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Citations for Millipore Sigma :
9051 - 9100 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cells were disrupted by stirring for 1 hour in lysis buffer (100 mM Tris, 150 mM NaCl, 10% glycerol, 6 mg/mL lysozyme (Sigma, St. Louis, MO, USA), 2 mg/mL deoxycholic acid ...
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bioRxiv - Cell Biology 2021Quote: ... We used following combinations of primary (dilution 1:200) and secondary (dilution 1:250) antibodies: mouse monoclonal anti-acetylated tubulin (Sigma, [6-11B-1], T7451) with Abberior Star 635P goat anti-mouse IgG (H + L ...
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bioRxiv - Microbiology 2021Quote: ... medium (HiMedia Laboratories Pvt Ltd.) was filter-sterilized with 0.2 μm filters and supplemented with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA) for the culturing of B ...
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bioRxiv - Immunology 2020Quote: ... Pellet was resuspended in DMEM/F12 media and plated at 250,000 cells/mL on 6-well plates that had been pre-coated with 100 mg/ml PDL (Sigma-Aldrich, cat # 27964-99-4) at 37°C overnight ...
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bioRxiv - Genomics 2022Quote: ... Cells were plated at 1 x 105 per well in a 6-well plate (FALCON, Cat.no. 353046) clonal density in 2i culture medium (15% FBS (Millipore, Cat. No. ES-009-B), 1X Glutamine (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were stained by a PBS-based staining solution of 2 μM Calcein-AM from Thermo Fischer Scientific (Waltham, USA) for viable cells and 6 μM Ethidium homodimer-1 from Sigma Aldrich (St.Louis, MO, USA) for dead cells for 30 min at room temperature in the dark ...
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bioRxiv - Developmental Biology 2022Quote: ... the sperm pellet was washed in IVF–TALP medium and a final concentration of 1 × 106 spermatozoa/mL was adjusted using IVF–TALP medium enriched with BSA (Sigma A8806; 6 mg/mL) and heparin (25 mg/mL).
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bioRxiv - Microbiology 2022Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 supplemented with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO) at 37°C followed by stimulation with peptide pools for 12 h ...
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bioRxiv - Microbiology 2022Quote: ... 6-Carboxyfluorescein filled liposomes were separated from the unincorporated 6-Carboxyfluorescein by size exclusion chromatography using Sephadex G 25–300 (Sigma Aldrich, Saint Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... CreERT2 expression was activated in young adult mice (6-8 week-old) by administering tamoxifen (Sigma, T5648-1G, 20 mg/ml in corn oil) by subcutaneous injection (100mg/kg ...
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bioRxiv - Physiology 2024Quote: ... Nuclear stains used in this study were 4’,6-diamidino-2-phenylindole (DAPI; final concentration 1 μg/ml Sigma-Aldrich, St. Louis, MO, USA). F-Actin was stained with Alexa Fluor 568 phalloidin (1:200 ...
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bioRxiv - Cell Biology 2023Quote: Non-cornmeal molasses food was freshly made with or without 100 µM or 5 mM Phloroglucinol (PHG) (Sigma-Aldrich, CAS number 108-73-6) and solidified in a vial overnight ...
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bioRxiv - Immunology 2024Quote: ... and culture supernatants were harvested after 24 h for analysis of cytokines (CXCL1 and IL-6) and MMP2 activity (Luminex bead array, Millipore Sigma, St. Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... One group of mice (referred to as DMI + 6-OHDA) was pre-treated with one injection of desipramine hydrochloride (DMI, Sigma-Aldrich, 25mg/kg i.p.) 30 minutes before the 6-OHDA infusion in order to protect the noradrenergic system [50].
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bioRxiv - Neuroscience 2023Quote: Rescue of cholesterol accumulation were performed by adding the drugs to cultures previously incubated overnight with Bodipy-cholesterol and further incubated for 6 h at the following concentrations: Evodiamine (Sigma, stock 1000x in DMSO) 10 µM ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then centrifuged at 4,000 rpm for 22 min at 4°C and the supernatants were transferred to another set of 6 eppendorf tubes containing a mixture of pre-washed beads (Millipore Sigma, GE17-0886-01) and 12.5 uL (12.5 ug ...
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bioRxiv - Microbiology 2023Quote: ... The extracts were diluted from 1:10-3 to 1:10-6 in 10 mM MgCl2 for plating so that spreading of 50 μl extract with a delta cell spreader (Sigma-Aldrich, St. Louis, USA) resulted in a density of 100-300 CFU on square plates (12 x 12 cm ...
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bioRxiv - Physiology 2023Quote: Hepatocytes were isolated from C57BL/6 and SIRT4 KO mice (8–12 weeks old) by perfusing the liver tissue with collagenase type IV (Sigma-Aldrich, catalog no. C5138) containing media as described in Shaw et al ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were placed on glass slides (Matsunami, Osaka, Japan) and stained with 4’,6-diamidino-2-phenylindole (DAPI; D9542, Sigma-Aldrich, St. Louis, MO) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells (5 × 104 cells/cm2) were grown in triplicate in 24-well plates pre-coated with 6 μg fibronectin (Sigma-Aldrich catalog number FC010) and transfected the next day with 500 ng pRL0381 or pRL0385 with TransIT-LT1 ...
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bioRxiv - Microbiology 2023Quote: ... the media was replaced and supplemented with 200 ng/mL aTc and either DMSO or 6 μg/mL brefeldin A (Sigma-Aldrich catalog number B6542). After an additional 16 h ...
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bioRxiv - Immunology 2023Quote: ... bone marrow cells were obtained from femurs and tibiae of WT C57BL/6 mice by flushing with complete RPMI 1640 media containing 10% heat inactivated fetal bovine serum (FBS) (Sigma-Aldrich, non-USA origin). Red blood cells were lysed using ACK lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... neurospheres were collected or attached to standard culture plates in media containing 5% FBS for 6 h and stained with crystal violet solution (Sigma-Aldrich, 548-62-9).
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibody was washed with PBS++ including 4’,6-diamidino-2-phenylindole for initialization (DAPI; 1:10 000, Sigma Aldrich, St. Louis, MS, USA). Where needed ...
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bioRxiv - Microbiology 2020Quote: ... treated 3T3 mouse fibroblast feeder cells and grown until 70% confluency in the 3:1 mixture of Ham’s F12/DMEM media (HyClone) supplemented with 5% FBS (Sigma), 24 μg/mL adenine (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions from size exclusion column were pooled and concentrated using 3 kD cutoff ultracentrifugal device (Millipore, USA). The EspC purity was checked on 12% SDS-PAGE and by mass spectrometry ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 cells at ∼70% viability were spun down and incubated 3 min at 4°C in 25µl of a detergent buffer containing 0.2% Igepal CA-630 (Sigma, #I8896), 0.2% Tween 20 (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Cell Biology 2020Quote: ... Retinal organoids (n=3-4) for each time point were homogenized using a Dounce Tissue Grinder (Sigma-Aldrich, UK) and processed using SYBR™ Green Fast Advanced Cells-to-CT ™ Kit (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of purified LDL were mixed with 50 µL DiI stock solution (3 mg/mL in DMSO; Sigma) and incubated at 37℃ for 8 h ...
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bioRxiv - Cell Biology 2019Quote: ... glass-bottom dishes (IWAKI, Shizuoka, Japan) were treated with 2% acetic acid (WAKO) and 0.2% 3-(Trimethoxysilyl)propyl methacrylate (SIGMA) in 80% ethanol for 2 min ...
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bioRxiv - Cell Biology 2019Quote: ... Larvae were washed twice in E3 and wounded in a final 0.24 mg/mL tricaine (ethyl 3-aminobenzoate, Sigma)/E3 solution ...
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bioRxiv - Cell Biology 2020Quote: HBEC6-KT and Calu-3 cells were lysed using RIPA Lysis Buffer containing Protease Inhibitor Cocktail powder (Sigma-Aldrich) for 60-90 minutes at 4°C on a rocker ...
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bioRxiv - Immunology 2021Quote: ... 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium (BCIP /NBT) liquid substrates for AP-enzyme (SIGMA) were added for overnight incubation at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed and spots detected by adding a filtered solution of 0.2mg/mL AEC (3-amino-9-ethylcarbazole; Sigma) in 0.1M sodium acetate buffer [pH 5.0] and 0.05% hydrogen peroxide (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Abcam-17785-1-AP) and PSD-95 ((2.5 μg/ml diluted) Abcam-ab12093) using desired concentrations in PBS containing 3% BSA (Sigma), 0.3% Sodium azide (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by selection in 1 μg/mL (C4-2B) or 1.5 μg/mL (DU145, PC-3) of puromycin (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (ammonium salt) (PI(4,5)P2) (Sigma 850155P), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times and secondary staining was done in permeabilization buffer supplemented with 5% Donkey serum (Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... and Atto 647N-labeled 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (Atto 647N DPPE, ≤90.0%) were purchased from Sigma-Aldrich. Gibco™ Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated by pipetting (3 μl) or using a pinning tool (Sigma-Aldrich, St. Louis, MO, R2383-1EA) on control and selective media to assess specific phenotypes ...
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bioRxiv - Neuroscience 2021Quote: ... Then mice were randomly infused 10 minutes before the test with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
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bioRxiv - Molecular Biology 2021Quote: ... to 7.0 with HCl) for 45 minutes and a final wash in in 2x SSC (3 M NaCl (Sigma), 0.3 M sodium citrate (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: An RNA oligonucleotide (5’ -UUUUCAUGCUACGCGUAGUUUUCUACGCG- 3’) with Cyanine 5.5 at the 5’-end was obtained from Millipore Sigma (USA). Prior to the reaction ...
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bioRxiv - Cell Biology 2019Quote: ... the grid was washed twice for 3 minutes in washing solution containing filtered PBS and 0.2% glycine (Sigma-Aldrich), blocked for 30 minutes in filtered blocking solution containing ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed with PBS-T 3 times before the plates were incubated with 75μl Streptavidin peroxidase (1:1000, Sigma) for 60 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... The effect of GABABR antagonists on metamorphosis was tested using Phaclofen (10−3 M, 10−4 M, Sigma Aldrich) and the high affinity antagonist CGP-54626 (10−7 M ...
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bioRxiv - Systems Biology 2019Quote: ... Chk-2 inhibitor II BML-277 (used at 10 μM) from and Nutlin-3 (used at 0.75 - 4 μM, #N6287) from Sigma.
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bioRxiv - Cell Biology 2019Quote: ... Slides were washed 3 times in PBS for 5 min and incubated in DAPI (Sigma Aldrich, Cat. No.10236276001) for nuclei counterstaining ...