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Citations for Millipore Sigma :
9351 - 9400 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an ChemidocTM MP (Biorad ...
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bioRxiv - Molecular Biology 2024Quote: ... either 200-300 μg of cleared protein extract lysed in RIPA buffer was incubated with anti-FLAG M2 Magnetic Beads (Sigma) for 1 hour at 4 °C with rotation followed by 3 washes with RIPA buffer or 200-300 μg or protein extract was incubated with 2-5 μg of Y69 N-terminal c-MYC ...
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bioRxiv - Molecular Biology 2024Quote: ... For shRNA-mediated knockdown, all TRC pLKO.1 vectors (targeting the YTH domain-containing proteins, THOC1, and THOC2) were purchased from Sigma. The TRIPZ doxycycline (Dox)-inducible lentiviral YTHDC1 shRNA vector is purchased from Dharmacon.
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bioRxiv - Genomics 2024Quote: ... 1 mL of the supernatant was filtered through a syringe using an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic Millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland).
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bioRxiv - Neuroscience 2024Quote: ... the bound protein was visualized using 100 µl of Tetra-methyl-benzidine liquid substrate (TMB slow kinetic form, Sigma-Aldrich) at RT for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with primary antibodies against protein of interest (NPR, Santa Cruz, sc-39008, 1:100; GFP, Aveslab, GFP-1020, 1:5,000; FLAG, Sigma, F1804 ...
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bioRxiv - Neuroscience 2024Quote: ... The protein sample after elution was collected and concentrated to 2 mg/mL using a 50kDa molecular weight cutoff concentrator (Millipore).
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bioRxiv - Cancer Biology 2024Quote: ... Primary emerin (Protein-tech, cat# 10351-1-AP, 1:3,000 dilution) and γ-tubulin (Sigma, cat# T6557, 1:25,000 dilution) antibodies were incubated overnight with rocking at 4°C and secondary antibodies (goat anti-rabbit [cat# 31462 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
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bioRxiv - Microbiology 2024Quote: Gene fragments encompassing the employed proteins and their variants were amplified by polymerase chain reaction and inserted into a pET24d vector (Novagen) via NcoI/XhoI restriction sites ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant proteins were resolved on a 10% SDS-PAGE and transferred to a nitrocellulose membrane (0.45 μm, Millipore, USA). Membranes were blocked in blocking buffer (5% nonfat milk ...
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bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44, Sigma) with an NGC Chromatography system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... 25-30µg protein was loaded on resolving 12% SDS-PAGE and later transferred to a methanol-activated PVDF membrane (Millipore-# IPVH00010) by using a semi-dry Trans blot apparatus (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated by electrophoresis on precast gradient SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore) using a wet transfer tank (Hoefer Instruments) ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Cell Biology 2023Quote: ... Protein was transferred to a PVDF membrane 0.2 μm (Amersham) and blocked in 5% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 20 min ...
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bioRxiv - Genetics 2023Quote: ... Samples were then further purified to remove protein by filtration at 14,000 rpm for 30 minutes through Amicon® Ultra-0.5 spin columns (Sigma Aldrich) which have a 30 kDa molecular weight cutoff62 ...
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bioRxiv - Developmental Biology 2024Quote: ... The protein lysates were electrophoretically separated on sodium dodecyl sulfate-polyacrylamide gels and electrically transferred to polyvinylidene fluoride membranes (IPVH00010, Millipore). The membranes were blocked in 5% skimmed milk for 1 hour and incubated with the primary antibodies (Table S6 ...
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bioRxiv - Immunology 2024Quote: WB analysis of protein levels was performed on whole-cell lysates prepared by lysis in radioimmunoprecipitation assay buffer (Sigma-Aldrich) supplemented with Benzonase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of cell culture was harvested by centrifugation and lysed by incubation for 20 min with 200 µl in BugBuster protein extraction reagent supplemented with 1uL of Benzonase nuclease (Millipore), 0,6 µl of lysozyme (10 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Eluted protein complexes were concentrated to 400 μL in an Amicon® Ultra-15 Centrifugal Filter Unit (Millipore, Burlington, MA) and further subjected to a size exclusion chromatography through a Superdex 200 Increase 10/300 column (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Cell Biology 2024Quote: ... equal amounts of protein samples were loaded onto SDS–PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) which were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Genetics 2024Quote: ... Protein concentration of lysates was determined and 70 µg protein was loaded onto a 10% SDS-PAGE and subsequently transferred to a PVDF-FL membrane (Millipore). Membranes were incubated overnight at 4°C in primary antibodies (Flag ...
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bioRxiv - Immunology 2024Quote: ... Each 1 ml collected fraction was analyzed for protein concentration using a Nanodrop spectrophotometer (absorbance at 280 nm) and pooled three fractions were further analyzed for protein concentration using Bradford assay (Sigma) and used to measure biological activity to induce Arg1 expression in RAW264.7 macrophage cell line ...
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bioRxiv - Immunology 2024Quote: Activated TKO CD4+ T cells were activated with Cell stimulation cocktail plus protein transport inhibitors (eBioscience) and PMA (500 ng/ml, Sigma) for 4 hrs and then incubated with antibodies targeting cell surface markers ...
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bioRxiv - Immunology 2024Quote: ... Cytoplasmic protein extracts were obtained by incubation of cells with lysis buffer [50 mM NaH2PO4 (Sigma-Aldrich, Cat. No. S8282), 200 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Each target protein was buffered and concentrated in 5 mM MOPS (pH 7.0) by ultrafiltration with AmiconUltra (Millipore, MA, USA) or VivaSpin (Sartorius ...
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bioRxiv - Biophysics 2024Quote: ... and the supernatant containing the solubilized protein was collected for affinity purification by batch-binding to 10 mL of M2-agarose FLAG resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... 15N] triple-labeled proteins for NMR assignments at pH 5.0 were expressed in M9 minimal medium prepared using 100% D2O (Sigma-Aldrich) as solvent (M9 D2O medium) ...
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bioRxiv - Biochemistry 2024Quote: ... Imidazole was removed from the purified protein by serial buffer exchange in 10 kDa MWCO spin columns (Millipore Sigma, UFC801096). For CD44-Fc constructs ...
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bioRxiv - Biochemistry 2024Quote: ... Each sgRNA (30 ng/ul) was complexed with enhanced specificity (eSP) Cas9 protein (50 ng/ul from Millipore-Sigma (58) and individually validated for causing chromosome breaks in mouse zygotes ...
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bioRxiv - Biochemistry 2024Quote: ... Each sgRNA (30 ng/ul) was complexed with enhanced specificity (eSP) Cas9 protein (50 ng/ul from Millipore-Sigma (58) and individually validated for causing chromosome breaks in mouse zygotes ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were subjected to SDS-polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Sigma-Aldrich). Membranes were blocked with 5% milk in TBST for 1 h and incubated with antibodies as listed in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... The hexahistidine-tagged protein was expressed in Hi5 insect cells (Trichoplusia ni) grown in 1 liter of M3 medium (Sigma) supplemented with 10% fetal bovine serum (Wisent) ...
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bioRxiv - Biophysics 2024Quote: ... Buffer exchange to 1 x PBS was performed using desalting column (Generon) and eluted protein was concentrated with 30kDa MWCO Amicon (Millipore) to 20 mg/mL ...
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bioRxiv - Biophysics 2024Quote: The purified protein was concentrated to 5–8 mg/ml using a 50 kDa molecular weight cutoff (MWCO) centrifuge concentrator (Millipore) and loaded in 0.5 mL portions onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... ShOpa1 transduced cells were lysed and 35 μg of protein was electroblotted to PVDF membranes (EMD Millipore, Burlington, MA, USA). The membranes were blocked and incubated overnight at 4°C with antibodies against anti-BNIP3 (3769) ...
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bioRxiv - Cell Biology 2024Quote: ... Extracted proteins were resolved on a 10% SDS-PAGE gel and electroblotted onto a PVDF membrane (Sigma Aldrich, Cat # IPVH00005). The membranes were blocked with 1X BlockerTM FL 10X Fluorescent blocking buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 600-800μg of protein were diluted in 200μl and added to 50μl of anti-FLAG® M2 affinity gel slurry (Millipore, A2220). Samples were incubated for 1 hour rotating at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were loaded onto SDS-PAGE gels and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA) which were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation and lysed by incubation for 20 min in BugBuster protein extraction reagent supplemented with Benzonase nuclease (Millipore) and an EDTA free protease inhibitor cocktail (Roche) ...
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bioRxiv - Cell Biology 2023Quote: Approximately 100 µg protein of each sample was separated by 12% SDS-PAGE and transferred to an Immobilon-PVDF membrane (Merck Millipore). The blot was blocked with 5% non-fat milk in 1× TBST (20 mM Tris base ...
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bioRxiv - Biochemistry 2023Quote: ... N-glycomics of BLCA cell lines was performed similarly after immobilization of membrane protein extracts in a hydrophobic PVDF membrane (MAIPS4510; Millipore). All glycans were permethylated and analysed by reverse phase nanoLC- ESI-MS/MS as previously described by us69 ...
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bioRxiv - Biochemistry 2024Quote: ... The nhTMEM16 protein peak was collected and concentrated using a 100 kDa molecular weight cut off concentrator (Amicon Ultra, Millipore). The N-terminal GFP-His10 tag was cleaved overnight with 3C protease before gel filtration chromatography by using a Sepharose 6 Increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Bioengineering 2024Quote: Proteins were isolated from 1E6 cells in complete RIPA buffer with protease and phosphatase inhibitors (Sigma-Aldrich, St. Louis, MO). Total protein was quantified using the Pierce BCA Protein Assay Kit (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... The final protein was concentrated to ∼10 mg ml-1 using a 30 kDa molecular weight cut-off concentrator (Amicon Ultra, Millipore). The concentrated protein was flash-frozen and stored at −80 °C until further use.