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Citations for Millipore Sigma :
901 - 950 of 8192 citations for Zika Virus Envelope Protein Suriname strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μL of each purified virus library were incubated with 100 μL of an endonuclease cocktail consisting of 1000U/mL Turbonuclease (Sigma T4330-50KU) with 1X DNase I reaction buffer (NEB B0303S ...
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bioRxiv - Microbiology 2022Quote: ... we concentrated ∼75 mL of virus to ∼3 mL (25x concentration) using an Amicon Ultra-15 Centrifugal Filter Unit (Millipore Sigma; UFC905008). All viral and lentiviral infections and transductions were done in the presence of 20 μg/mL DEAE-Dextran (Sigma ...
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bioRxiv - Genetics 2024Quote: ... The recipient cells were infected 48 hours post-transfection of HEK 293 cells with the virus supernatant in the presence of 8 μg/ml polybrene according to the manufacturer’s guidelines (Sigma-Aldrich, MO, USA). The cells transduced with the pLVX-Tight-Puro-FoxM1-dNdK were selected in 2 μg/ml puromycin (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... beginning with a virus stock dilution of 1:5 in complete DMEM media as mentioned above supplemented with DEAE-Dextran (Sigma Aldrich #30461) at the final concentration of 40 mg /ml ...
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bioRxiv - Cancer Biology 2023Quote: ... were spin transduced with 10-20μl of the concentrated virus at 37°C at 2200g for 90 minutes with polybrene reagent (8-10μg/ml, Millipore Sigma, Billerica, MA). The cells were washed with PBS and resuspended in fresh RPMI media supplemented with 10% FBS with 1% penicillin and streptomycin 24 hours post-transduction ...
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bioRxiv - Biochemistry 2023Quote: ... C2C12 cells were seeded in a 6 cm dish and 105-106 virus particles were added dropwise to media containing 8 ug/mL Sequabrene (Sigma S2667-1VL) and 25 mM Hepes buffer ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Cell Biology 2023Quote: ... lentiviral expression plasmids were co-transfected with ΔNRF and VSVG (gifts from J. bear) virus packaging plasmids into HEK293T cells using 50 µg/mL polyethylenimine (PEI)-Max (Sigma-Aldrich Chemistry). Two days later ...
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bioRxiv - Immunology 2024Quote: ... Concentrated virus particles solubilized in IMDM were applied to HL60 cells in the presence of 8 μg/ml polybrene (Sigma-Aldrich #H9268). Clones expressing sgRNAs were selected with 2 μg/ml puromycin ...
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bioRxiv - Cell Biology 2024Quote: 3T3-L1 cells were grown in 6-well plates until 50-60% confluency before the medium was replaced by the concentrated virus mixture supplemented with 8 µg/mL polybrene (Sigma-Aldrich, #107689). After overnight incubation the medium was replaced by DMEM supplemented with 10% CS ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were detected by Western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-Gsp1 from rabbit (Makhnevych et al. ...
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bioRxiv - Immunology 2020Quote: ... Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel, Sigma-Aldrich, Israel), following incubation for 30 min at 4°C ...
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bioRxiv - Microbiology 2019Quote: 4 mL of each strain was grown to an OD600 of 0.3 and filtered through 0.22 µm nylon filters (Millipore GNWP04700). The filter was placed upside down in a 60 mm dish containing 1.2 mL pre-chilled quenching solution (40:40:20 Acetonitrile:methanol:H2O + 0.5% formic acid ...
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bioRxiv - Microbiology 2021Quote: ... Gold strain was performed in 800 ml 2xYT o/n at 37°C after induction with 0.5 mM IPTG (Sigma). Cells were harvested by centrifugation ...
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bioRxiv - Biochemistry 2020Quote: ... or catalytically-inactive BT4656S1_11 (in which Ser 77 is intact but prevents formation of formylglycine during expression in E.coli) were expressed in Escherichia coli strain TUNER (Novagen), and cultured to mid-exponential phase in LB supplemented with 50 μg/mL kanamycin at 37 °C and 180 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... The pdr5Δ strain was grown to mid exponential phase prior addition of either 50 μM MG132 (#C2211, Sigma-Aldrich) dissolved in DMSO or an equivalent volume of DMSO ...
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bioRxiv - Plant Biology 2021Quote: The bacterial strains used were pre-grown for 7 d in Tryptic Soy Broth 50 % (TSB 50 %, Sigma-Aldrich), and washed with 10 mM MgCl2 (series of centrifugations and removal of supernatant ...
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bioRxiv - Microbiology 2019Quote: ... encoding for amino acids D35-I221 of Bordetella pertussis strain Tohama I (UniProt_P04977) was cloned with NdeI and BamHI into pET15b (Novagen) allowing expression of an N-terminally HIS-tagged rPtxS1 (MGSSHHHHHHSSGLVPRGSHM-D35-PtxS1-I221) ...
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bioRxiv - Cell Biology 2021Quote: ... by mixing ~1×107 log-phase cells of each strain on a filter membrane (0.8 μm MCE Membrane Filter, MF-Millipore) on top of a YPD plate for 5 hours at 30 °C ...
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bioRxiv - Molecular Biology 2022Quote: In vivo expression of OSKM in all reprogrammable mouse strains was induced by continuous administration of doxycycline (Sigma, D9891) in drinking water (1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Cells were plated in 96-well plate in density of 1×106/mL in 200μL of medium and cultured at 37°C and 5% CO2 for 48h in presence of 5μg of lipopolysaccharide (LPS, isolated from E. coli strain 055:B5, Sigma, St ...
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bioRxiv - Microbiology 2022Quote: ... All bacterial strains and libraries were stored at - 80°C for long term storage in 25% sterile glycerol (Sigma). All library assays were performed in NEB10beta strain backgrounds (araD139 Δ(ara-leu)7697 fhuA lacX74 galK (Φ80 Δ(lacZ)M15 ...
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bioRxiv - Genetics 2019Quote: ... Conditional AID degron strains were grown in YPD or YPG and auxin (3-indoleacetic acid; Sigma Aldrich Cat. I3750) at a final concentration of 500µM to deplete AID-tagged proteins ...
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bioRxiv - Plant Biology 2019Quote: ... loading 60 μg protein from SN samples of WT and IM strains onto Amicon ultra centrifugal filters (0.5 mL, 30 kDa MWCO, Millipore) and digesting with 1.2 μg trypsin (sequencing-grade modified ...
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bioRxiv - Immunology 2020Quote: ... ROP5-His6-3xFlag complementation strains were assessed by IFA with a mouse anti-Flag primary antibody (Sigma, clone M2) at 1:500 dilution and Alexa Flour 594 goat anti-mouse IgG secondary antibody (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: Bacterial strains were cultivated in 96-deep-well plates containing 400 μl of 50% TSB (Tryptic Soy Broth, Sigma) with 180 rpm shaking speed for seven days at 25°C and subsequently pooled at equal volume ratios ...
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bioRxiv - Bioengineering 2019Quote: Anabaena flos-aquae (CCAP strain 1403/13F) was cultured for ~2 weeks in Gorham’s media supplemented with BG-11 solution (Sigma) and 10 mM NaHCO3 at 25ºC ...
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bioRxiv - Immunology 2021Quote: ... positive transformants of both strains were grown in 10 mL LB medium (1% tryptone, 0.5% yeast extract, 0.5% NaCl; Sigma Aldrich) supplemented with ampicillin (100 μg mL−1 ...
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bioRxiv - Molecular Biology 2021Quote: Wild-type or mutated pMAL-c2x-malE-L375-his10 plasmids were transformed into Escherichia coli strain BL21 (EMD Millipore) for subsequent growth in LB broth supplemented with 50 μg/ml carbenicillin and 0.2% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... The strains and isolates were revived by culturing from frozen stock on YPD agar plates (yeast extract: Sigma Aldrich, #8013-01-2 ...
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bioRxiv - Microbiology 2023Quote: ... The fatty acid methyl esters of the PIA16 strain were obtained from cells grown in tryptic soy broth (Millipore) at 28 °C for 24 h ...
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bioRxiv - Microbiology 2023Quote: ... tb strain H37Rv (ATCC27294 strain) was cultured in Middlebrooks 7H9 broth (Himedia) supplemented with 10% OADC (Himedia) and 0.05% Tween-80 (Sigma) for 4-5 days ...
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bioRxiv - Bioengineering 2022Quote: ... all media components and supplemented chemicals for strain growths and constructions were acquired from Sigma-Aldrich (St. Louis, MO), Thermo Fisher Scientific (Waltham ...
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bioRxiv - Plant Biology 2023Quote: ... Chronic exposure to ROS was implemented by growing the strains in TSB ± 10 µM paraquat (856177-1G, Sigma-Aldrich), a ROS-generating compound ...
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bioRxiv - Microbiology 2023Quote: ... Mycobacterial strains were cultured in Difco Middlebrook 7H9 liquid medium (Becton Dickinson, UK) supplemented with 0.2% glycerol (Sigma, UK), 0.05% Tween 80 (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strains were were cultivated in Lysogeny Broth (Bertani, 1951) medium supplemented with 100 mg/L of ampicillin (Sigma).
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bioRxiv - Genetics 2023Quote: ... The 3ml mix of all three strains was filtered through a 125 ml vacuum filtration system (Sigma-Aldrich #Z290467) fitted with a 0.2μM nylon membrane filters (Sterlitech ...
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bioRxiv - Microbiology 2023Quote: ... Once the cultures reached OD 0.1 then 108 CFU/ml of each strain was incubated with 10µM of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma) in 1xPBS (pH 7.2 ...
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bioRxiv - Microbiology 2023Quote: ... Liquid and solid media for Mtb auxotrophic strains were additionally supplemented with 24 μg/mL L-pantothenate (Millipore Sigma), 50 μg/mL L-leucine (Millipore Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... ΔeutB and the various complemented strains in LMO All primers used in this study were purchased from Sigma-Aldrich and are listed in Table S2 ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 100 mg of protein was incubated with the indicated antibodies overnight and then mixed with protein A or protein G-agarose beads (Millipore), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... For pulldown of DNA-protein complex, protein-A Sepharose (G.E healthcare, 17078001) beads were precoated with anti-protein A antibodies (Sigma, P3775) and anti-HA antibodies (Roche ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... following by adding Protein A-Sepharose or protein G-Sepharose beads (Sigma) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were stained using SYPRO Red Protein Gel Stain (Sigma Aldrich) according to the manufacturers’ protocols.