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Citations for Millipore Sigma :
9251 - 9300 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The membrane was incubated with the primary antibody for cleaved caspase-3 (ISIS, Cat# 9664) or β-Actin (Sigma, Cat# A-5060) for 2 h at room temperature or overnight at 4o C ...
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bioRxiv - Cell Biology 2020Quote: ... then rinsed 3 times in PBS (5 minutes each) and embedded in 4% low gelling temperature agarose in PBS (Sigma-Aldrich A0701). 55 μm slices of the ovaries were cut with a vibratome (Leica VT 1000 S ...
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bioRxiv - Cell Biology 2019Quote: ... cells were washed in kit Buffer A 3 times for 15 minutes and incubated with the PLA probes Duolink In Situ PLA Probe Anti-Mouse PLUS (Sigma-Aldrich, DUO92001) and Duolink In Situ PLA Probe Anti-Rabbit MINUS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed three times with PBS and blocked for 1 hour with 0.2% triton X-100 and 3% BSA (Sigma-Aldrich, Gillingham, UK) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated with PBS 1X supplemented with 3% Bovine Serum Albumin (BSA, cat. A1470-25G, Sigma Aldrich, St. Louis, MO, USA) to reduce non-specific reactions ...
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bioRxiv - Immunology 2021Quote: ... Cy3-STxB was generated by conjugating Cy3 dye onto STxB according to the manufacture manual of CY®3 Ab Kit (PA330000, Sigma-Aldrich).
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bioRxiv - Genetics 2021Quote: ... and then the second in IB-3 for further purification prior to a 100,000 × g spin through a Percoll (Sigma, cat. number P1644) gradient to separate MAMs from mitochondria ...
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bioRxiv - Genetics 2021Quote: ... Proteins were purified by Nickel-NTA resin and treated with TEV protease (1 mg lab made TEV per 40 mg of protein) and benzonase (100 units/ml, Novagen 70664-3) overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: Live zebrafish embryos and larvae were first anesthetized by bath application of 0.02% w/v solution of 1025 Ethyl-3-aminobenzoate methanesulfonate (Sigma-Aldrich, St. Louis) in filtered fish system water for 1 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We added 100 μl of cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 nM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630 (Sigma-Aldrich, St. Louis, MO)) and ground the cells with a pestle ...
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bioRxiv - Developmental Biology 2021Quote: ... vehicle control and treated (n=3 per group) were incubated in the sepeate fish tank containing dissolved BrdU (25 μg/L) (Sigma-Aldrich, USA) for 24 hours before tissue collection ...
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bioRxiv - Developmental Biology 2020Quote: Young-adult worms were randomly collected into 200 μl M9 solution and mixed with equal volume dyes (DiI, 1,1’-dioctadecyl-3,3,3’,3’,-tetramethylindo-carbocyanine perchlorate; Sigma-Aldrich, St. Louis, MO, USA) at working concentration (20 μg/ml) ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were washed 3×15min in PBS 0,01% Triton X-100 (PBST) and nuclei stained with DAPI (Sigma-Aldrich, #D9542, 1:10000) for 15min at RT after which they were washed once for 15min in PBST and 3×15min in PBS before mounting with Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then blocked in 3% BSA in PBS containing 0.2% Tween 20 before staining with rat anti-HA mAb 3F10 (Sigma, diluted 1:1,000) primary antibody in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: For RNA-binding protein immunoprecipitation (RIP) ~3×107 A549 cells for each sample were processed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μg of pSC2G-CFTR23 plasmid was co-transfected with 1 μM single-stranded DNA repair template (synthesized by Sigma, Table 3) comprising the CFTR-W1282X and silent protospacer adjacent motif (PAM ...
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bioRxiv - Immunology 2020Quote: ... For chromatin immunoprecipitation experiments cells were also treated with RXR agonist LG100268 (LG) (100 nM, Sigma Cat# SML0279, CAS:153559-76-3). For western blotting experiments cells were treated with LXR ligands for 48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... The RSCs from both mice were placed together and the tissue was cut into small pieces and transferred into a 1.5 ml microcentrifuge tube with 3 mg/ml isolation solution containing pronase (Sigma, Cat#P6911-1G), 1% BSA and 50 μg/ml DNaseI (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Wild-type ICR females (3-4 weeks old) were super-ovulated by sub-cutaneous injection of pregnant mare’s serum gonadotropin (PMSG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
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bioRxiv - Biochemistry 2019Quote: ... The fractions corresponding to the single peak were pooled and concentrated using an Amicon Ultra −15 centrifugal filter (3 kDa, Merck Millipore Ltd. Ireland). Copper-(I ...
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bioRxiv - Cell Biology 2019Quote: Single myofibers were isolated from the EDL muscles of 3 month Pax7-nGFP mice by digestion with collagenase I (Sigma, C-0130), as previously described (Wu et al. ...
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bioRxiv - Cell Biology 2020Quote: ... washed once with 5 mL ice-cold NETN250 buffer and lysed for 15 min at room temperature (RT) in ice-cold NETN250 buffer supplemented 5 mM MgCl2 and 125 U/mL benzonase (Novagen 71206-3). After addition of EDTA and EGTA to respective final concentrations of 5 mM and 2 mM to inactivate the benzonase and centrifugation for 30 min at 16,100 × g ...
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bioRxiv - Neuroscience 2019Quote: ... guinea pig anti-Synapsin 1 (1:500, SySy #106004), mouse anti-SMI-3 (1:2000, Covance #SMI-312R) and rabbit anti-GABA (1:1000, Sigma Aldrich #A2052). After washing with PBS ...
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bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... 10ug nuclear protein was incubated with dsDNA infrared tagged constructs (Table 1. 5’-3’ First strand, IDT, NSW, AUSTRALIA) with or without the additional supershift antiRAGE antibody (1ug, Millipore, CA, USA).
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bioRxiv - Microbiology 2019Quote: ... RNAs were extracted in 3 successive steps: i) extraction performed in Trizol reagent (Invitrogen TriReagent (Sigma-Aldrich, St-Louis MO 63103 USA)) ...
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bioRxiv - Neuroscience 2019Quote: ... BW723C86 (α-methyl-5-(2-thienylmethoxy)-1H-Indole-3-ethanamine monohydrochloride) and a primary antibody raised against β-actin were purchased from Sigma (USA). Other primary antibodies ...
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bioRxiv - Genetics 2019Quote: ... 59 nt spacer oligomers containing the 20 nt “spacer” and 19 nucleotides (5’ upstream, overlap to plasmid) and 20 nucleotides (3’ downstream, overlap to scaffold) were ordered (Sigma, Darmstadt, Germany). The different Cas9 vectors were linearized with restriction enzyme Acc65I ...
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bioRxiv - Microbiology 2019Quote: L929 cells in 6well plates were infected with T3D-PL or T3D-TD at an MOI of 3 and cell lysates were collected at 24hpi in TRI Reagent® (T9424, Millipore Sigma). Total RNA was purified using as per TRI Reagent® protocol ...
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bioRxiv - Immunology 2019Quote: ... blocked with 3% BSA and finally reacted with primary antibodies for phosphotyrosine (Cat No.: 05-321; clone: 4G10; EMD Millipore, Burlington, MA), DAP12 (Cell Signaling Technology) ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were next washed four times for 3 min in MilliQ water at room temperature and incubated in a 1% solution of thiocarbohydrazide (Sigma-Aldrich, USA) for 20 min at 60 °C ...
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bioRxiv - Genomics 2021Quote: ... A1413301)-coated 12-well plates at a density in the range of 3-7×105 cells per well in iPS-Brew + 2μM Thiazovivin (Sigma, cat. SML1045-5MG). Alternatively ...
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bioRxiv - Biophysics 2019Quote: ... was then re-suspended in 500 μL of a solution containing 90% PBS with 10% D2O with 3% TMS (Sigma Aldrich, 450510) and transferred to 5 mm NMR tubes ...
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bioRxiv - Genomics 2020Quote: ... 3 million CD4+ T cells were incubated in the presence of 5,6-Dichloro-1-β-D-ribofuranosylbenzimidazole (DRB, Sigma-Aldrich, Cat: D1916) at a final concentration of 65μM or Triptolide (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... The tissue was mechanically lysed with a scalpel in a total volume of 5 ml lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Biochemistry 2021Quote: ... 500 µL filter sterilized supernatant was concentrated to 100 µL using Amicon ultra centrifugal filter units with 3 000 Da cut-off (Merck Millipore, Cork Ireland) and washed three times in 10 mM Tris pH 7.5 ...
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bioRxiv - Synthetic Biology 2021Quote: To induce the degradation of mAID-fused proteins, cells were treated with 500 µM indole-3-acetic acid (auxin, IAA, dissolved 500 mM in water) (Millipore Sigma, I5148) for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with a 1:3 molar ratio of heavy chain and light chain expression vectors using the Expi293 Transfection kit (Sigma-Aldrich; L3287) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared for NMR by combining 16 µL standard with 50 µL serum and placing in a 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... Individuals were euthanized with a 0.1% solution of ethyl 3-aminobenzoate methane sulfonate (MS-222; Sigma, St. Louis, MO, USA; A-5040). The brains were removed and fixed by immersion in 4% w/v paraformaldehyde in 0.1 M phosphate buffer (PB ...
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bioRxiv - Biophysics 2020Quote: ... Wet chemical etching solution for Au: 20 mM Fe(NO3)3·9 H2O (Fluka, Switzerland), 30 mM thiourea (Fluka, Switzerland) and 1 mM HCl (Sigma-Aldrich, USA) dissolved in ultrapure water saturated with octanol (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... Devices were first exposed to air plasma (50 W) followed by filling channels with an aqueous solution of (3-Aminopropyl)triethoxysilane (APTES) (Sigma Aldrich, Germany). Devices were then incubated for 1 min at RT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at a concentration of 1.5 mM was sonicated in nanopore water with 3 mM lactic acid (Sigma-Aldrich, L1750, St. Louis, MO) and sterilized via passage through a 0.2 μm filter as described in (5) ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were lysed and the ATP content was measured by an ATP assay kit per manufacturer’s instruction (Millipore Sigma, n = 3).
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed with PBST 2-3 times and bands were visualized upon addition of SigmaFast BCIP®/NBT tablets (Sigma B5655) (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue from three rats per sex was combined and transferred to 5 ml of ice cold lysis buffer (10 mM tris-HCl, 10 mM NaCl, 3 mM MgCl2 and 0.1% Igepal in nuclease-free water (Sigma-Aldrich, 18896-50ML) for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies], 2 µg/mL heparin [Sigma-Aldrich] ...
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bioRxiv - Biophysics 2020Quote: ... An emulsion was generated by injecting 50 μL of pre-bead-solution in 500 μL of n-hexane with 3% Span®80 (Sigma-Aldrich) using a 100 μL Hamilton Syringe ...
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bioRxiv - Plant Biology 2021Quote: ... and 8 DAP and cleared in a chloral hydrate solution (chloral hydrate, Sigma C8383/Glycerol/Water, 8/1/3 w/v/v) for embryo phenotyping ...