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Citations for Millipore Sigma :
801 - 850 of 8192 citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined using Bradford protein assay reagent (Sigma), measuring absorbance at a wavelength of 595 nm on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A and Protein G-Sepharose (Sigma-Aldrich, #P3391, #P3296), IgG sepharose 6 Fast Flow (#GE17-0969-01).
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bioRxiv - Biochemistry 2022Quote: ... and the protein stained using ReadyBlue protein stain (Sigma-Aldrich). Fluorescent DNA bands and protein bands were quantified using the Bio-Rad Image Lab software 6.0.1.
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bioRxiv - Molecular Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added to the mix followed by incubation at room temperature for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... Two days prior to the experiment we streaked the focal strain on LB-agar (Sigma-Aldrich, Buchs, Switzerland) and incubated overnight at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... Cycloheximide tolerance was evaluated by growing strains on SDA supplemented with 0.2% and 0.5% of cycloheximide (Sigma, USA). Tolerance of NaCl was evaluated by the growth of strains on SDA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: All strains were maintained on OP50 E.coli seeded NGM agar supplemented with 50 IU/mL penicillin (Sigma #P4333), 50 µg/ml streptomycin (Sigma #P4333) ...
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bioRxiv - Microbiology 2020Quote: ... H10407 strain of ETEC was grown overnight at 37°C in ETEC medium containing 1% Casamino Acids (Sigma) and 0.15% yeast extract (Fisher Bioreagents ...
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bioRxiv - Microbiology 2022Quote: LPS from pathogenic bacterial strain Escherichia coli O111:B4 was purchased from Sigma Aldrich (Merck KGaA, Darmstadt, Germany) that has been shown to stimulate host innate immunity47 ...
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bioRxiv - Cell Biology 2022Quote: ... Strains with degradation tags were treated with 250 µM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) in DMSO and 50 mM potassium phosphate buffer ...
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bioRxiv - Immunology 2022Quote: ... Both the strains were grown on Middlebrook 7H10 agar slants supplemented with oleic acid-albumin complex (Sigma-Aldrich) maintained at 39°C in a humidified incubator ...
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bioRxiv - Microbiology 2022Quote: The stained Halo-cnp1 strains were imaged in a redox buffer (100 mM MEA (Sigma-Aldrich, #MG600-25G) with an oxygen scavenger system (van de Linde et al. ...
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bioRxiv - Systems Biology 2021Quote: ... cerevisiae strains for AP-MS were grown in YAPD medium (120 mg adenine hemisulfate salt (CAT # A9126, SIGMA), 10 g Bacto yeast extract (CAT # BD 212720) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Mitochondria activity was visualized by growing serial dilutions of the strains on YPD agar containing 0.02% TTC (2,3,5-Triphenyltetrazolium chloride)(Sigma-Aldrich), and incubating cells in minimum media (1% yeast nitrogen base ...
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bioRxiv - Synthetic Biology 2022Quote: The yeast strains were grown in 1 ml SC drop-out media (Sigma Aldrich, St. Louis, MO, USA) with 2% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... The vector was used to transform chemically competent cells of the Rosetta™ strain of Escherichia coli (Novagen) according to the method described by Inoue et al [311] ...
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bioRxiv - Microbiology 2024Quote: ... coli strains used in this study were DH5α (Bethesda Research Laboratories) for cloning and Rosetta 2 (DE3) (Novagen) for protein production ...
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bioRxiv - Microbiology 2023Quote: ... Strains with the PCdrA∷gfp and pMH487 plasmids were grown with 60 μg/mL Gentamycin (Sigma-Aldrich, G1914) for plasmid selection ...
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bioRxiv - Microbiology 2022Quote: All cloning and other standard molecular biology methods were done on strains grown in LB medium (Lennox, Sigma) and LB agar plates (1.5% agar ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial strains were routinely sub-cultured at 25 °C – 28 °C in tryptic soy broth (TSB, Sigma-Aldrich) or Luria-Bertani medium (LB ...
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bioRxiv - Microbiology 2023Quote: ... thetaiotaomicron strains were grown in BHIS broth (43) supplemented with bile extract from bovine and ovine (Sigma, B8381) at 0.5% ...
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bioRxiv - Cell Biology 2022Quote: ... Colonies of spores from wild-type and deletion strain crosses were replica-plated onto G418 sulphate (Sigma Aldrich) containing YES plates to determine the KanMX positivity score ...
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bioRxiv - Molecular Biology 2023Quote: Procyclic promastigotes of Leishmania major strain LV39 (MRHO/SU/59/P) were cultivated in M199 medium (Sigma Aldrich) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Synthetic Biology 2024Quote: ... Escherichia coli strain DH5α was purchased from New England Biolabs (C2987H) and routinely cultured in LB media (Sigma) at 37 C with shaking.
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bioRxiv - Immunology 2023Quote: Human IAV strains were propagated in MDCK cells in the presence of 1 µg/ ml TPCK trypsin (Sigma) and harvested after 72 hours ...
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bioRxiv - Microbiology 2023Quote: A fresh single colony of the sarA-GFP reporter strain grown overnight was suspended in PBS (D8537, Sigma) and mixed well using a vortexer ...
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bioRxiv - Synthetic Biology 2024Quote: ... toruloides strains cultured in the nitrogen-limited medium using the Spectrum Total RNA Kit (Sigma-Aldrich, Oakville, Canada). First-strand cDNA was synthesized with the SuperScript IV first-strand cDNA synthesis kit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Salmonella strains were grown overnight at 37°C for 12 h in LB/0.3 M NaCl (Sigma-Aldrich) with appropriate antibiotics and sub-cultured (1:20 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... hCEcto stem cells were grown on collagen-coated six well-plate to 50% confluence before they were treated with virus solution and Polybrene (1μg/mL) (Sigma-Aldrich, # H9268) overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... elephas RNAs were reverse transcribed with an oligo dT primer using an enhanced avian myeloblastosis virus reverse transcriptase enzyme (Sigma-Aldrich) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... 6×105 cells were infected in 6-well plates with 125uL of high titer virus in standard media with 5ug/mL Polybrene (Sigma-Aldrich). Media was changed after 24 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... The virus was harvested 48 hours post transfection and filtered through a 0.45 μm PES filter (Merck-Millipore, Burlington, Massachusetts, USA). PM-PDX derived cells were cultured in complete EMEM until a density of approximately 60% was reached ...
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bioRxiv - Cancer Biology 2020Quote: ... Infection of P3 and T98G cells was performed by centrifugation of cells with virus at 2225 rpm for 90 minutes at room temperature in the presence of 10 μg/mL polybrene (Sigma-Aldrich). Enrichment selection using puromycin (1 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Infection of P3 and T98G cells was performed by centrifugation of cells with virus at 2225 rpm for 90 minutes at room temperature in the presence of 10 μg/mL polybrene (Sigma-Aldrich). Enrichment selection using puromycin (1 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... Virus particle-containing cell culture supernatants were sterile filtered over 0.22 µM PES syringe filters (Millex-GP, Sigma Millipore, Burlington, MA), mixed 1:1 with fresh growth medium ...
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bioRxiv - Biochemistry 2021Quote: ... Virus particle-containing cell culture supernatants were sterile filtered over 0.22 µM PES syringe filters (Millex-GP, Sigma Millipore, Burlington, MA), mixed 1:1 with fresh growth medium ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 24-48 h with the virus supernatant supplemented with 8 µg ml-1 polybrene (Sigma-Aldrich #H9268) before being subjected to further experimental procedures.
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bioRxiv - Microbiology 2020Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... all transductions were performed in multiple plates with a range of virus volumes and 8 μg mL−1 polybrene (EMD Millipore), but only the pool with the most optimal transduction efficiency was expanded and/or screened.
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bioRxiv - Microbiology 2021Quote: 3 μl of A/PR/8/34 (H1N1) influenza virus mixed with 1 μl of 5x BSA-coated 10 nm colloidal gold (Sigma-Aldrich) were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil) ...
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bioRxiv - Microbiology 2021Quote: A549-ACE2 cells were infected by incubating the virus for 1h with the cells maintained in DMEM supplemented with 1 μg/ml TPCK-trypsin (Sigma #4370285). The viral input was then removed and cells were kept in DMEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2021Quote: Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma−Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... whole-bodies of virus-injected females (DENV and ZIKV-infected) were homogenised with glass beads in Trizol (Sigma-Aldrich, MA, USA) on a Precellys 24 homogeniser (VWR).
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin (Puromycin dihydrochloride ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin ...
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bioRxiv - Cancer Biology 2022Quote: ... P12-ICHIKAWA cells were infected with Lenti-FUW-Luc-mCh-puro virus and selected in liquid culture with puromycin (Sigma-Aldrich) 2.5µg/mL for 7 days following mCherry+ cell sorting.
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bioRxiv - Microbiology 2019Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56 °C ...
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bioRxiv - Cell Biology 2019Quote: ... were added to each well (around 6 × 105 cells per well) along with a no virus control with 8 µg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight (12-16 hours) ...