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Citations for Millipore Sigma :
9751 - 9800 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... slides were blocked with 3% BSA on rotator for 30 min and washed with PBS and incubated with anti-Flag (Sigma, #F1804) or anti-HA antibody (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... 1.7μM Acquity UPLC BEH C18 Column (Waters) with a gradient of solvent A (97:3 H2O:MeOH, 10mM TBA (Sigma Aldrich: 90781), 9mM acetic acid (Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... slices were washed 3 times in cold phosphate buffer and postfixed with a solution of 2% osmium tetroxide (#75632 Sigma-Aldrich) and 1.5% potassium ferrocyanide (P3289 Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... 150 µL x 3 from each sample was add to a well in Corning transparent 96-well plate (cat#CLS2585 from Millipore Sigma) and absorbance was measured at 690 nm in a Tecan Spark Plate Reader ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was resuspended in buffers indicated in each analysis and homogenized by using a PTFE pestle in a 3 mL glass tube (Sigma, P7734). To further disrupt any remaining SV clusters before experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... cumulous-enclosed egg complexes were collected from the oviduct into Advanced KSOM media with hyaluronidase (3 mg/ml, Sigma Aldrich, H4272) for 2 min to remove cumulus cells ...
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bioRxiv - Systems Biology 2023Quote: ... samples were dissolved in 50 μL water containing reference compounds [200 μM each of trimesate (Wako) and 3-aminopyrrolidine (Sigma-Aldrich)] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by the addition of 1 mg/mL MTT reagent (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma Aldrich, Darmstadt, Germany). After the incubation period of 4 h ...
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bioRxiv - Physiology 2024Quote: ... and then transferred for 3 days into new tubes in the presence of food supplemented with 60 µM methoprene (Sigma-Aldrich, St Louis ...
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bioRxiv - Physiology 2024Quote: ... quail were placed in glass chambers (3.3 L at 3 weeks; 8.0 L at 8 weeks) ventilated with dried (via drierite; Sigma-Aldrich, Stockholm, Sweden) atmospheric air at least 30 minutes before measurement ...
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bioRxiv - Microbiology 2024Quote: ... and methyl N-[2-[[1-(4-chlorophenyl)pyrazol-3-yl]oxymethyl]phenyl]-N-methoxycarbamate (i. e. pyraclostrobin, pesticide analytical standard, CAS 175013-18-0, supplier: Sigma-Aldrich, purity ≥ 98.0% ...
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bioRxiv - Molecular Biology 2024Quote: ... containing a sgRNA sequence (5’-TCATCGCTCACAACCAAGTG-3’) targeting exon 7 of ERBB2 (cloned by Twin Helix) and selected with puromycin (Sigma-Aldrich) for 4 days (1 μg/ml puromycin for 72 hours ...
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bioRxiv - Microbiology 2024Quote: ... parasite pellets were placed on ice for 5 min before addition of 50 μl chloroform followed by 200 μl methanol:ultrapure water (3:1, including scyllo inositol as an internal standard, 1 nmol, Sigma-Aldrich, I8132). Extraction was facilitated through vigorous vortexing ...
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bioRxiv - Molecular Biology 2024Quote: In vivo expression of OSKM in all reprogrammable mouse strains was induced by continuous or 3-4 days administration of doxycycline (Sigma, D9891) in drinking water (1 mg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Microbiology 2024Quote: For the leukopenic model mice were immunosuppressed with 150 mg/kg of cyclophosphamide (Baxter) on days −3 and −1 plus 1 subcutaneous injection of 250 mg/kg hydrocortisone 21-Acetate (Sigma-Aldrich) on day −1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with PBS containing 0.1% (v/v) Tween-20 and either 5% (w/v) non-fat dry milk (Carnation) or 3% bovine serum albumin (BSA, Sigma-Aldrich) for phosphorylated proteins detection ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Bioengineering 2023Quote: ... at 10 μM or 1 mM from a 0.5 M stock solution of 3-MB (Sigma-Aldrich, St Louis, MO, USA) solubilized in 0.5 M NaOH ...
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bioRxiv - Plant Biology 2024Quote: ... a similar reaction was performed with both enzymes combined with 10 units phosphoglycerate kinase and 10 units glyceraldehyde-3-phosphate dehydrogenase (both Sigma-Aldrich), alongside 9 mM phospho-enol-pyruvate ...
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bioRxiv - Neuroscience 2024Quote: ... Cre-mediated recombination was induced in mice 3-7 months of age by intraperitoneal injection of 2 mg/day tamoxifen (Sigma-Aldrich) diluted with 300 μl corn oil (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: The viabilities of the PBMCs or U-937 macrophages were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Cell Biology 2024Quote: We assessed mitochondrial activity using the 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (Sigma-Aldrich, Cat# M6494). As a tetrazolium dye ...
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bioRxiv - Cell Biology 2024Quote: C57/BL6 mice at 9 months of age that were administrated with ABT263 or control vehicles at 3 months of age were injected with 5 IU of eCG (Sigma, G4877). The ovaries were collected at 44 hours after the eCG injection ...
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bioRxiv - Cell Biology 2024Quote: ... They were then IV injected via the common cardinal vein52 with 3 nl of PEVs along with loading buffer (sterile-filtered, endotoxin-free phenol red solution in DPBS; BioReagent, Sigma-Aldrich) giving a nominal dosage range of 105 - 106 particles per bolus estimated by NTA ...
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bioRxiv - Immunology 2024Quote: ... 100,000 to 200,000 T-allo10 cells were cultured in a 96-well plate for 6h in total of 200 uL of the base T cell medium (see Primary Cells section) with 3 ug/mL brefeldin A (Sigma Aldrich), 2 uM monensin (BD ...
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bioRxiv - Immunology 2024Quote: ... B cells were cultured in RPMI1640 medium supplemented with 10% (vol/vol) FBS and 1% penicillin-streptomycin and activated with 3 μg/mL LPS (Sigma-Aldrich), 10 ng/mL recombinant mouse IL-4 (Tonbo Bioscience ...
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bioRxiv - Biophysics 2023Quote: ... and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (biotin-DOPE) (structures in Table S5) were purchased from Sigma-Aldrich (UK). Agarose powder was purchased from Invitrogen (UK) ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed myotubes were blocked and permeabilized in 3% (m/m)-BSA solution with 0.1% (m/m) Digitonin (D5628, Sigma-Aldrich, Germany) in DPBS (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma) in washing solution for 1h ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs were seeded into the collagen gel channel with a media containing 3% dextran (Leuconostoc spp., Mr 450 000–650000, Sigma-Aldrich) at a density of 1 × 107 cells/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Culture supernatants were harvested at 3 days post-transfection and filtered through a 0.45 μm filter (Merck Millipore, Burlington, MA, USA). All proteins were purified with a Talon metal affinity resin (Takara Bio USA ...
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bioRxiv - Bioengineering 2024Quote: TEM grids functionalized with Strep-AuNPs were blocked with a 3 % (w/v) of bovine serum albumin (BSA) solution (Sigma-Aldrich) diluted in PBS for 15 min to reduce non-specific capture ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were seeded in 15-well Ibidi chambers after passaging and fixed 3-4 days later in 4% Paraformaldehyde (Sigma-Aldrich) for 60 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were seeded in 60 mm dishes at 50-60% confluency and transiently transfected with CEP41 shRNA (5’-GCTTACAGTTACCCAATTGCA-3’, TRCN0000143499, Sigma-Aldrich) or scramble shRNA (SHC002 ...
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bioRxiv - Cell Biology 2024Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS, 840035), and β-casein from bovine milk (>98% pure, C6905) were purchased from Sigma. BODIPY-TR-C5-ceramide ...
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bioRxiv - Cell Biology 2024Quote: ... GV oocytes were collected from the ovary of 3-4 weeks old C57BL/6 females 48 h after PMSG injection and then were incubated in M2 medium (Sigma, #M7167) supplemented with 10 μM Cilostamide (TargetMol ...
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bioRxiv - Cancer Biology 2024Quote: ... cytoplasmic (cytosolic + microsomal fractions (as described in “cell fractionation” of Methods section) cell extracts were digested with Benzonase (Millipore 70746-3) and precleared with protein-sepharose beads for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cytoplasmic (cytosolic + microsomal fractions (as described in “cell fractionation” of Methods section) cell extracts were digested with Benzonase (Millipore 70746-3) and DNase I (Thermo Fisher Scientific EN0521 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and enzymatically dissociated into a single-cell suspension in PBS supplemented with 3% fetal bovine serum (#FB-1101, Biosera) and 2 mM EDTA (#ED2SS, Sigma=:Aldrich). After 15 minutes of incubation with 5 nM SYTOX™ Red Dead Cell Stain (#S34859 ...
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bioRxiv - Cancer Biology 2023Quote: ... Myelin and debris removal was performed by a gradient centrifugation of the cell suspension in a 7:3 mix of PBS:Percoll (Sigma-Aldrich, #P4937), with an additional PBS wash ...
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bioRxiv - Immunology 2022Quote: ... a catheter was inserted and the infrarenal part was perfused with sterile isotonic saline containing type I porcine pancreatic elastase (2.5-3 U/ml Sigma Aldrich, #E1250) or 0.9% NaCl (sham surgery ...
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The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... Proteins were eluted by rotating the resin at room temperature for 1 hour in 100 μL of PBS containing 150 ng/ μL 3×-FLAG peptide (Sigma Aldrich). 100 μL of 2x sample loading buffer was added to the eluate and samples were boiled at 95 °C for 10 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 3 hours and then incubated overnight at 4°C in block with primary antibodies: mouse anti-acetylated tubulin (Sigma, T6793) at 1:250 or rabbit anti-phosphorylated Smad1/5/8 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... while another portion was concentrated (about 100X) and buffer exchanged to PBS using an Amicon stirred cell with a 3 kDa MWCO cellulose membrane (Sigma-Aldrich). The concentrated product contained T ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed with vDISCO washing solution for 3 hours and 3DISCO clearing60 with 50-70-90-100-100% tetrahydrofuran (THF) (Sigma, 186562), 1h each ...
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Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... Anhydrous 100% ethanol solution was prepared by adding 15 g of molecular sieve beads (Sigma 208582, 3 Å, 8-12 mesh) to 500 mL 100% molecular grade ethanol (Sigma E7023) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... diluted in corn oil (CO, used as vehicle) at 3 mg/kg for 4 days while CO mice received corn oil only (Sigma Aldrich). One cage of n=6 mice per group was used ...
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bioRxiv - Physiology 2023Quote: ... and then incubated overnight at 4°C in primary antibodies diluted in 50% blocking buffer / 50% Tris buffered saline (bioWORLD #42020056–3) + 0.1% TWEEN (Sigma-Aldrich #P9416) (TBS-T) ...
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bioRxiv - Molecular Biology 2023Quote: ... Freshly resected left ventricular tissue (~3-5 mg per sample) was pulled into fibers and permeabilized in the presence of saponin (50 μg/mL, Sigma, 47036) 30 min at 4°C in BIOPS buffer containing the following ...