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Citations for Millipore Sigma :
9151 - 9200 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the surfaces of the coverslips were amino-modified with 3-(Trimethoxysilyl)propyl methacrylate (#M6514, Sigma-Aldrich, Saint-Louis, USA) to allow firm gel attachment ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were then rinsed 3 times with PBS followed by permeabilization with 0.1% triton X-100 (Sigma, X100) in PBS for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... cells were trypsinized than fixed with PBS/ formalin 3% then incubated with PBS/ Triton 0.1%/ TRITC-phalloïdin 1 µg/mL (Sigma Aldrich) during 5 min ...
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bioRxiv - Cell Biology 2021Quote: For analysing autophagy flux 72-hours old larvae were transferred to fresh medium containing 3 mg/ml chloroquine (Sigma) as described previously (Low et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Polysomes were eluted by resuspension of the washed gel in polysome wash buffer containing 3×FLAG peptide (Sigma-Aldrich). Total RNA was extracted from the final elution using Direct-zol RNA kit (Zymo research) ...
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bioRxiv - Plant Biology 2020Quote: ... Trypsin digestion was stopped either by heating at 95 °C for 3 min or by ultrafiltration using 0.5 ml Amicon Ultra centrifugal devices (20 kDa MWCO, Millipore). A five microliters aliquot was kept for MALDI-TOF MS analysis and the rest was used for the enrichment of biotinylated peptides by affinity chromatography.
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bioRxiv - Plant Biology 2021Quote: ... Col-0 plants grown on soil for 3-4 weeks were spray-treated with 0.5 mM SA (Sigma-Aldrich) and tissues harvested at indicated time-points for further analysis.
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bioRxiv - Plant Biology 2020Quote: ... The algal suspension was mixed into an equal volume of 3% (w/v) sodium alginate (Sigma, Product number 180947) solution then a syringe was used to generate 3 – 4 mm diameter algal-alginate beads by adding drops of the sodium alginate-algal mixture into a 2% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... The remaining 200 µL was transferred to a 3 kDa Amicon Ultra 0.5 mL filtering device (Millipore, Burlington, MA) for the quantification of AAs ...
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bioRxiv - Microbiology 2021Quote: ... amoebae were left unstained and incubated with either 3×106 or 1.5×107 fluorescent red carboxylate-modified polystyrene 2.0 μm latex beads (Sigma-Aldrich) or incubated in the absence of latex beads ...
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bioRxiv - Microbiology 2020Quote: ... Plates were then washed 3 times with PBST before the addition of 100ul of .5mg/ml o-Phenylenediamine (Sigma) diluted in phosphate-citrate buffer with sodium perborate (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... were immobilized on freshly made 3% agarose pad in a drop of M9 media containing 0.4 mM (0.05%) tetramisole (Sigma Aldrich) and 3.8 mM (0.5% ...
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bioRxiv - Microbiology 2021Quote: ... 50 µg of purified Cu-LpmP or CslZ protein were incubated for 3 h at room temperature with 0.2% peptidoglycan (PG) from Bacillus subtilis (Sigma), 5 mg α-chitin from shrimp shells (Sigma ...
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bioRxiv - Microbiology 2021Quote: Cells transfected with NL-43 or pNL4-3-△5’ were lysed using freshly made lysis buffer (0.5% Igepal CA-630, Sigma; 50 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: Seedlings were germinated vertically for 3 days on half strength MS plates containing 0.4% Gelzan™ CM (Millipore Sigma), and then transferred to plates containing either 0 (-P ...
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bioRxiv - Plant Biology 2021Quote: ... Fractions were desalted on C18 Cartridges (Empore™ SPE Cartridges C18, bed I.D. 7 mm, volume 3 ml, Sigma), and reconstituted in 0.1% (v/v ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then washed twice with PBS prior to 20 min permeabilization with 0.5% Triton X-100 and 1 h blocking with 3% BSA (Sigma) at room temperature ...
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bioRxiv - Microbiology 2020Quote: Biofilms were set up as described previously except they were incubated at 37°C in either 0.03% CO2 or 5% CO2 for 48h in RPMI-1640 containing varying concentrations of the Fe2+ chelator 3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p’-disulfonic acid monosodium salt hydrate (Ferrozine – Sigma-Aldrich, 160601) or the Fe3+ chelator Deferasirox (Cambridge Bioscience ...
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bioRxiv - Microbiology 2019Quote: ... The antigen bound Magnetic Beads were washed five times with the lysis buffer and eluted by 2.5 volume of 3×FLAG peptide (150 ng/µl, Sigma) for 3h at 4°C with gently shaking ...
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bioRxiv - Microbiology 2020Quote: ... before 3 washes with 1 mL PBS and saturation with a 0.5% BSA solution in PBS 1X (Sigma A7888) for 15 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: Sox14GFP/+ mouse brain samples (n=3) were embedded in a 4.5% oxidised-agarose solution containing agarose (type 1; Sigma), 10 mM NaIO4 (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... migrating/invading/transmigrating cells were incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 5 mg/ml, 1/10 volume; Sigma-Aldrich) added to the culture medium at 37°C for 4h ...
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bioRxiv - Cell Biology 2021Quote: ... series was made in a 96-well plate and cells were spotted onto YPD or YPD + 500μM auxin (indole-3-acetic acid dissolved in DMSO; Sigma) plates ...
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bioRxiv - Cell Biology 2020Quote: ... Media was changed every 3 days and cells were fixed two weeks after initial seeding with 0.5% glutaraldehyde (Sigma) in PBS for 20 min and left drying overnight ...
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bioRxiv - Biophysics 2020Quote: ... The protein was eluted from the column with Wash Buffer supplemented with 3 mg mL-1 1D4 peptide and concentrated to 4.3 mg mL-1 using a 100-kDa concentrator (Millipore) before further purifying by size exclusion chromatography using a Superose 6 increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... The cultures were then blocked for 1 hour at room temperature with 3% (w/v) BSA in PBS (Sigma), followed by incubation with anti-Gfap (1:100 ...
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bioRxiv - Biophysics 2020Quote: The GYMC52_3505 and pMHTDelta238 plasmids were transformed to Rosetta2(DE3)pLysS competent cells (Cat. No. 71401-3, EMD Millipore). Transformation reactions were spread on LB plates with ampicillin (Amp ...
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bioRxiv - Cell Biology 2020Quote: ... in DMEM solution for two 45 minute periods and transferred to 0.5 mg/mL Collagenase D in DMEM solution supplemented with 3% Liberase TL (Sigma) overnight ...
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bioRxiv - Cell Biology 2021Quote: Chromatin was isolated from supernatant by adding 3 volumes of TE with 1% SDS and RNAse A (Sigma R6513), and vortexing to mix ...
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bioRxiv - Cell Biology 2020Quote: ... Cell medium was replaced with 3 mL medium containing 20 μL of the purified lentivirus and 3μl polybrene transfection reagent (Merck Millipore). Medium was supplemented with 10 µg/mL puromycin for selection of successfully transduced cells two to three days after transduction.
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bioRxiv - Cell Biology 2021Quote: ... cells were washed 3 times in PBS and blocked in PBS supplemented with 0.5% fish skin gelatin (Sigma-Aldrich) for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3 times in staining buffer (PBS with 0.1% Bovine Serum Albumin (cat. num. A8806, Sigma), 0.05% NaN3 (cat ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK 293T cells were transfected with a 1:3 ratio of DNA:polyethylenimine (PEI, 25 kDa linear, Sigma-Aldrich). Transfection of siRNA was performed using X-tremeGENE siRNA Transfection Reagent (Roche ...
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bioRxiv - Biophysics 2022Quote: ... Both the CS and cantilevers were washed with acetone and functionalized using 2% (v/v) 3-aminopropyltriethoxysilane (Millipore Sigma) solution dissolved in acetone ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally washed 3 times in PBS-Tween 20 0.1% and mounted in Mowiol 4-88 (81381, Sigma).
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bioRxiv - Biophysics 2022Quote: PC-3 cells were seeded at 1,400 cells/100 μL in 96 well plates in RPMI media (Sigma-Aldrich) with 10% FBS ...
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Actin binding domain of Rng2 sparsely bound on F-actin strongly inhibits actin movement on myosin IIbioRxiv - Biophysics 2022Quote: ... This was followed by concentration with a centrifugal concentrator (Amicon Ultra-15 3 k device, Merck Millipore, Burlington, MA), and after supplementing with 10 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... the slides were washed 3 times for 5 mins while gently shaking at RT with PBS + 0.1% Tween (Sigma) and stained for 90 mins in blocking buffer with a combination of secondary antibodies ...
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bioRxiv - Systems Biology 2022Quote: ... and then a certain amount of culture (2~3 mg dry weight) was filtrated through a biomass filter (Durapore 0.45 μm, diameter 25-mm, Sigma). The filter with the cell pellet was immediately transferred to an aliquot of 4 mL of precooled extraction solution (−20°C ...
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bioRxiv - Plant Biology 2022Quote: ... Br-3’,5’-cAMP (B5386) and Br-adenosine (B6272) were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Br-2’,3’-cAMP was purchased from Biolog (Biolog Life Science Institute ...
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bioRxiv - Physiology 2022Quote: Cell viability was measured by the colorimetric assay showing reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich) as previously described (Denizot and Lang ...
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bioRxiv - Genetics 2022Quote: Fipronil (CAS# 120068-37-3) and Spinosad (CAS# 168316-95-8) were both obtained as analytical standards from Sigma. Insecticide bioassays were performed as described previously [20] ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich) as described [29].
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bioRxiv - Molecular Biology 2022Quote: ... cut into smaller pieces and homogenize in 3 ml of nuclei isolation buffer (nuclei EZ lysis buffer (Sigma, NUC101), 1x Halt™ protease inhibitor cocktail (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, San Louis, MI, USA) solution in PBS was added to the cells and incubated for an additional 4 h ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated to 2 mg/mL using centrifugal filters with either 3 kDa or 30 kDa cut-off (Amicon Ultra-0.5, Merck/Millipore) and the samples were centrifuged at 100,000 g ...
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bioRxiv - Neuroscience 2022Quote: ... GLASTCreERT2;Sun1-GFP mice at ∼3 weeks of age were intraperitoneally injected with 4-OHT (Sigma-Aldrich, #H6278-50mg) in corn oil (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... and 0.02% (w/v) yeast extract for 15N labelling or 15NH4C1 and 13C6 D-glucose (3 g/L, Sigma) with 0.02% (w/v ...