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Citations for Millipore Sigma :
8851 - 8900 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... were cultured in the same medium conditions with the addition of the 2i chemical inhibitors: 3 μM of CHIR99021 and 250 μM of PD0325901 (Sigma).
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bioRxiv - Synthetic Biology 2021Quote: ... and M9 minimal media supplemented with glucose (6.78 g/L Na2HPO4, 3 g/L KH2PO4, 1 g/L NH4Cl, 0.5 g/L NaCl (Sigma-Aldrich, M6030), 0.34 g/L thiamine hydrochloride (Sigma T4625) ...
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bioRxiv - Cancer Biology 2021Quote: ... after 72 hours of drug treatment, 20μl of a 5mg/mL 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (M2128-MTT; Sigma-Aldrich, USA) solution was added to each of the wells ...
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bioRxiv - Neuroscience 2019Quote: ... washed again 3×10 min in PBS and blocked in PBS with1% bovine serum albumin (BSA) (cat#A7906, Sigma-Aldrich) and 0.1% Triton X-100 for 1h RT ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNAs were concentrated and buffer-exchanged into 10 mM NaHEPES pH 7.0 using Amicon® Ultra-15 centrifugal filter units with 3-kDa molecular weight cutoff (Millipore).
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed with 3% PFA/PBS and the nuclei stained with 4’,6-diamidine-2’-phenylindole (DAPI, Sigma). An inverted fluorescence microscope (Zeiss ...
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bioRxiv - Microbiology 2020Quote: Cells were co-cultured with MA104 cells for 3 days in FBS-free medium supplemented with trypsin (0.5 μg/ml) (Sigma Aldrich) and lysed by freeze-thawing ...
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bioRxiv - Microbiology 2020Quote: ... primers 1022700 5F and R were used to amplify the 572 bp 5’ homology flank and primer pair 1022700 3F and R was used to amplify the 673 bp 3’ homology flank (KOD Hot Start DNA Polymerase, Merck Millipore) which were cloned on either side of the sfGFP expression cassette in pkiwi003 (Ashdown et al. ...
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bioRxiv - Microbiology 2020Quote: ... the bacterial suspension was discarded and tubes were gently washed 3 times with PBS and stained with 0.1% crystal violet (Sigma-Aldrich) for 15 min ...
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bioRxiv - Microbiology 2021Quote: ... mobile phase and stained using ethanol: sulfuric acid (70:3 v/v) solution containing 1% (w/v) orcinol monohydrate (Sigma). HPAEC-PAD samples were diluted to 50 μM and resolved on a Dionex PA20 column in a mobile phase of 30 mM NaOH and gradient of 10 mM – 120 mM sodium acetate over 40 min at a flow rate of 0.5 mL min-1 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Blocking solution was discarded and replaced with 30 mL of 3% BSA TBST buffer then 7.8 µL of 3.85 mg/mL anti-FLAG antibody (Sigma-Aldrich, F3165) was added ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: Human endothelial cells or 3 days post-fertilization (dpf) zebrafish embryos with the yolk sac removed were lysed in RIPA buffer (Millipore) containing protease inhibitors (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... Colonies were fixed with Carnoy’s solution (1 acetic acid: 3 methanol) and stained with 1% (w/v) crystal violet (Sigma-Aldrich). The percentage of colonies covering the plates were calculated with ImageJ software.
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bioRxiv - Biochemistry 2021Quote: ... The cells were co-cultured for 3 days in FBS-free medium supplemented with porcine trypsin (0.5 μg/mL) (Sigma Aldrich). After incubation ...
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bioRxiv - Bioengineering 2021Quote: ... Constructs were then washed in PBS (3 x 5 min) and mounted to glass slides using Fluoroshield (Sigma Aldrich, F6182) mounting medium ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Immunology 2019Quote: ... A total of 300,000 T cells in 200 μl T cell media supplemented with 3-5 μg/ml ConA (Sigma Aldrich) were added to 6.5 mm transwell inserts with 5.0 μm pore size polycarbonate membranes (Corning) ...
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bioRxiv - Immunology 2019Quote: ... A total of 400,000 freshly isolated splenocytes in T cell media supplemented with 3-5 μg/ml ConA (Sigma Aldrich) were added to 6.5 mm transwell inserts with 0.4 μm pore size polyester membranes (Corning ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Microbiology 2020Quote: Caco-2 cells were seeded at 2 × 105 cells/well onto polycarbonate Millicell culture-plate inserts (12-mm diameter, 3-μm pore size; Millipore) and cultured for 5 days ...
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bioRxiv - Neuroscience 2019Quote: ... 150 mM NaCl, 1% Triton-X100, 1X Complete protease inhibitor cocktail [Roche], 1X phosphatase inhibitor cocktails 2 and 3 [Sigma]). Protein concentration was determined using BCA assays (Pierce Biotech).
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]). Samples were centrifuged at 21,000g for 30 min at 25°C to obtain the Triton-insoluble (SDS-soluble ...
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bioRxiv - Genomics 2021Quote: ... at room temperature with a thick layer of self-indicating silica gel (a granular material with c. 3 to 5mm irregular beads; e.g. Type III Sigma-Aldrich, S7625 ...
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bioRxiv - Neuroscience 2019Quote: Non-targeting control siRNA (MISSION® siRNA Universal Negative Control #1) and LRRK2 siRNA #1 (targeting LRRK2 sequence 5’-ctcgtcgacttatacgtgtaa-3’; 37) were purchased from Sigma. LRRK2 siRNA #2 was purchased from ThermoFisher (ID ...
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bioRxiv - Cell Biology 2019Quote: ... and 100 ng/ml M-CSF for 24 h and then selected for expression of the construct with 3 μg/ml puromycin (Sigma) for 24 h ...
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bioRxiv - Immunology 2019Quote: ... Mouse neutrophil was isolated from the peritoneal cavity 3 hours after injection of 1 ml thioglycollate medium (Sigma-Aldrich, MO). Isolated neutrophils were incubated at 37C for 6 hours and then treated with 10 ng/ml ...
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bioRxiv - Cell Biology 2019Quote: ... 5’-GGTCAGCCAACTCGTCACAGTC-3’) and by alizarin red S staining with a 2% (w/v) alizarin red S solution (Sigma-Aldrich). Slides were imaged in an Olympus BX61 microscope ...
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bioRxiv - Neuroscience 2019Quote: ... sections were rinsed 3 times for 2 min in 0.01 M PBS containing 0.2% Triton X-100 (Tx) (PBS-Tx; Sigma-Aldrich, T8787) and then were pre-treated with 3% H2O2 in PBS-Tx for 10 min ...
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bioRxiv - Biochemistry 2019Quote: ... desalted pG was concentrated to a final concentration of 50 µM using Amicon 3 kDa MWCO centrifugal filters (Merck Millipore). 10 nmol lyophilized SMCC-functionalized ODN was reconstituted in 40 µL 50 µM pG (2 nmol ...
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bioRxiv - Bioengineering 2020Quote: Mature brain organoids at 1 to 3-months of age were incubated on polyethyleneimine (PEI) pre-treated MEA plates with 10 μg/mL laminin (Sigma) for 4 hours to ensure attachment to the plate ...
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bioRxiv - Biochemistry 2020Quote: ... the medium was exchanged for differentiation medium I [supplemented DMEM containing 0.5 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma Aldrich), 0.25 μM dexamethasone (Dex ...
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bioRxiv - Bioengineering 2019Quote: ... for 20 min at room temperature (RT) followed by 3 x 5 min washes with PBS/0,1% Triton-X 100 (Sigma-Aldrich). Plates were then incubated with the respective primary antibody in PBS/0,1% Triton-X 100/1% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... larvae were anesthetized in E3 media without methylene blue containing 0.16 mg/ml Tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich). Zebrafish lines used are summarized in Table 1.
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bioRxiv - Cell Biology 2019Quote: The viability of cells was determined using the standard MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (MTT, Sigma-Aldrich, PL). The cells were incubated on 96-well plate (Perkin Merkel ...
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bioRxiv - Neuroscience 2019Quote: hNSG and non-engrafted NSG mice (4-6 months old) were injected 3 mg/kg (i.p.) with lipopolysaccharide (LPS from E. coli; O55:B5, Sigma-Aldrich) or 0.9% saline solution ...
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Systematic Analysis of Lysine Succinylation in Vero cells infected with Small Ruminant MorbillivirusbioRxiv - Genomics 2021Quote: ... The bound peptides were eluted 3 times from the beads with 0.1% trifluoroacetic acid (TFA; Sigma-Aldrich, Saint Louis, USA). The eluted fractions were pooled together and vacuum-dried ...
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bioRxiv - Neuroscience 2020Quote: ... from Tocris CGP-55845 ((2S)-3-[[(1S)-1-(3,4-Dichlorophenyl)ethyl]amino-2-hydro xypropyl](phenylmethyl)phosphinic acid hydrochloride) from Sigma-Aldrich
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bioRxiv - Immunology 2021Quote: ... Cy5- or Cy7-PMPC25-PDPA68 copolymer was dissolved together with the PMPC25-PDPA68 in an organic solution of 3:1 (v/v) methanol:tetrahydrofuran (Sigma-Aldrich) for a final copolymer concentration of 20 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... the bound proteins were eluted by incubation with 250 μl of 3 x FLAG peptide solution (200 μg/mL) (Sigma) for 15-20 min twice ...
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bioRxiv - Cell Biology 2020Quote: ... lysed using cold EZ lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630, Sigma-Aldrich, UK), transferred to chilled nuclease-free tubes ...
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bioRxiv - Genomics 2021Quote: ... Expression of the Dam-POI constructs was suppressed by addition of 0.5 mM indole-3-acetic acid (IAA; Sigma, I5148). Lines were tested routinely for mycoplasma.
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bioRxiv - Biochemistry 2021Quote: ... and transcription factor SREBP1a (aa 1-50) were generated by inserting the corresponding cDNA fragments into pGEX4T-3 vector (Sigma). V5-tagged full-length and N-terminal truncation (Δ302 ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM EDTA) with 0.5 mg/mL lysozyme and 3 mg/mL proteinase K (Sigma-Aldrich Corp., St. Louis, MO). DNA was purified using the PureLink Genomic DNA Mini kit (Thermo Fisher Corp. ...
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bioRxiv - Physiology 2021Quote: ... The preparation was bathed in standard locust saline (in mM: 147 NaCl, 10 KCl, 4 CaCl2, 3 NaOH and 10 HEPES buffer; pH = 7.2; chemicals from Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Auxin-inducible degron (AID)-tagged strains were constructed as described (Snyder et al., 2019) and freshly prepared 10,000x stocks (500 mM) 3-indole acetic acid (IAA) (Sigma Aldrich) dissolved in 100% ethanol were used to treat yeast cultures (YPD ...
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REV-ERBα mediates complement expression and circadian regulation of microglial synaptic phagocytosisbioRxiv - Neuroscience 2020Quote: ... blocked for 30 mins in TBS containing 3% goat serum and 0.25% Triton X-100 (Sigma-Aldrich, St. Louis, MO) then incubated in TBS containing 1% goat serum and 0.25% Triton X-100 with primary antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH, pH7.2, 150 mM NaCl, 500 μg/mL 3×FLAG peptide [Sigma-Aldrich]). The final supernatant containing 3×FLAG-TBC1D18 was transferred to a fresh tube.
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bioRxiv - Systems Biology 2021Quote: INS-1 cells (INS-1 832/3 rat insulinoma cell line) were cultured in RPMI-1640 (Sigma Cat. No. R0883) supplemented with 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2020Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... 2-3 mg of sNASP-FLAG-myc and sNASP-Strep-HA extracts were incubated for 3 hours at 4 °C degrees with 50 μl of anti-Flag M2 (A2220, Sigma) or Anti-TwinStrep Mag-Strep beads (2-4090-002 ...