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Citations for Millipore Sigma :
9001 - 9050 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Protein fractions from a single peak were collected and concentrated in 50 kDa MWCO concentrator (Millipore Sigma), flash-frozen in liquid nitrogen and stored at −80 °C until further use ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 µM protein and 5 mg/ml of one of the following carbohydrates was used: maltose (Sigma), maltotriose (Carboexpert-BoGH13BSus/SusA ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were separated using SDS-PAGE and subsequently transferred to a polyvinylidene fluoride membrane (PVDF, Millipore Corporation). Then blocked PVDF membrane with blocking buffer (5% fat-free milk ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mL of the cleared lysate (∼2.5 mg total protein) was combined with anti-Flag (Sigma, 1804) coated Dynabeads Protein G (Invitrogen 10003D ...
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bioRxiv - Molecular Biology 2024Quote: ... Equivalent amounts of sample proteins in Laemmli buffer (With reducing agent, 2-Mercaptoethanol (Sigma-Aldrich, CAT# 8057400005) were electrophoresed on 4-20% Mini-PROTEAN® TGX Stain-Free gels (Bio-rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin concentrations were normalized among samples and pre-cleared with Protein G agarose beads (Millipore, 100 μL). The chromatin samples were split in two and diluted with IP buffer (10 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µg of protein was reduced with 5 mM Tris(2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma Aldrich) for 2 min and alkylated with 20 mM chloroacetamide (CAA ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted and precleared with 30 μL protein A/G agarose beads (Merck-Millipore, 16-157/16-201) for 1h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were further concentrated using Amicon Ultra centrifugal filter device (3 kDa MW cut-off, Millipore). Protein quantity was measured using Bradford method (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted protein solution was in turn transferred to an ultrafiltration column (Amicon Ultra-15, Merck Millipore) and concentrated by centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore). PVDF membranes were blocked with TBS-T (50 mM Tris ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purified protein was concentrated using an Amicon Ultra-4 Centrifugal 50 kDa Filter Units (Sigma Aldrich NV). Protein was quantified against a standard curve made with Bovine Serum Albumin (BSA) ...
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bioRxiv - Developmental Biology 2024Quote: ... that had been pre-coated with Protein A from Staphylococcus aureus (Sigma P6031; 100 ng/μl PA) and contained 800 μl culture media (Leibovitz’s L15 Medium ...
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bioRxiv - Molecular Biology 2024Quote: ... and in N2B27 serum-free media containing Mitogen-Activated Protein Kinase (MEK) inhibitor PD0325901 (1 μM) (Millipore), Glycogen Synthase Kinase 3 (GSK3 ...
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bioRxiv - Genomics 2020Quote: ... RRID:AB_2798177) overnight at 4°C or 1:10,000 dilution of monoclonal mouse anti-human β-actin (clone AC-74, Sigma-Aldrich, Cat. No. A2228, RRID:AB_476997) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: βII-spectrin-KO CRISPR cell lines were generated as follows: a predesigned vector containing a human βII-spectrin-targeting gRNA and eCas9-2A-tGFP was purchased from Millipore Sigma (MISSION gRNA ID: HSP00000399630). 24 hours after transfection of this plasmid ...
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bioRxiv - Bioengineering 2020Quote: ... MSCs embedded in the PEG-NB gels were stained for α-SMA with FITC-conjugated mouse monoclonal anti-human α-SMA (F3777, Sigma; dilution 1:300), cellular extra domain-A (ED-A ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll density gradient using Ficoll Plaque Premium (Sigma GE Healthcare, #17-544-02) or BD Vacutainer CPT Tubes (BD ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSC lines were dissociated to form embryoid bodies and cultured on mitomycin C-treated SNL murine fibroblast feeder cells in standard human embryonic stem cell culture (hESC) medium (DMEM/F12; Sigma Aldrich, St. Louis, MO, USA) containing 20% knockout serum replacement (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... codon optimized for expression in human cell lines and modified to include the human ER localization signal was cloned into the expression vector (Catalog Nr: OGS606-5U) under the EF1α promoter (Sigma Aldrich, St. Louis, MO, USA). Positive clones were verified by Sanger sequencing (Microsynth ...
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bioRxiv - Cancer Biology 2020Quote: ... On this plasmid a single primer PCR with primer 5’-GCA AGC CCT GAA AGC GCA AG-3’ and the cDNA human HIF-1α in a p3XFLAG-CMV™-10 expression vector (Sigma, St Louis, MO, USA) (Gort et al. ...
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bioRxiv - Immunology 2020Quote: ... plasma samples were incubated at a dilution of 1:100 and bound antibodies were detected with goat anti-human IgM/HRP (Sigma: cat. A6907, 1:6’000 dilution), goat anti-human IgG/HRP (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... and grown as undifferentiated 3D cultures in 500 µL IntestiCult™ Organoid Growth Medium (OGM) Human (STEMCELL™ Technologies, cat# 06010) supplemented with 10 µM Y-27632 (Sigma-Aldrich, cat# Y0503). Undifferentiated monolayers were produced by plating single cell suspensions obtained from 7 days old highly dense 3D cultures ...
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bioRxiv - Pathology 2023Quote: ... was performed in a representative subset of our participants using an adapted version of the MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel (Millipore Sigma, Burlington, MA, USA). The MAGPIX® Luminex analyzer (Luminex Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... COCs were placed in a 100-μl droplet of human tubal fluid (HTF) medium supplemented with 4 mg/ml BSA (Sigma-Aldrich, St. Louis, MO, USA)28 ...
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bioRxiv - Developmental Biology 2023Quote: ... following intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (∼ 90 hours pre-collect) and 5 IU human chorionic gonadotropin (hCG; Sigma; ∼40-48 hours pre-collect) and mating with BDF1 male mice (Jackson 100006) ...
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bioRxiv - Immunology 2024Quote: ... and IFN-γ were measured in the full Generation R cohort using the Human High Sensitivity T-Helper Cells Custom 5-plex assay from Millipore (Millipore, St. Charles, MO, USA) on the Luminex™ 100 system (Luminex ...
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bioRxiv - Microbiology 2024Quote: ... blood mononuclear cells were isolated by density gradient centrifugation from buffy coats of human healthy donors (Ficoll-Paque Premium, Sigma Aldrich, St. Louis, MI, USA). Monocytes were purified from peripheral blood mononuclear cells by positive selection with magnetic CD14 MicroBeads (Miltenyi Biotech ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Proteins were loaded on PVDF membranes ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cells in either radioimmunoprecipitation assay (RIPA) buffer 1 mM protease inhibitor cocktail (Sigma-Aldrich). Samples were heated at 100 °C for 10 min in a 4X loading buffer ...
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bioRxiv - Microbiology 2020Quote: ... The digested proteins were visualized in a 10% acrylamide gel stained with Coomassie Brilliant Blue R (Sigma Aldrich)
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to PVDF membrane (Immobilon-FL, pore size 0.45 μm, Millipore, catalogue number IPFL00010) for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... a fraction of Proteins was separated on 12% SDS-PAGE gels and transferred to Immobilon PVDF membranes (Millipore). The membranes were blocked in 5% milk or 5% BSA (depending on the primary antibody) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysates were cleared from insoluble chromatin and were subjected to immunoprecipitation with protein A agarose beads (Millipore) for 1.5 hrs at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The protein was concentrated using a 30 kDa MWCO Amicon ultra-15 centrifugal filter (Sigma, cat. no. UFC903024), and stored at −70°C ...
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bioRxiv - Immunology 2021Quote: ... The eluted protein was incubated with 0.5 mM ADP and 0.5 mM MCC950 (Millipore Sigma, Cat. no: 538120), 0.5 mM dATP or no additives for 30 min on ice ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ul protein A/G beads pre-saturated with 5 mg/ml BSA in PBS (Sigma, A-3311) were washed in RIPA buffer and subsequently added to the chromatin/antibody mix at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... and subsequently blocked with 0.5 % non-fat dried milk (AppliChem) in TBST using the Snap i.d.® 2.0 Protein Detection System (Millipore). The blot was then incubated for 10 min at room temperature with a 1:1000 dilution of rabbit polyclonal anti-ASIC1a antibody (OSR00097W ...
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bioRxiv - Neuroscience 2021Quote: ... protein extracts from SCN cultures were examined by SDS-PAGE immunoblotted with anti-UBE3A monoclonal antibody (E8655, Sigma), or anti-FLAG monoclonal antibody (F1804 ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of FLAG-AGO2 was performed with Dynabeads Protein G (Life) and anti-FLAG M2 mouse (Sigma, F3165) for 3h at 4ºC with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations for the whole cell lysates and the unbound fractions were determined using Bradford reagent (Sigma-Aldrich). For immunoprecipitation ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-GAPDH (1:2000, Protein Tech, #10494-1-AP) and anti-rabbit IgG-AP (1:20000, Sigma, #A3687)
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 5 packed bead volumes of 150 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS for 30 min at 4 °C with rotation.
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Cells were then incubated for 15 minutes in 2% BSA with globular actin-binding protein GC globulin (Sigma). Following incubation ...
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bioRxiv - Immunology 2022Quote: ... Single colonies were inoculated and protein expression was induced using isopropyl-β-D-thiogalacto-pyranoside (IPTG, Sigma Aldrich). Cell lysates were screened for protein expression using SDS-PAGE followed by Coomassie blue staining ...
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bioRxiv - Microbiology 2020Quote: ... ii) 300 μg C g−1 13C-labelled Algal crude protein extract-98 atom% 13C (ISOTEC, Sigma-Aldrich), iii ...
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bioRxiv - Immunology 2021Quote: ... Proteins were electrophoretically separated and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Merck Millipore, Burlington, MA, USA). Blocking treatment was performed using the PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein levels of wild-type or mutant Rad6 were determined by immunoblotting using anti-Flag M2 antibody (Sigma).