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Citations for Millipore Sigma :
9901 - 9950 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The 400 μl of protein lysates were incubated with 20 μl of anti-FLAG® M2 affinity gel (Millipore-Sigma) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The 400 μl of protein lysates were incubated with 20 μl of anti-FLAG® M2 affinity gel (Millipore-Sigma) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 30-50 micrograms of the protein were separated on a 15% polyacrylamide gel and electrophoretically transferred to PVDF membranes (Millipore). Membranes incubated with the primary antibodies against claudin-5 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Biochemistry 2021Quote: ... Insoluble material was removed by centrifugation at 149,000 ×g for 45 min and the solubilized protein was loaded onto a gravity column of 2 mL M2 anti-FLAG affinity matrix (Sigma). The column was washed with 10 mL of wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: The fractions containing eluted proteins were concentrated to ∼5 ml by AmiconTM ultrafiltration devices (10 kDa or 30 kDa molecular weight cut-off) (Millipore) and centrifuged in a bench centrifuge for 10 min at maximum speed to remove any precipitated material before being subjected to size-exclusion chromatography (SEC) ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was assessed 72 hours post transfection via Western Blot using a monoclonal antibody against the FLAG epitope (Sigma) (Figure 5A ...
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bioRxiv - Microbiology 2020Quote: ... the same samples were incubated for 2.5 h at 37°C with protein synthesis inhibitor cycloheximide (10 μg/ml) (CHX; Sigma-Aldrich), of which experimental condition was optimized in our previous reports 18,21 ...
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bioRxiv - Microbiology 2020Quote: ... Loaded proteins were washed with 300 μL Binding Buffer (90% methanol and 10% triethylammonium bicarbonate buffer (TEAB, Sigma-Aldrich, #T7408), adjusted to pH 7.1 using phosphoric acid ...
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bioRxiv - Microbiology 2020Quote: Fractions containing the DnaA-6xHis protein were pooled together and applied to Amicon Ultra Centrifugal Filters with 30 kDa cutoff (Millipore). Buffer was exchanged to Buffer A (25 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... Total protein samples (30 μg) were resolved in 12%-SDS-PAGE and transferred to ImmobilonTM PVDF membranes (Millipore, Burlington, MA) in a semi-dry electroblotting apparatus (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... 20-30μg of protein extract was separated on a 4-20% precast polyacrylamide gel and transferred to a PVDF membrane (Millipore). Membranes were blocked with 5% nonfat milk in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... About 1 mL of lysate with similar protein concentration was added to tubes with 50 μL of anti-FLAG magnetic beads (Sigma) and rotated for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Residual methylation reagents were neutralised by addition of 1M Tris-HCl pH 7.5 to a final concentration of 50 mM Tris-HCl and the protein complex was concentrated to 2 mg mL−1 using 30 kDa cut-off concentrators (Millipore) before injection into a S75 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by 12% SDS–PAGE gels and detected using immunoblotting with a monoclonal α-Flag antibody (Sigma-Aldrich) or a α-GFP antibody (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... After incubation for 90 min at RT the microplates were washed 4X (PBS-Tween 20) and 25 μl of protein LA-HRP diluted 1:3000 (Sigma) was added and the microplates were then incubated for 1h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Eight micrograms of protein per lane were separated by 12% SDS polyacrylamide gel electrophoresis and blotted onto Immobilon-P nylon membranes (Millipore). After transfer ...
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bioRxiv - Microbiology 2021Quote: ... The protein interlayer solution in urea was then incubated at 60° C after adding dithiothreitol (Sigma Aldrich, St. Louis, MO) at 5 mM concentration for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... lysates of tissues or tells were incubated with antibodies overnight (4°C) and subsequently with protein G-agarose beads (Millipore) for 5h (4 °C) ...
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bioRxiv - Microbiology 2021Quote: ... 5 µg of purified MurA protein were mixed with 10 mM uridine 5′-diphospho-N-acetylglucosamine (UDP-GlcNAc, Sigma-Aldrich) in a reaction buffer containing 100 mM Tris-HCl pH8.0 and 150 mM NaCl (final volume ...
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bioRxiv - Plant Biology 2021Quote: ... the total protein was extracted from tobacco expressing CmTPL1-2-HA and CmSVP-FLAG using anti-HA affinity gel (E6779, Sigma), followed by western blotting with anti-FlAG (A8592 ...
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bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
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bioRxiv - Systems Biology 2020Quote: ... TMT labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (Sigma) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The analyzed proteins contained an SHH-tag (Sarkari et al., 2014) and were detected using anti-HA (Sigma-Aldrich, USA) antibodies and anti-mouse IgG-HRP (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Elution of proteins was done by adding 100 µl of binding buffer supplemented with 20 mM IP6-sodium salt (Sigma) and then incubating overnight at 4°C under constant mixing ...
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bioRxiv - Microbiology 2020Quote: ... the bound proteins were eluted with 400 μL of wash buffer supplemented with 250 μg/mL HA peptide (Sigma-Aldrich) by incubation at room temperature for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of 20 to 30 μg of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... and total protein concentration was determined using the Bradford Assay according to the manufacturer’s instructions (Sigma-Aldrich, St Louis, MO). Immunoprecipitations were carried out by incubating cell lysates with antibodies as indicated for 12 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... protein of each sample was separated with 12% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (Millipore). After blocked by 5% fat-free milk (Sangon Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... antibody-bound fraction was immunoprecipitated for 90 minutes at 4°C using 25 µL slurry salmon sperm protein A agarose beads (Millipore). Unbound proteins were removed by washing the beads 5 times in wash buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Cell Biology 2020Quote: ... treated cells were washed with PBS and collected into RIPA buffer (Fujifilm Wako Pure Chemical) containing protein inhibitor (Sigma-Aldrich). The cells were incubated on ice for 30 min and then homogenized ...
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bioRxiv - Cell Biology 2020Quote: ... the codon optimized gene encoding for rPMDs was subcloned into PET28a (+) and protein expression in E.coli cells was induced with 1 mM isopropyl-β-D-thiogalactoside (IPTG) (Sigma). The his-tagged proteins were purified using Ni-NTA chromatography and the concentration was determined using the BCA estimation kit (Pierce).
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bioRxiv - Biophysics 2021Quote: 5−10 μg of proteins were separated on 10% SDS-PAGE and were consecutively electro blotted onto 0.45 µm nitrocellulose membrane (Millipore, USA). The membrane was blocked with 5% non-fat skim milk in PBS-0.1% Tween 20 for 2 hours at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... For ADH/DMTMM reactions, protein samples were incubated with 57mM ADH (d0/d8, Creative Molecules) and 36mM DMTMM (Sigma-Aldrich) for 15 minutes at 37°C while shaking at 350 rpm ...
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bioRxiv - Biophysics 2021Quote: All proteins were concentrated using ultrafiltration concentrators with either 3 kDa or 30 kDa MWCO regenerated cellulose membrane (EMD Millipore), flash frozen in small aliquots in liquid nitrogen ...
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bioRxiv - Biophysics 2020Quote: Wild-type or Y473E mutant human PPARγ LBD (UniProt P37231; residues 203–477, isoform 1 numbering) protein was expressed from a pET46 Ek/LIC vector (Novagen) as a Tobacco Etch Virus (TEV)-cleavable N-terminal Hexa(6x)His-tagged fusion protein in Escherichia coli BL21(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... and the dried protein pellet resuspended in digestion buffer (100 mM Tris-HCl pH 8.5, 1% sodium deoxycholate (Sigma-Aldrich), 5 mM TCEP ...
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bioRxiv - Cell Biology 2021Quote: Equal amount of protein lysates was loaded and resolved on SDS-PAGE gel and transferred onto an PVDF membrane (Millipore). Blots were blocked with 5% BSA for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... + 10 mM dithiothreitol to reduce the proteins and then for 30 min at room temperature with 50 mM NH4HCO3 + 50 mM Iodoacetamide (SIGMA) in the dark to alkylate them ...
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bioRxiv - Cancer Biology 2021Quote: ... Methanol/chloroform extraction was performed on 100ug of protein for each lysate and pellet finally re-dissolved in 100ul of 6M Urea (Sigma), 2M thiourea (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine the concentration of harvested cell lysates and 500 μg of protein lysate was loaded into the supplied spin column (Catch and Release Immunoprecipitation Kit; Millipore). Immunoprecipitation using antibodies against FLAG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was resuspended in digest buffer (0.1 M triethylammonium bicarbonate, pH 8.5, Sigma-Aldrich, tandem mass tag (TMT) labeling using a 6-plex TMT kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested DNA was then incubated with 50 µl of protein A/G agarose beads and 5 µl anti-S9.6 (Millipore, MABE1095) overnight at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... Some samples that were used to obtain or confirm resonance assignments were assembled by mixing protein with equal volume of 1.5 M ammonium sulfate (Sigma-Aldrich), 0.1 M Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10 μg of proteins were separated by electrophoresis in an 10% SDS polyacrylamide gel and transferred onto a polyvinylidene fluoride membrane (PVDF, Millipore). The membranes were blocked with 5% (v/v ...
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bioRxiv - Biophysics 2022Quote: ... The purified protein was concentrated up to 5 mg/mL using Centricon YM-10 and Microcon YM-10 concentrators (Amicon, Millipore). Crystals used in this study were obtained under oxygen exclusion in hanging drops from 0.5 M Hepes pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was then further purified by concentrating and running over a gel filtration column (CYZ superdex 200 increase, Sigma) in 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were washed three times with 0.5 ml of FLAG wash buffer and the bound protein was eluted with 1X SDS-PAGE loading buffer supplemented with 100 µg·ml-1 3X FLAG peptide (Sigma-Aldrich) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... PKG Iβ (71-686) protein was concentrated to 20 mg ml-1 by using 30 kDa cutoff Amicon Ultra (Millipore) and initial crystal screening was performed by Mosquito Crystal robot (TTP Labtech ...