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Citations for Millipore Sigma :
9751 - 9800 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The gel was first washed with water and then incubated with c.a 10 mL of InstantBlueTM Ultrafast Protein Stain (Sigma) for at least 1 hour.
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bioRxiv - Cancer Biology 2023Quote: ... was purified as described (Noland et al., 2016) with the exception that the proteins were treated with 2.5% hydroxylamine (Sigma) at pH 7.0 for one hour at room temperature prior to size exclusion chromatography ...
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bioRxiv - Cancer Biology 2023Quote: ... equal amounts of protein per sample were subjected to SDS–PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore). For immunoprecipitation of FLAG-tagged proteins ...
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bioRxiv - Synthetic Biology 2023Quote: ... The quality of protein transfer was visualized by staining with Ponceau S (0.1% dye in 5% acetic acid, Sigma) and then blocked with 5% nonfat instant milk (Carnation ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Biochemistry 2023Quote: ... proteins were imaged using Immobilon Classico Western HRP substrate or Immobilon Ecl Ultra Western HRP substrate (Millipore, MA, USA) on the iBright FL1500 Imaging System (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... 4µg of the appropriate antibody and 20µl of Magnetic protein A/G beads (Magnachip, EMD-Millipore, Billerica, MA, USA) were used for the IP step ...
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bioRxiv - Molecular Biology 2023Quote: DRIP-WB assay was performed using Magna Nuclear RIPTM (Native) RNA-Binding Protein Immunoprecipitation Kit (Millipore/Sigma # 17-10522) following the manufacturer’s recommended procedure with modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was eluted with 4 CVs of imidazole-free wash buffer supplemented with 150 µg/mL 3xFLAG peptide (Sigma). This was analysed by SDS-PAGE before being concentrated ...
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bioRxiv - Microbiology 2023Quote: For top-down EF-P measurements the purified proteins were desalted on the ZipTip with C4 resin (Millipore, ZTC04S096) and eluted with 50 % (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein samples were run on EZ Protein any KD PAGE (LIFE-iLAB) and then transferred to 0.22um PVDF membranes (Millipore). Membranes were blocked using 5% non-fat milk in PBS-Tween20 0.1% for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed protein solution was then concentrated using Amicon Ultra-4 Centrifugal Filter Units 30 K (Millipore, MA #UFC803024) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The following commercially available antibodies were used to detect targeted proteins: anti-RBM17 (1:1500 dilution; HPA037478, Sigma-Aldrich), anti-SF3B1 (1:3000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess reagents were removed by MeOH/CHCl3 protein precipitation or concentration-dilution using a 0.5 mL Centrifugal Filter (3K, Millipore). Pellets were washed by 500 μL MeOH/H2O (9:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 30–50 μg of protein was fractionated by 10% SDS–PAGE and blotted onto an Immobilon PVDF membrane (Millipore). Non-specific binding sites were blocked by incubation with 5% BSA in Tris-buffered saline (TBS ...
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bioRxiv - Biophysics 2023Quote: ... Lysis was done either by resuspending the cell pellet in 0.8mL of the detergent-based BugBuster Protein Extraction Reagent (Millipore) and agitating for 20min on a rotator ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein complex was eluted with 500 μL elution buffer (0.4 mg/ml 3X Flag peptide (Millipore Sigma F4799) in wash buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were buffer exchanged into 1x PBS (pH8.0) using Amicon Ultra-15 Centrifugal Filter Unit (10 kDa) (Millipore) and flash-frozen.
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bioRxiv - Microbiology 2024Quote: ... Proteins were concentrated by filtration of the eluate in 15-mL Amicon® Ultra 50k filters (Millipore, Burlington, MA). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: Equal amounts of proteins from total cell lysates were resolved by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The blots were blocked with 5% w/v BSA and 0.05% NaN3 in TBST and incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Biophysics 2024Quote: ... Each protein was dialyzed and concentrated against 100LmM ammonium acetate (pH 7.4) using an Amicon 0.5-mL microconcentrator (EMD Millipore) with a 10-kDa MW cutoff and stored at –80L°C until used ...
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bioRxiv - Biophysics 2024Quote: ... The target protein was eluted by application of 5 CVs of buffer E containing 2.5mM d -desthiobiotin (Sigma-Aldrich). Elutions were pooled ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection of specific protein bands was accomplished using a rabbit monoclonal anti-GLRX3 antibody (1:1,000, HPA028941, Sigma-Aldrich), ferritin (1:1,000 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mg mL-1 catalase from bovine liver (lyophilized powder, 2000-5000 units/mg protein, Sigma-Aldrich, Missouri, US) and 50 mM SPB (pH 7.2 ...
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins were transferred from the gel to Immobilon®-P PVDF membranes (Millipore Sigma, Cat# IPVH00010, USA) in 10x Tris/Glycine buffer (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... 50 µM mutant protein solution was mixed with excess amount of Atto dye in respective binding buffer (Sigma Aldrich) and incubated overnight at 4°C in a dark place ...
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bioRxiv - Biophysics 2024Quote: ... Refolded proteins were further purified by size-exclusion chromatography using a Hiload 16/600 Superdex 75 column (Sigma-Aldrich) in buffer A.
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bioRxiv - Plant Biology 2024Quote: ... Tissue was ground in ice-cold protein extraction buffer (150 mM NaCl, 50 mM Tris-HCl [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was filtered through polyvinylidene fluoride ultra-free membrane filter with a 0.22 μm pore size (Millipore). The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were incubated at 50 µM in the presence of 10x excess of Bromo-1,1,1-trifluoroacetate (BTFA, Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated to 2.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was eluted with GFB containing 2.5 mM desthiobiotin and concentrated in a 10 kDa MWCO concentrator (Millipore Sigma). Concentrated protein was loaded onto the Superdex 75 Increase 10/300 GL (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... CHIKV nsP1 protein was concentrated to the required concentration with Amicon centrifugal filters (10,000 MWCO Millipore, Burlington, MA, USA), flash-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins from the purified PB or pre-sorting fractions were dissolved in a 8 M urea solution (Sigma-Aldrich) before trypsin digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... The fractions that contained the SsCBE2-C2 protein were collected and concentrated through an Amicon Ultra centrifugal filter (Millipore). Following that ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were detected after incubating with peroxidase conjugated secondary antibodies for 1 hour and 5min with ImmobilonR Western (Millipore) after 5x washes ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing the expressed protein were combined and concentrated to 2.5 mL with a 30-kDa cut-off centrifugal filter (Millipore), run through the desalting column again ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated by 7.5% SDS-PAGE and transferred to an Immobilon-P membrane (Millipore, Billerica, MA, USA), as previously described (Towbin et al. ...
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bioRxiv - Cell Biology 2024Quote: 500 μg of crude cell lysate was precleared with 10 μl of PureProteome Protein A Magnetic Beads (Merck Millipore, Burlington ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates from the adipose tissue and serum samples were analyzed for inflammatory markers (Millipore Sigma #MCYTOMAG-70K-PMX) and adipokines (Millipore Sigma #MADMAG-71K-07 ...
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bioRxiv - Neuroscience 2024Quote: ... The eluted protein and the beads were separated by briefly spinning in a SpinX column tubes (Sigma CLS8162-24EA). The purity of purified protein was by running 5 µg of protein on an SDS PAGE gel and performing Comassie stain ...
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bioRxiv - Plant Biology 2019Quote: ... The purified protein was concentrated using an Amicon Ultra centrifugal filter with a molecular mass cut-off (MWCO) of 30 kDa (Millipore) and analysed on a Superdex 200 size-exclusion chromatography (SEC ...
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bioRxiv - Synthetic Biology 2021Quote: ... purified Sx-Cm-MOMP and Sx-CtE-MOMP proteins were spotted directly onto nitrocellulose membranes and incubated for 5 min before being blocked with 5% BSA (Sigma) and probing with the same primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... The initial rates of reaction were normalised to total protein content as determined using the QuantiPro BCA Assay Kit (Sigma). Control reactions without any substrate (G3P for GapA and GapN ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μl of Protein G magnetic beads were used and 10 μg of mouse monoclonal anti-FLAG primary antibody M2 (Sigma) were added before overnight incubation at 4°C with the lysate ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed on 2 mg of total protein by incubating 2h at 4°C with anti-Flag agarose beads (Sigma). Beads were then washed in lysis buffer and bound polypeptides analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg of protein per lysate were subjected to anti-FLAG immunoprecipitation using 15 ul of packed magnetic anti-FLAG beads (M2, Sigma) for 4h ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was diluted 10 times and incubated overnight at 4 °C with 20 µL Protein A/G MagnaChIP beads (Millipore) and 8 µg anti-H3K27me3 (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared from cell debris by centrifugation prior to adjustment of protein concentrations between the samples and overnight immunoprecipitation at 4° C with pre-equilibrated anti-HA-agarose (Sigma) or anti-c-myc-agarose (Thermo fisher) ...