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Citations for Millipore Sigma :
51 - 100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Chemical deglycosylation was achieved with the GlycoProfileTM Kit (Sigma Aldrich, PP0510) using 1 mg total protein ...
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bioRxiv - Plant Biology 2024Quote: ... Transformed cells were plated on Saccharomyces cerevisiae medium with histidine dropout (Sigma). Vector-only controls were transformed into BY4741 ΔPMR1 and By4741 ...
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bioRxiv - Plant Biology 2024Quote: ... The solution was Miracloth-filtered and immunoprecipitation was performed using anti-FLAG gel affinity (Sigma-Aldrich) over night on a rotating wheel at 4°C (8 rpm) ...
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bioRxiv - Neuroscience 2024Quote: ... Clozapine N-oxide (5 mg/kg CNO, Sigma Aldrich, C0832) and doxycycline (50 mg/kg ...
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bioRxiv - Neuroscience 2024Quote: ... protein isolation and immunoprecipitation was as previously described (Mohammed et al., 2016) using 1×107 OL-differentiated NSCs incubated with rabbit anti-OLIG2 (AB9610, Millipore) and using rabbit pre-immune IgG as control ...
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bioRxiv - Cancer Biology 2024Quote: ... with 5 mM EDTA (Sigma-Aldrich) and 2.5% fetal bovine serum (Gibco) ...
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bioRxiv - Plant Biology 2024Quote: ... Stable transformants were selected using 15 mgmL-1 Hygromycin B (Sigma-Aldrich cat. no. H9773).
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bioRxiv - Cell Biology 2024Quote: ... as primary antibody and anti-rabbit-POD (1:10,000; Merck-Millipore, Cat. AP307P) as secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... dissociated in trypsin-EDTA (Sigma-Aldrich) for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml recombinant human EGF (Sigma-Aldrich) and 0.5 μg/ml hydrocortisone ...
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bioRxiv - Cell Biology 2024Quote: ... filtered using 0.45-μm sterile Millex-HV filter units (Millipore Sigma), and supplemented with 8 μg/ml polybrene (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... and supplemented with 8 μg/ml polybrene (Sigma). Cells were infected by replacing their culture medium with the virus-containing medium for 8 h ...
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bioRxiv - Cell Biology 2024Quote: FLAG (1:2000, Sigma-Aldrich F7425)
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted and coverslipped using PVA DABCO (Sigma-Aldrich #10981). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2024Quote: ... in RIPA buffer (R0268, Sigma-Aldrich), both supplemented with phosphatase and protease inhibitors (A32957 and A32955 ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit GluA1 (1:1,000; PC246) was from Millipore. Rabbit PSD95 (1:1,1000 ...
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bioRxiv - Neuroscience 2024Quote: ... 9661S) was used in combination with goat anti-CHAT antibody (1/100; Millipore; Cat. No. MAB144P) to detect apoptotic MNs in cultures obtained from isogenic or ALS-patient derived cells from the CS52 or the CS29 line ...
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bioRxiv - Neuroscience 2024Quote: ... The protein was quantified according to the Coomassie blue method using bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) as a standard (Bradford 1976) ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4 °C with primary antibody (Anti-GPCR CPR7/NPBWR1 antibody, 0.5 µg/ml, #A08247-1, Boster Bio; Actin, 1/1.000, #A-5441, Sigma). Membranes were washed at RT 4x for 15 min in TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Epoxiconazole was diluted in dimethyl sulfoxide (DMSO, CAS 67-68-5) (Sigma-Aldrich, St. Louis, MO, USA) (final concentration of 0.024% DMSO) ...
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bioRxiv - Neuroscience 2024Quote: ... Other reagents for biochemical analyses were obtained from Sigma-Aldrich®.
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bioRxiv - Plant Biology 2024Quote: ... and 1% agar (SIGMA), and that pH was adjusted to 5.7 with KOH ...
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bioRxiv - Physiology 2024Quote: ... [NH3 + NH +]) was determined spectrophotometrically in 25 µL aliquots of flash frozen plasma by enzymatic ammonia assay (Sigma-Aldrich, USA, Catalog number: AA0100). [TAmm] was calculated from the delta absorbance at 340 nm wavelength before and after the addition of the enzyme L-glutamate dehydrogenase ...
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bioRxiv - Physiology 2024Quote: ... and glycerol standard solution (Sigma, G7793). Lipolysis was normalized with protein amount.
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bioRxiv - Physiology 2024Quote: ... Basal and stimulated lipolysis were determined by measuring glycerol content in the media using free glycerol reagent (Sigma, F6428) and glycerol standard solution (Sigma ...
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bioRxiv - Physiology 2024Quote: ... and 5 µM rosiglitazone (Sigma). After 2 days ...
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bioRxiv - Physiology 2024Quote: ... The cells were resuspended and cultured in DMEM/F12 media (Sigma) with 10% FBS ...
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bioRxiv - Physiology 2024Quote: ... The ingWAT Mouse Immortalized Preadipocyte Cell Line (Millipore cat#: SCC211) was induced to differentiate using AdipoLife DfKt-2 Adipogenesis media (LifeLine Cell Technology) ...
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bioRxiv - Physiology 2024Quote: ... kynurenic acid 1 (nonspecific glutamate receptor antagonist, Sigma-Aldrich). Coronal slices from mouse somatosensory cortex (300μm thick ...
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bioRxiv - Physiology 2024Quote: ... where the astrocyte-specific red fluorescent dye sulforhodamine B (10 mg/ml, Sigma) was intraperitoneally injected into awake mice at a dose of 10 μL/g ...
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bioRxiv - Neuroscience 2024Quote: Mice were intraperitoneally (i.p.) injected with 7.5 mg/kg caffeine (#, C0750, Sigma) or saline at an injection volume of 10ml/kg body weight and tested 2 or 24 h later 12,17 ...
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bioRxiv - Neuroscience 2024Quote: ... Amicon Ultra-4 centrifugal filters with 100 kDa molecular weight cutoff (Merck Millipore, Burlington, MA, USA) along with 0.22 μM Nalgene® syringe filter units (sterile ...
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bioRxiv - Neuroscience 2024Quote: ... along with 0.22 μM Nalgene® syringe filter units (sterile, PSE, Sigma-Aldrich, St. Louis, MO, USA) were used to further purify viral particles before being aliquoted and stored at −80 °C.
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bioRxiv - Neuroscience 2024Quote: ... freeze-thaw cycles were implemented to lyse cells and then with benzonase (50 U/mL; Merck Millipore, Burlington, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged for 15 min at 20 000 g and protein concentration was assessed using Bradford assay (Sigma). 50 µg of protein for each sample was transferred in fresh tubes ...
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bioRxiv - Physiology 2024Quote: ... and 0.1 mg/ml protease (P5147, Sigma-Aldrich) for 30 min ...
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bioRxiv - Physiology 2024Quote: ... The culture medium was a 1:1 mixture of DMEM and Ham’s F-12 medium (D 8900, Sigma-Aldrich). For the concentration-response experiments ...
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bioRxiv - Physiology 2024Quote: ... treated with 1 mg/ml collagenase type XI (C 7657, Sigma-Aldrich) and 0.1 mg/ml protease (P5147 ...
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bioRxiv - Physiology 2024Quote: ... dissolved in polyethylene glycol 400 (PEG, Kollisolv 06855, Sigma-Aldrich), while C-1 (33 mg/kg ...
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bioRxiv - Physiology 2024Quote: ... and maintained at 37 °C with 5% CO2 in a 1:1 mixture of DMEM and Ham’s F-12 medium (D 8900, Sigma-Aldrich), supplemented with 50 ng/ml mouse Nerve Growth Factor-7S (NGF ...
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bioRxiv - Physiology 2024Quote: ... supplemented with 50 ng/ml mouse Nerve Growth Factor-7S (NGF, N 0513, Sigma-Aldrich), 10% horse serum and 50 µg/ml gentamicin ...
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bioRxiv - Physiology 2024Quote: ... DRGs were incubated with a mixture of 1.5 mg/ml collagenase type XI (C 7657, Sigma-Aldrich) and 3 mg/ml dispase (Gibco 17105-041 ...
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bioRxiv - Physiology 2024Quote: ... Citrate synthase activity in C2C12 myotubes was measured with a Citrate Synthase Activity Assay Kit (MAK193, Sigma-Aldrich) and following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... which was supplied with deionized water (Milli-Q IQ 7000, Millipore). The metabolite separation was carried at a flow rate of 380 µL/min ...
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bioRxiv - Neuroscience 2024Quote: ... The flow rate was set to 400 µl/min using a binary buffer system consisting of buffer A (10 mM ammonium formate (Sigma), 0.15% [v/v] formic acid (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 200 ml of chilled (4°C) 4% fresh paraformaldehyde (PFA, Sigma, P6148) at 20 ml/min ...
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bioRxiv - Neuroscience 2024Quote: ... and 200 ng/mL of citric acid 2H4 (Sigma), was added ...
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bioRxiv - Neuroscience 2024Quote: ... and Heparin 2.5 µg/mL (Sigma-Aldrich, H3149). Differentiation supplement SVZ and SGZ ...
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bioRxiv - Neuroscience 2024Quote: ... 15N5 ADP and 13C1015N5 AMP (Sigma) and 200 ng/mL of citric acid 2H4 (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 μl of a 10 µM solution of d6-GABA (Sigma-Aldrich) in Milli-Q water ...