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Citations for Millipore Sigma :
1 - 50 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Proteins in the supernatant and pellet were solubilized in 1XLDS sample buffer (Sigma) and analyzed by SDS-PAGE and western blots with anti-CAPS and anti-alpha-tubulin antibodies.
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bioRxiv - Cell Biology 2024Quote: ... transfection of 30pmol/ml Mission siRNA Universal Negative Control #1 (Sigma-Aldrich) was used as a control.
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bioRxiv - Cell Biology 2024Quote: ... purified CAPS-GST protein was mixed with increasing concentrations of microtubules in Ca2+ Binding-Buffer (80 mM PIPEs, 2 mM MgCl2, 50 uM CaCl2, 1 mg/ml BSA, 10 uM leupeptin (Sigma Aldrich L-2884), 1 uM Calpain Inhibitor Peptide (Sigma-Aldrich C-9181;) ...
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bioRxiv - Cell Biology 2024Quote: ... and 2mM L-glutamine) with Dox (Sigma, D9891). To induce PGCLCs from formative ESCs ...
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bioRxiv - Cell Biology 2024Quote: ... 1×105 ESCs were placed per well of a 12-well plate coated with 16.7 μg/mL bovine plasma fibronectin (Sigma-Aldrich, F1141) in N2B27 medium containing 20 ng/mL Activin A (Peprotech ...
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bioRxiv - Pathology 2024Quote: ... and stained with Masson’s trichrome (Sigma-Aldrich; Merck KGaA). An Olympus light microscope ...
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bioRxiv - Pathology 2024Quote: ... Cells were detached using trypsin (Sigma-Aldrich, T4049) and the number of total cells was confirmed using a Cellometer Auto M10 (Nexcelom Bioscience) ...
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bioRxiv - Pathology 2024Quote: ... 60 mg/kg (Sigma-Aldrich; Merk KgA, Germany), that was dissolved in 0.1 mol/l in citrate buffer was administered intraperitoneally (I.P. ...
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated media and flowthrough media were frozen at −80°C.
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bioRxiv - Pathology 2024Quote: ... The total number of live cells was confirmed using a Cellometer Auto M10 (Nexcelom Bioscience) and Trypan Blue (Sigma-Aldrich, T8154). Cultures were negatively immunoselected for hematopoietic cells using CD45 and CD11b microbeads (Miltenyi Biotec ...
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bioRxiv - Pathology 2024Quote: ... Half of the cultures from each mouse were treated with doxycycline hyclate (5 mg/mL, Sigma-Aldrich D5207) for 48 hours and media was collected ...
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bioRxiv - Pathology 2024Quote: ... flasks showing high ratio of stromal cells to monocytes by visual examination on an inverted microscope were treated with trypsin (Sigma Aldrich, T4049) and harvested cells were transferred to T-75 flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... Dissociation of cells was performed with Trypsin (Sigma, T4049) for 2D cultures and TrypLE (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: sgRNAs were ordered as oligonucleotides from Sigma-Aldrich and inserted into the PX330 CRISPR vector (Addgene 42230 ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM of Benzethonium chloride (Sigma, PHR1425) was used as a positive control ...
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bioRxiv - Cancer Biology 2024Quote: ... and mounted with Aquatex (Sigma, 1.08562.0050). Antibodies and corresponding antigen retrieval methods are listed in Table S6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound primary antibodies were incubated with HRP-conjugated secondary antibodies (Amersham) and detected using chemiluminescence (Luminata HRP substrate, Millipore). List of antibodies used is listed in Supplementary Information 1.
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bioRxiv - Plant Biology 2024Quote: ... or anti-flag (F1804, Sigma), as indicated ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% Newborn Calf Serum (Sigma 12023C); 1% Penicillin/Streptomycin (Sigma P0781) ...
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bioRxiv - Cancer Biology 2024Quote: 3T3-L1 cells (70,000 cells/well - 12 well plate) were grown to 100% confluency in preadipocyte Growth media (High glucose DMEM (Sigma D5796); 10% Newborn Calf Serum (Sigma 12023C) ...
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bioRxiv - Cancer Biology 2024Quote: ... differentiation medium with full induction cocktail was added (High Glucose DMEM (Sigma D5796); 10% FBS ...
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bioRxiv - Plant Biology 2024Quote: ... The insoluble pellet was washed five times repeatedly by resuspension in the isolation buffer containing 0.1 g of quartz sand (Sigma-Aldrich) and vortex ...
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bioRxiv - Plant Biology 2024Quote: ... and 0.1% IGEPAL (Sigma). After centrifugation at 13,000 x g for 10 minutes at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... 1x protease inhibitor cocktail (Sigma), and 0.1% IGEPAL (Sigma) ...
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bioRxiv - Plant Biology 2024Quote: ... 2.5 mg l-1 Dicamba (Sigma-Aldrich, www.sigma-aldrich.com) and 3.5 g l-1 Phytagel (Bartlett et al. ...
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bioRxiv - Plant Biology 2024Quote: ... we used monoclonal HRP-conjugated anti-rabbit (A6154, Sigma-Aldrich) antibody ...
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bioRxiv - Plant Biology 2024Quote: ... 1 % protease inhibitor cocktail (Sigma-Aldrich), 2 mM Na2MoO4 ...
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bioRxiv - Plant Biology 2024Quote: ... with Peroxidase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted from each plant using Extraction and Dilution buffers (Sigma-Aldrich, E7526 and D5688 ...
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bioRxiv - Plant Biology 2024Quote: ... specific primers for each T-DNA line (Supplemental Table 2) were designed using SALK site (http://signal.salk.edu/tdnaprimers.2.html) and were ordered from Sigma (https://www.sigmaaldrich.com/GB/en).Ten seeds from each T-DNA mutant line were sown and seedlings were picked and transferred to pots ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in sesame oil (Sigma) at 6 µg/µL.
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bioRxiv - Plant Biology 2024Quote: Chemical deglycosylation was achieved with the GlycoProfileTM Kit (Sigma Aldrich, PP0510) using 1 mg total protein ...
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bioRxiv - Plant Biology 2024Quote: ... Transformed cells were plated on Saccharomyces cerevisiae medium with histidine dropout (Sigma). Vector-only controls were transformed into BY4741 ΔPMR1 and By4741 ...
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bioRxiv - Plant Biology 2024Quote: ... The solution was Miracloth-filtered and immunoprecipitation was performed using anti-FLAG gel affinity (Sigma-Aldrich) over night on a rotating wheel at 4°C (8 rpm) ...
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bioRxiv - Neuroscience 2024Quote: ... Clozapine N-oxide (5 mg/kg CNO, Sigma Aldrich, C0832) and doxycycline (50 mg/kg ...
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bioRxiv - Neuroscience 2024Quote: ... protein isolation and immunoprecipitation was as previously described (Mohammed et al., 2016) using 1×107 OL-differentiated NSCs incubated with rabbit anti-OLIG2 (AB9610, Millipore) and using rabbit pre-immune IgG as control ...
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bioRxiv - Cancer Biology 2024Quote: ... with 5 mM EDTA (Sigma-Aldrich) and 2.5% fetal bovine serum (Gibco) ...
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bioRxiv - Plant Biology 2024Quote: ... Stable transformants were selected using 15 mgmL-1 Hygromycin B (Sigma-Aldrich cat. no. H9773).
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bioRxiv - Cell Biology 2024Quote: ... as primary antibody and anti-rabbit-POD (1:10,000; Merck-Millipore, Cat. AP307P) as secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... dissociated in trypsin-EDTA (Sigma-Aldrich) for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml recombinant human EGF (Sigma-Aldrich) and 0.5 μg/ml hydrocortisone ...
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bioRxiv - Cell Biology 2024Quote: ... filtered using 0.45-μm sterile Millex-HV filter units (Millipore Sigma), and supplemented with 8 μg/ml polybrene (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... and supplemented with 8 μg/ml polybrene (Sigma). Cells were infected by replacing their culture medium with the virus-containing medium for 8 h ...
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bioRxiv - Cell Biology 2024Quote: FLAG (1:2000, Sigma-Aldrich F7425)
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted and coverslipped using PVA DABCO (Sigma-Aldrich #10981). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2024Quote: ... 9661S) was used in combination with goat anti-CHAT antibody (1/100; Millipore; Cat. No. MAB144P) to detect apoptotic MNs in cultures obtained from isogenic or ALS-patient derived cells from the CS52 or the CS29 line ...
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bioRxiv - Neuroscience 2024Quote: ... The protein was quantified according to the Coomassie blue method using bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) as a standard (Bradford 1976) ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4 °C with primary antibody (Anti-GPCR CPR7/NPBWR1 antibody, 0.5 µg/ml, #A08247-1, Boster Bio; Actin, 1/1.000, #A-5441, Sigma). Membranes were washed at RT 4x for 15 min in TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Epoxiconazole was diluted in dimethyl sulfoxide (DMSO, CAS 67-68-5) (Sigma-Aldrich, St. Louis, MO, USA) (final concentration of 0.024% DMSO) ...
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bioRxiv - Neuroscience 2024Quote: ... Other reagents for biochemical analyses were obtained from Sigma-Aldrich®.