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Citations for Millipore Sigma :
1 - 50 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... THP-1 cells were stably differentiated into macrophages by phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) at 5 ng/ml over 48h ...
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bioRxiv - Microbiology 2024Quote: ... a commercial primary FLAG antibody (α-FLAG, Millipore cat# F1804), or a commercial primary His antibody (α-His ...
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bioRxiv - Microbiology 2024Quote: ... anti-Ki67 (EMD Millipore, MAB4190), anti-sucrose isomaltase (Santa Cruz Biotechonology ...
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bioRxiv - Microbiology 2024Quote: ... and 25 mM Tris pH 8) and concentrated with centrifugal concentrators (10 kDa MWCO, Millipore Sigma-Aldrich, Burlington, MA, USA) to a final volume of 0.1 mL ...
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bioRxiv - Microbiology 2024Quote: ... cells were harvested and resuspended in 500 μl protoplast buffer (0.5 M sucrose, 10 mM K2PO4, 20 mM MgCl2 and 0.1 mg ml-1 lysozyme (Sigma)) and incubated at 37 °C for 30 min with shaking at 300 rpm ...
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bioRxiv - Microbiology 2024Quote: Induction of sexual conversion in the E5ind line was performed as previously described by adding 10 nM rapamycin (Sigma-Aldrich, R0395) for 1 h to sorbitol-synchronised cultures at the trophozoite stage (20 h post-synchronisation ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM choline and 50 mM N-acetylglucosamine (Sigma-Aldrich, A3286) were added to the cultures ...
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bioRxiv - Microbiology 2024Quote: ... The cultures were then centrifuged for 7 min at 7,000 × g and supernatant collected and filtered through a 3,000 Dalton MWCO Amicon Ultra-4 filter (Millipore). Aliquots were stored at −80 °C.
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... supplemented with 5% Serum Plus (Sigma Aldrich) and 50 ug/ml penicillin/streptomycin (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... Blots were blocked in 5% skim milk (Carnation) in Tris-buffered saline (TBS)/Tween (TBS + 1% Tween 20; Sigma) overnight at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... prewashed anti-FLAG M2 magnetic beads (Sigma) were added to the concentrated His-Trap eluted product and incubated 8 h at 4°C with gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... ERAD inhibitor eeyarestatin I (5-40 µM, Sigma #E1286), clatharin-mediated endocytosis inhibitor chlorpromazine (10-20 µM ...
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bioRxiv - Microbiology 2024Quote: ... autophagy inhibitor bafilomycin A1 (10 µM, Sigma # 19-148), lysosomal protease inhibitors leupeptin (6.7 µM ...
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bioRxiv - Microbiology 2024Quote: ... johnsoniae mariner transposon library were inoculated in 1 ml of 1/10-strength TSB with and without 0.5 g of sterile sand (50-70 mesh particle size; Sigma-Aldrich), and then incubated without shaking at 20°C for 48 h ...
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bioRxiv - Microbiology 2024Quote: ... 300 mM NaCl in dH2O) supplemented with 1X cOmplete™ EDTA-free protease inhibitor cocktail (Sigma), 1 mM Na3VO4 ...
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bioRxiv - Microbiology 2024Quote: ... lysosomal protease inhibitors leupeptin (6.7 µM, Sigma #E18), E64 (10 µM ...
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bioRxiv - Microbiology 2024Quote: ... ATCC TIB-71) were grown in Dulbecco’s modified Eagle’s medium (DMEM, high glucose) (Sigma-Aldrich, cat# R8758-500ML), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... we had to switch to a 1 µm PTFE membrane (Omnipore, Merck Millipore, Darmstadt, Germany). Filters were stored on dry ice or −80 °C until DNA extraction.
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bioRxiv - Microbiology 2024Quote: ... or anti-HA Affinity Gel (Millipore, cat# E6779) at 4 ℃ overnight to facilitate the immunoprecipitation process ...
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bioRxiv - Microbiology 2024Quote: Mucins were purified from commercially available porcine gastric mucins (PGM, Sigma-Aldrich, M2378) as described in the supplementary materials ...
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bioRxiv - Microbiology 2024Quote: ... 20-30 μg of protein for each sample was separated by 8-15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (Millipore, cat# IPVH00010) using Trans-Blot Systems (Bio-rad) ...
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bioRxiv - Microbiology 2024Quote: ... or Cecropin B (Sigma). After 6h incubation ...
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bioRxiv - Microbiology 2024Quote: ... Cells were well washed with 1x PBS to remove permeabilization solution before blocking with 3% w/v BSA (Sigma-Aldrich, Cat # A7906-100G) in 1x PBS overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 100 μM xanthurenic acid (Sigma Aldrich, Cat# D120804-1G), pH 8.2 at 22°C ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed once with 1X TBS followed by incubation with 0.2% Janus Green (Sigma; Cat. no. 201677) w/v for 15 min ...
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bioRxiv - Immunology 2024Quote: ... kappa (R1-2)) and Brefeldin A (5 μg/ml; Sigma-Aldrich) for 16 hours ...
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bioRxiv - Microbiology 2024Quote: ... and media was replaced every 2-3 days with MEME medium (Sigma), supplemented with 2 mM L-Glutamine (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... 1% non-essential amino acids (Sigma), penicillin-streptomycin (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... penicillin-streptomycin (Sigma) and 10% heat inactivated fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... Caco-2 cells were treated with trimethylamine N-oxide (Sigma) at varied concentrations for a total of 48 hours including subsequent assays ...
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bioRxiv - Microbiology 2024Quote: ... serine (Sigma), L-carnitine (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... and trimethylamine N-oxide (TMAO) (Sigma).
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bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... and approximately 105 CFU mixed with human serum from male AB plasma (Sigma-Aldrich, product number H4522) in a total volume of 200 µl ...
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bioRxiv - Microbiology 2024Quote: ... using a Guava easyCyte Flow Cytometer (Merck-Millipore) and analyzed by FlowJo software (FlowJo LLC).
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bioRxiv - Microbiology 2024Quote: ... Long-term freezer stocks of collected parasites were prepared by mixing 100 uL of collected blood with 400 uL Freezing Solution (9:1 Alsever’s solution (Sigma-Aldrich, Cat#A3551):glycerol) ...
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bioRxiv - Microbiology 2024Quote: ... clatharin-mediated endocytosis inhibitor chlorpromazine (10-20 µM, Sigma # C8138), caveolae-mediated endocytosis inhibitor genistein (20-200 µM ...
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bioRxiv - Microbiology 2024Quote: ... Proteasome inhibitor bortezomib (5 nM, Sigma #5043140001), neddylation activating enzyme inhibitor MLN4924 (10 µM ...
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bioRxiv - Microbiology 2024Quote: ... caveolae-mediated endocytosis inhibitor genistein (20-200 µM, Sigma # G6649), autophagy inhibitor bafilomycin A1 (10 µM ...
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bioRxiv - Microbiology 2024Quote: ... whereas the secondary antibody was Anti-rabbit peroxidase diluted 1:5000 in PBST (Sigma, A6154). The blots were developed with Clarity Western ECL Substrate (BioRad) ...
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bioRxiv - Microbiology 2024Quote: ... The antigen-bound magnetic beads were washed five times with lysis buffer and eluted with 2.5 volumes of 3× FLAG peptide (150 ng/μl, Sigma) for 3 h at 4°C with gently shaking ...
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bioRxiv - Microbiology 2024Quote: ... P3HR-1 ZHT/RHT or Akata cells lytic induction was triggered by addition of 400 nM 4-hydroxy tamoxifen (4-HT) (Sigma #68392-35-8) for 24 h or by 15 µg/ml α-human IgG (Agilent #A042301-2 ...
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bioRxiv - Microbiology 2024Quote: ... The product was concentrated and imidazole removed using Amicon Ultrafra-30 concentrators (Millipore). The concentrated product was then immunoprecipitated using anti-Flag co-immunoprecipitation ...
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bioRxiv - Microbiology 2024Quote: ... neddylation activating enzyme inhibitor MLN4924 (10 µM, Sigma # 5054770001), ERAD inhibitor eeyarestatin I (5-40 µM ...
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bioRxiv - Microbiology 2024Quote: Surface colonization was assessed using three substrates: sand (0.5 gm; 50-70 mesh particle size; Sigma-Aldrich), glass (2 gm ...
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bioRxiv - Microbiology 2024Quote: ... E64 (10 µM, Sigma # E3132) or the E1 ubiquitin activating enzyme inhibitor TAK-243 (0.5 ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with purified mucins or glycogen (Sigma-Aldrich, 10901393001) at a final concentration of 0.5% w/v ...
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bioRxiv - Microbiology 2024Quote: ... The remaining lysate was then incubated with either anti-Flag M2 Affinity Gel (Sigma-Aldrich, cat# A2220) or anti-HA Affinity Gel (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... and 1% penicillin-Streptomycin (Sigma).
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bioRxiv - Microbiology 2024Quote: ... washed once with 1x PBS and resuspended in Buffer A (1 M KH2PO4, pH 7.0, BSA 0.01%) and Cytochrome C (Sigma) solution (0.5 mg/mL) ...