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Citations for Millipore Sigma :
851 - 900 of 8192 citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... viral transductions were performed overnight in a 6-well dish using 800 µl of standard ESC medium with 200 µl virus-containing supernatant supplemented with 8 µg/ml polybrene (TR-1003; Sigma-Aldrich). The selection of positive clones was performed by supplementing the culture medium with 1µg/ml puromycin for 3 days ...
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56°C ...
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bioRxiv - Genomics 2021Quote: The titer of lentivirus was determined by transducing 500,000 cells with varying amount (0, 0.5, 1.0, 2.0, 4.0 and 8.0 µL) of concentrated virus and polybrene (Millipore, TR-1003-G, 8 µg/mL,). Viral transduction was performed by centrifuging the lentivirus and cell combination at 1000 RCF for 90 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... and 500 μL) were added to each well along with a no virus control with 7 μg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight ...
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bioRxiv - Immunology 2021Quote: ... On the following day the virus-containing medium was collected and filtered using 0.45 µm filter (Millipore Sigma, Cat. no: SLHVM33RS). Fresh HEK293T cells were infected by adding the lentivirus-containing medium to culture medium followed by 1 to 2 day incubation ...
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bioRxiv - Immunology 2020Quote: ... The swabs were placed into 1 ml of Trizol or 2 ml of virus transport medium comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... A 500 μL aliquot was collected every 10−50 s (depending on the virus) and mixed with 5 μL of 5,000 mg L−1 sodium thiosulfate (Sigma-Aldrich, Germany) to instantly quench the residual free chlorine ...
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bioRxiv - Microbiology 2022Quote: KSHV BAC16 virus stock was prepared by inducing lytic cycle in iSLK-BAC16 cells with Doxycycline (1 µg/ml; Sigma-Aldrich) and sodium butyrate (1 mM ...
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bioRxiv - Neuroscience 2022Quote: ... RabiesΔG-mCherry virus (titer of around 110 TU/ml) was mixed at a 2:1 ratio with silk fibroin (Sigma #5154) to make rabies injection solution (Jackman et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with PBS and virus media with 1 μg/ml TPCK treated trypsin (Sigma-Aldrich, USA) was added ...
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bioRxiv - Plant Biology 2024Quote: ... Twelve-day-old quinoa plants were inoculated on their first pair of true leaves with 20 µl of the virus extracts by mechanical damage using silicon carbide (Sigma-Aldrich). Plants were kept in growth chambers ...
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bioRxiv - Cell Biology 2024Quote: ... virus was collected and used to infect NIH3T3 cells with 6 µg/ml polybrene (Sigma, St Louis, MO, USA; #TR-1003) for another 12 h ...
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bioRxiv - Immunology 2024Quote: ... dissociated organoid cells were seeded in virus-containing organoid culture media with 5 μg/ml polybrene (EMD-Millipore TR-1003-G) overnight ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... cell population potentially harboring latent virus was sorted and stimulated with 10 ng/mL Phorbol 12-Myristate 13-Acetate (PMA, Sigma Aldrich) followed by sorting the GFP (+ ...
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bioRxiv - Biophysics 2022Quote: ... cells at a density of 3×10^6 cells/mL at a ratio of 50 mL virus for 800 mL cells and simultaneously stimulated with 3.75 mM valproic acid (VPA; Sigma Cat# P4543). Pellets were harvested from cells by centrifugation at 48-72 hours after infection and snap frozen.
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bioRxiv - Microbiology 2023Quote: ... Neutralization experiments were performed by diluting the appropriate pseudotyped virus with a 4-fold serial dilution of Albumin (Sigma-Aldrich, A8763), alpha-1-antitrypsin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were trypsinized into small cell clusters and incubated with the concentrated virus particles supplemented with 4 μg/mL Polybrene (Sigma Aldrich), for 60 minutes while centrifuging at 600 rpm at RT followed by 4 hr incubation at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... the gradient-purified virus was subjected to buffer exchange using the Pur-A-Lyzer™ Maxi Dialysis Kit (20k MWCO) (Sigma) at 4°C overnight to replace the iodixanol with PBS.
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... reporter virus particle-containing supernatant was passed through a 0.22 µm Millex SteriFlip Vacuum-driven Filtration System (Sigma-Aldrich, Burlington, MA) before aliquoting and storage at −80°C ...
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bioRxiv - Biophysics 2024Quote: ... Wild-type 3T3 cells at 60-80% confluency were incubated for 24 h with 100X virus suspension and 8 µg/ml of Polybrene Transfection Reagent (Millipore Sigma). To maintain stable knockdown ...
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bioRxiv - Neuroscience 2024Quote: ... For the dual-labeling of Cre and PCP4, guinea pig anti-Cre (1:4000, Zuckerman Institute virus core) and rabbit anti-PCP4 (1:300, #HPA005792, Sigma-Aldrich) were used as the primary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... virus supernatants (30 ml) were treated for 1 h at 37°C with 2500 U ultrapure benzonase nuclease (Sigma, E8263-25KU) and subtilisin64 (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... the medium was replaced with medium containing 2X concentrated virus medium and 8 μg/ml polybrene (Millipore Sigma, TR-1003-G). The virus-containing medium was replaced with fresh medium 24 h post transduction.
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bioRxiv - Microbiology 2024Quote: ... The absence of mycoplasma contamination in the virus stocks was confirmed using the LookOut Mycoplasma PCR Detection Kit (Millipore Sigma; #MP0035).
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bioRxiv - Biophysics 2022Quote: ... Protein G (Sigma) was coupled at 10 µg/mL in the presence of 10 mM sodium acetate buffer pH 4.5 at 30 µL/min for 300 seconds until ∼3500-4000 RU was immobilized ...
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bioRxiv - Microbiology 2021Quote: ... Protein G (Sigma) was covalently coupled following activation ...
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bioRxiv - Biophysics 2020Quote: ... Protein G (Sigma) at 10 μg/mL was coupled in the presence of 10mM sodium acetate buffer pH 4.5 at 30 μl/min for 300 seconds in various channels ...
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bioRxiv - Cell Biology 2019Quote: Protein carbonylation was determined by OxyBlot Protein Oxidation Detection Kit (Millipore). Wild-type animals were synchronized to the young adult stage ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A-Sepharose and Protein G-Sepharose were purchased from Sigma-Alderich ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was determined using the bradford protein assay (Sigma-Aldrich). Proteins were separated in 8% SDS-polyacryamide gel (PAGE-SDS ...
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bioRxiv - Plant Biology 2021Quote: ... Protein blots were stained with Ponceau S reversible protein stain (Sigma) to check transfer efficiency and equal protein loading ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentrations were determined using the BCA protein assay (Sigma-Aldrich) using bovine serum albumin as a standard ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Thirty microliters of protein G/protein A agarose beads (EMD Millipore) were washed with lysis buffer (containing protein inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration was determined by a BCA protein assay (Sigma). A total of 50 µg protein extract was mixed with 5× Laemmli loading buffer (50% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 50 μg were resolved on an SDS/PAGE denaturing gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 60 μg were resolved on an SDS/PAGE gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Microbiology 2024Quote: ... then removed the proteins with a 10K MWCO protein concentrator (Millipore) and quantified the resulting HOSCN solution (typically 3.5 to 4 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein quantification was performed with the BCA Protein Assay Kit (Millipore); for each sample ...
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bioRxiv - Cell Biology 2024Quote: Protein quantification using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich) was carried out under manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentivirus of pooled sgRNA phosphatase library titer was estimated by mixing the cells with the serially diluted virus and measuring GFP% on day three post-infection using a Guava® easyCyte™ Flow Cytometer (Merck Millipore). The cells were then infected with the volume of virus estimated to result in a 30–40% infection rate to increase the probability of a single sgRNA introduction event per cell ...
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bioRxiv - Bioengineering 2021Quote: Cells were cultured in the appropriate culture media and infected with S-pseudotyped virus in the presence of polybrene (Sigma; TR-1003) to a final concentration of 5 µg/ml.
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bioRxiv - Immunology 2019Quote: ... duplicate wells of a 12-well plate were seeded with 1 ml/well of the cell suspension (1 × 106 cells/ml) and virus was added in the presence of 6 μg/ml polybrene (Sigma-Aldrich, #107689) (multiplicity of infection of 25) ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Genomics 2021Quote: All cell lines were transduced using virus-containing supernatant in the presence of 8 µg/ml polybrene (Millipore-Sigma, St. Louis MO). HepG2 expressing dCas9-KRAB were derived by transduction with lentivirus harboring SFFV-dCas9-BFP-KRAB ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-containing supernatant was filtered and used to infect WM115 cells in the presence of 1 μg/mL polybrene (Sigma-Aldrich, H9268). This was repeated with fresh viral supernatant 48 hours later ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...