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Citations for Millipore Sigma :
901 - 950 of 8173 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: In vivo expression of OSKM in all reprogrammable mouse strains was induced by continuous administration of doxycycline (Sigma, D9891) in drinking water (1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Cells were plated in 96-well plate in density of 1×106/mL in 200μL of medium and cultured at 37°C and 5% CO2 for 48h in presence of 5μg of lipopolysaccharide (LPS, isolated from E. coli strain 055:B5, Sigma, St ...
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bioRxiv - Microbiology 2022Quote: ... All bacterial strains and libraries were stored at - 80°C for long term storage in 25% sterile glycerol (Sigma). All library assays were performed in NEB10beta strain backgrounds (araD139 Δ(ara-leu)7697 fhuA lacX74 galK (Φ80 Δ(lacZ)M15 ...
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bioRxiv - Genetics 2019Quote: ... Conditional AID degron strains were grown in YPD or YPG and auxin (3-indoleacetic acid; Sigma Aldrich Cat. I3750) at a final concentration of 500µM to deplete AID-tagged proteins ...
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bioRxiv - Plant Biology 2019Quote: ... loading 60 μg protein from SN samples of WT and IM strains onto Amicon ultra centrifugal filters (0.5 mL, 30 kDa MWCO, Millipore) and digesting with 1.2 μg trypsin (sequencing-grade modified ...
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bioRxiv - Immunology 2020Quote: ... ROP5-His6-3xFlag complementation strains were assessed by IFA with a mouse anti-Flag primary antibody (Sigma, clone M2) at 1:500 dilution and Alexa Flour 594 goat anti-mouse IgG secondary antibody (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: Bacterial strains were cultivated in 96-deep-well plates containing 400 μl of 50% TSB (Tryptic Soy Broth, Sigma) with 180 rpm shaking speed for seven days at 25°C and subsequently pooled at equal volume ratios ...
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bioRxiv - Bioengineering 2019Quote: Anabaena flos-aquae (CCAP strain 1403/13F) was cultured for ~2 weeks in Gorham’s media supplemented with BG-11 solution (Sigma) and 10 mM NaHCO3 at 25ºC ...
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bioRxiv - Immunology 2021Quote: ... positive transformants of both strains were grown in 10 mL LB medium (1% tryptone, 0.5% yeast extract, 0.5% NaCl; Sigma Aldrich) supplemented with ampicillin (100 μg mL−1 ...
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bioRxiv - Molecular Biology 2021Quote: Wild-type or mutated pMAL-c2x-malE-L375-his10 plasmids were transformed into Escherichia coli strain BL21 (EMD Millipore) for subsequent growth in LB broth supplemented with 50 μg/ml carbenicillin and 0.2% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The strains and isolates were revived by culturing from frozen stock on YPD agar plates (yeast extract: Sigma Aldrich, #8013-01-2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strains were were cultivated in Lysogeny Broth (Bertani, 1951) medium supplemented with 100 mg/L of ampicillin (Sigma).
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bioRxiv - Genetics 2023Quote: ... The 3ml mix of all three strains was filtered through a 125 ml vacuum filtration system (Sigma-Aldrich #Z290467) fitted with a 0.2μM nylon membrane filters (Sterlitech ...
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bioRxiv - Microbiology 2023Quote: ... The fatty acid methyl esters of the PIA16 strain were obtained from cells grown in tryptic soy broth (Millipore) at 28 °C for 24 h ...
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bioRxiv - Microbiology 2023Quote: ... tb strain H37Rv (ATCC27294 strain) was cultured in Middlebrooks 7H9 broth (Himedia) supplemented with 10% OADC (Himedia) and 0.05% Tween-80 (Sigma) for 4-5 days ...
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bioRxiv - Bioengineering 2022Quote: ... all media components and supplemented chemicals for strain growths and constructions were acquired from Sigma-Aldrich (St. Louis, MO), Thermo Fisher Scientific (Waltham ...
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bioRxiv - Plant Biology 2023Quote: ... Chronic exposure to ROS was implemented by growing the strains in TSB ± 10 µM paraquat (856177-1G, Sigma-Aldrich), a ROS-generating compound ...
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bioRxiv - Microbiology 2023Quote: ... Liquid and solid media for Mtb auxotrophic strains were additionally supplemented with 24 μg/mL L-pantothenate (Millipore Sigma), 50 μg/mL L-leucine (Millipore Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... Once the cultures reached OD 0.1 then 108 CFU/ml of each strain was incubated with 10µM of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma) in 1xPBS (pH 7.2 ...
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bioRxiv - Microbiology 2023Quote: ... Mycobacterial strains were cultured in Difco Middlebrook 7H9 liquid medium (Becton Dickinson, UK) supplemented with 0.2% glycerol (Sigma, UK), 0.05% Tween 80 (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... ΔeutB and the various complemented strains in LMO All primers used in this study were purchased from Sigma-Aldrich and are listed in Table S2 ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitated protein-DNA complexes were captured on protein A-Sepharose beads (Sigma) and eluted in elution buffer (1% SDS and 100 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using Bradford protein assay (Sigma, Missouri, USA). The total protein was then resolved on SDS-PAGE (8-12% ...
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bioRxiv - Immunology 2023Quote: ... and immunoprecipitated with protein A and protein G sepharose beads (Millipore Sigma). After thoroughly washing beads with SLBA buffer (0.15M NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-HA rabbit antibody (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-FLAG mouse antibody (Sigma) ...
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bioRxiv - Plant Biology 2024Quote: ... After determining the protein concentration with Bradford protein assay reagents (Sigma B6916), the protein extract at 5 mg mL-1 was split into two LoBind 1.5 mL tubes and incubated with 100 µM DAPIA or equal volume of DMSO (1% v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Tau protein ladder (Sigma) was used in some experiments as a control ...
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bioRxiv - Biophysics 2022Quote: ... BLUeye protein ladder (Sigma) was used as a standard ...
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bioRxiv - Biophysics 2022Quote: ... standard proteins BSA (Sigma), Apoferritin (Sigma ...
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bioRxiv - Plant Biology 2022Quote: Protein A-sepharose (Sigma) was washed twice with 5 column volumes of buffer HNTG [20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein disulfide isomerase (Sigma) was used in positive control reactions and albumin (Research Organics ...
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bioRxiv - Immunology 2021Quote: ... plus protein A (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... S protein agarose (Millipore) was added to each sample ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Protein standard (Sigma: P5369) was used as standard (ranging from 0-0.5mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... ECL protein detection (Millipore) was performed with a Fusion Fx7 chemiluminescent imager ...
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bioRxiv - Immunology 2024Quote: ... protein G beads (Millipore) were used (2 hours ...