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Citations for Millipore Sigma :
9301 - 9350 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The cells were restreaked onto SD/-Trp/-Leu and incubated at 30 °C for 2–3 days before performing a colony-lift β-galactosidase assay (X-gal, Sigma-Aldrich) according to the Yeast Protocols Handbook (PT3024-1 ...
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bioRxiv - Cell Biology 2021Quote: ... Oocytes were surgically removed from anesthetized frogs (40, 46) under anesthesia (2 g/L Tricain, 3-aminobenzoic acid ethyl ester, Sigma A-5040). Preparation of defolliculated oocytes was carried out as described in (106) ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of freshly made 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 1 mg/mL; Sigma-Aldrich, St. Louis, MO) in serum-free ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared by layering supernatant gently on top of a discontinuous Percoll-density gradient (3% uppermost, 10% middle, and 23% bottom; Percoll, P1644, Sigma-Aldrich, UK) and centrifuged at 47,807 g for 8 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The surfaces were rendered hydrophilic or hydrophobic by vapor phase functionalization using 3-aminopropyltrimethoxy silane or chlorotrimethylsilane (Sigma Aldrich, St. Louis, MO), respectively ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were harvested during the exponential growth phase by centrifugation for 10 min at 10,000 g at room temperature with a Sigma 3-18K centrifuge (Sigma Aldrich, Seelze, Germany), washed with the same volume of fresh medium (without glucose or thiamine) ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
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bioRxiv - Microbiology 2019Quote: ... after which 0.5 mL of the top aqueous phase was removed and transferred to an Amicon 3 kDa molecular weight cutoff centrifuge filter (Sigma Aldrich, USA). Extracts were filtered by spinning in a benchtop centrifuge at 4 °C for 90 minutes at 13000g ...
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bioRxiv - Cancer Biology 2020Quote: ... It was mechanically dissociated using a scalpel in 1 ml of lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were re-suspended in 5 ml washing buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Cell Biology 2021Quote: ... and immersed into the oil phase (~125 mL) consisting of hexanes (99%) and 3% (v/v) Span 80 (Fluka, 85548 or Sigma-Aldrich, S6760). 10 mL of gel mixture was extruded through SPG membranes under nitrogen pressure of approximately 7 kPa ...
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bioRxiv - Cell Biology 2021Quote: ... up to 15 ml filtered supernatants were ultra-filtered through a 3 kDa MWCO Amicon® Ultra device (Merck-Millipore, MA, USA) by centrifugation at 4000 x g for 1 h at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... oocytes were incubated twice (1st 45 min, 2nd variable) in OR-2 containing 3 mg/ml collagenase type IV (Sigma, C-9891). Defolliculated oocytes were stored in ND96 solution containing (in mM) ...
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bioRxiv - Cell Biology 2020Quote: ... The liver tissue was cut into 3-4 mm pieces and incubated with 1 mM EGTA (Sigma-Aldrich, Cat. no. E0396-10G) in 1 x DBPS (Gibco™ ...
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bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
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bioRxiv - Plant Biology 2022Quote: ... one artificial plant (AP) having five leaves made of a Whatman filter paper (grade 237 equivalent to grade 3 CHR; Sigma-Aldrich, India) was also used as an inert control ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Immunology 2022Quote: ... Cultures were stimulated with 20 ng/ml PMA for 3 hours and assessed with a mouse IFN-γ ELISA kit (Sigma-Millipore). For airway epithelial cell culture ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were cultured in N2B27 containing either LIF and BMP4 (10 ng/ml, Stemgent 03-0007) or CHIR99021 (3 μM, Stemgent 04-0004-02) and PD0325901 (1 μM, Millipore Sigma 444968) respectively.
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed by sterile phosphate buffer saline (PBS) 3 times and then incubated with 5 mg/mL MTT (Sigma-Aldrich; #M2128) for 4 h ...
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bioRxiv - Bioengineering 2022Quote: ... were functionalized with O2 plasma (30 W / 30 s) followed by incubation in 1% v/v (3-Aminopropyl)triethoxysilane (APTES) (Sigma-Aldrich 440140) in water for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... or 3-week old mice (P21) were dissected to remove the skin and fat and stained with Alizarin Red (Sigma-Aldrich A5533) for visualization of the mineralized elements and Alcian Blue (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... was amplified from pBluescript II with SalI adapters using primers KL215 and KL216 and KOD Hot Start DNA polymerase (Millipore 71842-3). The PCR product was purified using a QIAquick PCR Purification Kit (Qiagen 28104) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from tendon-like constructs (n=4 animals, 3 constructs each) using TRI® Reagent (Sigma-Aldrich; Vienna, Austria) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices with Cav2-Cre injection at MPC and lENT were immunostained by Rabbit anti-Cre antibody (Millipore Sigma, Cat#69050-3) and Donkey anti-Rabbit secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... DS pupae were injected with either 3 μl of 2000 pg/μl (6000 pg total) (10% 20E in EtOH + 90% saline) of 20E (Sigma-Aldrich®) or with 3 μl of vehicle (10% EtOH and 90% saline ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then treated overnight in 1 volume of 100 % methanol and l/3 volumes of 100 % dichloromethane (DCM; Sigma-Aldrich; 270997). On the next day ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were typically seeded at a density of 1-3 × 105 cells/cm2 on dishes coated with poly-L-lysine (Sigma-Aldrich, P1524). Neuronal cultures were treated overnight in 1 μM tetrodotoxin (TTX ...
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bioRxiv - Immunology 2020Quote: ... and 24S-25,epoxycholesterol (10 µM, Enzo Cat# BML-GR231-0001, CAS: 77058-74-3) were compared to vehicle (ethanol, Sigma Cat# E7023). The UGCG inhibitor N-Butyldeoxynojirimycin (NB-DNJ ...
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bioRxiv - Microbiology 2021Quote: ... Three subsamples (∼2 ml) were taken from each section with a 3 ml syringe and immediately fixed in 2 ml RNAlater (Sigma Life Science) in a 5 ml tube (Qiagen ...
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bioRxiv - Bioengineering 2021Quote: ... Devices were first exposed to air plasma (50 W) and followed by filling channels with an aqueous solution of (3-Aminopropyl)triethoxysilane (APTES) (Sigma Aldrich, Germany). Devices were then incubated for 1 minute at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Patch pipettes with a tip resistance of 3 to 4 MΩ were filled with (in mM): K-gluconate (135 mM, Sigma; G4500-100G), EGTA (0.2 mM ...
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bioRxiv - Neuroscience 2022Quote: ... in addition to 10% FBS and 1% PS we supplemented the medium with 3 μg/ml puromycin (Sigma Aldrich, cat. no. P8833). For the experiments that involved transfection and electrophysiological recordings ...
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bioRxiv - Cell Biology 2020Quote: ... cleared in propylene oxide and embedded in a mixture of Epon 812 and Durcupan (Sigma; polymerization for 3 days at 60°C). Firstly ...
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bioRxiv - Molecular Biology 2020Quote: ... permeabilized with 0.5% (v/v) Triton X for 10 minutes and blocked for 30 minutes with PBS containing 3% goat serum (Sigma-Aldrich®), 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: Fabs (2-4 mg/ml) were mixed with a 2 to 3-fold molar excess of fen-G4 or fentanyl (Sigma F-013) in Dulbecco’s PBS and incubated for 1-2 hours at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Cancer Biology 2022Quote: ... Surgical specimens were washed twice in phosphate buffered saline (PBS) and fixed for approximately 3 hours in 4% paraformaldehyde (Sigma, St. Louis). Tissue was washed two times in PBS and incubated at 4°C for 3-5 days in 30% sucrose ...
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bioRxiv - Immunology 2019Quote: ... for 6 hours and then exposed to 0.5 × 105 red and 0.5 × 105 blue opsinised (1 hour in 10%v/v human serum) 3 μm diameter latex polystyrene beads (Sigma-Aldrich, Gilligham, UK) such that there were 1 bead per cell ...
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bioRxiv - Immunology 2019Quote: ... 24 and 48 hours and then fixed with 4°C methanol for 3 minutes and stained with safranin (Sigma-Aldrich, Gilligham, UK) for 10 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... gills were washed 3 ×20 min with phosphate-buffered saline (PBS) and then immunostained with anti-bromodeoxyuridine antibody (BrdU, Sigma-Aldrich, Inc.). Therefore ...
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bioRxiv - Evolutionary Biology 2019Quote: To measure sensitivity to perturbation of dopamine signaling we employed the startle response assay after pharmacological treatment with cocaine (Cocaine, COC-156-FB, Lipomed AG, Austria) or 3IY (3-Iodo-L-tyrosine, I8250, Sigma Aldrich, USA). Flies were reared for two generations in the high-temperature regime common garden experiment (Supplementary Figure S1) ...
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bioRxiv - Systems Biology 2019Quote: ... Cell chambers in the chip were filled with a 1:3 fibronectin (1 mg/mL) in sterile PBS solution (Sigma Aldrich, FC010), and incubated overnight at room temperature for thorough coating ...
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bioRxiv - Molecular Biology 2020Quote: ... permeabilized with 0.5% (v/v) Triton X for 10 minutes and blocked for 30 minutes with PBS containing 3% goat serum (Sigma-Aldrich®), 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... in serum was analyzed by measuring 3-hydroxybutyric acid (BOH) and acetoacetic acid (AcAc) using Ketone body assay kit (Sigma, No. MAK134) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2019Quote: ... permeabilization was achieved by flushing the chip for 3 h with PBST [PBS+ with 0.1% Tween-20 (P7949, Sigma-Aldrich Chemie GmbH)] ...
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bioRxiv - Biochemistry 2019Quote: ... All other chemicals including palmitate and 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) were obtained from Sigma-Aldrich (St. Louis, USA).
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bioRxiv - Molecular Biology 2019Quote: Immunoprecipitations were set up with 100 µl solubilised chromatin (roughly equivalent to 4 × 106 cells) and 3 µg of an anti-acetylated histone H3 antibody (Millipore, 06-599) in a total of 1 ml Native ChIP Incubation Buffer (10 mM Tris pH 7.5 ...