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Citations for Millipore Sigma :
801 - 850 of 7689 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the gradient-purified virus was subjected to buffer exchange using the Pur-A-Lyzer™ Maxi Dialysis Kit (20k MWCO) (Sigma) at 4°C overnight to replace the iodixanol with PBS.
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... reporter virus particle-containing supernatant was passed through a 0.22 µm Millex SteriFlip Vacuum-driven Filtration System (Sigma-Aldrich, Burlington, MA) before aliquoting and storage at −80°C ...
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bioRxiv - Plant Biology 2024Quote: ... Twelve-day-old quinoa plants were inoculated on their first pair of true leaves with 20 µl of the virus extracts by mechanical damage using silicon carbide (Sigma-Aldrich). Plants were kept in growth chambers ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Immunology 2024Quote: ... dissociated organoid cells were seeded in virus-containing organoid culture media with 5 μg/ml polybrene (EMD-Millipore TR-1003-G) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... virus was collected and used to infect NIH3T3 cells with 6 µg/ml polybrene (Sigma, St Louis, MO, USA; #TR-1003) for another 12 h ...
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bioRxiv - Biophysics 2022Quote: ... Protein G (Sigma) was coupled at 10 µg/mL in the presence of 10 mM sodium acetate buffer pH 4.5 at 30 µL/min for 300 seconds until ∼3500-4000 RU was immobilized ...
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bioRxiv - Microbiology 2021Quote: ... Protein G (Sigma) was covalently coupled following activation ...
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bioRxiv - Biophysics 2020Quote: ... Protein G (Sigma) at 10 μg/mL was coupled in the presence of 10mM sodium acetate buffer pH 4.5 at 30 μl/min for 300 seconds in various channels ...
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bioRxiv - Cell Biology 2019Quote: Protein carbonylation was determined by OxyBlot Protein Oxidation Detection Kit (Millipore). Wild-type animals were synchronized to the young adult stage ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A-Sepharose and Protein G-Sepharose were purchased from Sigma-Alderich ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was determined using the bradford protein assay (Sigma-Aldrich). Proteins were separated in 8% SDS-polyacryamide gel (PAGE-SDS ...
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bioRxiv - Plant Biology 2021Quote: ... Protein blots were stained with Ponceau S reversible protein stain (Sigma) to check transfer efficiency and equal protein loading ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentrations were determined using the BCA protein assay (Sigma-Aldrich) using bovine serum albumin as a standard ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Thirty microliters of protein G/protein A agarose beads (EMD Millipore) were washed with lysis buffer (containing protein inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration was determined by a BCA protein assay (Sigma). A total of 50 µg protein extract was mixed with 5× Laemmli loading buffer (50% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 50 μg were resolved on an SDS/PAGE denaturing gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 60 μg were resolved on an SDS/PAGE gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2024Quote: ... then removed the proteins with a 10K MWCO protein concentrator (Millipore) and quantified the resulting HOSCN solution (typically 3.5 to 4 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein quantification was performed with the BCA Protein Assay Kit (Millipore); for each sample ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
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bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
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bioRxiv - Bioengineering 2021Quote: Cells were cultured in the appropriate culture media and infected with S-pseudotyped virus in the presence of polybrene (Sigma; TR-1003) to a final concentration of 5 µg/ml.
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bioRxiv - Immunology 2019Quote: ... duplicate wells of a 12-well plate were seeded with 1 ml/well of the cell suspension (1 × 106 cells/ml) and virus was added in the presence of 6 μg/ml polybrene (Sigma-Aldrich, #107689) (multiplicity of infection of 25) ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-containing supernatant was filtered and used to infect WM115 cells in the presence of 1 μg/mL polybrene (Sigma-Aldrich, H9268). This was repeated with fresh viral supernatant 48 hours later ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL of each purified virus library were incubated with 100 μL of an endonuclease cocktail consisting of 1000U/mL Turbonuclease (Sigma T4330-50KU) with 1X DNase I reaction buffer (NEB B0303S ...
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bioRxiv - Microbiology 2022Quote: ... we concentrated ∼75 mL of virus to ∼3 mL (25x concentration) using an Amicon Ultra-15 Centrifugal Filter Unit (Millipore Sigma; UFC905008). All viral and lentiviral infections and transductions were done in the presence of 20 μg/mL DEAE-Dextran (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... beginning with a virus stock dilution of 1:5 in complete DMEM media as mentioned above supplemented with DEAE-Dextran (Sigma Aldrich #30461) at the final concentration of 40 mg /ml ...
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bioRxiv - Cancer Biology 2023Quote: ... were spin transduced with 10-20μl of the concentrated virus at 37°C at 2200g for 90 minutes with polybrene reagent (8-10μg/ml, Millipore Sigma, Billerica, MA). The cells were washed with PBS and resuspended in fresh RPMI media supplemented with 10% FBS with 1% penicillin and streptomycin 24 hours post-transduction ...
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bioRxiv - Biochemistry 2023Quote: ... C2C12 cells were seeded in a 6 cm dish and 105-106 virus particles were added dropwise to media containing 8 ug/mL Sequabrene (Sigma S2667-1VL) and 25 mM Hepes buffer ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Cell Biology 2023Quote: ... lentiviral expression plasmids were co-transfected with ΔNRF and VSVG (gifts from J. bear) virus packaging plasmids into HEK293T cells using 50 µg/mL polyethylenimine (PEI)-Max (Sigma-Aldrich Chemistry). Two days later ...
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bioRxiv - Genetics 2024Quote: ... The recipient cells were infected 48 hours post-transfection of HEK 293 cells with the virus supernatant in the presence of 8 μg/ml polybrene according to the manufacturer’s guidelines (Sigma-Aldrich, MO, USA). The cells transduced with the pLVX-Tight-Puro-FoxM1-dNdK were selected in 2 μg/ml puromycin (Gibco ...
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bioRxiv - Microbiology 2019Quote: 4 mL of each strain was grown to an OD600 of 0.3 and filtered through 0.22 µm nylon filters (Millipore GNWP04700). The filter was placed upside down in a 60 mm dish containing 1.2 mL pre-chilled quenching solution (40:40:20 Acetonitrile:methanol:H2O + 0.5% formic acid ...
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bioRxiv - Microbiology 2021Quote: ... Gold strain was performed in 800 ml 2xYT o/n at 37°C after induction with 0.5 mM IPTG (Sigma). Cells were harvested by centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... or catalytically-inactive BT4656S1_11 (in which Ser 77 is intact but prevents formation of formylglycine during expression in E.coli) were expressed in Escherichia coli strain TUNER (Novagen), and cultured to mid-exponential phase in LB supplemented with 50 μg/mL kanamycin at 37 °C and 180 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... The pdr5Δ strain was grown to mid exponential phase prior addition of either 50 μM MG132 (#C2211, Sigma-Aldrich) dissolved in DMSO or an equivalent volume of DMSO ...
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bioRxiv - Plant Biology 2021Quote: The bacterial strains used were pre-grown for 7 d in Tryptic Soy Broth 50 % (TSB 50 %, Sigma-Aldrich), and washed with 10 mM MgCl2 (series of centrifugations and removal of supernatant ...
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bioRxiv - Microbiology 2019Quote: ... encoding for amino acids D35-I221 of Bordetella pertussis strain Tohama I (UniProt_P04977) was cloned with NdeI and BamHI into pET15b (Novagen) allowing expression of an N-terminally HIS-tagged rPtxS1 (MGSSHHHHHHSSGLVPRGSHM-D35-PtxS1-I221) ...
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bioRxiv - Cell Biology 2021Quote: ... by mixing ~1×107 log-phase cells of each strain on a filter membrane (0.8 μm MCE Membrane Filter, MF-Millipore) on top of a YPD plate for 5 hours at 30 °C ...
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bioRxiv - Molecular Biology 2022Quote: In vivo expression of OSKM in all reprogrammable mouse strains was induced by continuous administration of doxycycline (Sigma, D9891) in drinking water (1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Cells were plated in 96-well plate in density of 1×106/mL in 200μL of medium and cultured at 37°C and 5% CO2 for 48h in presence of 5μg of lipopolysaccharide (LPS, isolated from E. coli strain 055:B5, Sigma, St ...
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bioRxiv - Microbiology 2022Quote: ... All bacterial strains and libraries were stored at - 80°C for long term storage in 25% sterile glycerol (Sigma). All library assays were performed in NEB10beta strain backgrounds (araD139 Δ(ara-leu)7697 fhuA lacX74 galK (Φ80 Δ(lacZ)M15 ...
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bioRxiv - Genetics 2019Quote: ... Conditional AID degron strains were grown in YPD or YPG and auxin (3-indoleacetic acid; Sigma Aldrich Cat. I3750) at a final concentration of 500µM to deplete AID-tagged proteins ...
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bioRxiv - Plant Biology 2019Quote: ... loading 60 μg protein from SN samples of WT and IM strains onto Amicon ultra centrifugal filters (0.5 mL, 30 kDa MWCO, Millipore) and digesting with 1.2 μg trypsin (sequencing-grade modified ...