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Citations for Millipore Sigma :
9501 - 9550 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by 12% SDS-polyacrylamide gel electrophoresis (PAGE) and transferred by electroblotting onto polyvinylidene difluoride membrane (Millipore). After blocking 1 h with 5% nonfat dry milk ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were reduced and alkylated after treatment with 10 mM DTT (Astral Scientific) and 20 mM chloroacetamide (Sigma). The gel was dehydrated using 100% ACN and rehydrated with digestion solution ...
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bioRxiv - Microbiology 2021Quote: ... Fractions containing each DNA pol of a high purity were pooled and concentrated using a 10K protein concentrator (Millipore). Concentrated proteins were preserved at −20 °C in the presence of 50% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... the eluted proteins were loaded to 12% SDS-PAGE gel and then transferred to PVDF membranes (Millipore, Boston, USA). After blocking with 5% non-fat milk for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... 15 mL protein extracts were incubated in a 50 μL slurry of anti-FLAG M2 affinity gel (Sigma, A2220) and rotated at 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... coli total protein extract were spotted within a pre-marked grid onto a 0.2 μm pore–size nitrocellulose membrane (Merk Millipore). The membrane was left to dry to fix the proteins for 30 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The epithelial cell proteins that remained bound to the fungal cells were eluted twice with 6 M urea (Sigma). The proteins were then separated on 10% SDS-PAGE and transferred to immun-Blot PVDF Membrane (BIO-RAD) ...
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bioRxiv - Molecular Biology 2021Quote: RNA immunoprecipitation assay was carried out according to the Magna RIP RNA-Binding Protein Immunoprecipitation Kit manufacturer’s instruction (Millipore). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated with 381.7 ng / ml mouse α-HAdV hexon protein antibody (Mab8052, Sigma-Aldrich, St. Louis, USA) and subsequently stained using 2 μg / ml goat α-mouse-AlexaFluor594 (A21203 or A32742 ...
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bioRxiv - Cancer Biology 2020Quote: Protein co-localization was assessed via proximity ligation assay (PLA) using the Duolink In Situ Red Starter Kit (Sigma) and PLA-approved primary antibodies for SC-35 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... protein gel pieces were digested overnight with trypsin (Pierce) following reduction and alkylation with DTT and iodoacetamide (Sigma–Aldrich). The samples then underwent solid-phase extraction cleanup with a Oasis HLB μElution plate (Waters ...
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bioRxiv - Cell Biology 2021Quote: ... the alpacas were immunized subcutaneously and intradermally with 3 mL of 0.5 mg CTLA-4 protein and an equal volume of complete Freund’s adjuvant (0.5 mg; Sigma–Aldrich, USA). On days 91 and 112 ...
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bioRxiv - Immunology 2021Quote: ... the eluted proteins were loaded onto a 12% SDS-PAGE gel and then transferred to a PVDF membrane (Millipore). After blocking with 5% nonfat milk for 1 h ...
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the capsid protein were pooled and concentrated using an Amicon Ultra-15 centrifugal filter (Merck Millipore), and the buffer was exchanged to a final storage buffer composed of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Biophysics 2021Quote: ... The final purified proteins were concentrated using 50 kDa (MtCNNMΔC) or 100 kDa (MtCNNM) cutoff concentrators (Amicon Ultra, Millipore), flash frozen in liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 4.5–5.5 mg/mL (Amicon Ultra 100-kDa cut off, Millipore Sigma). Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (Implen Nanophotometer N60 ...
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bioRxiv - Biophysics 2021Quote: ... Further purification was achieved through capture of the cleaved protein by Strep-Tactin resin (IBA) and elution with 2.5mM d-desthiobiotin (Sigma). A final purification step was performed by size-exclusion chromatography using a HiLoad 16/60 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: The protein was thawed and concentrate at 10 mg/ml in Amicon Ultra-15,10000Mr cut-off centrifugal concentrator (Millipore). 10 mM shikonin was added to the protein in a ratio of 5:1 and bond at 4°C for 2h ...
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bioRxiv - Biophysics 2022Quote: ... Protein was concentrated up to 40–60 mg/ml using a 100 kDa molecular weight cut-off concentrator (Millipore). The protein purity was checked by SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified affinity chromatography using Ni-NTA column (Yeasen, Wuhan, China) and concentrated using centrifugal ultrafiltration (Millipore). The concentration of recombinant protein was determined with bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2022Quote: ... Non-specific protein adsorption to the coverslip was prevented by adding 0.005% (v/v) Tween-20 (P2287, Sigma-Aldrich) to the solution ...
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bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
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bioRxiv - Microbiology 2022Quote: RNA immunoprecipitation (RIP) assays were performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (EMD Millipore 17-700). HEK293T cells were plated on 15-cm dishes coated with poly-L-ornithine at a density of 4E4 cells/cm2 ...
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bioRxiv - Biochemistry 2022Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Sigma) or 16O light (Fisher ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... were used at 14,000 x g and 4 °C to exchange purified protein samples to 150 mM ammonium acetate (99.99 %; Sigma-Aldrich), pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Argonaute proteins/small RNA complex were extracted from 50,000 synchronized worms and immunoprecipitated with anti-flag antibody (Sigma Aldrich) or GFP nanobody (Thermo) ...
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bioRxiv - Microbiology 2022Quote: ... of 3.5 mg/mL mixtures of purified STFK or STFK1628x proteins in complex with excess Fab fragments of nAbs were incubated in 0.01% (v/v) Digitonin (Sigma) and then loaded onto glow-discharged (60 s at 20 mA ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated by SDS-PAGE with 8% polyacrylamide gel and transferred to PVDF membrane (Millipore, Billerica, MA, USA). Membranes were blocked with 5% skim milk in Tris-buffered saline (20 mM Tris [pH8.0] ...
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bioRxiv - Immunology 2022Quote: Antibodies against the following proteins were obtained from commercial sources and used for immunoblots: anti-flag-tag (F3165, Sigma), anti-V5-tag (R960-25 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell types were probed with anti-Pan cytokeratin (Cat # 4545, Cell signalling technology, U.S.A) and anti-Fibroblast specific protein (Cat # F4771, Sigma, U.S.A) as primary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions of monomeric protein were collected and concentrated to around 30-50 µM using Amicon Ultra-0.5 ml Centrifugal Filters (Millipore), then separated into single-use aliquots ...
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bioRxiv - Bioengineering 2022Quote: ... The prenylated protein was subsequently purified by buffer exchange (PBS) with an Amicon Ultra-0.5 centrifugal filter (10 kDa cutoff, Millipore) for cell surface modification or the following conjugation reactions.
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bioRxiv - Bioengineering 2022Quote: The proteins and protein conjugates were washed into ultrapure water with the Amicon Ultra-0.5 centrifugal filters (10 kDa cutoff, Millipore). 50 μL of each protein (5 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were eluted by incubating the beads with 35 μl 100 µg/ml 3xFLAG-Peptide (F4799, Sigma Aldrich) at room temperature for 20 min or by adding Laemmli Sample Buffer (1610747 ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was recovered and the protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Merck-Millipore). 400 µg protein were incubated with 40 µL of streptavidin coupled DynabeadsTM M-280 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Protein extracts were separated by SDS-polyacrylamide gel electrophoresis then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Temecula, CA). Primary antibodies and secondary antibodies were used according to manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... The protein-containing supernatants from cells expressing HA-I53_dn5B fusion proteins were further clarified by vacuum filtration (0.22 µm, Millipore Sigma). Prior to immobilized metal affinity chromatography ...
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bioRxiv - Immunology 2020Quote: ... The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich) and 0.1% Tween-20 ...
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bioRxiv - Biochemistry 2021Quote: ... After elution with imidazole the purified protein was concentrated using a 10 kDa MW cutoff Amicon centrifugal filter (Sigma), buffer exchanged into PBS (10mM Na2HPO4 ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was washed 3 times in a solution of 0.3 M guanidine hydrochloride (Sigma, Cat. No. SRE0066) in 95% ethanol ...
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bioRxiv - Neuroscience 2019Quote: ... proteins were reduced with 20 mM dithiothreitol and free cystein residues were alkylated with 50 mM iodoacetamide (Sigma Aldrich). After the urea washing steps ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing Galectin-3 protein was treated with benzamidine sepharose (HiTrap Benzamidine FF, Sigma, Cat# GE17-5143-02) to bind and remove thrombin ...
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bioRxiv - Developmental Biology 2020Quote: ... the supernatant was subjected to an immunoreaction with a specific antibody pre-conjugated with magnetic protein A beads (Millipore) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing the complex were concentrated to 0.12 mg/ml and proteins were crosslinked by addition of 0.05% (v/v) glutaraldehyde (Sigma Aldrich) and incubated at room temperature for 60 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Extracted proteins were then separated via SDS-PAGE and then transferred to a 0.45 μM polyvinylidene fluoride (PVDF) membrane (Millipore) on a TRANS-BLOT SD apparatus (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein content in each eluate was measured using infra-red spectroscopy (Direct Detect, Merck Millipore, Burlington, MA, USA). The remainder was aliquoted and stored at −80 °C.
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of total protein were separated by 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were blocked and incubated with primary antibodies against FGF2 (36769 ...
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...