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Citations for Millipore Sigma :
9901 - 9950 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... After 5 h of incubation the culture medium was de-proteinized by adding an equal volume of 3% trichloroacetic acid (Sigma # T6399) and incubation at 50 °C for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... 1.4 OD of cells were pelleted by centrifugation at 2500 g for 3 min and resuspended in 130 μL X-gal reagent (0.1% (w/v) X-Gal (Sigma-Aldrich, #3117073001), 0.05% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 x 10 min in PBS and incubated for 1 h in PBS containing 10 mg/mL sodium borohydride (Sigma, 213462) to quench autofluorescence ...
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bioRxiv - Cancer Biology 2024Quote: ... Plain media was added overnight and on day 3 cells were passaged into selection media containing 2 µg/uL puromycin (Sigma P8833) and 150 µg/mL hygromycin B (Thermo 10687010 ...
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bioRxiv - Bioengineering 2024Quote: Hydrogels for cell culture experiments were fabricated by placing 90-µL drops of precursor solution between a 3-(trimethoxysilyl)propyl acrylate (Sigma-Aldrich) silanated 18-mm glass coverslip and a glass slide treated with SigmaCote (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... These double transgenic animals were separated into 4 groups (n = 3) and injected with a single dose of 4OH-Tx (Sigma-Aldrich). Tissue was collected at 10- ...
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bioRxiv - Physiology 2024Quote: ... hearts were prepared for microscopic studies by immediate immersion into 3 M KCl to stop its function in diastole and placed into 20% sucrose (Sigma Aldrich) for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... after which the rats in the ACTH group (n=50) were administered with either 3 injections of imipramine (n=10) (IMI, 15 mg/kg i.p. (Sigma-Aldrich, Denmark)) or 1 injection of saline ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was changed every 3–4 d and enriched with granulocyte-macrophage colony-stimulating factor (5 ng/ml GM-CSF, Sigma-Aldrich). After approximately 14 d at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: Three pairs of Day 56 forebrain organoids derived from iPSCs of three CTRL and WS patients were dissociated with 3 mL Tryp-LE (Sigma-Aldrich) for 15 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The SE lipid mixture of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine): DPC (dihexadecyl phosphate, a negatively-charged lipid): CH (cholesterol) (catalog #s: Avantis Polar Lipid 850345P, Sigma Aldrich 2197-63-9 ...
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bioRxiv - Cancer Biology 2024Quote: Tumours and control tissues (comparable anatomical region) were harvested and dissociated using a hand-held tissue homogenizer (Omni International Inc.) in 2-3 ml RNAzol (Sigma, 4533). Total RNA was isolated using RNAzol ...
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bioRxiv - Cell Biology 2024Quote: ... After the last EtOH wash the coverslips were incubated with methanol containing 5% acetic acid and 1% (3-Aminopropyl)triethoxysilane (Millipore-Sigma) overnight in a vacuum desiccation chamber in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Custom 3D printed cavity was filled with fluorescein dye-infused with 3% agar gel (10% v/v, catalog no. F2456; Sigma Aldrich). The cranial implant was then placed within a mold made on the open surface of the cavity that leveled the top surface of the implant with the top surface of the cavity and filled up the entire bottom transparent PET window surface with fluorescent gel ...
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bioRxiv - Neuroscience 2024Quote: ... sciatic nerves were harvested and fixed for 1 h in 4% paraformaldehyde (PFA) followed by 15 min immersion in a 3% sodium fluorescein (NaFlu, molecular weight: 376 Da; Sigma Chemicals) in saline solution 2 ml of Evans blue albumin (EBA ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by a series of washes in PBST (PBS with 3% Triton™ X-100, Sigma-Aldrich, X-100) (once for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with TBS-T (TBS + 0.05% Tween20) (3 times x 2 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Biophysics 2024Quote: Adenosine 3’,5’-cyclic monophosphate sodium salt monohydrate (cAMP) and guanosine 3’,5’-cyclic monophosphate sodium salt (cGMP) were purchased from Sigma Aldrich and both prepared at 16 mM in 150 mM KCl ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by dilution of the supernatant with fresh medium in a ratio of 1:1 and addition of 3 µg/mL hexadimethrine bromide (polybrene, Sigma-Aldrich). 5 µg/mL Puromycin were added and maintained to select for positively transduced cells ...
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bioRxiv - Biochemistry 2024Quote: ... The duplex samples were then buffer exchanged into NMR buffer using Amicon Ultra-15 centrifugal concentrators (3-kDa cutoff, Millipore Sigma) at least 3 times ...
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bioRxiv - Biochemistry 2024Quote: ... Lipids from bovine brain extract (Folch fraction 1) and 16:0/16:0-PI(3)P were purchased from Sigma Aldrich and Echelon Biosciences ...
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bioRxiv - Cell Biology 2024Quote: Cell differentiation was conducted using previously described protocol 32 with slight modifications by using sequential inhibition of GSK-3 by CHIR99021 (8 µM, Sigma-Aldrich) and WNT by IWP-2 (5 µM ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were dissected into fragments approximately 9 mm3 in size and placed in glyoxal acidic solution (3% w/v glyoxal [Millipore Sigma 128465], 20% ethanol [Fisher BP2818100], 0.75% acetic acid [Millipore Sigma A6283] ...
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bioRxiv - Microbiology 2024Quote: ... cells in each culture plate were transfected with 10µg of pNL4-3 plasmid DNA complexed with 50μL PEI reagent (Sigma-Aldrich catalog # 764604), and the culture media was changed 16 hours post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... The suspension was transferred to FACS tubes cells were washed twice with FACS-buffer (DPBSdef + 3 % foetal calf serum (Sigma-Aldrich) + 2 mM EDTA (Roth ...
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bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
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bioRxiv - Immunology 2024Quote: ... Viral transduction was performed by spinfection at 900 g at 25 °C for 3 h with 10 μg/ml polybrene (Sigma-Aldrich), followed by resting for 3 h at 37 °C in 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... the auxin-inducible degron was induced by adding 500 μM indole-3-acetic acid (IAA, chemical analog of auxin) (Sigma, I5148) to the media ...
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bioRxiv - Cell Biology 2024Quote: ... After rinses with 0.1 M PBS followed by dipping in Milli-Q water (DirectQ® 3 UV water purification system, Millipore, MA). Sections were air-dried and cover slipped with Prolong® diamond antifade mounting medium (Cat ...
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bioRxiv - Immunology 2024Quote: ... The filtered supernatant was then concentrated using a centrifugal filter unit with a 3 kDA cutoff at 4°C (Amicon Ultra-4 Centrifugal filters, Millipore, USA). Concentrated supernatant was stored in 200 µL aliquots at −80°C until further analysis.
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bioRxiv - Molecular Biology 2024Quote: ... A total of 4×108 cells were first fixed with 3% pFA at the culture temperature for 10 minutes and subsequently with 10 mM dimethyl adipimidate (DMA; Sigma, 285625) at room temperature for 45 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Protein knock-downs using the mAID system were performed under 500 µM auxin (IAA: 3-indoleacetic acid in 100% ethanol; Sigma-Aldrich) [64].
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bioRxiv - Molecular Biology 2024Quote: Embryos were anesthetized in 0.016% tricaine methane-sulfonate (Pentair, Apopka, FL) and mounted in 3% methylcellulose (Sigma-Aldrich, St. Louis, MO). Embryos were imaged using Leica Thunder Imager M205 FCA ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were dissected into fragments approximately 9 mm3 in size and placed in glyoxal acidic solution (3% w/v glyoxal [Millipore Sigma 128465] ...
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bioRxiv - Neuroscience 2024Quote: Incubation chambers were set up to submerge slices in oxygenated (95% O2, 5% CO2) aCSF containing a selection from the following drugs: 3 µM puromycin dihydrochloride (Sigma P8833), 100 µM dopamine hydrochloride (Sigma H8502) ...
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bioRxiv - Systems Biology 2024Quote: The filtrate was lyophilized and suspended in 50 μL of water containing the reference compounds [200 μM each of trimesate (206-03641, Wako) and 3-aminopyrrolidine (404624, Sigma-Aldrich)] ...
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bioRxiv - Genetics 2024Quote: ... alkylated proteins were pre-digested for 3 hours at RT using 0.2 µg LysC after which the sample was diluted by adding 3 volumes of 50 mM ammoniumbicarbonate prior to overnight digestion with AspN at 37°C in 1mM methylamine (Sigma-Aldrich). Digests were diluted 1:1 with 2% trifluoroacetic acid and stored at -20°C prior to shotgun proteomics analysis.
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bioRxiv - Microbiology 2024Quote: All variants were prepared at a concentration of 3 µM with a final concentration of 10X SYPRO Orange Protein Gel Stain (Sigma-Aldrich) in a white ...
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bioRxiv - Microbiology 2024Quote: ... The cells were maintained with DMEM+10%FCS for 3 hours and fixed with acetone/methanol (1:1 ratio) and stained with Giemsa stain (Sigma-Aldrich). Stained cell images were taken by EVOS XL imaging system (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... ultrapure water was added and the suspension was ultrafiltered using an Amicon Ultra-0.5 3 kDa cut-off ultrafiltration tube (Merck Millipore, Massachusetts, USA). The ultrafiltrate was concentrated using a SpeedVac vacuum concentrator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Degradation of EGFP-AID-STAG2 was induced by the addition of S00 µM Inole-3-acetic acid (IAA, ISl48, Sigma-Aldrich) for 2 hours at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: CB6F1 mouse ovaries were removed from the bursa and placed in L15 medium supplemented with 3 mg/ml polyvinylpyrrolidone (PVP, P0930, Sigma-Aldrich) and 0.5% Pen-Strep ...
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bioRxiv - Synthetic Biology 2024Quote: ... filtered spent media collected from a confluent flask) at the ratio of 1:3 to fresh medium with 25% FBS and 1% penicillin-streptomycin (Penicillin-Streptomycin, Sigma Aldrich). Confluent wells were then assessed with fluorescence microscopes ...
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bioRxiv - Synthetic Biology 2024Quote: ... The strain was transformed with a plasmid containing a 3-methyl benzoate (3MB; m-Toluic Acid; Sigma Aldrich Catalog No. T36609) inducible recT recombinase and mutLE36K (pTE583 ...
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bioRxiv - Microbiology 2024Quote: ... respectively) by dilution in a vehicle solution of 10% w/v 2-hydroxypropyl-!3-cyclodextrin (Sigma-Aldrich, St-Louis, MO, USA) in PBS ...
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bioRxiv - Immunology 2024Quote: Cells obtained by crawl-out were incubated for 24h at 37°C with the phosphatidylinositol-3-kinase inhibitor wortmannin or the carnitine palmitoyltransferase-1 inhibitor etomoxir (both from Sigma-Aldrich), at 10 and 200μM respectively.
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bioRxiv - Systems Biology 2024Quote: ... morphine paradigm were communally housed (3-4 mice per cage) and had access only to water supplemented with either sodium butyrate (Sigma-Aldrich) or pH-matched (9.30 - 9.50 ...
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bioRxiv - Molecular Biology 2024Quote: ... N-(4-ethylphenyl)-2-[[4-ethyl-5-(3-pyridinyl)-4H-1,2,4-triazol-3-yl]thio}acetamide (ORco Receptor Agonist ORcoRAM2; OA) from Asinex Corporation and Vitas M Chemical Ltd; N,N-diethyl-3-methylbenzamide (DEET; V) from Sigma-Aldrich; and coelenterazine from Biosynth ...
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bioRxiv - Immunology 2021Quote: ... and Tumor necrosis factor-alpha (TNF-α)) were measured with a Milliplex MAP High-Sensitivity Human Cytokine Panel (HSCYTMAG-60SK-13, Millipore Corp., Billerica, MA) on a Luminex MagPix (Millipore Corp. ...