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Citations for Millipore Sigma :
9101 - 9150 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Primers were designed using Primer3 software62 with sequences obtained from GenBank and synthesized by Sigma Aldrich (Table 3, 4). PCR reactions were performed on the LightCycler®480 Real Time PCR System (Roche ...
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bioRxiv - Bioengineering 2019Quote: ... The glass micropipettes were surface-modified through silanization with N-(2-aminoethyl)- 3-aminopropyltrimethoxysilane (EDA) (Sigma Aldrich catalog # 440302) in order to reduce stiction forces between polysilicon and the wall of the miniaturized glass micropipette during their integration ...
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bioRxiv - Plant Biology 2019Quote: Treatments with the NO-scavenger 2-(4-Carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazol-1-yloxy-3-oxide potassium salt (c-PTIO salt, Sigma Aldrich, Darmstadt, Germany) were performed 1 hour prior to ethylene treatments to allow for treatment combinations ...
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bioRxiv - Neuroscience 2019Quote: ... 4AP dissolved in ddw to 200 mM (30 μM/L – 3 mM/L; Millipore Sigma, Burlington, MA, cat. #275875) was used to block fast activating fast inactivating potassium currents ...
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bioRxiv - Microbiology 2019Quote: ... The spheroplasts were then washed 3 times in SPM buffer permeabilized by incubation in 1 mL of 0.1% triton X-100 (Sigma) in PBS for 2 minutes on ice and washed twice in PBS ...
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bioRxiv - Immunology 2019Quote: ... The experiment was initiated by adding 3 µCi [9,10−3H]-palmitic acid complexed to 5% BSA (lipids free; Sigma) and ...
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bioRxiv - Evolutionary Biology 2019Quote: ... were toothpicked onto fresh BG11 agar plates containing 0.015 g/L of L-methionine and different concentrations of 3-deazaneplanocin A hydrochloride (Sigma), sinefungin (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... fixed with an ice-cold glutaraldehyde-based solution in CB buffer (0.05 % glutaraldehyde, 3 % formaldehyde and 0.1 % TritonX; Sigma Aldrich) for 15 min on ice ...
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bioRxiv - Cell Biology 2019Quote: ... Larvae were anesthetized prior to experimental procedures in E3 water containing 0.2 mg/ml Tricaine (ethyl 3-aminobenzoate, Sigma). To prevent pigment formation in larvae during imaging experiments ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 3 hr in 5% milk in TBS (50 mM Trizma base and 150 mM NaCl, PH 8.3, both Sigma) plus 0.05% Tween-20 (Sigma ...
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bioRxiv - Immunology 2019Quote: Cell viability was monitored by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT; Sigma, USA) assay as previously described [52] ...
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bioRxiv - Physiology 2020Quote: The reduction of yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich, Germany) was used to measure cellular metabolic activity as a proxy for cell viability [19,22] ...
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bioRxiv - Developmental Biology 2019Quote: ... phenylarsine oxide (Lot#056K1654) and Rutin hydrate (quercetin-3-rutinoside: ≥94%[HPLC] Lot#BCBH6323V) were purchased from Sigma-Aldrich. T3 (3,3’,5’ triiodo-L-thyonine ...
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bioRxiv - Plant Biology 2019Quote: ... 150 mM NaCl and 10 mM HEPES pH 7.4 and eluted with 100 μg ml−1 3×FLAG peptide (Sigma).
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bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were cleaved off the column with thrombin diluted 1:250 in thrombin cleavage buffer (Novagen #69671-3). The thrombin solution was loaded manually into the column with a syringe ...
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bioRxiv - Immunology 2019Quote: ... Embryos were grown at 28.5°C and kept under anesthesia with egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma) during bacterial injections ...
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bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 40ul of 20mg/mL X-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside – Sigma Aldrich B44252) and 40ul of 100mM IPTG (isopropyl β-D-1-thiogalactopyranoside – AppliChem A4773 ...
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bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were resuspended in 5 µL of cold lysis buffer (10 mM Tris-HCl [pH 7.4], 10 nM NaCl, 3 nM MgCl2, 0,1% v/v Igepal CA-360 [Sigma-Aldrich] and incubated on ice for 5 min ...
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bioRxiv - Bioengineering 2021Quote: Sections were incubated in a permeabilization and blocking solution of 0.3% Triton X (A4975, PanREAC AppliChem) and 3% BSA (A7906, Sigma) in PBS for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane, 2 membranes per animal, 0.4 μm Millicell, Merck Millipore) in 50% BME (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... 10 ml of alkaline phosphatase (100mM Tris-HCl pH 9.5, 100 mM NaCl, 3 mM MgCl2) containing 80 μl NBT (Sigma) (35mg/ml in70% DMSO and 30 μl de BCIP (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at 4°C in a humid chamber with primary antibodies (Table 3) in 0.1 M PBS containing 10% normal horse/goat serum and PBS-0.3% Triton X-100 (Sigma-Aldrich). After primary antibody incubation ...
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bioRxiv - Cell Biology 2021Quote: ... 1uM each of gRNA (IDT) were mixed with spCas9 at 3:2 ratio in embryo grade water (W1503, Sigma) and injected into wild type C57BL6 zygotes at one-cell stage ...
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bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Bioengineering 2021Quote: ... pH 7.4 by centrifugation through 3 kDa molecular weight cut off centrifugal filters (Amicon Ultra®, Millipore, Dublin, Ireland). Absorbance at 555 and 280 nm was measured for labelled samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 to enable quantification of relative protein concentration and label substitution efficiency ...
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bioRxiv - Bioengineering 2020Quote: ... cells (15·106 mFBs·cm−3 and 30·106 THP-1 cells·cm−3) were added to a mixture of bovine fibrinogen (10 mg·ml−1; Sigma, F8630) and bovine thrombin (10 IU·ml−1 ...
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bioRxiv - Bioengineering 2020Quote: ... we added 3% v/v photoinitiator Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (Sigma-Aldrich, “LAP”, 39.2 mg/mL in DI water). Produced Ln/polymer droplets were collected through Tygon tubing into a 24-well plate (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... after a 60 min incubation at 25 °C the reactions were stopped by addition of 3 μl 3.3 × LDS sample buffer (Sigma) containing NEM (40 mM final concentration ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Biochemistry 2020Quote: ... incubated with Oil Red O working solution (3:2 Oil Red O: ultrapure water) for 20 minutes (Sigma O1391), and washed with 5 changes of ultrapure water.
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bioRxiv - Neuroscience 2020Quote: ... Cells were released via 0.12% trypsin without EDTA and washed 3 times with PBS (Sigma-Aldrich, St. Louis, MO). 0.4% trypan blue (Thermofisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... A 96-well PCR plate was prepared with one 3 mm diameter borosilicate solid-glass bead (Millipore Sigma #Z143928) and 100 µl PBS in each well ...
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bioRxiv - Neuroscience 2021Quote: ... and immediately fused to 18 mm glass coverslips (#1.5) that had been treated with 3-aminopropyl-triethoxysilane (Sigma 440140). Samples were washed with PBS 3x 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... The rest was incubated with 30μl of Protein G Dynabeads and 3 μl of anti-FLAG M2 antibody (Sigma) overnight to immunoprecipitate (IP ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability was determined using the 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, Milan, Italy) according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2021Quote: ... gRNA sequence: 5’-ACTTGGCCAAGGAGTACGG-3’) in line with GFP from the (p04) U6-gRNA:CMV-eCas9-2a-tGFP plasmid (Sigma). One day later ...
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bioRxiv - Cell Biology 2021Quote: ... and cells were isolated by digesting the pulp tissue with collagenase type I (3 mg/ml, Sigma-Aldrich, Germany) for 60 minutes at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... the culture medium was removed and cells were rinsed 3 times with Dulbecco’s Phosphate Buffered Saline (DPBS, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... CM (and the same volume of SM as control) was then concentrated by 3 kDa Amicon filters (Merck-Millipore) to a final volume of 100 μl/mouse and stored at −20°C until usage ...
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bioRxiv - Cancer Biology 2020Quote: ... Viability was evaluated with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) colorimetric assay (Sigma, #M5655).
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bioRxiv - Cell Biology 2021Quote: ... The target cell lines at 50-60% confluency were then infected using 3 mL virus with polybrene reagent (Sigma). After 24 hours ...
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X-100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Mice were fed ad libitum or fasted at ZT0 and given CL316243 (3 mg/kg body weight; Sigma Aldrich) or vehicle (0.5% carboxymethylcellulose in sterilized water ...
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bioRxiv - Plant Biology 2020Quote: ... epitopes were blocked with 0.1 M PBS / 1% Cold Water Fish Skin Gelatin / 0.2% BSA with 3% sucrose (all chemicals from Sigma Aldrich), incubated with the primary antibody anti-GFP (1:250 ...
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bioRxiv - Biochemistry 2021Quote: ... The C-terminal domain (CTD) of DRH-3 (residues 940-1108) was cloned into the expression vector pET15b (Novagen), and the resulting clone carries an N-terminal His6 tag and a thrombin cleavage site ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM sodium phosphate pH 8.0, 3% glycerol, 1% Triton X-100, 15 mM imidazole, and 1x protease inhibitor [Roche, Sigma]) and sonicated ...
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bioRxiv - Biochemistry 2020Quote: ... each N-Cdh sample was desalted against PBS using Amicon centrifugal filters with a 3 kDa molecular weight cutoff according to the manufacturer’s protocol (Merck-Millipore). Unprocessed xCGE-LIG N-glycocomics raw data are available upon request.
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bioRxiv - Bioengineering 2021Quote: ... 0.1% solution of Triton X-100 in PBS (v/v) and 3% Bovine Serum Albumin solution (Sigma Aldrich, A3803) in PBS (w/v ...