2022Quote: ... cerevisiae strains for preparation of competent cells were cultivated in YPD medium consisting of 10 g/L yeast extract (Merck Millipore), 20 g/L peptone (Difco ...
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bioRxiv - Microbiology 2022Quote: ... ljungdahlii strain and its derivatives was carried out in 125 mL serum bottles (Sigma-Aldrich, Merck Life Science, Gillingham, UK) with a working volume of 30 mL ...
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bioRxiv - Neuroscience 2022Quote: ... mice were weighed and received an intraperitoneal (i.p.) injection of sterile saline (SAL) or 5 mg/kg lipopolysaccharide (LPS, strain O111:B4, Sigma-Aldrich) dissolved in sterile saline14,66 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Yeast strains were grown in synthetic dropout (SD) media containing Yeast Nitrogen Base Without Amino Acids mix (Sigma-Aldrich Y0626) supplemented with 10 g/L glucose ...
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bioRxiv - Microbiology 2022Quote: ... strain CNRVC19247 was cultured in alkaline nutrient agar (casein meat peptone E2 from Organotechnie, 20 g; sodium chloride from Sigma, 5 g ...
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bioRxiv - Cell Biology 2023Quote: ... gondii RH ΔKU80Δhxgprt AtTIR1 and RH TatiΔKU80 strains were grown in Human Foreskin Fibroblasts (HFF) (ATCC, SCRC-1041) in DMEM media (Millipore Sigma) supplemented with 10% FBS [56 ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Microbiology 2024Quote: ... For the purpose of spore production and genetic manipulations AgN23 strain was cultivated on the solid medium Soya Flour Mannitol (SFM) medium (D-Mannitol (Sigma) 20 g/L ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strains were routinely grown in LB medium and MOPS/MES minimal medium supplemented with 0.01% casamino acids (CAA) (Sigma-Aldrich), 4 g/L glucose ...
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bioRxiv - Neuroscience 2023Quote: ... Adult male mice of the NOD-SCID strain (∼30–35 g) were administered 2 µl of 6-hydroxydopamine (6-OHDA, Sigma) dissolved in PBS at a concentration of 3.75 µg/µl with the addition of 0.01% ascorbic acid at the rate of 0.5 µl/min during a stereotaxic surgery ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of the cgs-3xFlag constructs in the resulting Atu strains was confirmed by Western blot using an anti-Flag antibody (Sigma).
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bioRxiv - Plant Biology 2023Quote: ... adjusted to an OD600 of 0.3 for cell death and electrolyte leakage assays and an OD600 of 0.1 for immunoblot assays (final concentration for each strain in a mixture) and were incubated with 100µM acetosyringone (Sigma-Aldrich) for 2-4 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cultures were then diluted 1/100 and grown at 370C for 12 hours in 96-well microtiter plates (16 wells for each strain) in the absence or presence of a range of concentrations of trimethoprim (Sigma). 600 nm OD data were collected at 10 min intervals ...