Labshake search
Citations for Millipore Sigma :
1101 - 1150 of 7705 citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2022Quote: ... Bacterial strains were cultured at 20°C (commensal strains) or 28°C (Pto and D36E) at 200 rpm in liquid 50% TSB medium (Sigma-Aldrich, USA).
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... Hydrolysis was carried out by mixing 20 - 100 µl urine of each sample with 40 µl β-Glucuronidase (K12 strain Escherichia coli, Prod. No. BGALS-RO, Sigma-Aldrich) and 900 µl phosphate buffer (composed of 6.0 g NaH2PO4 x H2O + 14.5 g Na2HPO4 x 2 H2O dissolved in 500 ml water ...
-
bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
-
bioRxiv - Microbiology 2019Quote: ... coli cells with a YFP-tagged genomic copy using a previously developed strain (Taniguchi et al., 2011) and grown under chloramphenicol selection (Sigma Aldrich, USA).
-
bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from Legionella strains cultured for 18 h in AYE medium using the GenElute™ Bacterial Genomic DNA Kit (NA2100, Sigma-Aldrich) and used as template for PCR reactions ...
-
bioRxiv - Microbiology 2021Quote: ... gondii localized using antibody against the RH strain tachyzoites (67) and ubiquitin was localized using mouse antibody clone FK2 (04-263; EMD Millipore Corporation). Parasites were enumerated per parasitophorous vacuole (PV ...
-
bioRxiv - Microbiology 2022Quote: ... aeruginosa lab strain PAO1 was routinely grown overnight by streaking from freezer stocks in tryptic soy agar (TSA) (Sigma, St Louis, MO) plates having 15g/L agar ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The strains were precultured on Yeast Nitrogen Base (YNB) medium without amino acids and with 5 g/L ammonium sulfate (Sigma-Aldrich Y0626) and 10 g/L glucose ...
-
bioRxiv - Microbiology 2020Quote: ... and susceptible (all MIC≤ 1mg/L) strains (S1 to S4) were incubated in cation adjusted Mueller Hinton II Broth II (MHB2, Sigma-Aldrich, USA) at 37°C ...
-
bioRxiv - Microbiology 2022Quote: The resulting epiphytic and endophytic strains were stored in saline TSA broth (1.3% NaCl) (Fluka, Buchs, Switzerland) with 20% glycerol (Sigma-Aldrich, Darmstadt, Germany) at - 20 °C before being tested for plant growth-promoting (PGP ...
-
bioRxiv - Evolutionary Biology 2022Quote: Evolution under antibiotic exposure started with a 1:1 ratio of overnight susceptible and resistant strains diluted 1:50 into fresh media supplemented with ampicillin (Millipore cat# 171254) and lysine (as indicated in the text) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... cerevisiae strains were grown in medium containing synthetic complete (SC) media containing 1x Yeast Nitrogen Base Without Amino Acids mix (Sigma-Aldrich Y0626) supplemented with 10 g/L glucose ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Protoplasts of Exiguobacterium Strain-Molly (EM-P) were generated by growing the cells in 7%DSS-TSB with Lysozyme and Penicillin G (Sigma Aldrich, Germany). Transformation to protoplast was confirmed by a change in cell morphology (Carl Zeiss ...
-
bioRxiv - Microbiology 2023Quote: Please see supplementary Table S1 for a list of strains used in this study Surfactin from Bacillus subtilis >98% (CAS: 24730-31-2) (Lot:0000134293) (Source:0000128758) (Sigma-Aldrich, USA) Fengycin from Bacillus subtilis ≥ 90% (CAS ...
-
bioRxiv - Microbiology 2023Quote: ... The ligated fragment was transformed into the first-step constructed mutant strain and selected by using plates containing p-Cl-phe (4 mg/mL, Sigma, United States). Finally ...
-
bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated from overnight cultures of the wild type and mutant strains using GenElute Bacterial Genomic DNA Kits (Sigma-Aldrich, USA). Library preparation and whole genome sequencing were performed by Glasgow Polyomics ...
-
bioRxiv - Microbiology 2023Quote: ... Escherichia coli ST18 strain was grown at 37°C in media supplemented with 50 µg/ml of 5-aminolevulonic acid (5-ALA, Sigma-Aldrich, USA), with or without shaking (120 rpm shaking rate) ...
-
bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
-
bioRxiv - Immunology 2024Quote: ... light chain and the heavy chains of the included HLA types were expressed in bacterial Bl21 (DE3) pLysS strain (Novagen, cat#69451) and purified as inclusion bodies ...
-
bioRxiv - Microbiology 2024Quote: ... virus stocks of the WN-KOUTV isolate and the previously isolated Kenyan WN Lineage 1a strain (AMH005348) were generated from passage 3 in Vero E6 (Ceropithecus aethiops) cells (Sigma Aldrich, France) and used in this assay ...
-
bioRxiv - Microbiology 2024Quote: ... Overnight cultures of the desired bacterial strains were prepared in microtiter plates using 200 µL of TSB supplemented with 1 % (w/v) glucose (Sigma-Aldrich, UK) to facilitate biofilm formation (Croes et al. ...
-
bioRxiv - Microbiology 2024Quote: ... Each strain was grown in liquid mineral medium (LMM) [43] supplemented with 2 g.L -1 of 99% 13C6-glucose (Sigma-Aldrich, Munich, Germany) as a sole carbon source ...
-
bioRxiv - Developmental Biology 2021Quote: ... Even protein loading and successful protein transfer was confirmed using Ponceau S stain (Sigma Aldrich) prior to probing with primary antibodies (Table S2) ...
-
bioRxiv - Cell Biology 2022Quote: ... for total protein loading quantification before proteins were transferred to PVDF membrane (Millipore, Cat. # IPVH00010). The blots were incubated with primary antibodies against SUMO2/3 (Enzo Life Sciences ...
-
bioRxiv - Immunology 2021Quote: ... with protein concentrations normalized to total protein levels in lysate (BCA assay; Sigma-Aldrich®).
-
bioRxiv - Microbiology 2019Quote: ... and the protein level was measured with the Enhanced BCA Protein Assay kit (Sigma-Aldrich). The extracts which contained 25 μg of total proteins were subjected to 10% SDS polyacrylamide gel ...
-
bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was estimated using the Bio-Rad Protein Assay Dye Reagent (Sigma). 10 µl of liver lysate was transferred to 96-well plate and ALT was measured using a microplate-based ALT activity assay kit (Pointe Scientific ...
-
bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...
-
bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using a BCA protein concentration assay kit (Sigma, Shanghai, China, FP0010). Equal amounts of protein (20 μg ...
-
bioRxiv - Cancer Biology 2020Quote: ... the concentration of EV protein was quantified by using a BCA Protein Assay Kit (Novagen). For BMDC stimulation by EVs ...
-
bioRxiv - Cancer Biology 2021Quote: ... the protein-protein interaction was identified using a commercially available PLA kit (Duolink; Sigma-Aldrich) as described before 34 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore) or GFP-Trap®_A beads (Chromotek) ...
-
bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein levels were then quantified using a Coomassie protein assay kit (B8522, Sigma-Aldrich, USA) followed by 12% SDS-PAGE separation (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then normalized to 6 μg protein and the KGDH assay kit (Sigma) protocol was followed.
-
bioRxiv - Cancer Biology 2023Quote: ... The protein-protein interaction was investigated using a commercially available PLA kit (Duolink; Sigma-Aldrich) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined by Bicinchoninic Acid Kit for Protein Determination (BCA1-1KT, Sigma Aldrich) or the Bradford Protein Assay (BioRad) ...
-
bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and protein concentration in the supernatant was measured using the BCA Protein Assay kit (Sigma).
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by SDS-PAGE and biotinylated proteins detected using Streptavidin-HRP (Sigma # RABHRP3) and Supersignal detection kit (Thermofisher Scientific) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The fusion protein was treated with SUMO protease (Sigma SAE0067, 5 U/ mg target protein) to liberate Mpro with authentic N-(Ser1 ...
-
“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Total proteins extraction from hPSCs was performed in ice-cold RIPA protein extraction buffer (Sigma) supplemented with protease inhibitors (Protease inhibitor cocktail set I ...
-
bioRxiv - Immunology 2022Quote: ... The pseudotyped virus-mAb mixture were then overlayed onto target cell lines in the presence of 5 μg/ml polybrene (Sigma Aldrich, catalog no. TR-1003-G) and analyzed 48 h post-infection ...
-
bioRxiv - Cancer Biology 2019Quote: ∼10 x 106 LCLs (both LCL#1 and LCL#89) were induced to release virus in complete RPMI 1640 medium containing 20 ng/ml TPA (Sigma-Aldrich Corp. St. Louis, MO, USA) and 3 mM sodium butyrate (Sigma-Aldrich Corp ...
-
bioRxiv - Cancer Biology 2023Quote: ... at 1.5 × 105 cells per well and infected with 100-300μl of low-titer virus in the presence of infection mix (1/10 dilution of polybrene [10 mg ml−1 Sigma; 107689-100MG in PBS] in FBS[-]), by centrifuging plates at 1,100 g for 30 min at 37 °C in a Thermo Heraeus Megafuge 40R centrifuge ...
-
bioRxiv - Cell Biology 2019Quote: ... or western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-myc from mouse (9E10 ...
-
bioRxiv - Cancer Biology 2021Quote: ... antibody overnight and then with equilibrated protein G beads (Millipore Sigma, Protein G Agarose, Fast Flow) for 2h ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein-protein interactions were assessed using the PLA Duolink in situ starter kit (Sigma Aldrich, DUO92101) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...