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Citations for Millipore Sigma :
401 - 450 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were plated on polylysine-coated coverslips (Sigma-Aldrich) and cultured for at least 24 hours prior to fixation ...
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bioRxiv - Cell Biology 2024Quote: ... Thymidine 2 mM (S phase; Sigma-Aldrich), RO-336 9 μM (G2 phase ...
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bioRxiv - Cell Biology 2024Quote: ... 900 µL CHCl3 and 200 mg/mL of the antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT; Sigma Aldrich). 3 µL of SPLASH LIPIDOMIX Mass Spec Standard (#330707 ...
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bioRxiv - Cell Biology 2024Quote: ... All chemicals were purchased from Sigma-Aldrich, unless otherwise stated ...
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bioRxiv - Cell Biology 2024Quote: ● Bromophenol blue (Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ● Amino acids (Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... the specific proteins were visualized using a chemiluminescence HRP Substrate system (Millipore, Massachusetts, USA) and LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Developmental Biology 2024Quote: ... dsRNA-injected animals were starved for 2 days followed by re-feeding a standard Tribolium medium supplemented with 0.05% (w/w) Bromophenol blue (BPB) sodium salt (Sigma-Aldrich, MO, USA) overnight as in (4 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.2% bovine serum albumin (BSA) (Sigma Aldrich, #A8412) in serum-free BDM for 30 minutes at 37°C to allow cellular uptake of C16 ...
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bioRxiv - Cancer Biology 2024Quote: ... A universal negative control (Sigma Aldrich, #SIC001) and Cy5-labelled negative control (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Seahorse 96XFe tissue culture plates were coated with 0.01% poly-L-ornithine solution (Sigma-Aldrich, #P4957) and incubated at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and Cy5-labelled negative control (Sigma Aldrich, #SIC005) with no known human homologies were included in all experiments to control for non-specific toxicity and validate successful lipofection.
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bioRxiv - Cancer Biology 2024Quote: siRNA silencing was accomplished using forward transfection of pre-designed MISSION siRNAs from Sigma-Aldrich. A 10 nM final concentration of siBCAT1 (SASI_Hs01_00066058) ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 2 mM L-glutamine (Sigma Aldrich, #1294808), 10 mM glucose (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Cell Biology 2024Quote: ... 2 w/v% bis-acrylamide (Sigma-Aldrich) as the crosslinker ...
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bioRxiv - Developmental Biology 2024Quote: ... bacterial colonies over 100× of the CRISPRi oligo library (∼800,000) were collected and grown in SOC medium (Sigma; S1797). Plasmid DNA was isolated using a midi-prep kit (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.05 w/v% ammonium persulphate (Sigma-Aldrich) as the free radical initiator ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1% v/v acrylic acid N-hydroxysuccinimide-acrylamide ester (AA-NHS, Sigma-Aldrich) to facilitate the binding of fluorophores and proteins ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were adhered on 25mm round coverslips (Menzel-Gläser, #1) by coating the coverslip with Concanavalin IV (ConA, 4mg/ml, Sigma-Aldrich C2010-100MG) by incubating the coverslip with ConA for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... The pre-gel mixture comprised 40 w/v% acrylamide (Sigma-Aldrich) as the monomer ...
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bioRxiv - Cell Biology 2024Quote: ... D2A1 cells were injected with a Nanoject microinjector 2 (Drummond) and glass capillaries (25 to 30 µm inner diameter) filled with mineral oil (Sigma). 13.8nL of cell suspension at 100.106 cells per ml were injected in the duct of Cuvier of the embryos under the M205 FA stereomicroscope (Leica) ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Cell Biology 2024Quote: ... Cells were fixed in 10% neutral buffered formalin before being stained with 60% Oil Red O (Sigma-Aldrich) in water for 20 mins at room temperature in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... and 10% foetal bovine serum (FBS) (Sigma-Aldrich; Cell Sera) and 10 µM enzalutamide (Cayman Chemical ...
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bioRxiv - Cancer Biology 2024Quote: ... This was after 2 washes in Buffer A (Sigma DUOlink PLA kit) and then incubated with the PLA kit Ligation solution (1X ligation buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was stained with DAPI (1 mg/ml in water) for 5 min at RT and coverslips were mounted in fluoroshield (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... All cell cultures were supplemented with 10% Fetal Bovine Serum (Sigma). Cultures were incubated at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2024Quote: ... Xenografts were then mounted in Mowiol aqueous mounting medium (Cat. No. 81381 – Sigma) between two coverslips to allow for double side microscope acquisition.
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bioRxiv - Cancer Biology 2024Quote: ... 1 protease inhibitor tablet (COmplete – SIGMA), 1 phosphatase tablet (PhosSTOP – Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with the corresponding MINUS/PLUS PLA probes (Sigma DUOlink PLA kit) for 1 hour at 37°C in a warm foil-covered box ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescent BCG:SSI colonies were selected and grown in 5mL of liquid Middlebrook 7H9 broth supplemented with 10% ADC and Hygromycin 50μg/mL (Cat. No. H7772 – Sigma) in 50mL centrifuge tubes at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Both cell lines were kept and grown in Dulbecco’s modified Eagle medium (DMEM) high glucose (Biowest) and supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich) and antibiotics (100 U mL−1 penicillin and 100 μg mL−1 streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with 0.5 µg/ml Hoechst 33258 in 2x saline sodium citrate (SSC) (Sigma) for 15 min and exposed to UV for 30 min in 2x SSC before being treated with exonuclease III (3 U/µl ...
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bioRxiv - Cancer Biology 2024Quote: Custom Lentiviral shRNA USP50 sequence (based on the USP50-7 siRNA sequence C UAC CCA GCA UUU ACG) or non-targeting control sequence cloned into the pLKO-puro-IPTG-3xLacO vector were made by Sigma-Aldrich (Merck). Flp-InTM HeLa cells were lentivirally infected with NTC or USP50 shRNA as per the manufacturer‘s protocol and then cells selected using Puromycin ...
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bioRxiv - Cancer Biology 2024Quote: ... Following amplification cells were washed for 15 min with wash buffer B (Sigma Duolink PLA kit) and incubated with Hoechst for 5 min before another 15 min wash with buffer B ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.1 mM 8-Bromoadenosine 3’,5’-cyclic monophosphate sodium salt (Sigma, No.B7880) and 0.1 mM 3-Isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Developmental Biology 2024Quote: ... Tamoxifen (Sigma-Adrich; T5648) is suspended in sesame oil at 10mg/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... and 2 μm Dorsomorphin (‘‘DS’’; Sigma, No. P5499). In the first 24 hours following passage ...
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bioRxiv - Developmental Biology 2024Quote: ... then embedded in glass capillary tubes with paired pistons (Sigma Z328510 paired with BR701938, or Sigma Z328502 paired with BR701934) for embryos ...
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bioRxiv - Developmental Biology 2024Quote: ... with protease inhibitors (Sigma; 04693159001) and phosphatase inhibitors (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.1 mM 3-Isobutyl-1-methylxanthine (IBMX; Sigma, No.I5879) (DCI) ...
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bioRxiv - Developmental Biology 2024Quote: ... monothioglycerol (Sigma, No. M6145), Glutamax (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... and 100 nM retinoic acid (RA, Sigma, No. R2625). At day 15 of differentiation ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were washed in MACS buffer (1× PBS, 0.5%BSA, and 2mM EDTA) and blocked with 5% goat serum (Sigma; NS02L) in MACS buffer at 4°C for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... E13.5 or E14.5 hearts were micro-dissected from freshly harvested embryos in PBS with heparin (10 Units/mL) (Sigma #H3393) or incubated in 1X RBC lysis solution (Roche #11814389001 ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse anti-beta actin (1:10000, Sigma; 1978), mouse anti-Cas9 (1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 50 nM dexamethasone(Sigma, No. D4902), 0.1 mM 8-Bromoadenosine 3’,5’-cyclic monophosphate sodium salt (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... and valproic acid (Sigma; P4543) were dissolved in water at stocks concentrations of 100mg/ml and 1M ...
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bioRxiv - Developmental Biology 2024Quote: Sodium butyrate (Sigma; B5887) and valproic acid (Sigma ...