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Citations for Millipore Sigma :
901 - 950 of 7689 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... ½-log serially diluted influenza viruses in virus growth medium plus trypsin (DMEM, 0.1% BSA, 1 µg/mL TPCK-treated trypsin (Sigma, St. Louis, MO, USA)) were added to confluent monolayers of MDCK-SIAT1 cells (37 ...
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bioRxiv - Molecular Biology 2020Quote: ... double mutant KlGal80-K5A/R6Ap (NHKlGal80-K5A/R6Ap or IHGal80-K5A/R6Ap) and KlGal1p (NHGal1p) were expressed in Escherichia coli strain Rosetta (DE3)-pLys from pET-derived (Novagen) expression vectors (pETNHG80 ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysate (1 mg) from various yeast strains was used in immunoprecipitation with anti-Flag M2 magnetic beads (20 μl, Sigma) in a total volume of 1.5 ml of IP-Lysis buffer and incubated with end-over-end rotation for 4 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Each of the five strains were added to a separate well containing 90 μL of 1/10th ISP2 with 1.25% purified agar (Sigma-Aldrich). The surface of each well was imaged for 48 hours using a Nikon Eclipse Ti microscope with a 20x phase contrast lens ...
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bioRxiv - Plant Biology 2020Quote: ... 5mm mycelial blocks of different strains were placed on CM agar with 0.1 mg/mL CFW (Sigma-Aldrich, Shanghai, China), 0.2 mg/mL CR (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: 1dpi and 4dpi transgenic zebrafish larvae infected with fluorescent Mm strains were mounted in 0.8-1% low melting point agarose (Sigma-Aldrich) and imaged on a Leica TCS-SPE confocal on an inverted Leica DMi8 base and imaged using 20x or 40x objective lenses.
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bioRxiv - Immunology 2019Quote: ... Gram-negative strains were lysed with 10% SDS and Proteinase K (20 mg/mL) while Gram-positive strains were lysed with 15 mg/mL lysozyme (Sigma) and 5 mg/mL achromopeptidase (Sigma) ...
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bioRxiv - Plant Biology 2019Quote: ... Pseudomonas syringae strains were grown at 28°C (with shaking at 200 rpm) in King’s B liquid medium (Sigma-Aldrich) containing appropriate antibiotics (King et al. ...
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bioRxiv - Immunology 2021Quote: ... or B6.SJL-PtprcaPepcb/BoyJ mice (B6 CD45.1, JAX strain 2014) by intraarticular injection of 400ug/10uL meBSA (Sigma-Aldrich) antigen into wrist ...
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bioRxiv - Systems Biology 2021Quote: ... coli strain and cultured the cells in M9 minimal medium (Difco) supplemented with 1/2 MEM amino acids solution (SIGMA) and 0.2% (w/v ...
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bioRxiv - Microbiology 2020Quote: All laboratory-adapted strains were maintained in human foreskin fibroblasts (HFF) cells in high glucose DMEM with sodium bicarbonate (Sigma) supplemented with 5% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 2 ml of each recipient and donor strains were combined (three mating pairs in total) and filtered through sterile 0.22 µm membranes (Merck Millipore, GSWP02500), which were incubated over TSA plates for 8 days at 22°C ...
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bioRxiv - Genomics 2021Quote: The high-quality genomic DNA was isolated from all three strains using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... strains were diluted to 0.005 and tested in biological triplicate in serial dilutions of tetracycline (Sigma Aldrich, St. Louis, MO), clarithromycin (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: 10 AB strain wild type embryos per condition per experiment were fixed in either 4% paraformaldehyde (PFA, Sigma Aldrich F8775) in 1X phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain MG1655-seeded NGM plates in the presence or absence of 1 mM auxin (IAA, indole-3-acetic acid, Sigma).
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bioRxiv - Microbiology 2022Quote: ... The strains were maintained on blood agar No2 (Oxoid, Hampshire, UK) supplemented with hemin (5 mg/mL) (Sigma Aldrich, Belgium), menadione (1 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... PCF trypanosomes (Lister strain 427) were cultured in modified SDM-79 medium with 10% heat-inactivated fetal calf serum (Sigma) at 27°C and 5% CO2 (60) ...
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was isolated from fresh bacterial cultures of the strains of interest using the GenElute Bacterial Genomic DNA Kit (SIGMA) according to manufacturers instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples from 24 mice of both strains (n = 3 per group) were separated by SDS-PAGE (8-20%) and transferred onto PVDF membranes (Millipore). Afterward ...
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bioRxiv - Microbiology 2020Quote: ... difficile strains were first grown on reduced Brain Heart Infusion (BHI) agar plates supplemented with 0,5% yeast extract (Sigma-Aldrich) and Clostridium Difficile Selective Supplements (CDSS ...
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bioRxiv - Systems Biology 2019Quote: ... Technical triplicate spectral data was searched against a customized reference proteome comprised by the reference proteome of the respective strain (see above) appended to the UPS2 fasta sequences (Sigma) using Proteome Discoverer 2.1 (Thermo) ...
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bioRxiv - Synthetic Biology 2021Quote: Chromatin fractionation of WT and SpH2A.WAt strains was performed as previously described [82] and fractions were analyzed by western blotting using anti-αtubulin (T6199, Sigma), anti-H3 (ab1791 ...
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bioRxiv - Molecular Biology 2021Quote: ... Obtained complemented strains were grown at 37°C for 16 h in BHI supplemented or not with 1 mM of IPTG (Sigma) until stationary phase was reached ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µL of stationary phase cultures of the complemented strains were spotted in BHI agar plates supplemented with Congo Red (Sigma) 25 µg.mL-1 and IPTG 1 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was first isolated from 40 dpf wild-type AB strain ovaries using TRI reagent (Cat. No. T9424; Sigma-Aldrich) and then cDNAs were synthesized with RETROScript Reverse Transcription Kit (Cat ...
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bioRxiv - Immunology 2020Quote: ... theta strain was suspended in Milli-Q water and mixed with an equal volume of 90% phenol solution (Sigma, 328111). The suspension was stirred continuously and maintained at 68 °C ± 2 °C for 30 min ...
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bioRxiv - Genetics 2020Quote: Paromomycin sensitivity was evaluated at 27°C by following over nine days the growth of four subcultures of wild-type and mutant mat+ strains on M2 medium supplemented (or not) with 500 μg ml-1 of paromomycin (Sigma). For each strain ...
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bioRxiv - Synthetic Biology 2022Quote: ... cerevisiae strains for preparation of competent cells were cultivated in YPD medium consisting of 10 g/L yeast extract (Merck Millipore), 20 g/L peptone (Difco ...
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bioRxiv - Microbiology 2022Quote: ... ljungdahlii strain and its derivatives was carried out in 125 mL serum bottles (Sigma-Aldrich, Merck Life Science, Gillingham, UK) with a working volume of 30 mL ...
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bioRxiv - Neuroscience 2022Quote: ... mice were weighed and received an intraperitoneal (i.p.) injection of sterile saline (SAL) or 5 mg/kg lipopolysaccharide (LPS, strain O111:B4, Sigma-Aldrich) dissolved in sterile saline14,66 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Yeast strains were grown in synthetic dropout (SD) media containing Yeast Nitrogen Base Without Amino Acids mix (Sigma-Aldrich Y0626) supplemented with 10 g/L glucose ...
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bioRxiv - Microbiology 2022Quote: ... strain CNRVC19247 was cultured in alkaline nutrient agar (casein meat peptone E2 from Organotechnie, 20 g; sodium chloride from Sigma, 5 g ...
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cultures were then diluted 1/100 and grown at 370C for 12 hours in 96-well microtiter plates (16 wells for each strain) in the absence or presence of a range of concentrations of trimethoprim (Sigma). 600 nm OD data were collected at 10 min intervals ...
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bioRxiv - Molecular Biology 2023Quote: ... coli MC1061 strain and Luria-Bertani (LB)-agar supplemented with ampicillin (100 μg/mL, Sigma-Aldrich, St. Louis, MO, USA) for selection ...
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bioRxiv - Microbiology 2022Quote: ... the wild-type H99 and isw1Δ mutant strains were either untreated or were treated with 10 μg/ml FLC (Sigma) in 50 ml YPD media at 30°C until cell densities reached the exponential phase (approximately 6 to 7 hours) ...
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bioRxiv - Neuroscience 2023Quote: ... Adult male mice of the NOD-SCID strain (∼30–35 g) were administered 2 µl of 6-hydroxydopamine (6-OHDA, Sigma) dissolved in PBS at a concentration of 3.75 µg/µl with the addition of 0.01% ascorbic acid at the rate of 0.5 µl/min during a stereotaxic surgery ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of the cgs-3xFlag constructs in the resulting Atu strains was confirmed by Western blot using an anti-Flag antibody (Sigma).
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bioRxiv - Plant Biology 2023Quote: ... adjusted to an OD600 of 0.3 for cell death and electrolyte leakage assays and an OD600 of 0.1 for immunoblot assays (final concentration for each strain in a mixture) and were incubated with 100µM acetosyringone (Sigma-Aldrich) for 2-4 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... gondii RHΔKu80Δhxgprt AtTIR1 and RHTatiΔKu80 strains were maintained in Human foreskin fibroblasts (HFF) (ATCC, SCRC-1041) cells in DMEM media (Millipore Sigma). Mycoplasma Detection (MP Biomedicals ...
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bioRxiv - Microbiology 2023Quote: ... overnight P. aeruginosa PA14 strains (Supp. Table 2) were grown under aerobic conditions in Luria-Bertani broth (LB, Millipore Sigma) at 37°C for 16-20 hours at 300 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... Bacteria Escherichia coli and Bacillus subtilis strains were grown in Luria Bertani (LB) medium (Sigma, St. Louis, MO, United States) at 37°C and 180 rpm ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Microbiology 2023Quote: ... 100X dilutions of overnight bacterial cultures of each strain were made in BHI supplemented with 1.25 mM calcium chloride (Sigma, USA) (50 mg L-1 Ca2+ ...
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bioRxiv - Immunology 2023Quote: The Salmonella typhimurium (S. typhimurium ATCC 14028) ATCC 14028 strain was cultured on Tryptic-Soy agar (Sigma-Aldrich, Schnelldorf, Germany) plates overnight to obtain single colonies ...
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bioRxiv - Microbiology 2023Quote: ... elliotti strain 4EA was cultured at 28 °C in 2% proteose peptone (Pronadisa) supplemented with 10 μM FeCl3 (Sigma Aldrich) and 250 μg/ml of both streptomycin sulfate and penicillin G (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The PAO1 ΔoprF+oprF strain containing oprF under an arabinose-inducible promoter was grown in media containing 0.5% L-arabinose (Sigma Aldrich). Unless otherwise noted ...
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bioRxiv - Biochemistry 2023Quote: ... above mentioned yeast strains were grown in the media containing 0.3mM tert-butyl hydroperoxide (t-BOOH) and 1.25mM diamide (Sigma Aldrich, USA) in a serial dilution of 1:10 and incubated for 3 days.