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Citations for Millipore Sigma :
9251 - 9300 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... contaminant protein YadF was co-purified with the production of Arabidopsis Metacaspase 4 (AtMC4) in BL21 pLysS (DE3) cells (Novagen). Cells were lysed using a homogenizer and the soluble fraction of AtMC4 was collected for a three-step purification by nickel-nitrilotriacetic acid (Ni-NTA ...
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bioRxiv - Microbiology 2021Quote: ... Each animal received a single dose of 20 μg protein immunogen in 100 μl of PBS containing 50 μl of Sigma Adjuvant System (Sigma) with the procedure of immunogen and adjuvant mixture following manufacture’s manual ...
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration of cell lysates was measured using Bradford assay and equal amount of protein was added to anti-flag M2 magnetic beads (Sigma) in the presence of RNaseA (50 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... The resin was washed with binding buffer and the protein was eluted with binding buffer supplemented with 10 mM reduced glutathione (GSH, Sigma). Elution fractions containing the protein of interest were concentrated using a centrifugal filter (Amicon Ultra ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The diet was made with a fixed total amount of nutrients of 170 g/L (protein + carbohydrates) and 0.5% of vitamin mixture for insects (Sigma, Germany). Carbohydrates were supplied as sucrose (Euromedex ...
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bioRxiv - Genetics 2021Quote: ... glial fibrillary acidic protein (GFAP; polyclonal 1:1000, ZO334, DAKO-Agilent, Santa Clara, CA, USA; monoclonal 1:1000, MAB 3402, Millipore, Burlington ...
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bioRxiv - Neuroscience 2021Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore #17-700) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... irradiated 18% protein rodent diet (Teklad Global) alone (control) or supplemented with 0.2% w/w bis(cyclohexanone) oxaldihydrazone (Cuprizone, Sigma-Aldrich) in gravity-fed food dispensers for three weeks ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Plant Biology 2021Quote: ... Then the proteins were concentrated to 10 mg/ml using the 30 kDa cut-off Amicon Ultra-4 centrifugal filter unit (Millipore), and stored at −80 °C for crystallization.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged at 10,000 g for 5 minutes and total protein in the supernatant was measured using the bicinchoninic acid (BCA) assay (Sigma). Primary antibodies were detected using a secondary antibody conjugated to horseradish peroxidase ...
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bioRxiv - Cell Biology 2020Quote: ... were washed with 100 μl worm protein lysis buffer and incubated with monoclonal M2 mouse anti-Flag antibody (Sigma, F3165) or monoclonal rabbit anti-c-Myc antibody (Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Citrulline-containing proteins were modified on the membrane and detected using the anti-modified citrulline detection kit (Millipore, 17-347) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... 700 µg of protein was subjected to immunoprecipitation using 30 µl of Anti-FLAG M2 affinity beads (A2220, Sigma-Aldrich). The beads were incubated with rotation for 2 h ...
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bioRxiv - Genomics 2021Quote: ... Equal amounts of protein were separated by 10% SDS-PAGE gel and transferred onto a polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were incubated with RCC2 (CST ...
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bioRxiv - Immunology 2020Quote: ... M and N proteins were concentrated and buffer changed to phosphate buffered saline (PBS) using Microcon-10 KDa centrifugal filter units (Millipore). For N-terminal (residues from 43 to 180 ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... and concentrated using Amicon concentrator devices (molecular weight cut-off, 10 000 and for full-length protein 50 000; Merck Millipore). Recombinant His-tagged proteins (MA4376 ...
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bioRxiv - Immunology 2020Quote: ... Each region of the protein was amplified via PCR and fragments were combined using Gibson assembly into pET28 vector (Millipore) for expression in E ...
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bioRxiv - Immunology 2021Quote: ... Purified immunoglobulin (Ig) was purified using an antibody purification system based on protein A-affinity chromatography (PURE-1A; Sigma-Aldrich). The manufacturer’s instructions were as described in [8] ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 or MERS-CoV RBD protein was exchanged into 0.01 M PBS [pH 7.2] buffer using centrifugal filter units (10k MWCO; Millipore Amicon Ultra). A 5-fold molar excess of SM(PEG)24 (PEGylated ...
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bioRxiv - Immunology 2021Quote: ... Cells were separated by centrifugation at 6,000 x g for 5 min and cleaved proteins in the supernatant were concentrated and dialyzed with cleavage buffer using an Amicon filter of 10kD MWCO (Millipore) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 0.5 mL of SARS-CoV-2 S-2P protein was mixed with either an equal volume of Sigma Adjuvant System S6322 (Sigma), 600 μg/mL Adju-Phos aluminum phosphate (Brenntag) ...
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bioRxiv - Microbiology 2021Quote: ... Total histones were separated by 15% SDS-PAGE gel and protein blots were detected with anti-H3 (Millipore, 06-755), anti-H3K27me3 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... The purified protein was concentrated to about 5-10 mg/ml using Amicon concentrators with 30K MWCO (Millipore, Etobicoke, ON), and exchanged into the desired buffer ...
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bioRxiv - Microbiology 2020Quote: ... bLf solution was sterilized by filtration using 0.2 μm Millex HV at low protein retention (Millipore Corp., Bedford, MA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... and the activity of Rac1 and Cdc42 were assessed by affinity purification using magnetic beads coupled to the p21-binding domain of p21-activated protein kinase (PAK) (Millipore) that specifically binds the active (GTP-bound ...
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bioRxiv - Immunology 2021Quote: ... Fractions containing the proteins were pooled and concentrated to ~ 8 mg/mL each with 30 kDa cutoff ultrafiltration units (Millipore). Time-resolved fluorescence resonance energy transfer (TR-FRET ...
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bioRxiv - Microbiology 2021Quote: ... protein concentration was determined at A260 and equal quantities of protein from different samples were mixed with Anti-FLAG M2 magnetic beads (Sigma), washed with 20 bed volumes to remove non-specifically bound proteins and eluted using 0.1M glycine ...
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bioRxiv - Microbiology 2021Quote: Samples containing equal amounts of protein were separated using sodium dodecyl sulfate (SDS)-PAGE and then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore). After blocking with 5% skim milk in TBST for 2 h ...
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bioRxiv - Immunology 2020Quote: ... The protein was eluted with PBS containing 300 mM imidazole and 0.1% (v/v) protease inhibitor cocktail (Sigma, P-8849). For the CR3022 antibody ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated proteins were collected using 15 min centrifugation at 1700rcf in an Amicon® Ultra-4 Centrifugal Filter Unit (Millipore) in 2ml PBS.
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... GGG-azide labelled proteins were washed with Tris/NaCl buffer and concentrated to 25 µM on a 30 kDa Amicon Tube (Merck Millipore) and afterwards labelled with 100 µM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... the purified MECU protein was separated on 12% SDS-PAGE and further probed with mouse polyclonal anti-His (Sigma, USA) and rabbit polyclonal anti-H ...
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bioRxiv - Developmental Biology 2020Quote: Coding regions for all protein fragments used for EMSA were cloned in-frame with an N-terminal 6xHis-tag in the pET14b vector (Novagen) and expressed in BL21 cells ...
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bioRxiv - Genetics 2019Quote: ... The beads were washed 3x with wash buffer and proteins were eluted with FLAG peptide (200 mg/ml, Sigma-Aldrich) in TBS ...
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bioRxiv - Cell Biology 2021Quote: ... measuring its ability to prevent heat denaturation of two protein substrates: pig citrate synthase (CS) (0.2 μM; MW 51 kDa; Sigma Aldrich), and catalase purified from bovine liver (2 μM ...
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bioRxiv - Biochemistry 2020Quote: P-gp was incubated with a 10x molar excess of membrane scaffold protein (MSP) and a 500x molar excess of 40% L-alpha-phosphatidylcholine (PC) from soybean (Sigma) for 1 hour at room temperature with gentle agitation to facilitate formation of nanodiscs [73] ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration for the soluble fraction was adjusted (3µg/ml) following bicinchoninic acid assay measurement (BCA, Sigma-Aldrich, Poole, UK) and dilution in lysis buffer ...
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bioRxiv - Microbiology 2022Quote: Equal amounts of protein were resolved on an SDS-PAGE gel and subsequently transferred onto a polyvinylidene difluoride (PVDF; Millipore) membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...
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bioRxiv - Molecular Biology 2022Quote: ... the column was washed with 10 CV of binding buffer A and then the protein was eluted in binding buffer A containing 2.5 mM d-Desthiobiotin (Sigma-Aldrich). The eluted fractions were further purified using a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare) ...
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bioRxiv - Developmental Biology 2022Quote: ... For Western blot analysis the proteins were electrophoretically separated on a 10 % polyacrylamide gel and transferred onto a polyvinyldifluoride membrane (PVDF, Millipore). The PVDF membranes were blocked in 5 % nonfat dry milk diluted in Tris-buffered saline with 0.05 % Tween-20 for 1 h at room temperature and then incubated overnight at 4 °C with antibodies against FN (1:70000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (10 µg) were analyzed by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore) using Pierce™ 1-step transfer buffer (Thermo ...
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bioRxiv - Cell Biology 2022Quote: ... Conventional protein secretion was blocked (in indicated experiments) by incubating the cells with 10 μM Golgicide A (G0923, Sigma-Aldrich) 30 minutes prior to imaging ...
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bioRxiv - Cancer Biology 2022Quote: ... Hydrogels were made permissive for protein binding by incubating them in 500 µl of Sulfo-SANPAH solution [0.2 mg/ml Sulfo-SANPAH (Sigma, 803332) and 2 mg/ml N-(3-dimethylaminopropyl)-N′- ethylcarbodiimide hydrochloride (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNAs were purified by two rounds of immunoprecipitation with Dynabeads protein G conjugated to anti-BrdU (clone IIB5, Millipore). The purified cDNAs were then ligated using CircLigase II (Lucigen ...
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 protein eluent was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was then concentrated to ~200 μL with a 100 kDa molecular weight cutoff (MWCO) Amicon Ultracentrifugal filter (Millipore Sigma) at 1000 ×g ...
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bioRxiv - Bioengineering 2022Quote: ... a known quantity of EV protein was mixed with 1% ultra-low gelling temperature agarose (Sigma Aldrich, Oakville, ON, Canada), 1% gelatin (Sigma Aldrich ...