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Citations for Millipore Sigma :
9101 - 9150 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: 15 dpi nodules were hand sectioned and kept for 10 minutes in staining solution (1:3 ratio of 1:500 dilution of propidium Iodide (Sigma) and 1 ...
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bioRxiv - Neuroscience 2023Quote: ... Culture supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Immunology 2023Quote: ... capsules were attached to the back of mice using a warmed adhesive solution made from 3 parts gum rosin (Millipore- Sigma) and 1 part beeswax (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... Twenty-four hours after transduction cells were recovered for another 24 hrs and then MCF10A were selected in 3 μg/ml puromycin dihydrochloride (Sigma) for 48 hrs or 10 μg/ml blasticidin (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...
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bioRxiv - Immunology 2023Quote: ... 3 dpf larvae were anesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The corresponding concentration of bacteria was injected into the corresponding part of the zebrafish ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 100 µL of elution buffer supplemented with 200 µg/mL 3× Flag-peptide (Sigma-Aldrich, #F4799) and stored at -80◦C until usage ...
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bioRxiv - Immunology 2023Quote: ... BMDMs were primed with 100 ng/mL LPS for 3 hours followed by 2.5 mM ATP treatment or 5h 10uM IKKi (BMS-345541, Sigma-Aldrich) treatment ...
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bioRxiv - Cell Biology 2023Quote: ... 3 – 6 mg of cell extract and 20 μl of α-Flag M2 affinity bead suspension (Sigma, catalog no.: A2220) were used ...
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bioRxiv - Developmental Biology 2023Quote: ... in PBS at +4⁰C in the shaker and then 3-4 min with a pancreatin-trypsin (2.5 mg/ml pancreatin [P3292; Sigma Aldrich] and 22.5 mg/ml trypsin dissolved in Thyrode’s solution pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... 3 µL of 2 M CaCl2 and 5 µL of 10 mg/mL ε-aminocaproic acid (ε-ACA) (Sigma-Aldrich), and 1 µL of 1U/µL Thrombin (MP Biomedicals ...
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bioRxiv - Immunology 2023Quote: ... mice were fasted for four hours before oral gavage of 600 mg/kg FITC–3-5-kDa dextran (Sigma, FD4). Serum was collected six hours after gavage as well as control mice without gavage and analyzed using a Synergy HT plate reader (Bio-Tek ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was freshly prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ∼20 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were also washed for 10 minutes in a solution of 1.25×10−3 mg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Sigma: D9542) in PBS to fluorescently label nuclei within tissue sections.
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bioRxiv - Cell Biology 2023Quote: Fresh tumor-free lung tissue was filled with a 3% (w/v) low melting agarose preparation media (Sigma, A9414-100G). Agarose filling was performed via a catheter inserted through one or several identified bronchi ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... MSCs were frozen at passage 3 with 1.25×106 cells/ml in freezing medium containing fetal bovine serum (FBS BCBV7611, Sigma-Aldrich) with 10% dimethylsulfoxide (DMSO ...
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bioRxiv - Bioengineering 2023Quote: ... the dermis construct was consolidated in a solution composed of 3% (w/v) calcium chloride (Calcium Chloride, Sigma Aldrich, C5670) and Thrombin at a concentration of 10 U/mL (Thrombin from bovine plasma ...
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bioRxiv - Biochemistry 2022Quote: ... The final solution was washed 3 times with 1X PBS using an Amicon Ultra 30 kDa MW cutoff centrifugal filter (#UFC903024, Millipore) to remove unreacted PMSF and then resuspended to 200 µL (same volume as original sample) ...
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bioRxiv - Cell Biology 2022Quote: Activation of the Cre recombinase was induced in controls and the various Knockouts mice by daily intraperitoneal (i.p.) injections of 3 mg/kg/day of tamoxifen (TMX, Sigma T5648) for 5 consecutive days ...
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bioRxiv - Cell Biology 2023Quote: ... composed of Egg yolk L-α-Phosphatidylcholine (EyPC, Avanti Polar), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS, Avanti Polar) and Cholesterol (Sigma) (50:20:30 ...
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bioRxiv - Biophysics 2023Quote: ... a 10−3 M stock solution of TPPS4 was prepared by dissolving the powder (Sigma Aldrich, St. Louis, Missouri, USA) in distilled water ...
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bioRxiv - Genetics 2022Quote: ... cells were grown in a basal erythroid medium (BEM) supplemented with hSCF, IL-3, EPO (Eprex, Janssen-Cilag) and hydrocortisone (Sigma). From day 6 to 20 ...
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bioRxiv - Genomics 2023Quote: ... Frozen cells were thawed and added to a Dounce homogenizer containing 1 mL of nuclei isolation buffer (NIB: 10 mM TrisHCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal (Millipore Sigma #18896), and 1 x protease inhibitors (Millipore Sigma #118735800001)) ...
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bioRxiv - Immunology 2023Quote: ... Mice were maintained on a purified diet (‘AhR-poor diet’, AIN-93M, Safe diets) supplemented or not in Indole-3-carbinol (I3C, 200 ppm, Sigma). In some experiments ...
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bioRxiv - Cell Biology 2023Quote: ... cells were inoculated in YEP containing 3% lactic acid and treated with a final concentration of 2% galactose (Sigma-Aldrich). For the inducible degradation of the proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... The growth medium of organoids was supplemented with 100μM with 8-Bromoadenosine 3’,5’-cyclic monophosphate sodium salt (8-Br-cAMP) (Sigma, #B7880) in iPSC organoids or 200μM forskolin (FSK ...
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bioRxiv - Molecular Biology 2023Quote: ... Cumulus oocyte complexes were collected by tearing the ampulla wall of the oviduct, placed in Ferticult medium (FertiPro N.V, Belgium) supplemented with 3 % BSA (Sigma–Aldrich), and maintained at 37°C under 5 % CO2 in air under mineral oil (FertiPro N.V ...
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bioRxiv - Genetics 2023Quote: ... Liquid nitrogen-frozen pellets equivalent to 1 g of animals were homogenised in 3 ml of lysis buffer (Sigma-Aldrich, L3412 ...
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bioRxiv - Microbiology 2023Quote: ... and blocked for 1 hour in PBS containing 0.1% w/v saponin-3% bovine serum albumin (BSA, Sigma Aldrich, A7906). Samples were again rinsed three times with PBS and incubated with primary antibodies as indicated in Table S5D ...
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bioRxiv - Microbiology 2023Quote: ... Protein was then eluted off the agarose by resuspension in 100 µL of 3X FLAG elution buffer (3 µL of 5 mg/mL Sigma 3X FLAG peptide solution + 97 µL of coIP buffer 2 ...
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bioRxiv - Immunology 2023Quote: ... ex vivo isolated naïve or overnight stimulated (anti-CD40 at 5μg/ml and IL-4 at 1ng/ml) 3×105 B cells were plated on a poly-D lysine (PDL) (Sigma)-coated XF96 cell culture microplate and incubated at 37 °C for a minimum of 30 min in a CO2-free incubator in assay medium (XF RPMI medium pH 7.40 supplemented with 2 mM L-glutamine ...
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bioRxiv - Plant Biology 2023Quote: ... The 30 collected fractions were combined into 15 pools and desalted on C18 Cartridges (Empore SPE Cartridges C18 standard density, bed I.D. 7 mm, volume 3 ml, Sigma-Aldrich). Each fraction was concentrated by vacuum centrifugation and reconstituted in 40 µl of 0.1% (v/v ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Cell Biology 2023Quote: Lipid overlay assay PI strips were blocked in PBST (0.1% Tween 20 [v/v]) supplemented with 3% (w/v) fatty acid-free BSA (Sigma-Aldrich) overnight at 4°C and then incubated with 0.5 μg/ml GST fusion proteins for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... diluted to 20 µg mL-1 in 10 mM Tris-SO4 (pH 7.5) and 250 mM sucrose and supplemented with 3 µM horse heart cytochrome c (Sigma Aldrich). For measurement of NADH:O2 oxidoreduction ...
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bioRxiv - Bioengineering 2023Quote: iLPC organoids formed after 48 hours post-seeding were fixed for 3 hours in 4% paraformaldehyde (PFA) in phosphate buffered saline (PBS) (Sigma), before a double-embedding process in 1.5% agarose gel then paraffin as described previously [13 ...
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bioRxiv - Cell Biology 2023Quote: ... The calvarias were shredded to 1 mm × 1 mm pieces and digested for 30 min at 37 °C in Dulbecco’s modifiation of Eagle’s medium (DMEM, Gibico, USA) with 3 mg/ml collagenase I (Sigma-Aldrich, Germany) and 0.25% trypsin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were again washed 3 × 5 min with PBS + 0.1% Tween 20 and incubated with Classico/Crescendo Western HRP substrate (Millipore-Sigma) for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was then concentrated to 100-200 μL using centrifugal filter units with a 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) by centrifugation at 5000 g at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Purified proMMP-3 and mutants were activated for 16 h in the presence of 1 mM 4-amino-phenyl mercuric acetate (Sigma) at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Then the brains were dissected to 2 mm slices and incubated for 10 minutes in freshly prepared 0.5% TTC solution (2, 3, 5-Triphenyltetrazolium chloride, Sigma-Aldrich). Afterward ...
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bioRxiv - Molecular Biology 2023Quote: ... Preparative scale reactions were carried out in 3 mL Slide-A-Lyzer cassettes (10 kDa MWCO, Merck Millipore, Darmstadt, Germany) in combination with custom-made FM containers57 ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... n = 3 – 9 mice / group) were transferred to a 1.5 mL microcentrifuge tube and washed with pre-warmed PBS+/+ (Sigma, # D8662). Islets were dispersed by adding 400 µl Accutase (VWR ...
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bioRxiv - Cancer Biology 2023Quote: ... two to four-month-old mice were gavaged 3 times over 5 days with 2 mg tamoxifen (TX; Sigma #T5648) in corn oil (Sigma #C8267) ...
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bioRxiv - Genomics 2023Quote: ... Expression of the Dam-POI in the mouse ESC lines was suppressed by addition of 0.5 mM indole-3-acetic acid (IAA; Sigma, I5148).
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bioRxiv - Cell Biology 2023Quote: ... when embryoid bodies were formed) half of the medium was replaced with differentiation medium supplemented with 3 μM IWR-1-Endo (Sigma), 1 μM Dorsomorphin (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mm3 tissue pieces from 2-3 brains were pooled and enzymatically digested in a dissociation medium containing 0.16 mg/mL cysteine (Sigma C9768), 7 U/mL Papain (Sigma P3125) ...
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bioRxiv - Pathology 2023Quote: Mouse epidydimal adipose tissue was harvested and digested in Hanks’ Balanced Salt Solution (HBSS, no calcium and magnesium) containing fetal bovine serum (FBS,3%) and collagenase D (2.5 mg/ml, Sigma-Aldrich) for 30 minutes at 37°C under agitation ...
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bioRxiv - Neuroscience 2023Quote: Cells were fixed in 4% paraformaldehyde and permeabilized/blocked using PBS with 3% goat or donkey serum and 0.2% triton X-100 (Sigma Aldrich). Cells were incubated at 4°C overnight with primary antibodies against the following targets ...