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Citations for Millipore Sigma :
8901 - 8950 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Roots were then squashed under coverslips onto slides that had been pre-treated with 3-Aminopropylthiethoxysilane (Sigma-Aldrich) to increase tissue adherence (4) ...
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bioRxiv - Microbiology 2022Quote: ... They were then subjected to bead beating (3 × 45 s) with glass beads (particle size ≤ 106 μm; Sigma) using a MP Biomedicals™ FastPrep-24™ (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Peroxidase activity was revealed by incubating slides in 3-amino-9-ethylcarbazole (AEC) (Sigma, St Louis, MO, USA) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... N-(3-aminopropyl)methacrylamide hydrochloride (APMA) and N,N′-bis(acryloyl)cystamine (BACA) were purchased from Sigma-Aldrich. Peptides ...
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bioRxiv - Molecular Biology 2023Quote: ... Blocking and antibody dilution was done in 3% bovine serum albumin (BSA) solution in DPBS (Sigma-Aldrich #A7979), 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS and 10% 5mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (SIGMA) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... we delivered the Iκ-kinase inhibitor [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide (TPCA-1; Sigma-Aldrich CAS 507475-17-4 ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioning was performed on groups of around 40-50 flies with 3-octanol (around 95 % purity; Sigma-Aldrich) and 4-methylcyclohexanol (99 % purity ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were enzymatically dissociated for 20 min at 37°C with papain (3 U/mL, Sigma-Aldrich), diluted in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Physiology 2023Quote: ... Samples were separated on a SeQuant ZIC-pHILIC column (100 3 2.1 mm, 5 mm, polymer, Merck-Millipore) including a ZIC-pHILIC guard column (2.1 mm x 20 mm ...
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bioRxiv - Microbiology 2022Quote: ... female 3–4-week-old BALB/c or C57BL/6 mice were injected intraperitoneally with 10mg azoxymethane (Sigma) per kg mouse weight ...
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bioRxiv - Neuroscience 2022Quote: ... AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptors were blocked using 6,7-dinitroquinoxaline-2,3-dione (DNQX, Sigma) dissolved in dimethyl sulfoxide and diluted by 1000 in aCSF for 10μM bath application.
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bioRxiv - Neuroscience 2022Quote: ... Slides were blocked by incubation in 3% bovine serum albumin (with 0.5% Triton X-100 dissolved in 0.1 M PB, Sigma) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... or MG132 using a MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-based in vitro toxicology kit (TOX1-1KT, Sigma). 10 µl MTT solution was applied to cells at the end point of the experiment and incubated for 4 hours ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed using 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) (Sigma Aldrich, 11697471001 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
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bioRxiv - Plant Biology 2022Quote: ... the same total protein extracts were incubated with Endoglycosidase H at 37 °C for 3 h (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... or 10nM GIP in stimulation buffer (1x Hanks Buffered Saline Solution, 0.1% BSA, 0.1% 3-isobutyl-1-methylxanthine (IBMX) (all Sigma), 0.5mM HEPES (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Pooled cells from 3 experimental replicates per group were lysed in lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... spheroids were incubated with 0.5 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) for 2-3 h until fully stained ...
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bioRxiv - Neuroscience 2024Quote: D1R agonist 2,3,4,5,-tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine hydrochloride (SKF38393; Sigma-Aldrich, St Louis, MO) was dissolved in sterile saline at a concentration of 0.5 μg/μL ...
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bioRxiv - Bioengineering 2023Quote: ... 1000 μM) prepared from a 0.5 M stock solution of 3-MB (Sigma-Aldrich, St Louis, MO, USA) solubilized in a 0.5 M NaOH solution and filtered through a 0.2 μm filter for sterilization (Minisart 0.2 μm syringe filter ...
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bioRxiv - Plant Biology 2024Quote: Leaves of 3-4-week-old Nicotiana benthamiana plants were agroinfiltrated and sprayed with 10 μM estradiol (Sigma) 24 h after infiltration to induce protein expression ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were extensively washed in lysis buffer and elution was carried out with 3×FLAG peptide (Sigma-Aldrich), HA peptide (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... SidK(1-278)-3×FLAG (∼0.85 mg) was bound to 1.5 mL of M2-FLAG affinity gel resin (Sigma) and flow through was recirculated onto the column 6-7 times ...
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bioRxiv - Cell Biology 2024Quote: ... in 3% (w/v) BSA were added for 1 hour before addition of 300 nM DAPI (Sigma Aldrich) and ProLong® Gold Antifade (Cell Signaling Technology) ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, St Louis, MO, USA) was added to selected wells and cells were incubated at 37°C for 4h ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed with 0.5% glutaraldehyde and incubated for 3 min at RT with 5 mg/ml sodium borohydride (Sigma). After washes ...
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bioRxiv - Physiology 2024Quote: ... electrode tips were dipped in a solution of polyvinyl-chloride in tetrahydrofuran (10 mg in 3 mL; Sigma) to prevent ionophore displacement ...
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bioRxiv - Molecular Biology 2024Quote: ... A 100 mg tablet of 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, Sigma A9941) was dissolved in 100 mL of citrate buffer (50 mM ...
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bioRxiv - Neuroscience 2024Quote: Larval zebrafish were mounted at 5 dpf in 3% low melting point agarose (Sigma-Aldrich, St. Louis, Missouri) on top of a 22x22 mm coverslip ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were blocked and permeabilized using 3% bovine serum albumin (BSA) and 0.25% Triton X-100 (Sigma Aldrich) in PBS for 30 minutes at RT ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μL MTT (5 mg/ml tetrazolium salt 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Sigma-Aldrich) was added to each well and kept in a dark for 4 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 min at RT and then permeabilized for 3 minutes with 0.2% Triton-X 100 (Millipore Sigma). Over-extracted cells were treated with 0.4% Triton-X 100 for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... anesthetized in E3 medium with 0.016% (w/v) buffered tricaine (3-amino benzoic acid ethyl ester; Sigma-Aldrich), and embedded in 0.8% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... They were then washed 2x with PBS and blocked using 3% bovine serum albumin (BSA, A2058, Sigma-Aldrich) in PBS for 1h at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... PFA was removed and samples were washed 3 times with PBST (RNAse-free PBS and Tween20, Sigma Aldrich) with incubation times of 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... the medium was removed and replaced with 350 μl of fresh medium containing 3 μM ARL67156 (Sigma, A265). At the relevant experimental time points ...
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bioRxiv - Bioengineering 2024Quote: ... have been colored with either Solvent Yellow 7 or with Solvent Green 3 (Sigma-Aldrich, St. Louis, MO) at concentrations of 0.50 mg/mL and 1.43 mg/mL respectively ...
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bioRxiv - Biochemistry 2023Quote: ... and phosphatase inhibitors (prepared in-house according to Phosphatase inhibitor cocktail 1, 2 and 3 from Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies against total cABL (Ab-3) (cat. OP20) and total RAS (cat. 05-0516) were purchased from Millipore. Antibody for GAPDH (cat ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in the presence or absence of aphidicolin 2μM for 3 hours together with cytochalasin B (3μg/ml, Merck Millipore) to block cytokinesis prior to fixation for immunofluorescence.
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bioRxiv - Systems Biology 2023Quote: ... 10 mL of culture was centrifuged at 3800 x g for 15 min (SIGMA 3-16KL refrigerated centrifuge). The bacterial pellets were harvested ...
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bioRxiv - Microbiology 2023Quote: ... At 2 hrs and 24 hrs post-infection (p.i.) the BMMs were rinsed 3 times with sterile PBS and lysed in PBS/0.1% sodium deoxycholate (Sigma), followed by serial dilution in sterile PBS and plating on TSAC plates for CFU enumeration ...
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bioRxiv - Developmental Biology 2022Quote: ... oocytes were cleaned of cumulus cells by constant pipetting for 3-minutes in vortex in 100µl drop of TL HEPES with 0.05% Hyaluronidase (Sigma), washed in TL HEPES ...
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bioRxiv - Immunology 2023Quote: ... the plates were washed 3 times and the reaction was developed with 100uL p-Nitrophenyl phosphate (PNPP, Sigma; N-9389 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then stripped and re-probed with an 18s rRNA targeting probe (5’-CCATCCAATCGGTAGTAGCG-3’, Sigma) which was used for signal normalisation.
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bioRxiv - Microbiology 2023Quote: ... and then blocked with blocking buffer solution (PBS/0.05% Tween 20 /3% non-fat milk (AmericanBio) /0.5% Bovine Serum Albumin (Sigma)) at room temperature for 2 hours ...
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bioRxiv - Genetics 2023Quote: ... Supernatant was then transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, no. UFC500396) and centrifuged for 45 min at 4 °C ...