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Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... cells were rinsed once with PBS++ and washed 3 times with PBST (0.1% Triton X (Sigma Aldrich, # T9284) in PBS w/o Ca2+&Mg2+ ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were incubated overnight from 4 dpf in 3 ml 10 mM 5-Bromo-2′-deoxyuridine (B5002, Sigma) with 1% DMSO for 17 hours ...
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bioRxiv - Immunology 2023Quote: ... and dexamethasone (DEX, (11β, 16α)-9-fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3) were obtained from Sigma-Aldrich Korea (Seoul ...
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bioRxiv - Biochemistry 2023Quote: ... Ro 20-1724 [4-(3-butoxy-4-methoxy-benzyl) imidazolidone] was purchased from Sigma Aldrich (catalog no. B8279); stock concentrations were made at 100 mM in DMSO.
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bioRxiv - Neuroscience 2023Quote: Five-month-old C57BL/6 mice (13/group; 10 male and 3 female) were administered dexamethasone (D2915, Sigma; 5mg/kg per day ...
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bioRxiv - Neuroscience 2023Quote: ... and the slices were placed on Millicell membranes (3–4 slices per membrane, 0,4 µm Millicell, Merck Millipore). Slices were cultured in DMEM/F-12 with GlutaMax ...
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bioRxiv - Immunology 2023Quote: ... 4E7 cells were resuspended in 4 mL NK cell expansion media with 3 uM BX-795 (Sigma-Aldrich), and were left to incubate in a 37°C incubator for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... the eluate was sufficiently pure and concentrated using an Amicron Ultra-15 centrifugal filter (3 kDa MWCO, Millipore) to a final concentration of ∼14 g/L ...
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bioRxiv - Physiology 2023Quote: ... both labels were purified using 3 kDa molecular weight cut-off ultrafiltration filter (Amicon Ultra Centrifugal Filter, Millipore). There was no detectable dye in the filtrate for samples ...
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bioRxiv - Synthetic Biology 2023Quote: ... The MTT [3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyl-tetrazolium Bromide] dye (M2128) was obtained from Sigma Aldrich. Triple distilled water (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... and 8% D2O using a Millipore Amicon Ultra-15 Centrifugal Filter Ultracel-3K (Millipore #UFC900324, 3 kDa cutoff), concentrated to a concentration of 50 μM and transferred to an NMR tube after removal of any precipitates by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... washed (at 3,724xg at 40C for 3-5 min) (x3) with PBSx1 using Amicon Ultra-15ml (UFC910024 - Millipore), followed by concentration in storage buffer (described in Materials ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed with 1× PBS and blocked in 3% BSA with 0.1% Triton-X 100 (Thermo-Fisher) and 0.1% Tween (Sigma) in PBS for one hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-3 synthetic gRNAs per gene targeting exonic regions were designed using CCTop54 and ordered from Sigma-Aldrich (spyCas9 sgRNA ...
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bioRxiv - Biophysics 2023Quote: ... RNA was then buffer exchanged into the appropriate buffer using a 3-10 kDa Amicon concentrator (Millipore Sigma).
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bioRxiv - Biophysics 2023Quote: ... cells were blocked and permeabilized with blocking buffer (3% (w/v) bovine serum albumin (BSA, A3059, Sigma-Aldrich), 0.2% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was incubated for 3 h at 22°C in Anti-mono-ADP-Ribose Binding Reagent (Sigma, MABE1076 ...
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bioRxiv - Bioengineering 2023Quote: ... The channel was injected with a freshly prepared solution of 10% (3-Aminopropyl)trimethoxysilane (APTMS, Sigma-Aldrich, #281778) in ethanol and incubated for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: Zebrafish larvae were euthanized in 0.04% MS-222 (Ethyl 3-aminobenzoate methane sulfonic acid salt 98%, Sigma Aldrich) for 15 min and fixed for 30 min at room temperature in 4% paraformaldehyde (PFA) ...
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bioRxiv - Genomics 2023Quote: ... was aliquoted into each of 3 tubes with antibody (1.25 µg anti-HA, Cell Signaling #3724S, 2.5µg anti-ER, Millipore #06-935 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then washed again with TBST (3 × for 10 min each) and developed using TMB (3,3,5,5’-Tetramethylbenzidine, Sigma Aldrich) and hydrogen peroxide for imagining.
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bioRxiv - Microbiology 2023Quote: ... 3 ×104 THP-1 cells were seeded and differentiated by the addition of 5 ng/ml PMA (Sigma) in complete RPMI media (Himedia ...
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bioRxiv - Biochemistry 2022Quote: ... The final supernatant was concentrated to > 1 mg/ml using a 3 kDa centrifugal filter unit (Millipore Sigma) and frozen in aliquots stored at −80°C ...
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bioRxiv - Bioengineering 2023Quote: ... for 20 min at room temperature and then washed 3 times in Phosphate-buffered saline (PBS, Sigma-Aldrich) solution ...
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bioRxiv - Immunology 2023Quote: ... Regeneration down to immobilized sdab026 were achieved with 3×15 second pulses of 10 mm glycine (Sigma Aldrich) at pH 1,5 ...
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bioRxiv - Bioengineering 2023Quote: Monocytes were activated to macrophages by adding 30ng/mL phorbol-12-myristate-3-acetate (PMA; Sigma-Aldrich: P8139) for 24hr ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were dechorionated in 3% sodium hypochlorite and immersed in Halocarbon oil 700 (Sigma Aldrich, 9002-83-9). Then the embryos were imaged after stage 15 when the tracheal tree formed at 3 hours intervals ...
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bioRxiv - Biochemistry 2022Quote: Whole FBS (15 mL) was passed through an Amicon Ultra 3 kDa MW cutoff centrifugal filter (#UFC900308, Millipore) and the resulting ∼15 mL that passed through the filter (corresponding to <3 kDa FBS material ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were counterstained after 3 washes of PBS with 0.3 μg/mL 4,6-diamidino-2-phenylindole (Sigma-Aldrich). Images including Z-stacks were acquired on a Leica SP8 gSTED with an objective with x63 magnification ...
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bioRxiv - Cell Biology 2023Quote: ... Desalted siRNA duplexes modified by double-deoxythymidine overhangs at 3’-ends of both strands were synthesized by Sigma. The AllStar siRNA (QIAGEN ...
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bioRxiv - Developmental Biology 2022Quote: ... Filtered medium was concentrated using Amicon Ultra-15 Centrifugal Filter Unit (3 kDa cut-off; Millipore, cat. # UFC900308) at 5,000 g for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM BME) and concentrated to <4 ml using 100,000 kDa MWCO Amicon® Ultra spin concentrators (Millipore). Protein was loaded on a Superdex 200 pg 16/60 column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed with 1× PBS and blocked in 3% BSA with 0.1% Triton-X 100 (Thermo-Fischer) and 0.1% Tween (Sigma) in PBS for one hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The column was eluted using 6 mL of Strep-start buffer supplemented with 3 mM d-Desthiobiotin (Sigma). The elution was diluted with 20 mL of ion-exchange buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Avanti) and 0.08 mol% Atto488 labeled DPPE (1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine labeled with Atto488, Sigma-Aldrich) with an argon stream followed by desiccation for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined after 72 hours using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (#M5655, Sigma) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and blocked for 1h with 3% goat serum and 1% BSA in PBS/0.25% Triton X-100 (Sigma). The primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then incubated for 2 hours with 3 µg/ml 5-bromo-2’-deoxyurine (Sigma-Aldrich, B5002). Cells were then detached using trypsin/EDTA (GIBCO ...
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bioRxiv - Microbiology 2023Quote: ... 3 PBS washes were performed and blocking was done with 2% Bovine Serum Albumin (Sigma Aldrich,#A7906-100G) and 5% goat serum (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Patch pipettes (3-5 mΩ) were filled with potassium gluconate internal solution (130 mM K-gluconate (Sigma-Aldrich), 11.5 mM Na2-phosphocreatine (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... and blocked for 1 hour at RT in 3% BSA (Applichem) in PBS-Tween buffer (0.01%) (ThermoFisher, Sigma). The membranes were then incubated overnight at 4°C with primary antibodies listed in the Supplemental table 3 with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... The MTT [3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyl-tetrazolium Bromide] dye (M2128) was obtained from Sigma Aldrich. Triple distilled water (Millipore ...
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bioRxiv - Biophysics 2023Quote: ... Samples were prepared in fully deuterated 400 mM SPB for all measurements and contained ∼1 mM 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid (Sigma-Aldrich) as a frequency reference.
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bioRxiv - Cell Biology 2023Quote: ... Cells (or fibers) were then blocked with 3% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) diluted in PBST for 1 hr at room temperature to block non-specific binding sites ...
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bioRxiv - Developmental Biology 2023Quote: ... the L1 larvae were transferred to NGM+auxin plates with 1 mM 3-indoleacetic acid (IAA; Sigma #I2886) and kept in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times and 100 µL per well of SIGMAFAST OPD tablet (#P9187, Sigma-Aldrich, USA) solution was added ...
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bioRxiv - Biochemistry 2023Quote: ... a 3-30% continuous gradient was prepared using Optiprep density gradient medium of 60% iodixanol solution (Sigma-Aldrich) in a buffer composed of 78mM KCl ...
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bioRxiv - Cancer Biology 2023Quote: ... the input media were centrifuged at 3,200 g for 30 min with 3 kDa MWCO filter units (Millipore); after centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... membranes spotted with different lipid species were blocked with 3% fatty acid free BSA (w/v; Sigma, 7030) in 0.1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then blocked in 0.45-μm filtered 3% (w/v) bovine serum albumin (BSA; Sigma-Aldrich A7906) in PBS ...