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Citations for Millipore Sigma :
351 - 400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and α-Tubulin mouse antibody (Sigma-Aldrich, T9026). Secondary antibodies used included anti-Mouse IRDye 680RD (LI-COR ...
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bioRxiv - Cancer Biology 2024Quote: ... K562 cells were cultivated in Iscove Modified Dulbecco Media (IMDM) supplemented with 2 mM L-glutamine (Sigma-Aldrich), Nalm6 cells in RPMI-1640 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 ug/mL hydrocortisone (Cat# H0888, Sigma-Alrich), 2% FBS and 1% antibiotic/antimycotic at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Cancer Biology 2024Quote: ... Isopropanol (Pharmco, 231000099) and 3M sodium acetate (Sigma-Aldrich, S2889) pH 5.2 were added to each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... HCECs were maintained in high glucose DMEM supplemented with 5 nM sodium selenite (Cat# S5261, Sigma-Aldrich), 2 ug/mL apo-transferrin (Cat# T1147 ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 mM sodium orthovanadate (Sigma-Aldrich, S6508) and 2X HALT protease and phosphatase inhibitor cocktail (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Tissues were homogenized in cold RIPA lysis buffer (150 mM Tris-HCl, 150 mM NaCl, 0.5% (w/v) sodium deoxycholate (Sigma-Aldrich, D6750), 1% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 mg/mL hydrocortisone (Sigma-Aldrich, H4001), 20 ng/mL EGF (R&D Systems ...
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bioRxiv - Cancer Biology 2024Quote: ... using X-tremeGENE HP DNA transfection reagent (6366546001, Sigma-Aldrich/Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... LoVo cells were grown in Kaighn’s Modification of Ham’s F-12 Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Slices were treated with 0.1% Sudan Black B (Sigma-Aldrich, Saint Louis, MO) in 70% ethanol to quench lipofuscin autofluorescence ...
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bioRxiv - Cancer Biology 2024Quote: ... the slices were incubated in 0.5% PBST containing 10% bovine serum albumin (BSA) (Sigma-Aldrich, A4503) for 5-6 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... HCT116 cells were grown in McCoy’s 5A Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to an Immobilon PVDF membrane (Millipore), blocked in 5% non-fat dry milk followed by incubation in the respective primary antibodies overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells and CCD841 cells were grown in Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Slices for interface culture were placed onto membrane inserts (Millipore) in 6 well plates with 800µL per well of slice culture media of the composition ...
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bioRxiv - Cancer Biology 2024Quote: ... The slices were then dried on glass microscope slides and mounted with anti-fade solution (Sigma-Aldrich, F4680). Slides were stored at room temperature in the dark prior to imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 µg/mL tetracycline (87128, Sigma-Aldrich). To generate a stably transfected cell line for the inducible expression of L22 with a C-terminal FLAG tag ...
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bioRxiv - Cancer Biology 2024Quote: ... Tetraethylammonium chloride (TEA, Sigma-Aldrich, 30mM) was first added to the superfusing ACSF ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were grown in RPMI-1640 Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein detection using either Immobilon Western HRP Substrate (WBKLS0500, Geyer/Millipore) or SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were then resuspended in Laemmli buffer and boiled at 95 °C for 5 min prior to SDS-PAGE and immunoblot analysis using FLAG antibody covalently conjugated to horse radish peroxidase for detection of precipitated L22 (A8592, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... and 100 U/ml penicillin/streptomycin (Sigma-Aldrich). Cells were cultivated at 37°C with 5% CO2 and 95% humidity ...
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bioRxiv - Cancer Biology 2024Quote: ... Ten ng/ml of PMA and 250 ng/ml Ionomycin (both from Sigma-Aldrich) treatments were performed for 18 hours.
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bioRxiv - Cancer Biology 2024Quote: ... All media were supplemented with 10% fetal bovine serum (SIGMA-Aldrich) and 100 U/ml penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by Bradford assay (Sigma, #B6916), after which samples were subjected to SDS-PAGE and electroblotted onto nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... The cortex was then dissected and dissociated using a papain solution (0.5mM of EDTA, 1mM L-Cysteine and 0.4mg/mL of papain (7 units of papain/mL) (Sigma, #76216)) at 37°C in a water bath for 25 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg/ml heparin (Sigma-Aldrich, #H3149), 20 ng/ml EGF (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µg/mL cholesterol (Sigma-Aldrich, C4951) or 5 µg/mL GGPP (Cayman Chemicals ...
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bioRxiv - Cancer Biology 2024Quote: ... 54 µL of 1 mg/mL polyethylenamine (PEI; Sigma-Aldrich, 408727) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... derived from the cortical region of human fetal brain tissue (Millipore, #SCC007) were cultured as previously described74 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1ug/mL Laminin (Sigma, #L2020) in 2mL of Neurobasal media (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... α-tubulin (1:10000, Sigma, #T5168) and Vinculin (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue culture-treated 10-cm dishes were coated with poly-L-lysine (PLL; Sigma-Aldrich, P1274) for 5 minutes and then washed three times with 1X PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Polybrene (Sigma-Aldrich, 107689) was added at a final concentration of 5 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µl of serum-free medium containing 0.5 mg/ml MTT (Sigma, #M5655) was added to each well and incubated for 2 h at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then fixed with 4% formaldehyde solution (Millipore-Sigma, 1004965000) for 1 hour at room temperature and then washed three times with 1X PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then fixed with 4% formaldehyde solution (Millipore-Sigma, 1004965000) for 1 hour at room temperature and then washed three times with 1X PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... with drinking water also supplemented with 23.1 g/L of D-fructose and 18.9g/L D-glucose (Sigma-Aldrich) for 7 ...
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bioRxiv - Cancer Biology 2024Quote: ... whereas MG132 and cycloheximide were purchased from Sigma-Aldrich (St Louis, MO).
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bioRxiv - Cancer Biology 2024Quote: ... while H929 was supplemented with 0.05 mM β-mercaptoethanol (Sigma). 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... then the reactions were incubated in kinase buffer with 20 μM ATPγS (Sigma-Aldrich; A1388) at 30°C for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... These protein samples were resolved on 10% SDS-polyacrylamide gels and transferred to Immobilon-P membranes (Millipore) using the semidry method (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... protease inhibitors and benzonase (Merck/Sigma-Aldrich). Biotinylated proteins were then purified as described previously (6 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then scrapped in cold PBS supplemented with protease inhibitors (Protease inhibitor cocktail set III, EDTA-free, Merck/Sigma-Aldrich) supplemented with glycine and isolated by centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-MAP2 (IF, Sigma, 1:1000), IRDye secondary antibodies (IB ...
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bioRxiv - Cell Biology 2024Quote: ... guinea-pig anti-VGLUT1 (IF, Sigma, 1:5000), mouse anti-MAP2 (IF ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL PDL and 100 to 200 µg/mL laminin (Merck/Sigma-Aldrich #L2020). For biochemical experiments ...
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bioRxiv - Cell Biology 2024Quote: ... CAS#96736-11-7) was used at a final concentration of 100 μg/ml and thiabendazole (TBZ) (Sigma-Aldrich; T8904) at 5-15 μg/ml in YE media where appropriate ...
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bioRxiv - Cell Biology 2024Quote: Monoclonal anti-HA agarose (A2095) and rabbit polyclonal anti-FLAG (F7425) were obtained from Sigma. Rabbit polyclonal anti-HA (sc-805 ...