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Citations for Millipore Sigma :
9001 - 9050 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... samples were washed in PBS and permeabilised in PBS supplemented with 3% FBS and 0.1% Saponin (Sigma-Aldrich) for 5 minutes at RT ...
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bioRxiv - Biophysics 2023Quote: ... antisense strand: 5’-GUA UCU CUU CAU AGC CUU A(dT)(dT)-3’) were synthesized by Sigma Aldrich. Early Endosome Antigen 1 (EEA1 ...
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bioRxiv - Cell Biology 2023Quote: ... Imidazole was removed by 3 cycles of concentration and dilution using Vivaspin (Millipore Sigma, Cat# GE28-9322-25). TEV enzyme was added at about 1/10 of the target protein mass and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... membranes spotted with different lipid species were blocked with 3% fatty acid free BSA (w/v; Sigma, 7030) in 0.1% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... the input media were centrifuged at 3,200 g for 30 min with 3 kDa MWCO filter units (Millipore); after centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitors (prepared in-house according to Phosphatase inhibitor cocktail 1, 2 and 3 from Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed with 0.5% glutaraldehyde and incubated for 3 min at RT with 5 mg/ml sodium borohydride (Sigma). After washes ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
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bioRxiv - Plant Biology 2024Quote: Leaves of 3-4-week-old Nicotiana benthamiana plants were agroinfiltrated and sprayed with 10 μM estradiol (Sigma) 24 h after infiltration to induce protein expression ...
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bioRxiv - Bioengineering 2023Quote: ... 1000 μM) prepared from a 0.5 M stock solution of 3-MB (Sigma-Aldrich, St Louis, MO, USA) solubilized in a 0.5 M NaOH solution and filtered through a 0.2 μm filter for sterilization (Minisart 0.2 μm syringe filter ...
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bioRxiv - Microbiology 2024Quote: ... a collagen raft mixture was prepared on ice via combining 3 mg/ml collagen (Millipore-Sigma, #08-115) with 10X E-Media (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... a collagen raft mixture was prepared on ice via combining 3 mg/ml collagen (Millipore-Sigma, #08-115) with 10X E-Media (DMEM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: PESTANAL® analytical grade tefluthrin (2,3,5,6-tetrafluoro-4-methylbenzyl 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylic acid) was purchased from Sigma Aldrich. Tefluthrin stock solutions were prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2024Quote: 1.5 x 106 YTS NK cells were cultured in 3 mL of media containing 1.8 mL of previously obtained viral media and 8μg/mL polybrene (Sigma). The culture plates were spun for 2 hours at 400 x g and then placed in an incubator overnight at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Stained zebrafish larvae were anesthetized with 0.016% (m/v) Ethyl 3-aminobenzoate methanesulfonate salt (Tricaine; Sigma-Aldrich, A5040) and embedded in 1.8% low melting point agarose (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... The number of viable cells was quantified using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; M2128, Sigma) assay at 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... corrected for saturation and normalized to an external reference (sodium 3-(trimethylsilyl) propionate-2,2,3,3-d4 (TSP; Sigma-Aldrich)) and to cell number (64).
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bioRxiv - Microbiology 2024Quote: ... After 24 h media/inhibitor was aspirated and replaced with 20 μl of 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) and incubated at 37°C in 5% CO2 for 3 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Bands were stained in 5-bromo-4-chloro-3-indolyl-phosphate and nitro blue tetrazolium solution (Sigma-Aldrich). The membrane image was acquired using a digital steel camera EOS M6 mark II with a lens EF16-35 mm F4L IS USM (Canon Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... slides were cleared in Xylene (2 × 3 min) and coverslipped with DPX mounting medium (Cat# 44581, Sigma-Aldrich). Sections were photographed with an epifluorescence microscope (Model BX51 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total of 150 µL of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone; Sigma-Aldrich) dissolved in 100% ethanol (Wako ...
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bioRxiv - Bioengineering 2024Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and collagenase type 1 was purchased from Sigma-Aldrich. RNA extraction kit (Iran) ...
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bioRxiv - Cell Biology 2024Quote: Treatments for the LC3 assay included 3 hours with mTOR inhibitor Torin1 (Merck-Millipore, final concentration 250 nM), Bafilomycin A1 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 min at RT and then permeabilized for 3 min with 0.2% Triton-X 100 (Millipore Sigma) Over-extracted cells were treated with 0.4% Triton-X 100 ...
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bioRxiv - Cancer Biology 2024Quote: ... the PDMS stamp is placed on a silanized coverslip pretreated with 2% (3-Trimethoxysil) propyl-methacrylate (Sigma #M6514)) ...
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bioRxiv - Genetics 2024Quote: Adult zebrafish (3 months post fertilization) were euthanized using a 25x tricaine solution (4000 mg tricaine powder (Sigma), 979 ml distilled water ...
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bioRxiv - Microbiology 2024Quote: ... the animal’s drinking water was replaced from days 3-8 with water containing 5% L-arabinose (Sigma Aldrich). InducTn-seq samples were collected 8 days post inoculation by plating fresh feces on solid LB containing 50 µg/ml kanamycin ...
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bioRxiv - Microbiology 2024Quote: ... They were washed three times with PBS and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich, A3294) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was applied to 3 kDa MWCO filters (Amicon Ultra-0.5 Centrifugal Filter; Merck Millipore, Darmstadt, Germany), and centrifuged at 14,000 × g for 45 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... They were maintained in a Petri dish containing embryo medium complemented with 3% Methylcellulose (Sigma-Aldrich, USA, #274429) to allow dorsal and lateral pictures of the whole body on glass microscope slides ...
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bioRxiv - Cancer Biology 2024Quote: ... 3-(2-Methyl-5-nitro-imidazol-1-yl)-N-(2,2,2-trichloro-1-phenylamino-ethyl)-propionamide) (Sigma-Aldrich SML1503). Drugs were diluted in culture media at treatment.
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bioRxiv - Cancer Biology 2019Quote: ... and HEK293T embryonic kidney cells [6] were grown in DMEM supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin (Sigma Aldrich, St. Louis, MO). All cells were cultured at 37°C in 5% CO2 and 5% O2 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the mothers of the experimental animals returned to their home cages and were treated daily with a stainless steel feeding needle per oral gavage with either fluoxetine (10 mg/kg, n= 6 dams) or vehicle (Methylcellulose 1%, (Sigma, St. Louis, MO, USA), n=4 dams ...
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bioRxiv - Bioengineering 2020Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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bioRxiv - Neuroscience 2021Quote: ... CAS # 7646-85-7 and ZnC4H6O4; CAS #557-34-6) and arsenic (NaAsO2; CAS #7784-46-5) (all from Sigma-Aldrich, St Louis, MO). The metallic compounds were either dissolved in 30% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 cm or 10 cm cell culture dishes or 96 well flat-bottom plates (Thermo Fisher Scientific, Germany; Sigma-Aldrich, Germany; TPP AG, Switzerland). For analyses using the Operetta high content imaging system cells were plated with 10,000-50,000 cells/ml in 96-well µClear plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged in a 6 well plates and once confluent transduced with LV-EF1α-rtTA3/PGK-Neomycin plus LV-tetO-MyoD/PGK-Puromycin at 1:1 ratio and supplemented with 6 μg/mL of polybrene (Sigma-Aldrich, TR-1003-G). Transduced MEFs were expanded and selected by sequential antibiotics treatment with MEF medium containing either 1 mg/mL of G-418 solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 132 mM NaCl, 6 mM KCL, 1 mM CaCL2, 1 mM MgSO4, 1.2 mM K2HPO4, 5 mM glucose, Sigma-Aldrich, and 0.4% (w/v) human serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... for wild type or mutant BAG3-GFP and subjected to 6 h dynein inhibition with Dynarrestin (25μM, SML2332-5MG, Sigma-Aldrich, Saint Louis, MI, USA) or erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed with Tris-buffered saline/Tween 20 and then incubated with an anti-acetyl-α-tubulin antibody (Lysine-40) (Sigma Clone 6-11B1), α-tubulin (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were stained with 1 μg/ml 4′,6-Diamidino-2-phenylindole dihydrochloride in distilled water (D8417; Sigma-Aldrich, St. Louis, MO, USA), cover-slipped ...
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bioRxiv - Neuroscience 2022Quote: ... was performed on a 1 in 6 series of sections from sham- or 6-OHDA-lesioned animal as previously described[57] using an anti-TH mouse monoclonal antibody (LNC1 clone, EMD Millipore, Burlingame, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Genomics 2022Quote: ... slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (4′,6-diamidino-2-phenylindole, Sigma-Aldrich, St. Louis, MO, USA) in antifade based on DABCO (1,4-diazabicyclo(2.2.2)-octane ...
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bioRxiv - Immunology 2021Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO). They were then stimulated with peptide pools for 12 h ...
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bioRxiv - Bioengineering 2021Quote: ... a suspension of collagen and GAGs were produced by combining microfibrillar, type I collagen (Collagen Matrix, Oakland, NJ) and chondroitin-6-sulfate (Sigma-Aldrich, St. Louis, MO) with calcium salts (calcium nitrate hydrate ...
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bioRxiv - Genomics 2022Quote: ... 70% - 80% confluent cells from one well of a 6-well plate were dissociated to obtain single cell suspension and then resuspended in freezing medium (10% DMSO (Sigma Aldrich, Cat. No. D2650), 20% FBS (Millipore ...
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bioRxiv - Microbiology 2019Quote: B. burgdorferi strain B31 was cultured in BSK-H medium (HiMedia Laboratories Pvt. Ltd.) with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA). All culture medium was filter-sterilized by 0.2 µm filter ...