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Citations for Millipore Sigma :
1051 - 1100 of 8173 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... on day 3 of activation (Fig. S1A) 250 µl of concentrated virus was preincubated with 8 µg/mL polybrene (EMD Millipore, TR-1003-G) for 30 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then infected with a GFP virus (SignaGen SL100268) at a concentration of 1 uL / 1 million cells supplemented with 1:1000 Polybrene Infection Reagent (Millipore Sigma TR-1003-G) for 90 minutes on a rotator in the 37°C incubator ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mL/well cell-virus suspension was seeded into 50 µg/mL rat tail collagen type I (Sigma Aldrich, St. Louise, MO USA) coated 6-well plates ...
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bioRxiv - Plant Biology 2020Quote: ... while the prey constructs cloned into the pDEST22 or pGADT7 were transformed into the strain Y8800 using the LiAc (Sigma-Aldrich) method (Schiestl and Gietz ...
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bioRxiv - Immunology 2021Quote: ... the strains were cultivated in Tryptic Soy Broth (TSB) (Difco Laboratories) supplemented with 1% of horse serum heat-inactivated (Sigma-Aldrich) for 24 hours at 37°C and 5%CO2 atmosphere [15] ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... the neurons of postnatal day 1–2 NMRI strain mice were grown 6 DIV on polyornithine-laminin (P3655 and CC095, Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141 ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to lysates from strain DPB215 +/– RNase and DPB221 or 20 μL of EZview Red Anti-HA Affinity Gel (Sigma E6779) was added to lysates from strain DPB215 +/– RNase and DPB220 ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial NAD levels were quantified from three independent mitochondrial preparations from both WT and xrn1-H41A strains using NAD/NADH Quantitation Kit (Sigma-Aldrich). Mitochondrial extract corresponding to10 μg of protein from both WT and xrn1-H41A were used to determine NAD levels as per instructions provided in the kit.
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bioRxiv - Plant Biology 2020Quote: Plasmids were transformed into Agrobacterium strain GV3101 (pMP90) by electroporation with selection on Luria-Bertani plates containing 50 μg/mL kanamycyin sulfate (Sigma-Aldrich) and 20 μg/mL gentamicin (Gibco) ...
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bioRxiv - Synthetic Biology 2020Quote: ... HEK239T cells (ATCC, strain number CRL-3216) used for part of this study were maintained in DMEM (Sigma-Aldrich or Gibco) supplemented with 10% FBS (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... Isolated actinomycetes strains were spot inoculated on a modified agar screening media with 1% (w/v) Avicel® PH-101 (Sigma) as a substrate for exo-β-1,4-glucanase and 0.2% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... cells were grown to an OD600nm of ∼0.3 and two different strains were mixed at equal parts in MM media containing 5 μg/ml of FITC-lectin (L9381, Sigma-Aldrich). After 15 min of incubation ...
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bioRxiv - Microbiology 2021Quote: ... ROS accumulation in host cells infected by different strains at 36 hpi was observed by staining the cells with DAB (Sigma-Aldrich) as described (48).
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bioRxiv - Microbiology 2020Quote: Wild type T7 phage (T7Select®) and host BL21 Escherichia coli bacteria strain were purchased from Millipore Sigma (Burlington, MA, US). To prepare a sufficient concentration of phage for experiments ...
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bioRxiv - Microbiology 2021Quote: Bacterial strains (listed in Table S2) were grown in Luria Bertani (LB; Carl Roth, Germany) or Müller-Hinton (MH; Sigma Aldrich, Germany) broth or DMEM GlutaMAX (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Yeast cell lysate from Rad6-FLAG strain (GMS488) was cleared by centrifugation and loaded into a column containing M2 anti-FLAG resin (Sigma-Aldrich). The sample was eluted with 100 μg/ml of 3X FLAG peptide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... and three old (5.5 months old) Nothobranchius furzeri (GRZ strain) male fish were sedated in 200mg/L of Tricain (Sigma-Aldrich, A5040), and then euthanized in 500mg/L of Tricain in system water ...
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bioRxiv - Microbiology 2022Quote: ... difficile strains were routinely grown in BHIS broth or on BHIS agar supplemented with 2-5 μg ml-1 thiamphenicol (Sigma-Aldrich) as needed [58] ...
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bioRxiv - Plant Biology 2021Quote: ... The regenerating plantlets were cocultivated with the agrobacterium strain GV2260 harboring the binary vector in liquid 0M51C medium containing 2% (w/v) sucrose and 100 μM acetosyringone (Sigma-Aldrich) at 22 °C under continuous white light for four days ...
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bioRxiv - Biochemistry 2021Quote: ... 10 µL of culture for each strain and dilution were plated to LB or LB supplemented with L-Rhamnose (Sigma-Aldrich) to a final concentration of 1% ...
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bioRxiv - Physiology 2021Quote: ... elegans were transferred into 24 well plates containing 1 mL M9 Buffer in quadruplicates for each strain and condition (three wells with sodium arsenite (Sigma-Aldrich) and one well M9 as control) ...
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bioRxiv - Microbiology 2022Quote: Aldehyde and D-lactate production in Salmonella strains were measured by the Aldehyde Assay Kit and D-Lactate Assay Kit (Sigma-Aldrich), respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Hepatocytes were infected with HBV virus GTC (PXB Strain PBB004, Lot; 20151109) at 5 genome equivalents per cell in DMEM inoculum medium containing 2% DMSO (Sigma-Aldrich), 4% PEG-8000 (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... The liquid handler was then used to transfer strains into glass-bottom 384-well microscope plates (Brooks Bioscience) coated with Concanavalin A (Sigma-Aldrich) to allow cell adhesion ...
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bioRxiv - Microbiology 2020Quote: ... HiaBD1-F and HiaBD1-R were used to amplify BD1 from NTHi strain R2866 genomic DNA using KOD hot-start polymerase (EMD Millipore) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The Human Respiratory syncytial virus (HRSV-A Long strain ATCC-VR26) was produced in LLCMK2 cells (ATCC CCL7) in EMEM supplemented with 2 mM L-glutamine (Sigma Aldrich), penicillin (100 U/mL) ...
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bioRxiv - Microbiology 2019Quote: NA activities of recombinant AIV strains were measured using the fluorogenic substrate MUNANA (2’-[4-Methylumbelliferyl]-alpha-D-N-acetylneuraminic acid; Biosynth and Sigma-Aldrich), as described previously [70] ...
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bioRxiv - Biochemistry 2019Quote: EcSerRS (from E. coli strain B ER2560) and SaSerRS (from S. aureus seg50 (1150)) were cloned into pET52b(+) vector (Merck Millipore, Germany) using the NcoI and SacI restriction sites allowing for the production of protein with a thrombin cleavable C-terminal His10-tag ...
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bioRxiv - Genetics 2020Quote: ... Deletion of 11 bp tandem repeats in lacZ was performed as described (Seier et al. 2011) with strain STL14025 For experiments involving 5-azacytidine (Sigma Aldrich) or formaldehyde (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: MERS-CoV and SARS-CoV-2 strains were obtained from different collaborators and passaged once in VeroE6 cells in DMEM (Sigma Aldrich) supplemented with 2% fetal bovine serum (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: JW18 Drosophila melanogaster cells with and without Wolbachia (strain wMel) were grown at 24 °C in Shields and Sang M3 insect media (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... Transformed YZGA515 strains were streaked and maintained on Synthetic Complete Medium (Dunham et al. 2015) supplemented with leucine drop out powder (Sigma Aldrich). To confirm proper transformation of yeast strains ...
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bioRxiv - Immunology 2021Quote: ... The Mb3601-EGFP fluorescent strain were derived by electroporation with an integrative plasmid expressing EGFP and selected with Hygromycin B (50 μg/mL) (Sigma, USA) as described in previously [33] ...
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bioRxiv - Microbiology 2021Quote: ... The initial bacterial inoculum 5⨯105 Colony Forming Unit (CFU)/mL for each strain cultured in Mueller Hinton Broth (MHB) (Sigma, Spain) was used in a 96-well plate (Deltlab ...
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bioRxiv - Microbiology 2020Quote: ... All Strain were maintained in 40 % glycerol, 60 % Lysogeny Broth (LB, 1 /L, 15 g Agar, 10 g Tryptone (Sigma-Aldrich), 5 g Yeast Extract(Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... media was aspirated and cells were exposed to 10/50 μg/mL Lipopolysaccharide (LPS; E. coli strain 0111:B4; Sigma-Aldrich) (MilliQ H2O vehicle ...
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bioRxiv - Immunology 2022Quote: ... Strains were streaked from frozen stock onto yeast extract peptone dextrose (YPD; 1% yeast extract, 2% peptone, 2% dextrose, Sigma-Aldrich) agar and left overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The resulting strains were stored in saline TSA broth (1.3 % NaCl) (Fluka, Buchs, Switzerland) with 20 % glycerol (Sigma-Aldrich, Darmstadt, Germany) at - 20 °C (Bashan et al. ...
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bioRxiv - Neuroscience 2022Quote: ... mouse pups were injected subcutaneously between the skapulae with 2 mg/kg body weight (BW) lipopolysaccharide (LPS; Escherichia coli strain O55:B5, Sigma Aldrich) or the equivalent volume of saline on postnatal day (P)2 ...
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bioRxiv - Microbiology 2023Quote: ... Typhimurium WT and ΔcspE strains were diluted 1:250 in LB with or without 200 μM 2,2-bipyridyl (Sigma-Aldrich, China). Cultures were incubated at 37°C at 180 rpm ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Breeding pairs of all mice strains (6 weeks old) were fed with a chow diet containing 0.012% TCS (Sigma-Aldrich, 72779) dissolved in vehicle (2ml DMSO + 10ml water ...
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bioRxiv - Microbiology 2022Quote: ... Secreted CPSs from each cryptococcal strain cells were collected and purified by ultrafiltration using an Amicon® system with a membrane cutoff of 10 kDa (Millipore). The effective diameter and the polydispersity of the secreted polysaccharide preparations were measured in a 1 mg/mL solution (weight per volume ...
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bioRxiv - Molecular Biology 2024Quote: In vivo expression of OSKM in all reprogrammable mouse strains was induced by continuous or 3-4 days administration of doxycycline (Sigma, D9891) in drinking water (1 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Plaque areas were expressed relatively to the mean value of those generated by the respective control strain treated with the vehicle (Dimethyl sulfoxide -DMSO-, D5879, Sigma-Aldrich) that was set to 100%.
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bioRxiv - Cell Biology 2024Quote: ... The liquid handler was then used to transfer strains into glass-bottom 384-well microscope plates (Brooks Bioscience) coated with Concanavalin A (Sigma-Aldrich) to allow cell adhesion ...
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bioRxiv - Cell Biology 2024Quote: ... mCherry-H2B was stably integrated at the act5 locus of Ax2-214 or GFP-Nup62 strains respectively and selected with 50 μg/mL HygromycinB (Sigma Aldrich) using available genetic engineering tools and electroporation protocols for D ...
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bioRxiv - Neuroscience 2022Quote: ... media was aspirated and cells were exposed to 10 or 50 μg/mL lipopolysaccharide (LPS) (E. coli strain 0111:B4; Sigma-Aldrich) (MilliQ H2O vehicle ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Clostridioides difficile strains were streaked from glycerol stocks and cultured using brain-heart infusion (BHI) agar or broth (Sigma Aldrich, USA) supplemented with 1g/L L-cysteine (Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: ... while plasmids-containing strains were grown in 1X EMM-LEU-URA minimal medium (EMM- LEU-URA Broth, Formedium, #PMD0810) supplemented with uracil (Sigma, #U750) (EMM- LEU) ...
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bioRxiv - Developmental Biology 2023Quote: ... from wing imaginal discs of Salm-3xFlag genotype (strain F1049, courtesy of Dr. Frank Schnorrer) using anti-Flag antibodies (Sigma-Aldrich). The samples from anti-Flag immunoprecipitated chromatin and controls were sequenced at the Centre for Genomic Regulation ...