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Citations for Millipore Sigma :
9651 - 9700 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The eluate was then dialyzed overnight in PBS and protein was concentrated by using Amicon Pro centrifugal filter (Millipore). The protein was quantified by using BCA Protein Assay Kit (Thermofisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Size standards were generated using a gel filtration marker kit of protein molecular weights that ranges from 29,000 to 700,000 (Sigma-Aldrich).
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bioRxiv - Plant Biology 2022Quote: ... immunoblotting was performed with 50 μg of protein extracts using anti-Flag-M2-HRP (Sigma-Aldrich, A8592; 1:10,000), anti-c-myc-peoxidase (Roche ...
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Local induction of IAA5 and IAA29 promotes DNA damage-triggered stem cell death in Arabidopsis rootsbioRxiv - Plant Biology 2022Quote: ... Chromatin bound to SOG1-Myc proteins extracted from whole seedlings was precipitated with anti-Myc antibody (clone 4A6, Millipore). To quantify the precipitated chromatin ...
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bioRxiv - Plant Biology 2022Quote: ... Then total proteins were resolved in 8% SDS-PAGE and transferred to immobilon-P membrane (MILLIPORE cat. No. IPVH00010). GFP antibody (trans #HT801) ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 M NaCl) before eluting bound proteins with buffer A containing 0.5 M Methyl-α-D mannose (Sigma). Fractions containing NA activity were pooled ...
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bioRxiv - Microbiology 2023Quote: ... and proteins were concentrated by 30 kDa cut-off Amicon Ultra-15 Centrifugal Filter Units (Millipore, Cat. No. UFC903096).
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bioRxiv - Immunology 2022Quote: ... Protein extracts were loaded onto 8/15% polyacrylamide gels and then transferred onto a PVDF membrane (Millipore, Burlington, MA). Membrane was then blocked by incubating in 5% skim milk/TBS for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Protein expression from hybrid lac promoters was induced by addition of isopropyl-b-D-thiogalactopyranoside (IPTG 0.25 mM; Sigma) where indicated.
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bioRxiv - Physiology 2022Quote: ... 80 μg of protein were incubated in 10 mM L-cysteine and 1 mM pyridoxal 5′-phosphate hydrate (Sigma); or ...
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bioRxiv - Molecular Biology 2022Quote: ... The filters were blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.05% Tween (TBS-Tween) and then incubated overnight with antibodies directed against specific proteins: AMPK (07-181, Millipore), p-AMPK (#2535 ...
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bioRxiv - Plant Biology 2022Quote: ... Either total membrane or immunoprecipitated proteins were separated on a SDS-PAGE gel and transferred to PDVF membrane (Millipore) for immunoblot analysis using monoclonal anti-GFP (33-2600 ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysates were incubated with 1.5 µg polyclonal anti-V5 or IgG for 1 h and captured with protein A agarose resin (P2545, Sigma) for 30 min ...
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bioRxiv - Systems Biology 2022Quote: ... The lysate was centrifuged at 4,000 xg for 5 min at 4°C and the protein pelletwas resuspended in Digestion Buffer(10% 2,2,2-Trifluoroethanol (Sigma; #96924)) in 100 mM HEPEs pH 8.5) ...
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bioRxiv - Molecular Biology 2022Quote: ... Nanobody fusion proteins were detected using a primary anti-HA (mouse; 1:3,000, Sigma-Aldrich, St. Louis, MO, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2022Quote: Cleaved protein was concentrated to ~1 mL using an Amicon Ultra-15 centrifugal filter 30 kDa MWCO (EMD Millipore), then loaded onto a HiLoad Superdex S200 pg gel filtration column (GE Healthcare Life Science) ...
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bioRxiv - Molecular Biology 2022Quote: ... Total protein (20 μg) was electrophoresed on 15% SDS-polyacrylamide gel and transferred to PVDF (Millipore, Burlington, MA, USA) membranes ...
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bioRxiv - Cell Biology 2022Quote: ... followed by treatment with protein synthesis inhibitor cycloheximide (CHX) (Cat# C-7698, Sigma, stock 100 mg/ml in DMSO) at 0.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Supernatants were collected at 42-48 h after transfection and filtered through a 0.45 um low-protein binding filter (Millipore). Patient-derived TCL were infected with lentiviral supernatants containing polybrene (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... the protein was eluted with 5 ml buffer B containing 15 mM D-desthiobiotin (Sigma Aldrich, St. Louis, USA). After purification ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was identified by its monomeric molecular weight (33.55 kDa) and concentrated using an Amicon 10 kDa concentrator (Millipore). The concentrated solution was therefore loaded onto a HiLoad 26/600 Superdex 200pg column (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0) supplemented with 1X Protein Inhibitors Cocktail (SERVA) and a tip of spatula of DNAse I (Sigma Aldrich). Bacterial cells lysis was performed by sonication ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the equivalent of 500-800 µg of protein was incubated with 1 µg of anti-GFP antibody (Sigma-Aldrich) and then incubated with Protein A/G magnetic beads (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluates were then desalted and concentrated by ultrafiltration with Amicon Ultra 0.5 10K (Merck Millipore, Burlington, Massachusetts, USA) columns ...
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bioRxiv - Neuroscience 2024Quote: ... to perform immunohistochemical procedures in the Penn Digital Neuropathology Lab as described previously.21,61 Immunohistochemistry for neuronal markers included antigen retrieval using citrate buffer and heat for neuronal nuclei protein (NeuN) enriched in most neurons (Anti-NeuN mouse monoclonal antibody, clone A60,1:1000, Millipore) and heavy non-phosphorylated neurofilament (NF-H ...
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bioRxiv - Neuroscience 2024Quote: Total protein from UM51- and B4-derived neuronal cultures was isolated using RIPA buffer (Sigma-Aldrich Chemicals, Taufkirchen, Germany), supplemented with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Genetics 2024Quote: ... 1mg of cell lysate was incubated with blocked Protein G beads and monoclonal L1 ORF1p antibody (EMD Millipore, MABC1152) at 12 rpm at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... The purified protein was concentrated to ∼1 mg/mL by centrifugal filtration (AmiconTM 30K, Millipore Sigma, Burlington, MA, USA), flash frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The fractions containing the purified protein were pooled together and concentrated using 30-kDa cut-off centrifugal devices (Millipore). Further ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins (20-40 μg) were subjected to 10% SDS-PAGE and electrotransferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore). After incubation in a blocking buffer (5% bovine serum albumin/TBST ...
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bioRxiv - Synthetic Biology 2023Quote: ... The quality of protein transfer was visualized by staining with Ponceau S (0.1% dye in 5% acetic acid, Sigma) and then blocked with 5% nonfat instant milk (Carnation ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein samples were run on EZ Protein any KD PAGE (LIFE-iLAB) and then transferred to 0.22um PVDF membranes (Millipore). Membranes were blocked using 5% non-fat milk in PBS-Tween20 0.1% for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 500-1000µg of total protein was incubated with 20-40 µl of prewashed anti-FLAG agarose beads (Sigma, #A2220) for 3h at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cells were lysed on ice for 30 min in the manufacturer’s lysis buffer supplemented with 0.5% Triton-X100 and complete EDTA-free protein inhibitors (Sigma-Aldrich), with gentle 5s vortexing every 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated on 6-10% SDS-PAGE gels and transferred to methanol pre-activated PVDF membranes (Millipore, #IPVH00010). For MYCBP2 blots ...
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bioRxiv - Cancer Biology 2024Quote: ... conditioned media was isolated and filtered through a 0.4µm low protein binding PVDF Miltex syringe-driven filter (Millipore #SLHV033RS) to remove cellular debris ...
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bioRxiv - Cancer Biology 2024Quote: ... Conditioned media was isolated and filtered through a 0.4µm low protein binding PVDF Miltex syringe-driven filter (Millipore #SLHV033RS) to remove cellular debris ...
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bioRxiv - Plant Biology 2023Quote: ... An equal amount of protein was used for immunoblot analysis using an anti-HA antibody (1:1000 dilution, Sigma) and anti-cMyc antibody (1:1000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL of this dilution was then added to 90 μL of buffered D2O (prepared 5 mL with 4.5 mL of D2O and 0.5 mL of 10x protein dilution buffer and 0.2 μg/mL of peptide standard Glu-1-Fibrino peptide (CAS: 103213-49-6, Sigma)) to initiate deuteration at 22 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein labeling was done by incubation with appropriate primary antibodies as indicated (Supplementary Table S1) in 5% BSA (Sigma) at 4°C overnight ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436 ...
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bioRxiv - Biochemistry 2023Quote: Equal amounts of protein from cell lysates were subjected to SDS-PAGE and transferred to a PVDF membrane (Millipore). HeLa GFP and HeLa TRAP1-GFP cells were treated with either 2 μg/mL of harringtonine and 100 μg/mL of emetine for 15 min at 37°C to inhibit cytosolic translation or 30 μM linezolide or 200 ng/mL chloramphenicol for 1 hr at 37°C to inhibit mitochondrial translation ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells (100% confluent) were harvested and lysates prepared using CytoBuster protein extraction reagent (Novagen/EMD, Gibbstown, NJ) supplemented with complete mini EDTA-free protease inhibitor (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Different amount of protein samples were electrophoresed on 10% SDS-PAGE and then transferred to PVDF membranes (Millipore, Merck) by electroblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were resuspended in 150µL wash buffer 3 and diluted with two volumes of 10 mM Tris pH 8.5 prior to on-bead digestion of proteins via addition of 500 ng modified sequencing grade Trypsin (Sigma) with incubations of 1 hour at 4°C and then overnight at RT with continuous mixing at 1200 rpm ...
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bioRxiv - Neuroscience 2023Quote: The eluent containing the target proteins underwent filtration using a Centricon filter with a 100 kD cutoff (EMD Millipore). The Centricon filter was then centrifuged at 1,200g for 10 minutes until the sample volume was reduced to less than 1 mL ...
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bioRxiv - Plant Biology 2023Quote: Frozen leaf tissue was ground in two volumes of protein extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.1% Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2023Quote: ... and 100 µM Na3VO4) before the protein was eluted with FLAG wash buffer containing 0.5 mg/mL 3xFLAG peptide (Sigma). LIMK1 concentration was determined by running alongside BSA standards on SDS-PAGE with Coomassie staining.
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Cancer Biology 2023Quote: ... 210 ug of protein was digested using Lys C (Wako) at an enzyme:protein ratio of 1:100 and trypsin (Sigma) at 1:50 ratio overnight at 37 °C ...