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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... No. H7379) and human hemoglobin A0 (HbA0) (with 5% (w/w) methemoglobin) (Cat. No. H0267) (all protein samples from Sigma, USA). Tetrahydrofuran (THF ...
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bioRxiv - Immunology 2024Quote: Luminex assays detecting soluble ICAM-1 (sICAM1) were conducted using the MILLIPLEX Human Sepsis Magnetic Bead Panel 1 Kit (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Tween-20 in PBS for 15 min at RT and then incubated with rabbit anti-human RYBP polyclonal antibody (Sigma, HPA053357) and mouse anti-human hCG-β antibody (abcam ...
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bioRxiv - Immunology 2024Quote: ... The wells were rinsed three times with PBS containing 0.05 % Tween 20 and horseradish-peroxidase-labeled rabbit anti-human or anti mouse IgG (100 μL/well; Sigma-Aldrich, Germany) was added to each well and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... 5 U thrombin increments were added to microcentrifuge tubes containing 50 µl of human citrated plasma (Sigma Cat No. P9523-1ML), the time to clot formation was recorded ...
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bioRxiv - Bioengineering 2024Quote: To test the micromechanical properties of meniscal cells and newly synthesized PCM, 35-mm petri dish (351008, Life Sciences) was treated with 20 µg/mL human fibronectin (10838039001, Sigma-Aldrich) for 12 h prior to seeding meniscal cells with an initial density of 5,000 cells per dish ...
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bioRxiv - Microbiology 2024Quote: ... by human neutrophils was measured over a time of 30 minutes by monitoring luminol-amplified chemiluminescence using 282 μM luminol (Sigma Aldrich). Neutrophils were stimulated either with negative control (Hank Balanced Salt Solution ...
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bioRxiv - Cell Biology 2024Quote: ... The shRNA plasmids were obtained from the human genome-wide TRC shRNA library (purchased from Sigma-Aldrich, USA, Catalog no. SH0411), available at shRNA resource centre ...
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bioRxiv - Neuroscience 2024Quote: Proteins from human cerebral cortex and whole mouse brain were extracted using RIPA buffer (MilliporeSigma) with Protease Inhibitor cocktail (Sigma P8340) as previously described [20] ...
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bioRxiv - Cell Biology 2024Quote: ... For transfection of human or monkey keratinocytes tissue culture plastic vessels were coated with 20 µg/ml rat Collagen I (Sigma, C3867) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... treated 3T3 mouse fibroblast feeder cells and grown until 70% confluency in the 3:1 mixture of Ham’s F12/DMEM media (HyClone) supplemented with 5% FBS (Sigma), 24 μg/mL adenine (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions from size exclusion column were pooled and concentrated using 3 kD cutoff ultracentrifugal device (Millipore, USA). The EspC purity was checked on 12% SDS-PAGE and by mass spectrometry ...
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bioRxiv - Cancer Biology 2021Quote: ... 50,000 cells at ∼70% viability were spun down and incubated 3 min at 4°C in 25µl of a detergent buffer containing 0.2% Igepal CA-630 (Sigma, #I8896), 0.2% Tween 20 (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... A Hybond N+ membrane (GE lifesciences, 45000854) and 6 sheets of 3 mm Whatman cellulose paper (Sigma Aldrich, WHA3030861) were pre-soaked for 5 minutes in room temperature 0.5X TBE ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Cell Biology 2020Quote: ... Retinal organoids (n=3-4) for each time point were homogenized using a Dounce Tissue Grinder (Sigma-Aldrich, UK) and processed using SYBR™ Green Fast Advanced Cells-to-CT ™ Kit (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of purified LDL were mixed with 50 µL DiI stock solution (3 mg/mL in DMSO; Sigma) and incubated at 37℃ for 8 h ...
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bioRxiv - Cell Biology 2019Quote: ... glass-bottom dishes (IWAKI, Shizuoka, Japan) were treated with 2% acetic acid (WAKO) and 0.2% 3-(Trimethoxysilyl)propyl methacrylate (SIGMA) in 80% ethanol for 2 min ...
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bioRxiv - Cell Biology 2019Quote: ... Larvae were washed twice in E3 and wounded in a final 0.24 mg/mL tricaine (ethyl 3-aminobenzoate, Sigma)/E3 solution ...
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bioRxiv - Cell Biology 2020Quote: HBEC6-KT and Calu-3 cells were lysed using RIPA Lysis Buffer containing Protease Inhibitor Cocktail powder (Sigma-Aldrich) for 60-90 minutes at 4°C on a rocker ...
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bioRxiv - Immunology 2021Quote: ... 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium (BCIP /NBT) liquid substrates for AP-enzyme (SIGMA) were added for overnight incubation at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed and spots detected by adding a filtered solution of 0.2mg/mL AEC (3-amino-9-ethylcarbazole; Sigma) in 0.1M sodium acetate buffer [pH 5.0] and 0.05% hydrogen peroxide (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Abcam-17785-1-AP) and PSD-95 ((2.5 μg/ml diluted) Abcam-ab12093) using desired concentrations in PBS containing 3% BSA (Sigma), 0.3% Sodium azide (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by selection in 1 μg/mL (C4-2B) or 1.5 μg/mL (DU145, PC-3) of puromycin (Sigma).
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
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bioRxiv - Cell Biology 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-(1’-myo-inositol-4’,5’-bisphosphate) (ammonium salt) (PI(4,5)P2) (Sigma 850155P), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed in PBS and incubated with 3 μg/ml DAPI (4’,6-diamidino-2-phenylindole; D8417 from Sigma Aldrich) at RT for 30min ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times and secondary staining was done in permeabilization buffer supplemented with 5% Donkey serum (Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... and Atto 647N-labeled 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (Atto 647N DPPE, ≤90.0%) were purchased from Sigma-Aldrich. Gibco™ Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated by pipetting (3 μl) or using a pinning tool (Sigma-Aldrich, St. Louis, MO, R2383-1EA) on control and selective media to assess specific phenotypes ...
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bioRxiv - Neuroscience 2021Quote: ... Then mice were randomly infused 10 minutes before the test with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
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bioRxiv - Molecular Biology 2021Quote: ... to 7.0 with HCl) for 45 minutes and a final wash in in 2x SSC (3 M NaCl (Sigma), 0.3 M sodium citrate (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: An RNA oligonucleotide (5’ -UUUUCAUGCUACGCGUAGUUUUCUACGCG- 3’) with Cyanine 5.5 at the 5’-end was obtained from Millipore Sigma (USA). Prior to the reaction ...
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bioRxiv - Developmental Biology 2022Quote: ... amiloride (EIPA) (A3085), LiCl and Sodium chloride (NaCl) (S9888), IPA-3 (I2285, used at 2.5 μM) were obtained from Sigma. Bafilomycin A1 (Baf-A1 ...
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bioRxiv - Cell Biology 2019Quote: ... the grid was washed twice for 3 minutes in washing solution containing filtered PBS and 0.2% glycine (Sigma-Aldrich), blocked for 30 minutes in filtered blocking solution containing ...
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bioRxiv - Immunology 2019Quote: ... Plates were then washed with PBS-T 3 times before the plates were incubated with 75μl Streptavidin peroxidase (1:1000, Sigma) for 60 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... The effect of GABABR antagonists on metamorphosis was tested using Phaclofen (10−3 M, 10−4 M, Sigma Aldrich) and the high affinity antagonist CGP-54626 (10−7 M ...
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bioRxiv - Systems Biology 2019Quote: ... Chk-2 inhibitor II BML-277 (used at 10 μM) from and Nutlin-3 (used at 0.75 - 4 μM, #N6287) from Sigma.
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bioRxiv - Cell Biology 2019Quote: ... Slides were washed 3 times in PBS for 5 min and incubated in DAPI (Sigma Aldrich, Cat. No.10236276001) for nuclei counterstaining ...
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bioRxiv - Immunology 2019Quote: ... Primers were designed using Primer3 software62 with sequences obtained from GenBank and synthesized by Sigma Aldrich (Table 3, 4). PCR reactions were performed on the LightCycler®480 Real Time PCR System (Roche ...
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bioRxiv - Bioengineering 2019Quote: ... The glass micropipettes were surface-modified through silanization with N-(2-aminoethyl)- 3-aminopropyltrimethoxysilane (EDA) (Sigma Aldrich catalog # 440302) in order to reduce stiction forces between polysilicon and the wall of the miniaturized glass micropipette during their integration ...
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bioRxiv - Plant Biology 2019Quote: Treatments with the NO-scavenger 2-(4-Carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazol-1-yloxy-3-oxide potassium salt (c-PTIO salt, Sigma Aldrich, Darmstadt, Germany) were performed 1 hour prior to ethylene treatments to allow for treatment combinations ...
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bioRxiv - Neuroscience 2019Quote: ... 4AP dissolved in ddw to 200 mM (30 μM/L – 3 mM/L; Millipore Sigma, Burlington, MA, cat. #275875) was used to block fast activating fast inactivating potassium currents ...
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bioRxiv - Microbiology 2019Quote: ... The spheroplasts were then washed 3 times in SPM buffer permeabilized by incubation in 1 mL of 0.1% triton X-100 (Sigma) in PBS for 2 minutes on ice and washed twice in PBS ...
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bioRxiv - Immunology 2019Quote: ... The experiment was initiated by adding 3 µCi [9,10−3H]-palmitic acid complexed to 5% BSA (lipids free; Sigma) and ...
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bioRxiv - Evolutionary Biology 2019Quote: ... were toothpicked onto fresh BG11 agar plates containing 0.015 g/L of L-methionine and different concentrations of 3-deazaneplanocin A hydrochloride (Sigma), sinefungin (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... fixed with an ice-cold glutaraldehyde-based solution in CB buffer (0.05 % glutaraldehyde, 3 % formaldehyde and 0.1 % TritonX; Sigma Aldrich) for 15 min on ice ...
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bioRxiv - Cell Biology 2019Quote: ... Larvae were anesthetized prior to experimental procedures in E3 water containing 0.2 mg/ml Tricaine (ethyl 3-aminobenzoate, Sigma). To prevent pigment formation in larvae during imaging experiments ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 3 hr in 5% milk in TBS (50 mM Trizma base and 150 mM NaCl, PH 8.3, both Sigma) plus 0.05% Tween-20 (Sigma ...
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bioRxiv - Immunology 2019Quote: Cell viability was monitored by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT; Sigma, USA) assay as previously described [52] ...