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Citations for Millipore Sigma :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Proteins in the supernatant and pellet were solubilized in 1XLDS sample buffer (Sigma) and analyzed by SDS-PAGE and western blots with anti-CAPS and anti-alpha-tubulin antibodies.
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bioRxiv - Cell Biology 2024Quote: ... transfection of 30pmol/ml Mission siRNA Universal Negative Control #1 (Sigma-Aldrich) was used as a control.
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bioRxiv - Cell Biology 2024Quote: ... purified CAPS-GST protein was mixed with increasing concentrations of microtubules in Ca2+ Binding-Buffer (80 mM PIPEs, 2 mM MgCl2, 50 uM CaCl2, 1 mg/ml BSA, 10 uM leupeptin (Sigma Aldrich L-2884), 1 uM Calpain Inhibitor Peptide (Sigma-Aldrich C-9181;) ...
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bioRxiv - Cell Biology 2024Quote: ... and 2mM L-glutamine) with Dox (Sigma, D9891). To induce PGCLCs from formative ESCs ...
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bioRxiv - Cell Biology 2024Quote: ... 1×105 ESCs were placed per well of a 12-well plate coated with 16.7 μg/mL bovine plasma fibronectin (Sigma-Aldrich, F1141) in N2B27 medium containing 20 ng/mL Activin A (Peprotech ...
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bioRxiv - Pathology 2024Quote: ... tamoxifen (Sigma-Aldrich: T5648) was dissolved in 90% corn oil and 10% ethanol ...
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bioRxiv - Pathology 2024Quote: ... 12 to 16-week-old mice were treated with tamoxifen (Sigma, Cat# T5648) by intraperitoneal injection at a dosage of 50 mg/kg/day for 5 days or 20 mg/kg/day for 10 days (5 consecutive days with a 2-day interval in between) ...
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bioRxiv - Pathology 2024Quote: ... Tamoxifen was first dissolved in 5% ethanol and further diluted in sunflower oil (Sigma, Cat# S5007). Parkin knockout mice (B6.129S4-Prkntm1Shn/J ...
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bioRxiv - Pathology 2024Quote: ... and stained with Masson’s trichrome (Sigma-Aldrich; Merck KGaA). An Olympus light microscope ...
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bioRxiv - Pathology 2024Quote: ... The ascending aorta to the bifurcation at the common iliac artery was dissected and digested with a cocktail consisting of 4 U/mL Liberase TM (Sigma-Aldrich: 5401127001), 60 U/mL hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: ... Cells were detached using trypsin (Sigma-Aldrich, T4049) and the number of total cells was confirmed using a Cellometer Auto M10 (Nexcelom Bioscience) ...
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bioRxiv - Pathology 2024Quote: ... 60 mg/kg (Sigma-Aldrich; Merk KgA, Germany), that was dissolved in 0.1 mol/l in citrate buffer was administered intraperitoneally (I.P. ...
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bioRxiv - Pathology 2024Quote: ... mice were intraperitoneally injected with bafilomycin A1 (BFA; 3μmol/kg, Sigma). BFA was dissolved in 50% DMSO ...
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated media and flowthrough media were frozen at −80°C.
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bioRxiv - Pathology 2024Quote: ... The total number of live cells was confirmed using a Cellometer Auto M10 (Nexcelom Bioscience) and Trypan Blue (Sigma-Aldrich, T8154). Cultures were negatively immunoselected for hematopoietic cells using CD45 and CD11b microbeads (Miltenyi Biotec ...
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bioRxiv - Pathology 2024Quote: ... Half of the cultures from each mouse were treated with doxycycline hyclate (5 mg/mL, Sigma-Aldrich D5207) for 48 hours and media was collected ...
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bioRxiv - Pathology 2024Quote: ... 60 U/mL hyaluronidase (Sigma-Aldrich: H3506), and 60 U/mL DNase I (Worthington Biochemical Corporation ...
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bioRxiv - Pathology 2024Quote: ... flasks showing high ratio of stromal cells to monocytes by visual examination on an inverted microscope were treated with trypsin (Sigma Aldrich, T4049) and harvested cells were transferred to T-75 flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... Dissociation of cells was performed with Trypsin (Sigma, T4049) for 2D cultures and TrypLE (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: sgRNAs were ordered as oligonucleotides from Sigma-Aldrich and inserted into the PX330 CRISPR vector (Addgene 42230 ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µM of Benzethonium chloride (Sigma, PHR1425) was used as a positive control ...
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bioRxiv - Cancer Biology 2024Quote: ... and mounted with Aquatex (Sigma, 1.08562.0050). Antibodies and corresponding antigen retrieval methods are listed in Table S6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound primary antibodies were incubated with HRP-conjugated secondary antibodies (Amersham) and detected using chemiluminescence (Luminata HRP substrate, Millipore). List of antibodies used is listed in Supplementary Information 1.
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bioRxiv - Cancer Biology 2024Quote: The fluorescent GTP analog MANT-GTP was purchased from Millipore Sigma. For each reaction ...
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bioRxiv - Plant Biology 2024Quote: ... or anti-flag (F1804, Sigma), as indicated ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% Newborn Calf Serum (Sigma 12023C); 1% Penicillin/Streptomycin (Sigma P0781) ...
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bioRxiv - Cancer Biology 2024Quote: 3T3-L1 cells (70,000 cells/well - 12 well plate) were grown to 100% confluency in preadipocyte Growth media (High glucose DMEM (Sigma D5796); 10% Newborn Calf Serum (Sigma 12023C) ...
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bioRxiv - Cancer Biology 2024Quote: ... differentiation medium with full induction cocktail was added (High Glucose DMEM (Sigma D5796); 10% FBS ...
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bioRxiv - Plant Biology 2024Quote: ... The insoluble pellet was washed five times repeatedly by resuspension in the isolation buffer containing 0.1 g of quartz sand (Sigma-Aldrich) and vortex ...
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bioRxiv - Plant Biology 2024Quote: ... A horseradish peroxidase-coupled secondary antibody was used and revealed with the Immobilon-P kit from Millipore.
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bioRxiv - Plant Biology 2024Quote: ... and 0.1% IGEPAL (Sigma). After centrifugation at 13,000 x g for 10 minutes at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... 1x protease inhibitor cocktail (Sigma), and 0.1% IGEPAL (Sigma) ...
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bioRxiv - Plant Biology 2024Quote: ... 2.5 mg l-1 Dicamba (Sigma-Aldrich, www.sigma-aldrich.com) and 3.5 g l-1 Phytagel (Bartlett et al. ...
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bioRxiv - Plant Biology 2024Quote: ... we used monoclonal HRP-conjugated anti-rabbit (A6154, Sigma-Aldrich) antibody ...
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bioRxiv - Plant Biology 2024Quote: ... 1 % protease inhibitor cocktail (Sigma-Aldrich), 2 mM Na2MoO4 ...
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bioRxiv - Plant Biology 2024Quote: ... with Peroxidase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted from each plant using Extraction and Dilution buffers (Sigma-Aldrich, E7526 and D5688 ...
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bioRxiv - Plant Biology 2024Quote: ... DNA extraction was performed using a Phenol-Chloroform-Isoamyl alcohol mixture (Sigma, Cat# 77617), and the DNA was resuspended in 200 µL of ddH2O ...
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bioRxiv - Plant Biology 2024Quote: ... specific primers for each T-DNA line (Supplemental Table 2) were designed using SALK site (http://signal.salk.edu/tdnaprimers.2.html) and were ordered from Sigma (https://www.sigmaaldrich.com/GB/en).Ten seeds from each T-DNA mutant line were sown and seedlings were picked and transferred to pots ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% protease inhibitor cocktail set III and 1% phosphatase inhibitor cocktail set IV (EMD Millipore Corporation). Cells were scraped ...
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bioRxiv - Cancer Biology 2024Quote: Palbociclib isethionate (S1579, SelleckChem) was resuspended at 50 mg/ml in 50 mM sodium L-lactate (Sigma-Aldrich) pH 4 buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... spheroids were incubated with 0.5 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) for 2-3 h until fully stained ...
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bioRxiv - Cancer Biology 2024Quote: ... All blots were treated with Immobilon® Western Chemiluminescent HRP Substrate (EMD Millipore Corporation, Burlington, MA, USA) and developed after exposure to an autoradiography film (VWR International ...
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bioRxiv - Cancer Biology 2024Quote: Equal amounts of protein per sample were size-separated by electrophoresis on SDS-polyacrylamide gels and electroblotted onto Immobilon®-P polyvinylidene fluoride (PVDF) transfer membranes 0.45 μm pore size (Merck Millipore Ltd., Cork, IRL). After 1 h of blocking at room temperature with 5% skim milk in PBS 0.1% Tween ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μg/ml insulin solution human (Sigma-Aldrich) and 0.5 μg/ml hydrocortisone (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 2 buffer with 25% w/v (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD, Sigma-Aldrich). Treatments were aliquoted into single and combined daily doses and frozen at -80 ºC ...
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bioRxiv - Cancer Biology 2024Quote: ... and subjected to digestion with 1 mg/ml collagenase type IV (Sigma-Aldrich) for 30 min at 37 ºC under aseptic conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... Telaglenestat (S7655, SelleckChem) was dissolved at 50 mg/ml in 10 mM sodium citrate (Sigma-Aldrich) pH 2 buffer with 25% w/v (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in sesame oil (Sigma) at 6 µg/µL.
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bioRxiv - Plant Biology 2024Quote: ... and 0.2% bromophenol blue dye (Sigma, Cat# 62625289). Samples were subsequently boiled for 5 minutes at 105°C ...