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Citations for Millipore Sigma :
301 - 350 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg/ml of DAPI (MilliPore) was added to stain nuclei ...
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bioRxiv - Cancer Biology 2024Quote: The following antibodies were used in this study: mouse monoclonal GAPDH(G8795; Sigma-Aldrich, Shanghai, China), mouse monoclonal STIM1(sc-166840 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained overnight with primary antibodies for tubulin (Sigma T6074, 1:500) and Par3 (Sigma HPA030443 ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibody solutions were supplemented with 2μg/mL of DAPI (Millipore Sigma) for nuclear staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were next stained with 2 μg/ml of DAPI(MilliPore) and coverslips were mounted on glass-slides with DAKO-fluorescent media (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed using propidium iodide (PI, Sigma, #P4864) or Live/Dead Fixable Yellow dye (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... phosphatase inhibitor (Sigma)) ...
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bioRxiv - Cancer Biology 2024Quote: ... LIF (1000 U/ml, Merck Millipore, #ESG1107), 0.1 mM non-essential amino acids (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Senescence was induced throughout treatment with the DNA-damaging agent Doxorubicin (100nM in all cell lines except MDA-MB-231 and H1299 50nM) (Sigma, #D1515) and the CDK4/6 inhibitor Palbociclib (1μM in SK-Mel-103 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cremophor EL was purchased from Millipore. All chemicals were stored as stock solutions in DMSO or ultrapure water.
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bioRxiv - Cancer Biology 2024Quote: ... ammonium tetrathiomolybdate and bovine serum albumin (BSA) were purchased from Sigma-Aldrich. Disulfiram ...
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bioRxiv - Cancer Biology 2024Quote: ... dissolved in 0.1 M phosphate buffer containing 1 mg/mL X-Gal (Melford BioLaboratories, #MB1001) prepared in dimethylformamide (DMF, Sigma, #D4551)) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred onto a PVDF membrane (Millipore), blocked in 5% milk (dry milk diluted in Tris-buffered saline containing 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse anti-γ-Tubulin Antibody clone GTU-88 (1:5000, Sigma, #T6557) over-night ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were washed three times with ice-cold PBS and then were added with 100 μL of ice-cold lysis buffer supplemented by protease and phosphatase inhibitors (Sigma-Aldrich). Lysates were centrifuged at 10,000 xg at 4 °C for 12 min ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were analyzed using MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex - Immunology Multiplex Assay (EMD Millipore Corporation, Billerica, MA, USA). and custom FAS panel on the Luminex MAGPIX ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 mM methylglyoxal (Sigma-Aldrich, St. Louis, MO, USA), and 16.67 mg Pam2CSK4 (InvivoGen ...
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bioRxiv - Cancer Biology 2024Quote: ... synchronized overnight with aphidicolin (20 μg/mL, Sigma), were treated with inhibitors (48 h ...
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bioRxiv - Cancer Biology 2024Quote: ... NCM356 cells (Incell Corporation, LLC, San Antonio, TX, USA) were cultured in high-glucose Dulbecco’s Modifed Eagle Medium (DMEM) (Sigma-Aldrich, Missouri, USA) supplemented with 10% FBS and antibiotics ...
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bioRxiv - Cancer Biology 2024Quote: ... NCM356 cells (Incell Corporation, LLC, San Antonio, TX, USA) were cultured in high-glucose Dulbecco’s Modifed Eagle Medium (DMEM) (Sigma-Aldrich, Missouri, USA) supplemented with 10% FBS and antibiotics ...
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bioRxiv - Cancer Biology 2024Quote: ... K562 cells were cultivated in Iscove Modified Dulbecco Media (IMDM) supplemented with 2 mM L-glutamine (Sigma-Aldrich), Nalm6 cells in RPMI-1640 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Isopropanol (Pharmco, 231000099) and 3M sodium acetate (Sigma-Aldrich, S2889) pH 5.2 were added to each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 mM sodium orthovanadate (Sigma-Aldrich, S6508) and 2X HALT protease and phosphatase inhibitor cocktail (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Tissues were homogenized in cold RIPA lysis buffer (150 mM Tris-HCl, 150 mM NaCl, 0.5% (w/v) sodium deoxycholate (Sigma-Aldrich, D6750), 1% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 mg/mL hydrocortisone (Sigma-Aldrich, H4001), 20 ng/mL EGF (R&D Systems ...
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bioRxiv - Cancer Biology 2024Quote: ... using X-tremeGENE HP DNA transfection reagent (6366546001, Sigma-Aldrich/Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... LoVo cells were grown in Kaighn’s Modification of Ham’s F-12 Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Slices were treated with 0.1% Sudan Black B (Sigma-Aldrich, Saint Louis, MO) in 70% ethanol to quench lipofuscin autofluorescence ...
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bioRxiv - Cancer Biology 2024Quote: ... the slices were incubated in 0.5% PBST containing 10% bovine serum albumin (BSA) (Sigma-Aldrich, A4503) for 5-6 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... HCT116 cells were grown in McCoy’s 5A Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells and CCD841 cells were grown in Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Slices for interface culture were placed onto membrane inserts (Millipore) in 6 well plates with 800µL per well of slice culture media of the composition ...
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bioRxiv - Cancer Biology 2024Quote: ... The slices were then dried on glass microscope slides and mounted with anti-fade solution (Sigma-Aldrich, F4680). Slides were stored at room temperature in the dark prior to imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 µg/mL tetracycline (87128, Sigma-Aldrich). To generate a stably transfected cell line for the inducible expression of L22 with a C-terminal FLAG tag ...
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bioRxiv - Cancer Biology 2024Quote: ... Tetraethylammonium chloride (TEA, Sigma-Aldrich, 30mM) was first added to the superfusing ACSF ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were grown in RPMI-1640 Medium (Sigma) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein detection using either Immobilon Western HRP Substrate (WBKLS0500, Geyer/Millipore) or SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were then resuspended in Laemmli buffer and boiled at 95 °C for 5 min prior to SDS-PAGE and immunoblot analysis using FLAG antibody covalently conjugated to horse radish peroxidase for detection of precipitated L22 (A8592, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... and 100 U/ml penicillin/streptomycin (Sigma-Aldrich). Cells were cultivated at 37°C with 5% CO2 and 95% humidity ...
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bioRxiv - Cancer Biology 2024Quote: ... Ten ng/ml of PMA and 250 ng/ml Ionomycin (both from Sigma-Aldrich) treatments were performed for 18 hours.
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bioRxiv - Cancer Biology 2024Quote: ... All media were supplemented with 10% fetal bovine serum (SIGMA-Aldrich) and 100 U/ml penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by Bradford assay (Sigma, #B6916), after which samples were subjected to SDS-PAGE and electroblotted onto nitrocellulose membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... The cortex was then dissected and dissociated using a papain solution (0.5mM of EDTA, 1mM L-Cysteine and 0.4mg/mL of papain (7 units of papain/mL) (Sigma, #76216)) at 37°C in a water bath for 25 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µg/ml heparin (Sigma-Aldrich, #H3149), 20 ng/ml EGF (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 µg/mL cholesterol (Sigma-Aldrich, C4951) or 5 µg/mL GGPP (Cayman Chemicals ...
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bioRxiv - Cancer Biology 2024Quote: ... 54 µL of 1 mg/mL polyethylenamine (PEI; Sigma-Aldrich, 408727) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... derived from the cortical region of human fetal brain tissue (Millipore, #SCC007) were cultured as previously described74 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1ug/mL Laminin (Sigma, #L2020) in 2mL of Neurobasal media (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... α-tubulin (1:10000, Sigma, #T5168) and Vinculin (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissue culture-treated 10-cm dishes were coated with poly-L-lysine (PLL; Sigma-Aldrich, P1274) for 5 minutes and then washed three times with 1X PBS ...