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Citations for Millipore Sigma :
9851 - 9900 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and the hACE2 stably expressing cell lines were selected and maintained with medium containing 3 μg/ml puromycin (Sigma, Missouri, USA). hACE2 expression was confirmed by immunofluorescence staining using mouse monoclonal antibody against c-Myc antibody 9E10 (Thermo-Fisher ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% (BC-1, BCBL-1) or 20% (BC-2, BC-3, BC-5, BJAB) Serum Plus-II (Sigma-Aldrich, catalog number 14009C ...
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bioRxiv - Cell Biology 2023Quote: ... and puromycin to select transduced cells (1 μg/ml for hNPCs; 3 μg/ml for SH-SY5Y cells; Sigma, Cat# P9620). Cells were passaged 4-5 times after lentiviral transduction to select for stable expression of shRNAs.
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bioRxiv - Cancer Biology 2023Quote: ... The resulting DNA was pipetted along one side of a coverslip that had been placed on top of a 3-aminopropyltriethoxysilane (Sigma-Aldrich)-coated glass slide and allowed to enter by capillary action ...
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bioRxiv - Microbiology 2023Quote: ... plated on fluorodishes were incubated in 50mM NH4Cl for 5min at room temperature (RT) to quench free aldehydes and in 1X PBS containing 3% Bovine Serum Albumin (BSA, Sigma-Aldrich) for 15min at RT to block non-specific epitopes ...
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bioRxiv - Biochemistry 2022Quote: Protease treatment of >30kDa FBS fraction was performed by treating 200 µL of large fraction (previously washed 3 times with 1X PBS) with 10 µL of 20 mg/mL Proteinase-K (#SAE0151, Sigma-Aldrich) at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: Acellular stretched and nonstretched hydrogels in 3 mg/mL and 6 mg/mL concentrations were created and fixed in 3.7% formaldehyde (Sigma-Aldrich 252549) in PBS at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: Precultures were grown in mineral media (Figure 3) or buffered SD media supplemented with 2000 µg/L thiamine (thiamine HCL, Sigma-Aldrich) (Figure 5 ...
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bioRxiv - Developmental Biology 2023Quote: ... the medium was changed to the same base medium with the addition of CHIR99021 (Chiron, 3 µM, Sigma-Aldrich SML1046-5MG), PD0325901 (1 μM ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue sample was trimmed to 2×3×0.2 mm3 and scanned with a multi-beam serial-section scanning electron microscope (Sigma, Carl Zeiss) in 4×4 nm2 pixels and 33 nm slice thickness ...
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bioRxiv - Cell Biology 2023Quote: ... 1% (w/v) BSA and 3% (v/v) serum of the same species as secondary antibodies were raised in (ordered from Sigma-Aldrich). Following blocking ...
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bioRxiv - Cancer Biology 2023Quote: ... IFI44L (#1: TRCN0000167757; #3: TRCN0000242541) or ISG15 (#4: TRCN0000007423)) in the presence of X-tremeGENE HP DNA Transfection Reagent (Sigma Aldrich). Five shRNAs per gene were tested for knockdown efficiency ...
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bioRxiv - Cancer Biology 2023Quote: Decellularized matrices were fixed for 30 minutes under gentle agitation (12 rpm orbital 154 shaker) in 3 % glutaraldehyde solution in cacodylate buffer (100 mM, Sigma 20840) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Brain slices were rinsed in PBS 3 times (5 min each) and then were stained with Hoechst (1:1000, #33342, Sigma-Aldrich) for 10 min ...
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bioRxiv - Physiology 2023Quote: ... Gene ablation was induced starting at 3 months of age using intra-peritoneal (20 mg/kg per day for 5 days; Sigma #T5648) and then chow-mediated intake (40 mg/kg ...
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bioRxiv - Neuroscience 2023Quote: ... a sense sequence based on 5’-GCCCTAATCCAGAATCTTGTA-3’ corresponding to nucleotides 2473-2493 of mouse mDia3 (DIAPH2; NM_172493.2; Sigma-Aldrich, Cat# TRCN0000108782) was used to construct f(U6 ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating brain sections were then incubated for 1 hour at 4C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... we added 50 μL of primary antibody mix (see Table 3) diluted in FACS buffer containing HBSS with 0.4% bovine serum albumin (Sigma-Aldrich, #A3983), 15mM HEPES (Gibco ...
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bioRxiv - Plant Biology 2023Quote: ... Flash-frozen plant samples (0.2 g) were ground and extracted using 2 mL of 3 % 5-sulfosalicylic acid (Sigma, ON, Canada). The extract was centrifuged for 10 min at 8050 x g at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with/without the oxidative agent H2O2 (250 μM for 3 h) or TBH (250 μM for 1 h) (tert-butyl hydroperoxide solution, Sigma-Aldrich) in the absence/presence of nanoalgosomes ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were blocked with 3% BSA on rotator for 30 min and washed with PBS and incubated with anti-Flag (Sigma, #F1804) or anti-HA antibody (Biolegend ...
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bioRxiv - Molecular Biology 2024Quote: ... cumulous-enclosed egg complexes were collected from the oviduct into Advanced KSOM media with hyaluronidase (3 mg/ml, Sigma Aldrich, H4272) for 2 min to remove cumulus cells ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was resuspended in buffers indicated in each analysis and homogenized by using a PTFE pestle in a 3 mL glass tube (Sigma, P7734). To further disrupt any remaining SV clusters before experiments ...
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bioRxiv - Microbiology 2024Quote: ... 150 µL x 3 from each sample was add to a well in Corning transparent 96-well plate (cat#CLS2585 from Millipore Sigma) and absorbance was measured at 690 nm in a Tecan Spark Plate Reader ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... slices were washed 3 times in cold phosphate buffer and postfixed with a solution of 2% osmium tetroxide (#75632 Sigma-Aldrich) and 1.5% potassium ferrocyanide (P3289 Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by the addition of 1 mg/mL MTT reagent (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma Aldrich, Darmstadt, Germany). After the incubation period of 4 h ...
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bioRxiv - Physiology 2024Quote: ... quail were placed in glass chambers (3.3 L at 3 weeks; 8.0 L at 8 weeks) ventilated with dried (via drierite; Sigma-Aldrich, Stockholm, Sweden) atmospheric air at least 30 minutes before measurement ...
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bioRxiv - Molecular Biology 2023Quote: ... Each recombinant protein was eluted twice in 250 µl of EB containing 1 mM DTT and 250 µg/ml 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... An anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2×SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... The cells were induced to differentiate using a 3 day treatment with 1% FBS media and 10 μM retinoic acid (Sigma R2625) followed by 4 days with zero FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were imaged under a microscope after the addition of an anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2xSSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
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bioRxiv - Microbiology 2023Quote: ... Protein knock-downs using the minimal Auxin-Inducible Degron (mAID) system were performed under 500 µM auxin (IAA: 3-indoleacetic acid in 100% ethanol; Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... These slices were then rinsed for 3 times in PBS (5 min each) and then stained nuclei with Hoechst (1:1000, #33342, Sigma-Aldrich) for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... Matrigel was first digested using 3 ml of dispase per well (PluriSTEM™ Dispase-II, 1 mg/mL, Millipore Sigma, SCM133) and incubated for 10 min in a 15 ml conical tube at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 15N isotopically labelled protein sample was made from a 1 L culture containing M9 minimal media salts supplemented with 3 g of 13C-glucose (Sigma-Aldrich), 1 g of 15N ammonium chloride (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (biotin-DOPE) (structures in Table S5) were purchased from Sigma-Aldrich (UK). Agarose powder was purchased from Invitrogen (UK) ...
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bioRxiv - Bioengineering 2023Quote: ... Spectral phasing was applied and spectra were aligned to 3-(trimethylsilyl)-1-propanesulfonic acid-d6 sodium salt (DSS-d6, Sigma-Aldrich) at 0 ppm ...
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bioRxiv - Cancer Biology 2023Quote: Dissected tumors were washed in PBS and homogenized at room temperature in urea lysis buffer (8 M urea, 40 mM Tris pH 7.6, 5% SDS supplemented with phosphatase inhibitor cocktails 2, 3 (Sigma P5726, P0044)) ...
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bioRxiv - Cell Biology 2023Quote: ... Biopsies were subsequently microtome sectioned to a thickness of 3 µm and mounted onto poly-L-lysine coated Poly-Prep glass slides (Cat. No. P0425-72EA, Sigma Aldrich). After incubating the slides at 37 °C over night deparaffination was performed ...
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bioRxiv - Immunology 2023Quote: ... in PBS for at least 30 min at 37 °C and permeabilized with PBS containing 0.1 % Triton-X for 5 minutes and blocked with blocking solution (3 % BSA in PBS) at RT for 30 minutes (all reagents from Sigma-Aldrich). Then ...
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bioRxiv - Microbiology 2023Quote: ... 50µL of NaCl were then added to the strip before being centrifuged (19,000 x g for 20 minutes, Sigma 3-16PK) to extract the tears ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then cultured for 3 h in T cell stimulation media (1000ng/mL PMA [Sigma Aldrich, Catalog no. P1585-1MG], 2µM Ionomycin [Sigma Aldrich, Catalog no ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 μl of the supernatant were transferred to a new tube and mixed with 3 volumes (900 μl) of Trizol LS (T3934, Sigma-Aldrich) by vortexing ...
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bioRxiv - Microbiology 2023Quote: ... 12 h post-transfection the media was removed and fresh media was added with 3 mM Sodium butyrate (Sigma-Aldrich, USA) to induce lentivirus replication ...
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bioRxiv - Cancer Biology 2023Quote: ... Myelin and debris removal was performed by a gradient centrifugation of the cell suspension in a 7:3 mix of PBS:Percoll (Sigma-Aldrich, #P4937), with an additional PBS wash ...
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bioRxiv - Systems Biology 2023Quote: ... 6 M Urea and 1% Sodium Dodecyl Sulphate supplemented with 100mM phenylmethylsulfonyl fluoride and 1% phosphatase inhibitor cocktail 3 (Sigma-Aldrich)) ...
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bioRxiv - Systems Biology 2023Quote: ... samples were dissolved in 50 μL water containing reference compounds [200 μM each of trimesate (Wako) and 3-aminopyrrolidine (Sigma-Aldrich)] ...
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bioRxiv - Evolutionary Biology 2023Quote: Total RNA was extracted from ∼150 injected animals (3 days old planulae) with the aid of Tri- Reagent® (Merck Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 1.7μM Acquity UPLC BEH C18 Column (Waters) with a gradient of solvent A (97:3 H2O:MeOH, 10mM TBA (Sigma Aldrich: 90781), 9mM acetic acid (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Degradation of EGFP-AID-STAG2 was induced by the addition of S00 µM Inole-3-acetic acid (IAA, ISl48, Sigma-Aldrich) for 2 hours at 37°C ...