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Citations for Millipore Sigma :
9401 - 9450 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The linear DNA backbone was then extracted from the gel via electroelution in D-tubes (EMD Millipore 71508-3), followed by phenol chloroform extraction and butanol concentration.
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and 3-(4,5-dimethyl-2-thiazolyl)2,5-diphenyl-2-H-tetrazolium bromide (MTT, Sigma-Aldrich) solution (final concentration ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by staining of ossified bones with a 50 mg/l of alizarin red S (Sigma, #130-22-3) solution in 2% KOH for 3 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... reconstituted in 3 µL of 66% acetonitrile and analyzed by a ZIC-HILIC column (150 × 2.1 mm, EMD Millipore) coupled with a Q Exactive HF Orbitrap MS (Thermo Fisher ...
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bioRxiv - Biophysics 2024Quote: ... Mice were decapitated and the nose was dissected en bloc and embedded in 3% low-grade agar (A7002, Sigma) prepared with Ringer’s saline solution containing (in mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs were seeded into the collagen gel channel with media containing 3% dextran (Leuconostoc spp., Mr 450,000–650,000, Sigma-Aldrich) at a density of 1 × 107 cells/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Flat mounts were incubated in 300 µL of blocking buffer (3% (W/V) bovine serum albumin (BSA) (Catalog #BP9703-100) and 0.1% (V/V) Triton X-100 (Sigma) in HBSS (Fisher Scientific Catalog # MT21023CV ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with blocking buffer (PBS containing 0.1% Triton X-100 and 3% fatty-acid-and globulin-free BSA [A7030, Lot No. SLCH7959, SIGMA]) for 1 h at room temperature (approximately 26-28°C ...
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bioRxiv - Microbiology 2024Quote: ... Coloring reactions were performed with nitro blue tetrazolium/5-Bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) solution (Sigma), and then sections were washed in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
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bioRxiv - Plant Biology 2024Quote: ... After immunolabeling the coverslips were incubated in Acryloyl-X-SE 0.1 mg/mL in PBS (ThermoFischer) overnight at RT and then in the monomer solution (11.7% NaCl, 3 % acrylamide (Sigma), 0.15% N,N′-methylenebisacrylamide (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
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bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
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bioRxiv - Neuroscience 2024Quote: ... cells grown in 96-well plates were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, Hungary) in a final concentration of 200 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... pregnant females were ovariectomized on E2.5 and afterwards injected with 3 mg medroxyprogesterone 17-acetate subcutaneously (Sigma, M-1629) on E3.5 and E5.5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich (St Louis, MO). Phalloidin-iFluor 594 was purchased from Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... were washed 3 x with PBS followed by incubation with 20 μg mouse anti-FLAG M2 Ab (Sigma Aldrich) for 2 h at 4 °C with rotation prior to 3 x PBS washes and resuspension in PBS ...
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bioRxiv - Genetics 2024Quote: ... gcry1:BFP-3’mitfaHom plasmid was then added to the injection mix along with phenol red (Sigma-Aldrich P0290). The final concentrations injected into the embryos were ...
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bioRxiv - Cell Biology 2024Quote: ... permeabilized using 0.2% Triton-X100 in 1x phosphate buffered saline (PBS) for 15 min and blocked using 3% BSA (Sigma) in 1x PBS for 30 min ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed to add the 3XFlag tag to the N terminus of ZBP1 cDNA at the 5’ Not1 site and 3’ Sal1 site to facilitate cloning into expressing vector p3XFlag-CMV-7.1 (Sigma). The resulting plasmid containing the 3XFlag-ZBP1 was further subcloned in the Bst1 and BamHI sites of the vector pLVX-EF1α-AcGFP1-C1 (Takara ...
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bioRxiv - Microbiology 2024Quote: ... after which the remaining supernatant (2 x 45ml) was collected and concentrated over a 3 kDa Amicon filter (Millipore). The residue was suspended in a volume of 900 µl ...
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bioRxiv - Microbiology 2024Quote: ... The female individuals were anesthetized with 0.02% (w/v) ethyl 3-aminobenzoate methanesulfonate (Sigma-Aldrich, St. Louis, MO, USA), euthanized by decapitation ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
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bioRxiv - Microbiology 2024Quote: ... Oat seedlings for purification and amplification were treated with 3 mM maleic hydrazide (Sigma-Aldrich, St. Louis, MO, USA) at emergence ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... or 250 nM Compound 3 were fixed and stained for mouse anti-γ-tubulin 1:500 (Sigma Aldrich, T5326), rabbit anti-KIF18A 1:100 (Bethyl ...
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bioRxiv - Neuroscience 2023Quote: ... A 1-in-3 series was collected directly onto gelatin-coated slides and Nissl-stained (cresyl violet, Sigma-Aldrich) for verification of electrode location.
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bioRxiv - Microbiology 2023Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Microbiology 2023Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report65 ...
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bioRxiv - Cell Biology 2023Quote: ... Treatment of mice were performed with a single intraperitoneal injection of either vehicle (corn-oil) or 3 mg/kg 1,4-bis-[2-(3,5,-dichloropyridyloxy)] benzene (TCPOBOP, T1443, Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... Now the samples were permeabilized and blocked by incubating with 3 % donkey serum (Catalog no. D9663-10ML, Sigma Aldrich) and 2 % TritonX (Catalog no ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Target cells were transduced with 0.5 mL of viral supernatant in 3 mL of total medium supplemented with 8 µg/mL polybrene (Merck Sigma). 2 to 4 days post transduction ...
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bioRxiv - Genomics 2023Quote: ... 24 hours prior to induction the cells were cultured in 1 mM indole-3-acetic acid (IAA; Sigma, I5148), to ensure protein degradation ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated on ice for 3 min and 2 mL wash buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1 % Tween-20, 1 % BSA, 1 mM DTT, 1 U/µL Sigma Protector RNase inhibitor in nuclease free water ...
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bioRxiv - Neuroscience 2022Quote: ... The dopamine- synthesis inhibitor 3-Iodo-L-tyrosine (3IY; CAS: 70-78-0, Sigma Aldrich; concentration: 5 mg/ml) was added to samples of 2 ml yeast solution ...
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bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report63 ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Genomics 2022Quote: ... An antibody specific for GroEL (rabbit polyclonal, 1:10,000 in 3% BSA/TBS-T; Sigma-Aldrich, #G6532-5ML, RRID:AB_259939) with an anti-rabbit IgG (goat polyclonal ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were permeabilized with 0.01% Triton X-100 in PBS for 15 minutes and blocked with 3% bovine serum albumin (A7906, Sigma) in 0.01% Triton X-100 in PBS (blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... embryos were mechanically dechorionated and anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine, Sigma-Aldrich). Zebrafish embryos were individually infected by microinjection with 1 nl of P ...
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bioRxiv - Bioengineering 2022Quote: ... 300 μL of supernatant was filtered under 3 kDa through Amicon Ultra-0.5 mL Centrifugal Filters (Millipore, Burlington, MA) or 96-well plates (Pall Corporation ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (60) ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h in 20 mL TBS-T with 3% milk powder and 1:10,000 diluted goat anti-rabbit IgG-HRP (Sigma). Finally ...
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bioRxiv - Immunology 2023Quote: ... RIPA 400 mM NaCl, (3) Sarkosyl Buffer (2 mM EDTA, 50 mM Tris-HCl, 0.2% sarkosyl (Lauroylsarcosine sodium salt (Sigma)) ...
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bioRxiv - Microbiology 2022Quote: ... cultures were pelleted by centrifugation at 1,600 rpm for 3 min and resuspended in a 10× volume of 5% sorbitol (Sigma), followed by incubation at 37°C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... and AP2XII-1-AID-HA /AP2XI-2-mAID-MYC was achieved with 3-indoleacetic acid (IAA, Sigma-Aldrich # 45533). A stock of 500 mM IAA dissolved in 100% EtOH at a ratio of 1:1,000 was used to degrade mAID-tagged proteins to a final concentration of 500 μM ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Neuroscience 2023Quote: ... was concentrated to under 100 μL in 0.5 mL 3 kDa molecular weight cut-off centrifugal concentrators (Millipore, UFC500396) (Surface sample in Fig 4C) ...