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Citations for Millipore Sigma :
951 - 1000 of 7705 citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cultures were then diluted 1/100 and grown at 370C for 12 hours in 96-well microtiter plates (16 wells for each strain) in the absence or presence of a range of concentrations of trimethoprim (Sigma). 600 nm OD data were collected at 10 min intervals ...
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bioRxiv - Molecular Biology 2023Quote: ... coli MC1061 strain and Luria-Bertani (LB)-agar supplemented with ampicillin (100 μg/mL, Sigma-Aldrich, St. Louis, MO, USA) for selection ...
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bioRxiv - Microbiology 2022Quote: ... the wild-type H99 and isw1Δ mutant strains were either untreated or were treated with 10 μg/ml FLC (Sigma) in 50 ml YPD media at 30°C until cell densities reached the exponential phase (approximately 6 to 7 hours) ...
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bioRxiv - Neuroscience 2023Quote: ... Adult male mice of the NOD-SCID strain (∼30–35 g) were administered 2 µl of 6-hydroxydopamine (6-OHDA, Sigma) dissolved in PBS at a concentration of 3.75 µg/µl with the addition of 0.01% ascorbic acid at the rate of 0.5 µl/min during a stereotaxic surgery ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of the cgs-3xFlag constructs in the resulting Atu strains was confirmed by Western blot using an anti-Flag antibody (Sigma).
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bioRxiv - Plant Biology 2023Quote: ... adjusted to an OD600 of 0.3 for cell death and electrolyte leakage assays and an OD600 of 0.1 for immunoblot assays (final concentration for each strain in a mixture) and were incubated with 100µM acetosyringone (Sigma-Aldrich) for 2-4 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... gondii RHΔKu80Δhxgprt AtTIR1 and RHTatiΔKu80 strains were maintained in Human foreskin fibroblasts (HFF) (ATCC, SCRC-1041) cells in DMEM media (Millipore Sigma). Mycoplasma Detection (MP Biomedicals ...
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bioRxiv - Microbiology 2023Quote: ... overnight P. aeruginosa PA14 strains (Supp. Table 2) were grown under aerobic conditions in Luria-Bertani broth (LB, Millipore Sigma) at 37°C for 16-20 hours at 300 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... Bacteria Escherichia coli and Bacillus subtilis strains were grown in Luria Bertani (LB) medium (Sigma, St. Louis, MO, United States) at 37°C and 180 rpm ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Microbiology 2023Quote: ... 100X dilutions of overnight bacterial cultures of each strain were made in BHI supplemented with 1.25 mM calcium chloride (Sigma, USA) (50 mg L-1 Ca2+ ...
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bioRxiv - Immunology 2023Quote: The Salmonella typhimurium (S. typhimurium ATCC 14028) ATCC 14028 strain was cultured on Tryptic-Soy agar (Sigma-Aldrich, Schnelldorf, Germany) plates overnight to obtain single colonies ...
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bioRxiv - Microbiology 2023Quote: ... elliotti strain 4EA was cultured at 28 °C in 2% proteose peptone (Pronadisa) supplemented with 10 μM FeCl3 (Sigma Aldrich) and 250 μg/ml of both streptomycin sulfate and penicillin G (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... The PAO1 ΔoprF+oprF strain containing oprF under an arabinose-inducible promoter was grown in media containing 0.5% L-arabinose (Sigma Aldrich). Unless otherwise noted ...
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bioRxiv - Biochemistry 2023Quote: ... above mentioned yeast strains were grown in the media containing 0.3mM tert-butyl hydroperoxide (t-BOOH) and 1.25mM diamide (Sigma Aldrich, USA) in a serial dilution of 1:10 and incubated for 3 days.
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bioRxiv - Biochemistry 2023Quote: ... The ligated pTrcHisB/PRNP and pTrcHisB/Prnp constructs were used to transform the Escherichia coli host strain BL21(DE3) (Novagen), genotype F′ ompT hsdSB (rB-mB- ...
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bioRxiv - Microbiology 2023Quote: ... The strains and isolates were revived by culturing from frozen stock on Sabouraud dextrose agar (SDA) plates (Millipore, Dramstadt, Germany) and incubated at 35°C for 48h ...
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bioRxiv - Synthetic Biology 2023Quote: ... Escherichia Coli DH5alpha or BL21-DE3 strains were purchased from New England Biolabs (C2987 and C2527, respectively) and were routinely grown in LB media (Sigma) at 37°C with constant shaking ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strains were routinely grown in LB medium and MOPS/MES minimal medium supplemented with 0.01% casamino acids (CAA) (Sigma-Aldrich), 4 g/L glucose ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Microbiology 2024Quote: ... For the purpose of spore production and genetic manipulations AgN23 strain was cultivated on the solid medium Soya Flour Mannitol (SFM) medium (D-Mannitol (Sigma) 20 g/L ...
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bioRxiv - Cell Biology 2023Quote: ... gondii RH ΔKU80Δhxgprt AtTIR1 and RH TatiΔKU80 strains were grown in Human Foreskin Fibroblasts (HFF) (ATCC, SCRC-1041) in DMEM media (Millipore Sigma) supplemented with 10% FBS [56 ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Biochemistry 2021Quote: ... 30 KDa for T-protein and L-protein and 100 KDa for P-protein) were purchased from Millipore (Billerica, MA, USA). Mut Express II Fast Mutagenesis kit V2 was purchased from Vazyme (Jiangsu ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were sonicated and protein concentration was determined using BCA protein assay (Sigma). 30 μg of total proteins were loaded on 12% SDS–PAGE gel and separated by electrophoresis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... a marker of protein oxidation (protein carbonyls; OxyBlot; s7150; EMD Millipore, Billerica, MA), and a marker of mitochondrial biogenesis (PGC-1α ...
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genomics 2019Quote: Duolink PLA kit was used to detect protein-protein interactions (Sigma Aldrich DUO92101). PLA was performed according to manufacturer’s protocol plus additional negative controls ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Immunology 2020Quote: Total proteins isolated from BMDCs were lysed in CytoBuster protein extraction reagent (Novagen). Protein quantification was performed using NanoDrop One Spectrophotometer ...
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bioRxiv - Cell Biology 2022Quote: ... the other stained for proteins with SYPRO Red Protein Gel Stain (Sigma Aldrich). Both dyes were used according to the manufacturers’ protocols.
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bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Microbiology 2023Quote: ... 1000 µg of protein extracts were cleared with agarose protein A beads (Millipore) at 4 ℃ for 1.5 h on a rocking platform ...
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bioRxiv - Microbiology 2023Quote: ... or with slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose). For GFP-p65 and GFP-COMMD2 the samples were immunoprecipitated as per manufacturer’s instructions with the elution was recovered in either 5x with Laemmli buffer 74 and boiled at 98°C (dry bath ...
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bioRxiv - Plant Biology 2023Quote: ... the protein extract was incubated with 30 μL Protein A Agarose beads (Millipore), 10 μL anti-SIZ1 antibody with rotation at 4°C for 4 h.
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bioRxiv - Microbiology 2023Quote: ... The protein was purified using a Protein A agarose gravity column (Millipore Sigma) followed by SEC using a S200 column.
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Molecular Biology 2024Quote: The protein mixtures were filtered using a low protein binding filter (Millipore Sigma) and purified on a SourceQ15 anion exchange column (Cytiva ...
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bioRxiv - Genomics 2024Quote: ... Protein transfer and total protein content was assessed by Ponceau Staining (Sigma Aldrich). Ponceau Stain was washed out with 1x TBST ...
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bioRxiv - Genetics 2024Quote: ... protein concentration was measured by use of a BCA Protein Assay (SIGMA Aldrich) and samples stored at -80°C.
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were seeded at a density of 2.5×106 cells/well in 12-well plates and spin-fected (2000 rpm for 2 hours at 37°C) with increasing concentrations of virus in the presence of 8µg/mL polybrene (Millipore TR-003-G lot#3287963). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... Near-confluent cells were transduced by aspirating the medium and adding 1ml of the respective virus diluted in 1ml of EGM2 with 4mg/ml polybrene (Sigma, Prod. No. H9268-5G). Cells were then left to incubate for 6h at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Wild-type KP4 cells were transduced with concentrated virus and GFP-expressing cells were selected using puromycin selection (1 μg/mL, Sigma-Aldrich, St Louis, USA). GFP-expressing PDOs were created using the same virus as KP4 but with a slightly different transduction protocol ...