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Citations for Millipore Sigma :
8801 - 8850 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Adult mice (3-4 months old) were injected intraperitoneally (i.p.) with 100 mg/kg of tamoxifen (Sigma-Aldrich) dissolved in 10% EtOH in corn oil (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Foam formation was avoided by the addition of 3 % (v/v) antifoam 289 (Sigma-Aldrich; St. Louis, MO). Samples were withdrawn throughout the fermentation process with the purpose of evaluating the biomass and recombinant protein expression.
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bioRxiv - Neuroscience 2020Quote: ... CGNs (3 × 106 cells) and plasmids were suspended in 70 μl of Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) and exposed to decay pulses using a CUY21-edit II pulse generator (BEX ...
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bioRxiv - Immunology 2021Quote: ... Subsequently cells were washed 3 times with PBS before being treated with 2% saponin (250 µL/well) (Sigma) in distilled water and incubated for 15 min at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately1-2 μL of endotoxin-free DNA (1-3 mg/ml) diluted in PBS/0.025% Fast Green (SIGMA) was injected into the lateral ventricles of the forebrain using heat-pulled glass micropipettes (Drummond) ...
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bioRxiv - Cancer Biology 2020Quote: ... Metal-loaded polymers were then washed with L-buffer and concentrated on a 3 kDa Amicon filter (Millipore). Partially reduced antibodies were incubated with metal-loaded polymers for 90 min at RT followed by washing with W-buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cell viability was evaluated by using the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell proliferation assay (Sigma). Non-treated cells were used as control ...
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bioRxiv - Microbiology 2022Quote: ... LAM was resuspended at 1 mg/mL in sterile nanopure water (Direct-Q 3 UV-R, Millipore Sigma). 60-μL aliquots were stored in low-retention tubes at –80 °C and thawed immediately prior to use ...
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bioRxiv - Microbiology 2022Quote: ... stained with REVERT total protein stain (Licor Biosciences) and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ~25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Bioengineering 2022Quote: ... Bis-cysteine crosslinking peptide VPM (GCNSVPMSMRGGSNCG) or non-degradable crosslinking agent DTT (1,4-dithiothreitol; Sigma, 3483-12-3) was dissolved in diH2O at 27.3 mM ...
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bioRxiv - Microbiology 2022Quote: ... 3-OH FAs were quantified by integrating the appropriate peak on the ion chromatogram and comparing the area with an analogous deuterated internal standard (3- hydroxytetradecanoic acid, 2,2,3,4,4-d5; Sigma-Aldrich, France). The internal standard (0.5 mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were then washed in PBTD for 3×10 min and 1mg/ml DAPI (1:250, Sigma-Aldrich) was added and incubated for 10 minutes between the first and second washes ...
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Biophysics 2019Quote: Zebrafish were anaesthetized by immersion in Ethyl 3-aminobenzoate methanesulfonate (0.02% in PBS, pH 7.5, Sigma-Aldrich, A5040) until respiratory movements of the opercula stopped (approximately 5 min) ...
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bioRxiv - Neuroscience 2019Quote: Zebrafish larvae were anesthetized in 0.02% w/v ethyl 3-amino-benzoate methanesulfonic acid (MS-222; Sigma-Aldrich), placed in a glass-bottomed dish ...
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bioRxiv - Neuroscience 2019Quote: Gaboxadol (4,5,6,7-retrahydroisoxazolo[5,4-c]pyridin-3-ol hydrochloride, THIP hydrochloride; Sigma Aldrich, St. Louis, MO, USA, # T101) was dissolved in dH2O at 1 mg/ml ...
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bioRxiv - Physiology 2019Quote: ... Differentiated osteoclasts with >3 nuclei were identified as tartrate-resistant acid phosphatase-positive (Leukocyte Acid Phosphatase Assay, Sigma).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... fish were immersed in fresh Tricaine (0.016%w/v; 3-amino benzoic acid ethyl ester; Sigma A-5040) in system water buffered with NaHCO3 to pH 7.5 until motionless ...
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bioRxiv - Cell Biology 2019Quote: ... we replaced media with either 400 uM mimosine + 500 uM indole-3-acetic acid (auxin; Sigma-Aldrich I5148) supplemented media to degrade RAD21 or 400 uM mimosine-supplemented media alone as an untreated control ...
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bioRxiv - Molecular Biology 2019Quote: ... Aortas were briefly treated for 30 minutes at 37°C with 3 mg/ml collagenase (Sigma C-0130) to help in cleaning away the adventitia ...
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bioRxiv - Biophysics 2019Quote: ... cells were incubated with 0.5 mg/ml 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, Sigma-Aldrich) for 2 hours at 37 °C ...
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bioRxiv - Microbiology 2019Quote: Malt extract media (MEM) agar was prepared as 3 % malt extract (Roth) and 0.5 % peptone from casein (Sigma) in 1.5 % agar-agar (Sigma) ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were developed using nitroblue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP) (Sigma) in staining buffer (100 mM Tris pH9.5 ...
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bioRxiv - Developmental Biology 2019Quote: Neurospheres at passage 3 were dissociated and plated at 10,000 cells/well in Poly-L-ornithine (Sigma, P3655) and Laminin (Millipore CC095 ...
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bioRxiv - Developmental Biology 2020Quote: ... containing pre-equilibrated preimplantation embryo culture media supplemented with 1 µM PDO325901 and 3 µM CHIR99021 (Sigma-Aldrich). Embryos were grown one more day in NDiff 227 media (Takara ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 6 mg RBD protein in 3 mL PBS were mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the first boosting ...
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bioRxiv - Cell Biology 2021Quote: To measure symplasmic connectivity using the HPTS (8-Hydroxypyrene-1, 3, 6-trisulfonic acid trisodium salt, SIGMA-ALDRICH) dye movement assay ...
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bioRxiv - Neuroscience 2020Quote: ... The recording chamber was constantly perfused (2-3 ml/min) with aCSF and 100 μM picrotoxin (Sigma Aldrich) was added to block inhibitory synaptic transmission ...
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bioRxiv - Neuroscience 2020Quote: ... and cargo in a 1:4:1 ratio with PEI reagent (1:3 plasmid to PEI ratio, Sigma). Three days after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 µL of sample was carefully spotted onto pre-washed PEI-cellulose TLC plates (Merck Millipore, #105725). The TLC plate was eluted in developing buffer containing 10 mM NH4Cl and 5% acetic acid for 100-140 min ...
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bioRxiv - Neuroscience 2019Quote: ... washed 3×10 min in PBS-B and developed in diaminobenzidine tablets (DAB) (cat# D4418-50 SET; Sigma) for 3 min.
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bioRxiv - Microbiology 2019Quote: ... multi-tube mixer for 3 min before adding one volume of phenol:chloroform:isoamyl alcohol (25:24:1) (Sigma-Aldrich). Samples were mixed vigorously ...
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bioRxiv - Microbiology 2020Quote: ... and 200 μM of a non-selective family-wide phosphodiesterase inhibitor 3-Isobutyl-1-methylxanthine (IBMX; Sigma, #I5879) in a mix of DMEM/F-12 medium (50/50 ...
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bioRxiv - Microbiology 2020Quote: ... Horseradish peroxidase activity was revealed by incubating slides in 3-amino-9-ethylcarbazole (Sigma, St Louis, MO, USA) solution ...
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bioRxiv - Neuroscience 2019Quote: ... Protein lysate was isolated in RIPA buffer supplemented with a protease and phosphatase-3 inhibitor cocktail (Sigma-Aldrich). Lysate (20 μg ...
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bioRxiv - Neuroscience 2020Quote: To label endogenous RNAs neurons were washed once with cold PBS with 3 μg.ml-1 digitonin (Sigma Aldrich), once with 50% methanol in PBS and fixed in cold 100% methanol for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... except that subjects received counterbalanced ICV infusions (3 uL) of either vehicle [2% DMSO (Sigma-Aldrich, D8418-50ML) + 10% 2-hydroxypropyl-β-cyclodextrin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed 3x in TBS then blocked for endogenous peroxidase activity by incubation in 3% H2O2 (Sigma) diluted in methanol for 10 min at 4°C ...
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bioRxiv - Pathology 2021Quote: ... The supernatants were concentrated with an Amicon Ultra 3 kDa nominal molecular weight limit centrifugal filter unit (Millipore), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Dechorionated larvae were anesthetized with 0.02% (w/v) ethyl 3-aminobenzoate methanesulfonate (MS-222) (Tricaine, Sigma-Aldrich, A5040) supplemented with 0.2mM PTU ...
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bioRxiv - Immunology 2021Quote: ... in 3% BSA-containing DPBS for 15 min at RT and stained with phalloidin-TRITC (P1951, Sigma Aldrich) and DAPI (D9542 ...
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bioRxiv - Immunology 2020Quote: ... Plaques were then visualised by incubating the cells with ExtrAvidin peroxidase followed by 3 amino-ethylcarbazole substrate (Sigma).
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bioRxiv - Biophysics 2019Quote: ... Coverslips were rinsed with distilled water and then incubated in 0.5% 3-Aminopropyltriethoxysilane (APTES) (Sigma, 919-30-2) in 95% ethanol ...
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bioRxiv - Developmental Biology 2019Quote: ... and this sequence was amplified by PCR from 3’ RACE cDNA using KOD Hot Start Master Mix (Novagen) with the following primers ...
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bioRxiv - Immunology 2019Quote: ... 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) was obtained from Sigma-Aldrich (Saint Louis, MO, USA). FcR blocking reagent (BR ...
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bioRxiv - Cancer Biology 2020Quote: ... the samples were then concentrated with a 3 K cutoff centrifugal filter device (cat No. UFC500396, Millipore, USA) and stored at -80 °C until further analysis.
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bioRxiv - Immunology 2019Quote: ... we located the orifice of the Wharton’s duct in the sublingual caruncle and inserted a pointed glass-capillary (Untreated Fused Silica Tubing - L × I.D. 3 m × 0.10 mm, #25715, Sigma). The glass capillary was connected to a Hamilton Micro-syringe (Hamilton ...
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bioRxiv - Physiology 2019Quote: ... ESFs were decidualized by adding of 0.5 mM 8-bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP) (Sigma) and 0.5 μM of the synthetic progestin medroxyprogesterone acetate (MPA ...
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bioRxiv - Genomics 2021Quote: ... Cross-linked chromatin was immunoprecipitated with either 5 μg anti-TCF4/TCF7L2 (Clone 6H5-3, #05-511, Millipore) or normal mouse control IgG (#12-371 ...