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Citations for Millipore Sigma :
9301 - 9350 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: MSC derived small EVs were characterized for protein concentration using the bicinchoninic acid (BCA) assay (Sigma Aldrich, Oakville, ON, Canada). Nanoparticle tracking analysis was performed using ZetaView (Particle Metrix ...
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bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: The nitrocellulose membrane was imaged for total protein and then blocked for one hour in blocking buffer (5% w/v bovine serum albumin [Sigma, SKU A7906] in TBS-T buffer [50mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... Lysates were assayed for phospho proteins using the Milliplex Cell Signaling kit (NFκB Magnetic Bead Signaling 6-plex, Millipore Sigma) for detection of NFκB pSer536 ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Genetics 2022Quote: ... then the rest was split into two tubes with addition of 30 μl prewashed Protein A Agarose beads (Sigma, 11134515001), 3 μg anti-RAD51 (PHYTOAB ...
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bioRxiv - Genetics 2022Quote: ... Equal amounts of protein (40 µg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto Immobilon-P membranes (Millipore), as previously described (Nakamori et al. ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: ... About 2×106 cells of HEK293T cells were transfected with 2 ug of plasmids encoding S proteins using polyetherimide (PEI; Sigma). After 16 hours of incubation ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were concentrated by centrifugation using spin columns (MW cut off of 10 kDa) (cat number UFC501096– Merck Millipore). Protein concentration was estimated by UV using calculated extinction coefficients (42,400 mol/l-1.cm-1) ...
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bioRxiv - Microbiology 2022Quote: ... or drug free tissue homogenate and extraction was performed by adding 200 µL of acetonitrile/methanol 50/50 protein precipitation solvent containing 10 ng/mL Verapamil (Sigma). Extracts were vortexed for 5 minutes and centrifuged at 4000 RPM for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were separated in a 10% SDS-polyacrylamide gel and transferred to Immobilon NC membranes (Millipore Merck, Darmstadt, Germany). The membranes were blocked with 5% non-fat dry milk in PBS for 1 h at room temperature and then incubated with primary antibodies (DB 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... The polyclonal anti-Hcp antibody was developed after immunization of six-week-old female BALB/c mice with three subcutaneous injections of the purified His-Hcp protein emulsified in Freund adjuvant (Sigma).
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bioRxiv - Molecular Biology 2022Quote: Recombinant proteins used for biochemical assays were expressed from pMal-RT plasmids (see above and Table S3. For each protein preparation, E. coli Rossetta2 CapR cells (Novagen) containing freshly transformed expression constructs were plated on LB agar containing ampicillin (100 µg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Sizes were only referenced according to the Gel Filtration Makers Kit (for protein molecular weight 29 kDa to 700 kDa, Sigma).
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bioRxiv - Neuroscience 2022Quote: ... using rabbit antisera to the microglial protein Iba1 (1:1000; Fujifilm Wako Pure Chemical Corp) and AlexaFluor Donkey anti-rabbit 594 (1:1000; Millipore). Slides were cover slipped with Vectashield containing DAPI (Vector laboratories).
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bioRxiv - Pathology 2022Quote: ... Fifty microgram of protein was separated by SDS electrophoresis using 10% polyacrylamide gel and transferred to an Immobilon PVDF membrane (Millipore). Membrane was blocked with 5% non-fat milk in TBST for 2 hrs and probed overnight at 4°C with the primary antibody against TIM-1/KIM-1/HAVCR (#AF1817 ...
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bioRxiv - Plant Biology 2022Quote: ... SDS-PAGE/Western blot analysis was used to identify proteins in the sample with use of anti-FLAG M2 antibody (Sigma) and anti-MYC antibody (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... and the double phosphorylated form of S6 ribosomal protein (p-Ser240-244 -S6rp) were simultaneously detected through incubation with combined polyclonal goat anti-ChAT (1:500, #AB144P, Millipore) and polyclonal rabbit anti-p-Ser240-244 -S6rp (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Thirty μg of protein sample was run at 120V to separate proteins based on their molecular weights before electrophoretic transfer onto Polyvinylidene Difluoride (PVDF) transfer membranes (Millipore). PVDF membranes containing separated protein samples were blocked for one hour to prevent non-specific primary antibody binding using a solution of 5% skimmed milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, #17-700) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated on 10% SDS-PAGE and (semi-dry) blotted onto Polyvinylidene fluoride (Immobilon-P™, Millipore®) membranes.
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bioRxiv - Plant Biology 2023Quote: ... 1.4 ml of the remaining filtered total protein extract was mixed with 30 μl of anti-c-Myc agarose beads (A7470, SIGMA) and incubated end over end for 90 minutes at 4 ºC ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were separated on a 10% SDS-PAGE gel and Western blotting was performed using anti-FLAG M2 (Sigma F3165) and anti-GAPDH (Sigma A9521).
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were diluted to equivalent volumes containing 20 μg of protein and boiled in an equal volume of Laemli SDS boiling buffer (Sigma) for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (40 μg) were separated by 4-10% SDS-PAGE and transferred to a PVDF membrane (Immobilon-P, Millipore). Membrane was stained with anti-OXPHOS (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads-antibody-protein mix was incubated overnight at 4°C and bounds protein were eluted with 35 μg HA peptides diluted in PBS (Sigma); then Laemmli buffer was added and the eluate heated at 95°C 2 min.
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bioRxiv - Molecular Biology 2022Quote: ... the eluted protein is pooled and concentrated to ∼40 mg/mL by using a 50 kDa cutoff ultrafiltration unit (Millipore). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein pellets were resuspended in 10 μl of H2O and 0.3 μl of Benzonase (≥250 units/μl, Sigma, E1014)/sample and the samples were incubated in RT for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... The first elution peak containing both proteins were pooled and concentrated to 10-15 mg/ml using Amicon Ultra Centrifugal Filter Units (Millipore) with 10-kDa molecular weight cutoffs.
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bioRxiv - Plant Biology 2022Quote: ... 1mL of the supernatant was filtered through a syringe with an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland). The KTI in solution was separated on an Agilent 1260 Infinity series (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Golgi apparatus was detected with mouse anti-Golgi 58K protein /formiminotransferase cyclodeaminase (FTCD) antibody (G2404, SIGMA-Aldrich, Prague, Czech Republic). The following secondary antibodies were used for detection ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μl of the soluble protein sample were mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich, cat# UPS2) diluted in 250 μl 1.25× protein loading buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The protein was quantified according to the Coomassie blue method using bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA) as a standard (Bradford 1976) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The purity of the proteins was examined by SDS-PAGE and the concentration was measured with Bradford reagent (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNPs were produced by complexing a two-component gRNA (Edit-R, Dharmacon Horizon) to Cas9 protein with the addition of a PGA (Sigma) electroporation enhancer ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amount of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Membranes were blocked for one hour in I-BlockTM (# T2015 ...
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bioRxiv - Plant Biology 2024Quote: ... Total proteins were extracted using denaturing buffer (100 mM Tris-HCl pH 7.5, 4% SDS (sodium dodecyl sulfate, Sigma-Aldrich), 20% glycerol ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylation of NopTC93S proteins was analyzed by 50μM Zn2+-Phos-tag SDS-PAGE and detected with anti–FLAG (Sigma, F1804) antibodies by immunoblot analysis.
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bioRxiv - Microbiology 2024Quote: ... A total of 1 μg MBP-fusion protein (in 100 μl of 1% NP40) was incubated with 0.5 μg Avicel microcrystalline cellulose (Sigma-Aldrich) for 3 hours at 4°C with rocking ...
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bioRxiv - Bioengineering 2024Quote: ... and secreted proteins in media were harvested after 120 hours and cleared using a 0.45-µm PVDF vacuum filter (Sigma Millipore). Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed as previously described7 using Protein G Dynabeads (LifeTechnologies) coupled to Myc 4A6 antibody (Merck Millipore, 05-724) or FLAG M2 monoclonal antibody (Sigma ...
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... The protein extracts (50 µg) were then in-gel digested with trypsin overnight (1:100, w/w, Sigma-Aldrich, USA). Peptides were applied to a reverse-phase liquid chromatographer [Acquity UPLC M-Class System (Waters Corporation ...
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bioRxiv - Microbiology 2024Quote: ... Proteins in the lysate were then fractionated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, Cat#IPVH00010). After blocking with 1% nonfat milk overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Full length IMP2 protein was expressed as a pET-45 6-Histidine tagged 3C protease cleavable protein in Rosetta (DE3) pLysS Escherichia coli (Novagen) cells at 18 °C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an ChemidocTM MP (Biorad ...