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Citations for Millipore Sigma :
1201 - 1250 of 8173 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ∼10 x 106 LCLs (both LCL#1 and LCL#89) were induced to release virus in complete RPMI 1640 medium containing 20 ng/ml TPA (Sigma-Aldrich Corp. St. Louis, MO, USA) and 3 mM sodium butyrate (Sigma-Aldrich Corp ...
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bioRxiv - Cancer Biology 2023Quote: ... at 1.5 × 105 cells per well and infected with 100-300μl of low-titer virus in the presence of infection mix (1/10 dilution of polybrene [10 mg ml−1 Sigma; 107689-100MG in PBS] in FBS[-]), by centrifuging plates at 1,100 g for 30 min at 37 °C in a Thermo Heraeus Megafuge 40R centrifuge ...
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bioRxiv - Cell Biology 2019Quote: ... or western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-myc from mouse (9E10 ...
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bioRxiv - Cancer Biology 2021Quote: ... antibody overnight and then with equilibrated protein G beads (Millipore Sigma, Protein G Agarose, Fast Flow) for 2h ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein-protein interactions were assessed using the PLA Duolink in situ starter kit (Sigma Aldrich, DUO92101) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using the Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich Cat# BCA1) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Protein-protein interactions were detected using the Detection Kit Red (DUO92008) according to the manufacturer’s (Sigma) instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296) at 4°C for 1 hr for preclearing ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µg of Rab protein were mixed with 5x SYPRO® orange protein gel stain (Sigma) in a volume of 20 µl ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of the collagen was verified by both BCA Protein assay (Sigma-Aldrich, cat: BCA1) and drying via vacuum centrifugation.
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bioRxiv - Cell Biology 2022Quote: ... and the protein concentration of the supernatant assessed by Pierce 660nm protein assay reagent (Sigma, 22660). All samples were diluted to a final 1X Laemmli sample buffer (40% glycerol ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined in the supernatants using Bradford protein assay method (Bradford reagent, Sigma–Aldrich), a standard curve was performed using BSA (bovin serum albumine ...
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bioRxiv - Biophysics 2023Quote: ... The fusion protein was treated with SUMO protease (Sigma-Aldrich SAE0067, 5 U/ mg target protein) to liberate 3CLpro with authentic N- and C-termini (Ser1 and Gln306 ...
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bioRxiv - Microbiology 2024Quote: ... The protein pellet was washed in protein wash buffer (0.3 M guanidine hydrochloride (Sigma Aldrich, USA) in 95% ethanol ...
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bioRxiv - Physiology 2023Quote: ... The total protein content in samples was obtained using a BCA protein assay kit (Sigma-Aldrich) with the SpectraMax Paradigm microplate reader (Molecular Devices ...
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bioRxiv - Biochemistry 2022Quote: ... AviTagged trimeric S and RBD proteins were biotinylated using the Enzymatic Protein Biotinylation Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... 9 mg of protein were mixed with 20 µL of S-protein Agarose beads (#69704, Novagen), that had been previously washed in extraction buffer with 1% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... After determining the protein concentration through a Bradford protein assay using Bradford reagent (Sigma, cat# B6916), each sample was diluted with an appropriate volume of RIPA buffer mixed with 1x Halt protease & phosphatase inhibitor cocktail to achieve a final protein amount of 20 µg protein ...
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bioRxiv - Microbiology 2019Quote: The gene encoding the NP of the KASJH strain (Genbank Accession No. KP663733) was cloned into the pET28a (+) vector (Novagen, Gibbstown, NJ, USA). NP protein was then purified from E ...
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bioRxiv - Immunology 2021Quote: Leishmania donovani strain NLB-065 was obtained from hamster’s spleen by aspirate and cultured in complete Schneider’s Insect media (Sigma-Aldrich Laborchemikalien GmbH, Seelze, Germany). Promastigotes at the stationary phase were harvested by centrifugation at 2,500 rpm at 4°C for 15 minutes as described (11) ...
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bioRxiv - Microbiology 2019Quote: ... DNA was prepared from a 3 ml culture of the representative strain using the GenElute ™ Bacterial Genomic DNA Kit (Sigma Aldrich, Sweden) [7].
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bioRxiv - Evolutionary Biology 2021Quote: ... 2019) or the background strain CEA17) were incubated 4 h at 37°C in Czapek-Dox medium (CZD, Sigma-Aldrich Chemie, Munich, Germany) to induce swelling ...
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bioRxiv - Microbiology 2019Quote: ... pylori strains was assessed by the agar dilution method against a panel of 7 antibiotics purchased from Sigma-Aldrich (St. Louis, MO, USA), including metronidazole (MNZ) ...
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bioRxiv - Microbiology 2019Quote: ... infected with the wAlbB Wolbachia strain were cultured in 75 cm2 culturing flask with 12 ml of Mitsuhashi and Maramorosch medium (M&M) (Sigma Aldrich, NSW, Australia) and Schneider’s medium (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Strains containing AID tag alleles were treated with final concentrations of 250 µM 3-indole acetic acid (Sigma-Aldrich, St. Louis, MO, USA) in DMSO and buffered with 50 mM potassium phosphate buffer at pH 6.2 for the 30 minutes prior to harvesting or imaging ...
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bioRxiv - Cell Biology 2022Quote: ... coli strains XL 1 blue and K-12 MG1655 were cultured overnight in Luria Bertani (LB) broth media (Sigma Aldrich, cat. no: L3022). Overnight cultures were diluted to OD 600 ∼0.5 in RPMI1640 media supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... Both strains were initially grown anaerobically at 37°C for 3–5 days on Bifido Selective Media (BSM) agar plates (Millipore Sigma, Burlington, MA), from which a single colony was selected and grown in BSM broth (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... coli DH10B (remaining plasmids) were used as cloning strains and electroporated with desalted ligation/Hot Fusion reaction (MF-Millipore membrane, VSWP, 0.025 μm).
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bioRxiv - Genetics 2024Quote: ... Each wild strain was exposed to the following benzimidazoles or macrocyclic lactones at the denoted concentrations: abamectin (2 nM) (Millipore sigma, Catalog # 31732), albendazole (10.65 μM ...
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bioRxiv - Microbiology 2024Quote: The recipient strain was grown in LB broth and donor strain was grown in LB broth with 0.2 um filter sterilized 25 µg/mL Uracil (Sigma-Aldrich, St. Louis, MO) supplemented with 30 mg/L gentamicin and both strains were incubated overnight at 30 ℃ and 200 RPM ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA was extracted from groups of 4-5 aphids of the above-described strains using TRI Reagent® (T9424, Sigma-Aldrich, USA) and Direct-Zol RNA extraction Kit (R2052 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein A Sepharose beads (GE17528001; Sigma) blocked with 1mg/mL BSA (10848 ...
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bioRxiv - Neuroscience 2021Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma ...
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bioRxiv - Immunology 2021Quote: ... Magna Beads Protein A+G (Millipore) was blocked with PBS + BSA (5 mg/mL ...
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bioRxiv - Microbiology 2020Quote: ... Protein G Magnetic Beads (EMD Millipore), 30 μl per sample ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were from Sigma-Aldrich (ovalbumin ...
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bioRxiv - Neuroscience 2019Quote: ... using protein sepharose-A resin (SIGMA), prepared by washing in PBS and equilibration buffer (HEPES pH7.4 20mM ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Biophysics 2020Quote: ... previously coated with Protein A (Sigma), blocked in TBS-BSA buffer and subsequently incubated with anti-myc antibody (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... BCA protein Assay Kit (Sigma-Aldrich) was used to quantify proteins in all the fractions and 30 µg of proteins per fraction were separated by SDS-PAGE for immunoblot.
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bioRxiv - Biochemistry 2021Quote: ... bound to Protein-A Sepharose (Sigma), and ubiquitination reactions were performed as described8 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein A-Sepharose beads (Sigma-Aldrich) were used to purify the antibody/protein complex ...
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bioRxiv - Immunology 2022Quote: Magna Beads Protein A+G (Millipore) were blocked with PBS + BSA (5mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... protein-A agarose Fastflow beads (Millipore) were washed twice with IP Buffer and then blocked in IP Buffer containing 2% BSA by rotating overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Magnetic anti-Protein A beads (Sigma) were washed in a Beads Wash Buffer (BWB ...
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bioRxiv - Developmental Biology 2022Quote: ... Sepharose coupled with protein G (Sigma) was blocked with 5% BSA (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... protein A/G agarose beads (Millipore) were added to the chromatin-antibody complex and incubated for one hour at 4°C on a nutator ...
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bioRxiv - Molecular Biology 2019Quote: ... Dynabeads protein-A (or -G, Millipore) were added and incubated for 4 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... protein G agrarose (60 μl, Millipore) was incubated with anti-phosphotyrosine antibodies (12 μg 4G10 (Millipore) ...