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Citations for Millipore Sigma :
9851 - 9900 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... Hydrolyzed protein diet was prepared by incubating 5 mL intact protein diet with 43.7 mg trypsin (Trypsin Powder, Porcine 1:250, 85450C, Sigma Aldrich) at 37°C for 20 hours ...
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bioRxiv - Microbiology 2020Quote: ... Equal protein concentrations were loaded onto 12% SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (Millipore Corporation, Billerica, Mass.). Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... A volume of 12.5 μl of a solution containing 500 ng protein was diluted in H2O with 5× Sypro Orange (Sigma-Aldrich) and immediately mixed with an equal volume of assay condition ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed overnight at 37 °Cwith Lys-C (Wako) at an enzyme/protein ratio 1:75 and trypsin (Sigma) at an enzyme/protein ration of 1:50 ...
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bioRxiv - Biochemistry 2019Quote: ... The T1r2a/T1r3-LBD protein was purified from the culture medium by the use of ANTI-FLAG M2 Affinity Gel (SIGMA). The purified protein was dialyzed against the assay buffer (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2019Quote: Total protein was extracted from L4/5 DRGs or retinas using the RIPA buffer containing protease inhibitor cocktail (Sigma-Aldrich) and phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... and equal amounts of protein were analyzed using the following antibodies: anti-FLAG M2 (Sigma, cat#F3165, RRID:AB_259529; 1:7,500), anti-IE1 (clone 1B12 (Zhu et al. ...
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bioRxiv - Microbiology 2019Quote: Bacterial cells were removed by centrifugation and culture filtrate protein (CFP) was prepared by filtering the supernatant through 0.2 µM pore size filters (Millipore, USA). After sterility testing of CFP in Mycobacteria Growth Indicator Tube (MGIT ...
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bioRxiv - Plant Biology 2019Quote: ... The presence of immunocomplexes was assessed by probing protein gel blots with either anti-HA (3F10, Sigma/Roche; http://www.sigmaaldrich.com/) or anti-Myc antibody (9E10 ...
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bioRxiv - Microbiology 2019Quote: ... Western immunoblotting was performed by incubating the membrane with Streptavidin HRP-linked protein in PBS-BSA buffer and developing with TMB (Sigma).
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bioRxiv - Cancer Biology 2020Quote: CAF CMed was also separated into the metabolite (less than 3kDa) and protein (more than 3kDa) fractions using 3kDa centrifugal filters (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Protein samples were diluted to 1 M urea with 50 mM TEAB buffer and digested with 1:100 trypsin (Sigma) 2 h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 4 mg of lysate proteins were incubated with 40 μl of pre-washed anti-FLAG M2 magnetic beads (Sigma– Aldrich) for 2 h ...
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bioRxiv - Physiology 2019Quote: ... cells were washed twice with ice cold D-PBS and proteins harvested using RIPA buffer supplemented with cOmplete protease and PhosStop inhibitors (Sigma). The lysates were cleared by centrifugation at 13 000 rpm for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Beads were washed extensively in lysis buffer and bound proteins eluted in 500 µL 100 µg/ml 3X FLAG peptide (Sigma) in 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... Buffer exchange to PBS and the concentration of the protein were done by using amicons® (Merck Millipore Ltd, cat:UFC900324).
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bioRxiv - Biophysics 2020Quote: ... The protein was then concentrated to 500 μL using a 50 kDa cutoff Amicon Ultra-4 Centrifugal filter (Millipore UFC805008) and injected onto a Superdex 200 increase gel filtration column in sizing buffer (25 mM HEPES pH 7.0 ...
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bioRxiv - Immunology 2021Quote: ... A total of 105 cells or 20 mg protein was resolved by SDS-PAGE and transferred to Immobilon-P PVDF membranes (Millipore). Membranes were blocked in 5% milk in TBS-T (25 mM Tris-HCl [pH 7.4] ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of protein were loaded and separated in 12% SDS-PAGE gels and transferred to Immobilon-FL PVDF membranes (Millipore). Membranes were blocked for 1 hour at room temperature with Intercept (TBS ...
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bioRxiv - Genetics 2020Quote: ... Proteins were concentrated using an Amicon Ultra-0.5 mL centrifugal filter device with a cutoff of 100 kDa (Millipore Sigma).
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bioRxiv - Biochemistry 2020Quote: ... The protein was then concentrated by centrifuging at 5000 RPM in an Amicon® Ultra-15 10K centrifugation column (Millipore). Protein concentration was measured by UV absorbance at 280nm using an Ultraspec 2100 pro by Amersham Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... Free dye was removed from the labeled proteins by successive concentrations and dilutions using a 3 K MWCO centrifugal concentrator (Amicon Ultra-4, Millipore). The labeling efficiency was computed by measuring the concentration of the protein and Cy3 dye using their absorbances at 280 nm and 550 nm ...
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bioRxiv - Immunology 2021Quote: ... the semi-dry protein pellet was dissolved in 25µL of 8 M urea in 20mM EPPS (pH 8.5) (Sigma; Cat#E9502) and was then diluted with EPPS buffer to reduce urea concentration to 4 M ...
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bioRxiv - Immunology 2021Quote: ... Samples of the eluted fractions were loaded into 15% SDS-PAGE gels and the fractions containing the nucleocapsid protein were concentrated using Amicon Ultra-15 concentric filters (Merck Millipore) with a 10 kDa cutoff ...
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bioRxiv - Neuroscience 2020Quote: ... Samples with equal amounts of total protein were separated in 11% SDS-polyacrylamide gel before electrophoretic transfer onto Immobilon-P membranes (#IPVH00010; Millipore). Membranes were cut horizontally ...
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bioRxiv - Biochemistry 2021Quote: ... 1mL (2 mg) of protein was added to 20 µL of ANTI-FLAG® M1 Agarose affinity Gel (Sigma Aldrich) pre-equilibrated in TBS and incubated on a nutator for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Bound protein was eluted seven times with 0.5 column volumes of buffer A with 3 mM D-Desthiobiotin (Sigma-Aldrich), 0.026% DDM and 0.1 mM AEBSF protease inhibitor ...
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bioRxiv - Immunology 2021Quote: Approximately 5×105 cells stably expressing the indicated TurboID-V5-fusion proteins were treated as described prior to the addition of 500μM biotin (Sigma-Aldrich) for 30min ...
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bioRxiv - Bioengineering 2020Quote: ... For western blotting 40 μg of protein samples were subjected to 10% in house SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 3% BSA in TBS containing 0.01 % Tween-20 for 1 h at room temperature and then incubated with specific primary antibodies at 4 °C overnight in the blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: To expose Prdx2 to continuous H2O2 generation the protein (20 μM) was incubated with 5 mM glucose plus glucose oxidase (Sigma). Glucose oxidase was diluted to produce approximately 0.5 μM H2O2/min.
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing >90% pure protein (evaluated by SDS-PAGE) were concentrated using an Amicon Ultra-15 centrifugal unit (EMD Millipore) and flash frozen in liquid nitrogen before storage at −80°C.
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bioRxiv - Biochemistry 2020Quote: ... Each protein encoding DNA fragment was assembled with the mCherry-PCR fragment and NdeI and HindIII digested pET22b vector (Novagen), using a Gibson assembly reaction (New England Biolabs) ...
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bioRxiv - Biochemistry 2020Quote: A protein/Sypro orange mix containing 5 μM protein and a 1:5,000 dilution of dye in DMSO (as supplied by Sigma Aldrich) was prepared just before plate setup in 20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant protein concentration was assessed by quantification of the change in absorbance of the Bradford reagent (Sigma-Aldrich, USA) at 595 nm utilizing Infinite F200 PRO multimodal microplate reader (Tecan ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were sequentially incubated with primary and HRP-coupled secondary antibodies and proteins were detected using chemiluminescence reagents (Luminata Crescendo, Millipore) and X-ray films ...
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bioRxiv - Neuroscience 2021Quote: ... a total of 4 μg of S protein diluted in 12 μl sterile ddH2O containing hyaluronidase (100 UI; Sigma-Aldrich) were infused in the intranasal cavity of mice ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and nsp12 proteins were subsequently detected using the mouse monoclonal anti-DYKDDDDK M2 antibody (Catalogue number F3165, Sigma-Aldrich) diluted 1:1000 in TBST/5% milk ...
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bioRxiv - Cell Biology 2021Quote: ... 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma) were added to each sample for 3.5 hours at 4°C after the overnight incubation with antibody ...
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bioRxiv - Biophysics 2021Quote: ... The protein was expressed and purified the same way as SARS-CoV Mpro-2 but Factor Xa protease (Sigma, Canada) was used (4 °C ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). For PDAC cells grown as 3D organoids in co-culture ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). Organoids were grown in complete media when passaging ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein samples were subjected to SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, MA, USA). The membranes were treated with 1% blocking solution in TBS for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... 25 µg of the protein was loaded in 4-12% Bis-Tris gradient polyacrylamide gel and transferred onto PVDF membrane (Millipore). Membranes were blocked in PBS with 0.1% Tween 20 and probed with respective primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein (11 µg) were run on a 10% SDS-PAGE gel and transferred to a polyvinylidene difluoride membrane (Millipore) overnight ...
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bioRxiv - Plant Biology 2021Quote: Total PME activity was quantified on cell wall-enriched protein extracts using commercial citrus pectins (DM >85% P9561, Sigma-Aldrich) and the alcohol oxidase-coupled colorimetric assay (Klavons and Bennet ...
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bioRxiv - Plant Biology 2021Quote: ... Chromatin samples were incubated with 50 ml of washed Protein G Dynabeads pre-coated with 1mg of S9.6 antibody (Millipore MABE1095) for 16h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the protein were pooled and concentrated with an Amicon Ultracell Centrifugal filter unit (MWCO 50 kDa, Merck Millipore) to a volume of 500 µL and digested with TEV protease for 2 hours at 8°C ...