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Citations for Millipore Sigma :
9001 - 9050 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Ovarian cells were isolated by enzymatic digestion in 3 mL of Dulbecco’s Modified Eagle’s Medium (DMEM) (cat# D6429, Sigma) containing 4 mg collagenase (cat# C5138-100MG ...
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bioRxiv - Genetics 2023Quote: ... at 37 °C on a heating plate with 10µL digestion mix (3% [wt/vol] pectolyase Y-23 [MPBiomedicals], 0.3% [wt/vol] Driselase [Sigma] 0.3% [wt/vol] cellulase [OnozukaR10] [Duchefa] 0.1% sodium azide in 10 mM citrate buffer) ...
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bioRxiv - Genomics 2023Quote: ... Young adult worms were harvested into 100∼200 μL M9 buffer and mixed with equal volume dyes (DiI, 1,1’-dioctadecyl-3,3,3’,3’,-tetramethylindo-carbocyanine perchlorate, Sigma, 468495) at the working concentration (20 μg/ml) ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were washed 3 times in PBS before addition of 2% Triton X– 100 (Sigma-Aldrich) PBS solution to lyse the cells for enumeration of the intracellular bacteria ...
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bioRxiv - Cell Biology 2023Quote: ... Imidazole was removed by 3 cycles of concentration and dilution using Vivaspin (Millipore Sigma, Cat# GE28-9322-25). TEV enzyme was added at about 1/10 of the target protein mass and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... was washed with the IP lysis buffer and incubated with 3 ug anti-Flag antibody (Sigma, M2, F1804) for 1 h at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... antisense strand: 5’-GUA UCU CUU CAU AGC CUU A(dT)(dT)-3’) were synthesized by Sigma Aldrich. Early Endosome Antigen 1 (EEA1 ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were washed in PBS and permeabilised in PBS supplemented with 3% FBS and 0.1% Saponin (Sigma-Aldrich) for 5 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: The following chemicals were used in this study: indole-3-acetic acid (IAA, auxin) (87-51-4) (Millipore); 4-hydroxytamoxifen (4-OHT ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Developmental Biology 2024Quote: ... Stained zebrafish larvae were anesthetized with 0.016% (m/v) Ethyl 3-aminobenzoate methanesulfonate salt (Tricaine; Sigma-Aldrich, A5040) and embedded in 1.8% low melting point agarose (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and collagenase type 1 was purchased from Sigma-Aldrich. RNA extraction kit (Iran) ...
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bioRxiv - Cell Biology 2024Quote: Treatments for the LC3 assay included 3 hours with mTOR inhibitor Torin1 (Merck-Millipore, final concentration 250 nM), Bafilomycin A1 (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... After 24 h media/inhibitor was aspirated and replaced with 20 μl of 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) and incubated at 37°C in 5% CO2 for 3 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Bands were stained in 5-bromo-4-chloro-3-indolyl-phosphate and nitro blue tetrazolium solution (Sigma-Aldrich). The membrane image was acquired using a digital steel camera EOS M6 mark II with a lens EF16-35 mm F4L IS USM (Canon Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... slides were cleared in Xylene (2 × 3 min) and coverslipped with DPX mounting medium (Cat# 44581, Sigma-Aldrich). Sections were photographed with an epifluorescence microscope (Model BX51 ...
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bioRxiv - Cell Biology 2024Quote: ... The number of viable cells was quantified using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; M2128, Sigma) assay at 0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total of 150 µL of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone; Sigma-Aldrich) dissolved in 100% ethanol (Wako ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was applied to 3 kDa MWCO filters (Amicon Ultra-0.5 Centrifugal Filter; Merck Millipore, Darmstadt, Germany), and centrifuged at 14,000 × g for 45 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 min at RT and then permeabilized for 3 min with 0.2% Triton-X 100 (Millipore Sigma) Over-extracted cells were treated with 0.4% Triton-X 100 ...
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bioRxiv - Cancer Biology 2024Quote: ... the PDMS stamp is placed on a silanized coverslip pretreated with 2% (3-Trimethoxysil) propyl-methacrylate (Sigma #M6514)) ...
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bioRxiv - Cancer Biology 2024Quote: ... corrected for saturation and normalized to an external reference (sodium 3-(trimethylsilyl) propionate-2,2,3,3-d4 (TSP; Sigma-Aldrich)) and to cell number (64).
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bioRxiv - Microbiology 2024Quote: ... They were washed three times with PBS and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich, A3294) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... the animal’s drinking water was replaced from days 3-8 with water containing 5% L-arabinose (Sigma Aldrich). InducTn-seq samples were collected 8 days post inoculation by plating fresh feces on solid LB containing 50 µg/ml kanamycin ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: PESTANAL® analytical grade tefluthrin (2,3,5,6-tetrafluoro-4-methylbenzyl 3-[(1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl]-2,2-dimethylcyclopropanecarboxylic acid) was purchased from Sigma Aldrich. Tefluthrin stock solutions were prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2024Quote: ... a collagen raft mixture was prepared on ice via combining 3 mg/ml collagen (Millipore-Sigma, #08-115) with 10X E-Media (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... a collagen raft mixture was prepared on ice via combining 3 mg/ml collagen (Millipore-Sigma, #08-115) with 10X E-Media (DMEM ...
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bioRxiv - Immunology 2024Quote: 1.5 x 106 YTS NK cells were cultured in 3 mL of media containing 1.8 mL of previously obtained viral media and 8μg/mL polybrene (Sigma). The culture plates were spun for 2 hours at 400 x g and then placed in an incubator overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 10 μM of the agonist N-[(3R)-1-Azabiciclo[2.2.2]oct-3-yl]furo[2,3-c]pyridine-5-carboxamide (PHA-543613, from Sigma, PZ0135) for 30 min ...
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bioRxiv - Genetics 2024Quote: Adult zebrafish (3 months post fertilization) were euthanized using a 25x tricaine solution (4000 mg tricaine powder (Sigma), 979 ml distilled water ...
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bioRxiv - Cancer Biology 2024Quote: ... 3-(2-Methyl-5-nitro-imidazol-1-yl)-N-(2,2,2-trichloro-1-phenylamino-ethyl)-propionamide) (Sigma-Aldrich SML1503). Drugs were diluted in culture media at treatment.
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bioRxiv - Neuroscience 2024Quote: ... They were maintained in a Petri dish containing embryo medium complemented with 3% Methylcellulose (Sigma-Aldrich, USA, #274429) to allow dorsal and lateral pictures of the whole body on glass microscope slides ...
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bioRxiv - Genetics 2021Quote: ... Then human 293T cells were transduced with lentiviral particles of plkoSCR (Mission plko.1 puro; SHC002) or shCNBP human (Mission plko.1 puro TRCN0000311158, SIGMA-ALDRICH) at a MOI=5 for 72h ...
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bioRxiv - Pathology 2021Quote: Hydrogels (n=6/time point) were immersed in a solution of fluorescein-conjugated human IgG (IgG-FITC, final concentration 1 mg/mL) (Sigma-Aldrich) in TBS containing 0.1% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... and 1x non-essential amino acid) and 50 ng/mL of human macrophage colony-stimulating factor (M-CSF, Millipore, Temecula, CA) for 5-6 days at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were maintained in vitro in human O+ erythrocytes at 4% haematocrit in RPMI 1640 medium supplemented with 25mM HEPES (Sigma-Aldrich), 0.25% sodium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: Human HEK293T cell line was grown in Dulbecco’s Modified Eagle’s Medium containing high glucose with HEPES modification (Sigma-Aldrich®, D6171), supplemented with 10% fetal bovine serum (PAN Biotech ...
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bioRxiv - Molecular Biology 2021Quote: Murine or human differentiated brown adipocytes were fixed with 4% PFA and stained with 0.3% Oil Red O solution (Sigma-Aldrich, O0625). The images were observed under a Nikon microscope.
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bioRxiv - Biochemistry 2020Quote: ... The following compounds were selected from the “in-human” collection docking screen and purchased from different vendors: Pterin (Sigma-Aldrich, P1132), Verdiperstat (MedChem Express ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed six times and incubated with 100 μL/well horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Fc-specific, Sigma Aldrich) diluted 1/5000 in PTA buffer ...
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bioRxiv - Immunology 2019Quote: Blood derived from healthy human volunteers was collected into citrate tubes (Becton Dickinson) and layered into Ficoll-Paque Plus (Sigma-Aldrich). Samples were centrifuged at 1440 rpm in a swinging bucket centrifuge for 20 min at 23°C without braking ...
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bioRxiv - Biochemistry 2019Quote: ... Cohn fraction IV (Product # G3637) and human whole serum (sterile-filtered from male AB plasma, Product # H4522) were purchased from Sigma-Aldrich, St ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Immunology 2019Quote: ... Cytokine levels in restimulated T cell cultures and SLE patient plasma samples was measured using Milliplex® MAG Human Cytokine/Chemokine Immunoassays (Millipore).
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bioRxiv - Neuroscience 2019Quote: ... transverse paraffin sections of spinal cord mounted on positive charged glass slides were processed for double immunohistochemistry with specific antibodies for human neural stem cells (Sigma-Aldrich) and examined under a fluorescence microscope ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with PBST and 100 μl/well of goat anti-human immunoglobulins-peroxidase IgG (FC specific; Sigma-Aldrich), IgM (μ-chain specific ...
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bioRxiv - Immunology 2019Quote: ... PerioPaper strips were centrifuged at 3000 × g for 15 min at 4°C in PBS elution for the dosage of the tissue destruction and bone resorption markers by Multiplex (Human MAP, Millipore, USA).
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bioRxiv - Biochemistry 2019Quote: ... and eluted by competitive elution in Flag Wash Buffer supplemented to 200 ug/mL Flag peptide and 250 ug/mL human insulin as a carrier (Sigma-Aldrich). The protein was then passed over a 2 mL amylose column (NEB ...