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Citations for Millipore Sigma :
9551 - 9600 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... the medium was changed to N2B27 medium34 with 3 μM CHIR99021 (CHIR) and 10 μM SB431542 (SB) (both from Sigma-Aldrich) and cultured for 5 days ...
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bioRxiv - Cell Biology 2022Quote: ... Printed discs were incubated in 0.1 mg/mL FITC-labelled dextran molecules (size either 3-5 kDa, 10 kDa, 20 kDa or 70 kDa (Sigma-Aldrich)) dissolved in PBS with 20mM CaCl2 (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... they were cultivated 72 hours in the presence of 2 μM DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) (Sigma-Aldrich) to inhibit the synthesis of glucosylceramide-based GSLs 75 and incubated with fluorescent SaroL-1 as previously described ...
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bioRxiv - Biophysics 2022Quote: ... Cover glasses were then activated by silanization with an ethanol solution containing 1% v/v (3-Aminopropyl) trimethoxysilane (Sigma Aldrich, 281778) for 15 minutes ...
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bioRxiv - Biophysics 2022Quote: ... Selected fractions (shown in Figure S4) were pooled and concentrated using Spin filters (3 kDa molecular weight cutoff, Amicon, Millipore Sigma) and stored at 4 °C before downstream characterizations ...
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bioRxiv - Biophysics 2022Quote: ... except that the cells were washed and incubated with 3 μM BioTracker™ 609 Red Ca2+ AM Dye (EMD Millipore #SCT021) in PBS for 30 min in a tissue culture incubator ...
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bioRxiv - Bioengineering 2022Quote: ... The retinal strips were sectioned (7–10 µm thick) and placed on microscope slides that were treated with 3-Aminopropyltriethoxysilane (APES, Sigma A3648). Before immunohistochemistry ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were permeabilized for one hour in a solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503). Sections were incubated with primary antibodies in a solution of 1.5 % BSA and 0.2 % Triton X-100 overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were passaged and one of the following antibiotics were added to the medium in order to select for the cells which stably express the respective construct: Puromycin dihydrochloride (3 µg/ml, Sigma Aldrich), blasticidine S hydrochloride (15 µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transduced for 1-3 consecutive days with the viral stock diluted 1:3 with culture media in the presence (or absence for BT-549 cell line) of 8 µg/mL polybrene (Sigma-Aldrich). The viral solution was replaced with fresh culture medium ...
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bioRxiv - Cell Biology 2022Quote: The neuroprotective effect of CA against 6-OHDA was evaluated by MTT reagent (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) (Sigma Aldrich, UK, M5655) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2021Quote: ... seedlings were transferred for another 3 days in Hoagland containing poorly available Fe and 100 µM sodium orthovanadate or 150 µM glibenclamide (both from Sigma-Aldrich). For agar plates ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating VTA brain sections were incubated 1h at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% Bovine Serum Albumin (BSA, Sigma A4503) and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH ...
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bioRxiv - Plant Biology 2021Quote: ... Lipids extracts were incubated 1 h at 50°C in 2 mL of methylamine solution (7 ml methylamine 33% (w/v) in EtOH combined with 3 mL of methylamine 40% (w/v) in water (Sigma Aldrich) in order to hydrolyze phospholipids ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were washed 3 times with PBS and mounted on a microscope slide with parafilm gasket in 1% (v/v) β-mercaptoethanol (Sigma), 150 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... were seeded for 72 h on polycarbonate membrane Transwell permeable inserts (Costar, 3 μm pore size, 12 mm diameter, Sigma Ireland) and incubated for three days ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated testicular cells in PBS were fixed with PFA in 4% concentration for 5 minutes followed by centrifugation at 250 x g for 3 minutes to attach to coverslips coated with poly-D-lysine (Sigma Aldrich). Testicular cells were air-dried shortly ...
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bioRxiv - Cell Biology 2021Quote: ... Media was replaced under the microscope after 3 hours with fresh L15 media containing 10% FBS with a mock solution (1/1000 of DMSO - Sigma D8418), 10μM Nutlin-3 (Sigma N6287) ...
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bioRxiv - Microbiology 2020Quote: Infected cells grown on coverslips were fixed with 4% paraformaldehyde and permeabilized/blocked with a Triton-X100 (0.2%)/BSA (3%) solution and then incubated with mouse anti-Flavivirus Group Antigen 4G2 (Millipore, Burlington, MA), mouse anti-alphavirus capsid (kindly provided by Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified in 40 μL reactions with 15-18 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and 500 nM of each primer P1.3 and P2.1 (synthesized by IDT ...
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bioRxiv - Molecular Biology 2020Quote: ... 4OHT treated cells were washed 3 times with pre-warmed PBS and further incubated with 500 µg/mL auxin (Sigma; I5148). For transcriptional inhibition ...
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bioRxiv - Biophysics 2021Quote: ... for 1 hour at room temperature and washed 3 times with PBS-T before mounting in 1% low melting point agarose (T9284, Sigma-Aldrich) followed by light sheet imaging ...
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bioRxiv - Neuroscience 2020Quote: Fluorescent immunostaining for nuclear NFκB and cytoplasmic NFκB was quantified in immortalized C8D30 astrocytes following 2-hr treatment with 1μg/ml LPS or a cocktail of 3 cytokines: Il-1α (3ng/ml, Sigma, Cat# I3901), TNFα (30ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... few selected free floating sections corresponding to the Somatosensory cortex were mounted on glass using chromium (3) potassium sulfate dodecahydrate (Sigma-Aldrich) and left to dry overnight ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells from iCD4-PRMT5fl/fl and iCD4-PRMT5Δ/Δ mice (n = 3 pooled mice/sample and n = 3 samples/group) were lysed in our in-house lysis buffer (50 mM triethylammonium bicarbonate, Millipore Sigma, catalog no ...
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bioRxiv - Immunology 2020Quote: ... To activate the NLRP3 inflammasome BMMs were first primed with LPS in cell culture medium or left unstimulated for 3 h before cells were treated with 5 µM nigericin (Sigma-Aldrich) for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then treated for 15 min up to 4 h at 37°C with 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, #M5655-1G) in 5 mM PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with TBS-T (3 times for 10 min) and incubated with horseradish peroxidase-conjugated secondary antibodies anti-mouse (Millipore, #AP308P), or anti-rabbit (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The uncoupled maximal capacity of the electron transport system (ETS) was obtained after titration with 0.5 μM of uncoupler carbonyl cyanide 3-chlorophenylhydrazone (CCCP, Sigma Aldrich, C2759) up to the complete dissipation of the proton gradient ...
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bioRxiv - Neuroscience 2021Quote: ... the reporter cells were exposed to drugs or DMSO vehicle for one hour before the addition of 3 ng/mL IL-1a (Sigma, I3901), 400 ng/mL C1q (MyBioSource ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-free DMEM for 2 hrs before a 3-hr incubation in glucose-free or low-glucose DMEM/fatty acid-free BSA (Sigma-Aldrich) 0.2% supplemented with a mixture of cold and radiolabeled palmitate (Palmitic Acid ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970; 1:200 rabbit anti-cleaved caspase 3, EMD Millipore, AB3623). Sections were washed several times in TBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stained for DAPI diluted in water for 2 min and washed 3 times with water before drying cells on poly-L-lysine (Sigma, P8920) coated coverslips at room temperature protected from light ...
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bioRxiv - Microbiology 2021Quote: Calu-3 barrier integrity was determined by trans-epithelial electrical resistance (TEER) measured with a Millicell-ERS2 Volt-Ohm meter (Millipore Sigma). Barrier integrity was further assessed using the Human E-cadherin Quantikine enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Biophysics 2021Quote: ... the cells were washed 3 times with 500 µl of PBS and then were fixed and permeabilized with ice-cold 100% methanol (Sigma Aldrich) for 15 minutes at -20°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and the pellet resuspended and incubated for 3-7 minutes at 37°C in accutase dissociation reagent (Sigma, Poole, UK, A6964). After enzyme neutralization with fresh medium ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue samples from brain and peripheral organs were homogenized in 3:1 (v/w) of PBS containing 1% Triton X-100 supplemented with protease inhibitors (cOmplete™, Sigma) using the TissueRuptor (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Cancer Biology 2022Quote: ... Mice were induced to form tumors at 2-3 months of age by administration of tamoxifen (Sigma-Aldrich, St. Louis, MO) using 100 mg/kg (in corn oil ...
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bioRxiv - Microbiology 2022Quote: ... Tissue sections were then permeabilized overnight at 4°C by gently rocking in blocking buffer comprising 3% bovine serum albumin (Sigma Aldrich), 1% saponin (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 10 µl of hemolymph was mixed with 90 µl of 3, 4-Dihydroxy-L-phenylalanine (L-DOPA, 4 mg/ml)(Sigma Aldrich) dissolved in nuclease free water (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was spun at 1000g for 3 minutes and re-suspended in islet media-G11 mixed with concentrated lentivirus and DEAE-Dextran hydrochloride (Sigma Millipore). The mixture was loaded to the GravityPLUS hanging drop 96-well microplate (InSphero ...
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant protein was eluted twice in 250 μL EB containing 1 mM DTT and 250 μg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Pathology 2022Quote: ... at room temperature for 10 minutes and washed 3 times in PBS containing 0.1 % v/v Triton X-100 (Sigma-Aldrich X100), blocked for 30 min using 10 % v/v chicken serum (Gibco 16110082 ...
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bioRxiv - Neuroscience 2022Quote: ... with nerves from 3 mice pooled per time point in ice-cold PBS with 1% protease inhibitor cocktail (Sigma-Aldrich, P8340). Samples were minced ...
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bioRxiv - Bioengineering 2022Quote: ... Bone-marrow derived hMSCs (hBMSCs) were frozen at passage 3 or 4 with 5*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Bioengineering 2022Quote: ... the 11-mercaptoundecanoic acid (CAS no: 71310-21-9) and L-Tryptophan (CAS no: 73-22-3) were obtained from Sigma Aldrich. The RBD ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed two more times with PBS (Biotech, PD8117) then blocked and permeabilised using a 3% BSA (Sigma-Aldrich, 1073508600), 0.2% Triton X-100 (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
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bioRxiv - Molecular Biology 2019Quote: ... HeLa cells were treated with 60 μM of the adenosine analogue 5,6-dichloro-1-3-D-ribofuranosylbenzimidazole (DRB; Sigma-Aldrich®) to inhibit transcription and RNA was extracted at different time points for further RT-qPCR analysis.